[Study on therapeutic dosimetry of HIFU ablation tissue].

It is a difficult problem in high intensity focused ultrasound (HIFU) therapeutic dosimetry that how to use a BFR to ablate a mass in tissue and to determine the energy-efficiency relation, that is, the scale of biological effects of HIFU. A mass lesion was realized in this study according to a treatment principle of damaging tissue from BFRs to fascicle lesions, slice lesions and a mass lesion. A 1.

Recombinant M. smegmatis vaccine targeted delivering IL-12/GLS into macrophages can induce specific cellular immunity against M. tuberculosis in BALB/c mice.

In the present study, we constructed a viable therapeutic vaccine of recombinant M. smegmatis mediated IL-12/GLS (granulysin) gene transfer into murine macrophages to exert the immunotherapy effects on the Mycobacterium tuberculosis infection. We tested this recombinant therapeutic vaccine in an in vivo study to determine its capability of stimulating host specific immune responses against M.

Effect of APOE polymorphisms on early responses to traumatic brain injury.

To investigate the relationship between apolipoprotein E (APOE) polymorphisms and the severity of traumatic brain injury (TBI) in acute stage in the cohort of mainland Chinese patients. We prospectively identified admissions to the two neurosurgical departments for head injury. A total of 110 subjects with TBI (80 males and 30 females, with mean age of 43.

[Inhibition of HBV replication and antigen expression by RNA interference against different targets].

Objectives: To observe the inhibition of HBV replication and antigen expression by RNA interference aimed at different parts of the HBV genome.

Methods: Following the rules of shRNA expression vector design and construction, we constructed seven kinds of sequence specific vectors and two kinds of mutant shRNA expression ones. We then cotransfected those shRNA and HBV expression vectors into HepG2 cells using lipofectamine2000.

Real-time quantitative assay of HCV RNA using the duplex scorpion primer.

A novel real-time quantitative method for detecting HCV in serum was established in which the duplex scorpion primer was used to provide a unimolecular probing mechanism for hybridizing the highly conserved 5' noncoding region (5' NCR) of the HCV genome specifically. Through methodological evaluation, we found this new method had a wide linearity, high sensitivity, repeatability and specificity. Compared to the commercial TaqMan method, this method was found to be more sensitive and less costly, and the final results were obtained more quickly.

[Treatment of murine Lewis lung cancer with recombinant interleukin-12].

Background & Objective: Interleukin-12 (IL-12) is a proinflammatory cytokine with antitumor activity. The study was to compare the therapeutic effects of recombinant murine interleukin-12 (mIL-12) plasmid [pcDNA3.1(+)-mIL-12] and Lewis lung carcinoma (LLC) cell line which could stably express mIL-12 gene in LLC.

[Influence of bifidobacterial whole peptidoglycan on cellular immune response induced by recombinant HBsAg].

Aim: To explore the effect of bifidobacterial whole peptidoglycan (BWPG) on cellular immune response to recombinant hepatitis B surface antigen (rHBsAg).

Methods: BWPG was used as an adjuvant of rHBsAg to immunize BALB/c mice. The growth state and development of central lymphoid organ of immunized mice were observed.

[The protection mechanisms of glycine against liver injury induced by lipopolysaccharides].

Objective: To explore the protective mechanisms of glycine (Gly) on lipopolysaccharides (LPS) induced liver injury.

Methods: BABL/c mice were randomly divided into a LPS group, in which the animals were intraperitoneally injected with 10 mg/kg LPS, and a Gly group, in which the mice were pretreated with a 5% Gly-containing diet for 3 days before receiving the same dose of LPS. The livers of the mice were examined for histopathological changes.

[Screening of proteins interacting with hepatitis C virus NS3 from T7-phage display library].

Objective: To screen and identify proteins that interact with hepatitis C virus NS3 by means of T7-phage display system.

Methods: Hepatitis C virus NS3 was expressed by prokaryotic expression and used as a selected molecule to biopan the T7 select human liver cDNA library; the selected positive clones were identified using DNA sequencing and analyzed with BLAST program in GenBank.

Results: After BLAST analysis in all the positive clones, the proteins which interacted with the hepatitis C virus NS3 were found to be serpin peptidase inhibitor, clade A, member 1 (SERPINA1) and cyclophilin-LC.

[An analysis of the impact factors of hepatology journals in SCI].

Objective: To analyze the status of liver disease research in China.

Method: Hepatology and gastroenterology journals in the Science Citation Index were searched and their impact factors were analyzed.

Results: 19 periodicals in this field were published in 6 countries.

[Citation analysis of the Chinese Journal of Hepatology (Zhonghua Ganzangbing Zazhi) in the year 2005].

Objective: To evaluate the academic level and the popularity of the Chinese Journal of Hepatology in China in 2005.

Methods: We used bibliometrics to analyze statistically the original articles of the Chinese Journal of Hepatology cited by Chinese periodicals included in the Wanfang Data in 2005.

Results: (1) 699 published papers in the journal in 2005 were cited 1673 times and 44 of them were cited 720 times in total.

Decreased expression of thyroid receptor-associated protein 220 in temporal lobe tissue of patients with refractory epilepsy.

Purpose: TRAP220 (thyroid hormone receptor-associated protein) functions as a coactivator for nuclear receptors and stimulates transcription by recruiting the TRAP mediator complex to hormone responsive promoter regions. Thus, TRAP220 enhances the function of thyroid/steroid hormone receptors such as thyroid hormone and oestrogen receptors. This study investigated the expression of TRAP220 mRNA and protein level in epileptic brains comparing with human control.

Protective effects of meloxicam on aluminum overload-induced cerebral damage in mice.

The putative protective effects of meloxicam on the oxidative damage induced by aluminum overload in mice brain were investigated. The cerebral damage model in mice was established via intracerebroventricular (i.c.

[The relationship between histone acetylation modification and IFN-gamma responsive gene regulation].

Objectives: To study the role of histone modification in the regulation of IFN-gamma-activated gene using chromatin immunoprecipitation technique.

Methods: Real-time PCR was used to measure the mRNA level of interferon-gamma-inducible protein 10 (IP-10) in Hela cells. Chromatin immunoprecipitation combined with Real-time PCR was used to check the histone H4 acetylation level at IFN-stimulated response element (ISRE) locus of IP-10 gene.

[Inhibition of HCV IRES controlled reporter gene expression by RNA interference].

Objective: To develop a RNAi approach that specifically targets the HCV IRES sequence by vector-expressed short hairpin RNA (shRNA) in vitro, and to assess the inhibitory effect of the shRNA on reporter gene expression.

Methods: Eukaryotic expressing plasmids, pIRES-GFP and p5' UTR-Luc containing GFP or luciferase gene controlled by HCV IRES were cotransfected into HepG2 cells with either a RNAi plasmid pshRNA-HCV or a control plasmid pTZU6+1. At 24, 48, 72 hours post transfection, the fluorescence in the transfected cells was studied using fluorescence microscopy.

Photodynamic effects of pyropheophorbide-a methyl ester in nasopharyngeal carcinoma cells.

Background: Nasopharyngeal carcinoma (NPC) is one of the most common cancers, and exploring novel therapeutic modalities will improve the clinical outcomes. It has been confirmed that photodynamic therapy can efficiently deactivate malignant cells. The aim of the present study was to explore the photodynamic effects of pyropheophorbide-a methyl ester (MPPa) in CNE2 nasopharyngeal carcinoma cells.

[Effects of pshRNA-DNMT1 on the proliferation and apoptosis of gastric cancer: experiments in vitro and in vivo].

Objective: To investigate the effects of pshRNA-DNMT1 on the proliferation and apoptosis of gastric cancer.

Methods: Recombinant eukaryotic expression plasmid pshRNA-DNMT1 containing the sequence of the gene of DMNT1 that methylates the specific pyrimidine residue in the DNA promoter region was constructed. Human gastric cells of the line AGS were cultured and transfected with pshRNA-DNMT1.

[Combined effects of neonatal Bacillus Calmette-Guerin vaccination and respiratory syncytial infection on experimental asthma in mice].

Objective: Neonatal Bacillus Calmette-Guerin (BCG) vaccination could decrease asthma prevalence in human according to "hygiene hypothesis". The authors proposed a hypothesis that effect of BCG vaccination on inhibiting asthma in human might be reversed by respiratory virus infection. The objective of this study was to observe combined effects of neonatal BCG vaccination and respiratory syncytial virus (RSV) infection on experimental asthma in mice.

Therapeutic DNA vaccines against tuberculosis: a promising but arduous task.

Objective: To review recent developments in therapeutic DNA vaccines against tuberculosis.

Data Sources: The data used in this review were obtained mainly from the studies of therapeutic DNA vaccines against tuberculosis reported from 2000 to 2006.

Study Selection: Relevant articles about studies of therapeutic DNA vaccines against tuberculosis were selected.

[Effect of silencing of hyaluronidase gene HYAL1 by RNA interference on proliferation of human breast cancer cells].

Background & Objective: It is reported that hyaluronidase is related to malignant potentiality of human breast cancer. This study was to investigate whether HYAL1 RNA interference (RNAi) could effectively inhibit gene mRNA expression as well as cell proliferation in human breast cancer cells.

Methods: Chemically synthesized double stranded RNA (dsRNA) targeting HYAL1 was transfected into human breast cancer cell lines MDA-MB-231, MDA-MB-453S, ZR-75 and ZR-75-30 using SiPORT lipid.

[Effect and possible mechanism of HnRNP E2 decoy RNA on proliferation of K562 leukemia cells].

Background & Objective: The abnormal expression of poly(rC)-binding protein E2 (hnRNP E2) induced by BCR/ABL plays an important role in blast crisis of chronic myeloid leukemia (CML). The present study was to investigate the effect of hnRNP E2 decoy RNA on the cell proliferation in K562 leukemia cells, and further elucidate the possible underlying mechanisms.

Methods: Decoy hnRNP E2 plasmid was constructed and transfected into K562 cells using cationic liposome.

Prospective randomized study comparing amiodarone vs. amiodarone plus losartan vs. amiodarone plus perindopril for the prevention of atrial fibrillation recurrence in patients with lone paroxysmal atrial fibrillation.

Aims: The purpose of this trial was to compare the long-term efficacy of low-dose amiodarone with losartan and perindopril (both combined with low-dose amiodarone) for the prevention of atrial fibrillation (AF) recurrence in patients with lone paroxysmal AF.

Methods And Results: One-hundred and seventy-seven patients with lone paroxysmal AF were randomly assigned to three treatment groups: group 1 received low-dose amiodarone alone, group 2 received low-dose amiodarone plus losartan, and group 3 received low-dose amiodarone plus perindopril. Left atrial diameter was measured with transthoracic echocardiogram at baseline and 6, 12, 18, and 24 months after randomization.