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Rotational scan digital LAMP for accurate quantitation of nucleic acids.

Lab Chip

June 2021

Materials Science and Engineering, College of Engineering, Peking University, Beijing, China. and Biomedical Pioneering Innovation Center (BIOPIC) and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China and College of Chemistry and Molecular Engineering, and, Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China and Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, China.

Digital quantitation of nucleic acids is precise and sensitive because of its molecular-level resolution. However, only several quantitation formats are common, especially pertaining to how one obtains digital signals from multiple droplets. Here we present rotational scan digital loop-mediated amplification, termed RS-dLAMP.

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