[Study on inducing an artemisinin-resistant line of Plasmodium berghei].

Objective: To induce a line of Plasmodium berghei with resistance to artemisinin.

Methods: The major methods included blood transmission from passage to passage and progressive increase of drug pressure.

Results: The resistant lines were developed by different protocols: (A) The initial dosage of artemisinin was 126.

[Sequence difference of ribosomal DNA second internal transcribed spacer in Anopheles minimus in different localities].

Objective: To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in Anopheles minimus from Yunnan, Hainan, Guangxi of China and from Thailand.

Methods: Single mosquito's legs were digested to extract DNA; the rDNA-ITS2 region was amplified by specific primers and the PCR products were purified, sequenced and analysed.

Results: Two different kinds of ITS2 sequences, temporarily named as An.

[Study on molecular phylogeny of Schistosoma sinensium based on mitochondrial genes].

Objective: To determine the phylogenetic position of Schistosoma sinensium in the genus Schistosoma using mitochondrial cytochrome C oxidase 1 (CO1) and NADH dehydrogenase 1(ND1) as molecular markers.

Methods: The genomic DNA of adult worms were extracted by the GNT-K method. The target regions were amplified by PCR using specific primers.