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Co-proliferation of antimicrobial resistance genes in tilapia farming ponds associated with use of antimicrobials.

Sci Total Environ

August 2023

School of Public Health, The University of Queensland, Herston, QLD 4006, Australia; School of Population Health, University of New South Wales, Sydney, NSW 2052, Australia; European Centre for Environment and Human Health, University of Exeter, Truro TR1 3HD, UK.

The prevalence of antimicrobial resistance genes (ARGs) in aquaculture has raised serious public concerns for food safety and human health, but its relationships to the use of antimicrobials in aquacultural ponds and even to their residues in the whole aquatic environment remain unclear. In this study, a better coverage of 323 target ARGs and 40 mobile genetic elements (MGEs) was analyzed in sediment using a smart chip-based high-throughput quantitative PCR approach (HT-qPCR) in random 20 ponds of a tilapia farming base in southern China, whose antimicrobial residues were reported previously. In total, 159 ARGs and 29 MGEs were quantified in 58 surface sediment samples across the ponds.

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Article Synopsis
  • Public concern has led to stricter regulations on antimicrobials used in aquaculture, prompting a study of tilapia feed and sediments in southern China.
  • Analysis of 86 compounds revealed that while commercial feeds were mostly tetracycline-free, they still contained other antimicrobials like quinolones and salicylic acid.
  • Sediment samples showed high levels of antibiotics and other residues, indicating that antibiotic-free feed alone may not effectively prevent antibiotic misuse in aquaculture.
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