Chiral Amino Acids Mediate Mitochondria-Dependent Apoptosis of Human Proximal Tubular Epithelial Cells Under Oxidative Stress.

Amino acids are the basic structural units of life, and their intake levels affect disease and health. In the case of renal disease, alterations in amino acid metabolism can be used not only as a clinical indicator of renal disease but also as a therapeutic strategy. However, the biological roles and molecular mechanisms of natural chiral amino acids in human proximal tubular epithelial cells (HK-2) remain unclear.

Inflammatory Effects and Regulatory Mechanisms of Chitinase-3-like-1 in Multiple Human Body Systems: A Comprehensive Review.

Chitinase-3-like-1 (Chi3l1), also known as YKL-40 or BRP-39, is a highly conserved mammalian chitinase with a chitin-binding ability but no chitinase enzymatic activity. Chi3l1 is secreted by various cell types and induced by several inflammatory cytokines. It can mediate a series of cell biological processes, such as proliferation, apoptosis, migration, differentiation, and polarization.

Utilizing the Fungal Bicistronic System for Multi-Gene Expression to Generate Insect-Resistant and Herbicide-Tolerant Maize.

Developing simple and efficient multi-gene expression systems is crucial for multi-trait improvement or bioproduction in transgenic plants. In previous research, an -based bicistronic system from the nonpathogenic fungus efficiently expressed multiple enzyme proteins in yeast and maize, and the heterologous enzymes successfully performed their catalytic activity to reconstruct the biosynthetic pathway in the host organism. Unlike enzyme proteins, some heterologous functional proteins (such as insecticidal proteins) are dose-dependent and they need to express sufficient levels to perform their biological functions.

Identification, Phylogeny, and Expression Profiling of Pineapple Heat Shock Proteins (HSP70) Under Various Abiotic Stresses.

Pineapple ( (L.) Merr.) is an economically significant and delicious tropical fruit.

Integrated Analysis of Transcriptome and Metabolome in the Brain After Cold Stress of Red Tilapia During Overwintering.

Cold stress during overwintering is considered a bottleneck problem limiting the development of the red tilapia ( spp.) industry, and the regulation mechanism is currently not well understood. In this study, the fish (initial weight: 72.

Depletion of Gibberellin Signaling Up-Regulates Transcription and Promotes Adventitious Root Formation in Leaf Explants.

Adventitious root (AR) formation in plants originates from non-root organs such as leaves and hypocotyls. Auxin signaling is essential for AR formation, but the roles of other phytohormones are less clear. In , at least two distinct mechanisms can produce ARs, either from hypocotyls as part of the general root architecture or from wounded organs during de novo root regeneration (DNRR).

Expanding Horizons of CRISPR/Cas Technology: Clinical Advancements, Therapeutic Applications, and Challenges in Gene Therapy.

CRISPR-Cas technology has transformed the field of gene editing, opening new possibilities for treatment of various genetic disorders. Recent years have seen a surge in clinical trials using CRISPR-Cas-based therapies. This review examines the current landscape of CRISPR-Cas implementation in clinical trials, with data from key registries, including the Australian New Zealand Clinical Trials Registry, the Chinese Clinical Trial Register, and ClinicalTrials.

Stress on the Endoplasmic Reticulum Impairs the Photosynthetic Efficiency of Chlamydomonas.

Stress on the Endoplasmic reticulum (ER) can severely disrupt cellular function by impairing protein folding and post-translational modifications, thereby leading to the accumulation of poor-quality proteins. However, research on its impact on photosynthesis remains limited. In this study, we investigated the impact of ER stress on the photosynthetic efficiency of Chlamydomonas reinhardtii using pharmacological inducers, tunicamycin (TM) and brefeldin A (BFA), which specifically target the ER.

Evolution and Function of MADS-Box Transcription Factors in Plants.

The MADS-box transcription factor (TF) gene family is pivotal in various aspects of plant biology, particularly in growth, development, and environmental adaptation. It comprises Type I and Type II categories, with the MIKC-type subgroups playing a crucial role in regulating genes essential for both the vegetative and reproductive stages of plant life. Notably, MADS-box proteins can influence processes such as flowering, fruit ripening, and stress tolerance.

Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture.

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) is an innovative cancer treatment method that has received considerable scientific attention. The objective of this study is to evaluate the effects of PAM on the anti-tumor characteristics of non-small cell lung cancer (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures.

Genome-Wide Identification of the Trihelix Transcription Factor Family and Functional Analysis of in Maize.

The trihelix transcription factor, which is a plant-specific family, play a critical role in plant growth and development and stress responses. Drought is the main limiting factor affecting yield of maize (). However, the identification and characterization of this gene family in maize and its biological functions in response to drought stress have not been reported.

Young Goji Fruit Volatiles Regulate the Oviposition Behavior and Chemosensory Gene Expression of Gravid Female .

The goji fruit fly, , is a major pest on the well-known medicinal plant . Dissecting the molecular mechanisms of the oviposition selection of regarding the host plant will help to identify new strategies for pest fly control. However, the molecular mechanism of chemical communication between the goji fruit fly and the host goji remains unclear.

Risk Prediction and Management for Central Nervous System Infection After Resection for Gliomas-The 8-Year Experience from a Tertiary Medical Center.

: To identify risk factors for central nervous system infection (CNSI) following glioma resection and develop a predictive model. : Retrospective analysis of 435 glioma resection cases was conducted to assess CNSI risk factors. A nomogram predictive model was constructed and validated internally and externally.

Identification of miRNAs Involved in Olfactory Regulation in Antennae of Beet Webworm, (Lepidoptera: Pyralidae).

The beet webworm, , is a typical migratory pest. Although miRNAs participate in many physiological functions, little is known about the functions of miRNAs in olfactory regulation. In this study, 1120 (869 known and 251 novel) miRNAs were identified in the antennae of by using high-throughput sequencing technology.

The Expression Profile of Genes Related to Carotenoid Biosynthesis in Pepper Under Abiotic Stress Reveals a Positive Correlation with Plant Tolerance.

In light of the increasingly adverse environmental conditions and the concomitant challenges to the survival of important crops, there is a pressing need to enhance the resilience of pepper seedlings to extreme weather. Carotenoid plays an important role in plants' resistance to abiotic stress. Nevertheless, the relationship between carotenoid biosynthesis and sweet pepper seedlings' resistance to different abiotic stresses remains uncertain.

Genome-Wide Identification and Characterization of Gene Family in (Cucurbitaceae).

is a traditional Chinese medicinal plant of considerable application value and commercial potential, primarily due to its production of various bioactive compounds, particularly dammarane-type triterpenoid saponins that are structurally analogous to ginsenosides. Oxidosqualene cyclase (OSC), a pivotal enzyme in the biosynthesis of triterpenoid metabolites in plants, catalyzes the conversion of oxidosqualene into triterpenoid precursors, which are essential components of the secondary metabolites found in . To elucidate the role of gene family members in the synthesis of gypenosides within , this study undertook a comprehensive genome-wide identification and characterization of genes within and compared their expression levels across populations distributed over different geographical regions by both transcriptome sequencing and qRT-PCR experimental validation.

of Positively Regulate Thermotolerance by Transcriptionally Activating and .

Heat shock transcription factors (HSFs) are crucial components in heat stress response. However, the contribution of the HSFs governing the inherent thermotolerance in has barely been investigated. We here compared the roles of , , and in heat stress tolerance.

Correction: Yu et al. The Role of circTmeff-1 in Morphine Addiction Memory of Mice. 2023, , 1985.

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Structure of the WIPI3/ATG16L1 Complex Reveals the Molecular Basis for the Recruitment of the ATG12~ATG5-ATG16L1 Complex by WIPI3.

Macroautophagy deploys a wealth of autophagy-related proteins to synthesize the double-membrane autophagosome, in order to engulf cytosolic components for lysosome-dependent degradation. The recruitment of the ATG12~ATG5-ATG16L1 complex by WIPI family proteins is a crucial step in autophagosome formation. Nevertheless, the molecular mechanism by which WIPI3 facilitates the recruitment of the ATG12~ATG5-ATG16L1 complex remains largely unknown.

Complex Large-Deformation Multimodality Image Registration Network for Image-Guided Radiotherapy of Cervical Cancer.

In recent years, image-guided brachytherapy for cervical cancer has become an important treatment method for patients with locally advanced cervical cancer, and multi-modality image registration technology is a key step in this system. However, due to the patient's own movement and other factors, the deformation between the different modalities of images is discontinuous, which brings great difficulties to the registration of pelvic computed tomography (CT/) and magnetic resonance (MR) images. In this paper, we propose a multimodality image registration network based on multistage transformation enhancement features (MTEF) to maintain the continuity of the deformation field.

Population and Co-Occurrence Characteristics of Diagnoses and Comorbidities in Coronary Artery Disease Patients: A Case Study from a Hospital in Guangxi, China.

Coronary artery disease (CAD) remains a major global health concern, significantly contributing to morbidity and mortality. This study aimed to investigate the co-occurrence patterns of diagnoses and comorbidities in CAD patients using a network-based approach. A retrospective analysis was conducted on 195 hospitalized CAD patients from a single hospital in Guangxi, China, with data collected on age, sex, and comorbidities.

The Effect of Conditioned Medium from Angiopoietin-1 Gene-Modified Mesenchymal Stem Cells on Wound Healing in a Diabetic Mouse Model.

Introduction: Mesenchymal stem cells (MSCs) have been introduced as a promising treatment for diabetic wounds. The effects of stem cell therapy are thought to be caused by bioactive molecules secreted by stem cells. Stem cell-based gene therapies can target bioactive molecules.

HSF-IBI: A Universal Framework for Extracting Inter-Beat Interval from Heterogeneous Unobtrusive Sensors.

Heartbeat inter-beat interval (IBI) extraction is a crucial technology for unobtrusive vital sign monitoring, yet its precision and robustness remain challenging. A promising approach is fusing heartbeat signals from different types of unobtrusive sensors. This paper introduces HSF-IBI, a novel and universal framework for unobtrusive IBI extraction using heterogeneous sensor fusion.

Non-Contact Stable Arterial Pulse Measurement Using mmWave Array Radar.

Pulse signals can serve as important indicators of one's cardiovascular condition. However, capturing signals with stable morphology using radar under varying measurement periods remains a significant challenge. This paper reports a non-contact arterial pulse measurement method based on mmWave radar, with stable signals achieved through a range-angle focusing algorithm.

A Dual-Branch Residual Network with Attention Mechanisms for Enhanced Classification of Vaginal Lesions in Colposcopic Images.

Vaginal intraepithelial neoplasia (VAIN), linked to HPV infection, is a condition that is often overlooked during colposcopy, especially in the vaginal vault area, as clinicians tend to focus more on cervical lesions. This oversight can lead to missed or delayed diagnosis and treatment for patients with VAIN. Timely and accurate classification of VAIN plays a crucial role in the evaluation of vaginal lesions and the formulation of effective diagnostic approaches.