Determination and Prediction of Available Energy in 13 Cereal Feed Ingredients for Growing Pigs.

This study was conducted to develop equations to predict the digestible (DE) and metabolizable energy (ME) for growing pigs by using the chemical compositions of five corn, two wheat and six rice samples. A total of 13 castrated boars were chosen and fed 13 diets formulated with different cereal feed ingredients according to a 13 × 6 Youden square design. The DE and ME contents, the ratio of ME to DE, and the nutrient digestibility among the 13 cereal feed ingredients were different ( < 0.

Antimicrobial Resistance Profiling of Pathogens from Cooked Donkey Meat Products in Beijing Area in One Health Context.

The prevalence of foodborne diseases has raised concerns due to the potential transmission of zoonotic bacterial pathogens through meat products. The objective of this study was to determine the occurrence and antimicrobial resistance (AMR) profiles of pathogenic bacteria in cooked donkey meat products from Beijing. Twenty-one cooked donkey meat samples were collected from different delis, subjected to homogenization, and analyzed for bacterial contamination.

Epidemiology and Molecular Characterization of spp. in Non-Human Primates in Zoos in China.

The genus infects both humans and NHPs. In zoos, visitors feeding significantly increases the frequency of human-to-NHP contact, thereby raising the risk of zoonotic transmission. In this study, six species were investigated and analyzed in the fecal samples of 14 NHP species from zoos in Beijing, Guiyang, Shijiazhuang, Tangshan, and Xingtai in China.

Identification and Characterization of Endophytic Fungus DJE2023 Isolated from Banana ( sp. cv. Dajiao) with Potential for Biocontrol of Banana Fusarium Wilt.

This study characterized an endophytic fungus, DJE2023, isolated from healthy banana sucker of the cultivar (cv.) Dajiao. Its potential as a biocontrol agent against banana Fusarium wilt was assessed, aiming to provide a novel candidate strain for the biological control of the devastating disease.

Comparative Genomics and Pathogenicity Analysis of Three Fungal Isolates Causing Barnyard Grass Blast.

Barnyard grass is one of the most serious rice weeds, often growing near paddy fields and therefore potentially serving as a bridging host for the rice blast fungus. In this study, we isolated three fungal strains from diseased barnyard grass leaves in a rice field. Using a pathogenicity assay, we confirmed that they were capable of causing blast symptoms on barnyard grass and rice leaves to various extents.

Clinical Features, Microbiological Characteristics, and Drug Sensitivity Analysis of Rare Human Spinal Pythiosis Strain.

Pythiosis, a rare and formidable infectious disease caused by , is characterized by profound uncertainties in achieving definitive diagnoses, suboptimal outcomes, and an exceptionally high mortality rate. Here, we present a rare case of human spinal pythiosis in southern China. With advanced metagenomic sequencing technology, was pinpointed as the causative pathogen.

IngredSAM: Open-World Food Ingredient Segmentation via a Single Image Prompt.

Food semantic segmentation is of great significance in the field of computer vision and artificial intelligence, especially in the application of food image analysis. Due to the complexity and variety of food, it is difficult to effectively handle this task using supervised methods. Thus, we introduce IngredSAM, a novel approach for open-world food ingredient semantic segmentation, extending the capabilities of the Segment Anything Model (SAM).

LncRNA Expression Profiles in C6 Ceramide Treatment Reveal lnc_025370 as a Promoter in Canine Mammary Carcinoma CHMp Cells Progression.

Canine mammary carcinomas (CMCs) represent the most prevalent form of cancer in female dogs, characterized by a high incidence and mortality rate. C6 ceramide is recognized for its multifaceted anti-cancer properties, yet its specific influence on CMCs remains to be elucidated. Long noncoding RNAs (lncRNAs), now recognized as functional "dark matter" in precision oncology, are particularly intriguing, with 44% of canine lncRNAs exhibiting tissue-specific expression.

Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label.

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, a method of ultrasensitive immunochromatographic detection of GAT was developed for the first time. An indirect format of the lateral flow immunoassay (LFIA) was performed.

In-House Immunoglobulin Y-Based Immunoassay for Detecting Benzo[a]pyrene in Grilled Pork Samples.

Benzo[a]pyrene (B[a]P) is a hazardous polycyclic aromatic hydrocarbon that accumulates in several environmental matrices as a result of incomplete combustion. Its presence, carcinogenic properties, and tendency for bioaccumulation provide significant risks to human health and the environment. The objective of this study is to create an immunoassay for the detection of benzo[a]pyrene utilizing immunoglobulin Y antibodies.

Generation of transgenic chicken through ovarian injection.

Background: Traditional DNA microinjection methods used in mammals are difficult to apply to avian species due to their unique reproductive characteristics. Genetic manipulation in chickens, particularly involving immature follicles within living ovaries, has not been extensively explored. This study seeks to establish an efficient method for generating transgenic chickens through ovarian injection, potentially bypassing the challenges associated with primordial germ cell (PGC) manipulation and fertilized egg microinjection.

Unlocking the genetic secrets of Dorper sheep: insights into wool shedding and hair follicle development.

Dorper sheep is popular among farming enterprises with strong adaptability, disease resistance, and roughage tolerance, and an unique characteristic of natural shedding of wool. In a large number of observations on experimental sheep farms, it was found that the wool of some sheep still had not shed after May, thus manual shearing was required. Therefore, understanding the molecular mechanisms of normal hair follicles (HFs) development is crucial to revealing the improvement of sheep wool-related traits and mammalian skin-related traits.

Expression Variations of Chloride Ion Channel Related Genes During Diapause Termination in Silkworm, Bombyx mori.

Instant and refrigerated acid soaking are commonly used in cocoon production to prevent or break diapause, and provide developable silkworm eggs for sericulture, while their mechanisms have not been fully understood. This study aims to investigate the mechanisms by which hydrochloric acid (HCl) or dimethyl sulfoxide (DMSO) promotes embryonic development in silkworm Bombyx mori, focusing on the chloride ion (Cl) related gene expression profiles. Our results revealed that the HCl treatment of up to 6 min enhanced hatchability in freshly picked and cold-stored eggs, whereas a slight decrease in hatchability was observed in those treated with DMSO for 40 min.

Modified Vaccinia Virus Ankara Selectively Targets Human Cancer Cells With Low Expression of the Zinc-Finger Antiviral Protein.

Oncolytic viruses are emerging as promising cancer therapeutic agents, with several poxviruses, including vaccinia virus (VACV) and myxoma virus, showing significant potential in preclinical and clinical trials. Modified vaccinia virus Ankara (MVA), a laboratory-derived VACV strain approved by the FDA for mpox and smallpox vaccination, has been shown to be incapable of replicating in human cells unless zinc finger antiviral protein (ZAP) is repressed. Notably, ZAP deficiency is prevalent in various cancer types.

GsMYB10 encoding a MYB-CC transcription factor enhances the tolerance to acidic aluminum stress in soybean.

Background: MYB transcription factors (TFs) play crucial roles in the response to diverse abiotic and biotic stress factors in plants. In this study, the GsMYB10 gene encoding a MYB-CC transcription factor was cloned from wild soybean BW69 line. However, there is less report on the aluminum (Al)-tolerant gene in this subfamily.

Impacts of anthropogenic disturbances on antibiotic resistomes in biological soil crusts on the Qinghai-Tibetan Plateau.

Biological soil crusts (BSCs) are the main landscape on the Qinghai-Tibetan Plateau and an ecological indicator of human disturbance. Information about antibiotic resistomes in BSCs on the Qinghai-Tibetan Plateau can provide baseline for the risk assessment and management of resistomes and yet to be explored. This work investigated the profiles and geographic patterns of antibiotic resistomes in BSCs along the Lhasa River and their response to anthropogenic activities for the first time.

Toxic effects of chlorantraniliprole on zebrafish (Danio rerio) at different developmental stages under antibiotic pressure.

Pesticides and antibiotics have been frequently reported in the environment, but it remains unclear whether antibiotics affect the toxicity of pesticides to aquatic organisms. In this study, the acute, developmental and reproductive toxicity effects of the pesticide chlorantraniliprole on zebrafish at different developmental stages under pressure of ciprofloxacin and erythromycin at environmental concentration were explored. Chlorantraniliprole, ciprofloxacin, and erythromycin are all low toxic to zebrafish (LC > 100 mg/L), and environmental concentrations of antibiotics have no effect on the acute toxicity of chlorantraniliprole to zebrafish.

ZmGolS1 underlies natural variation of raffinose content and salt tolerance in maize.

Salt stress significantly inhibits crop growth and development, and mitigating this can enhance salt tolerance in various crops. Previous studies have shown that regulating saccharide biosynthesis is a key aspect of plant salt tolerance; however, the underlying molecular mechanisms remain largely unexplored. In this study, we demonstrate that overexpression of a salt-inducible galactinol synthase gene, ZmGolS1, alleviates salt-induced growth inhibition, likely by promoting raffinose synthesis.

Genome-wide identification and analysis of autophagy-related (ATG) genes in Lycium ruthenicum Murray reveals their crucial roles in salt stress tolerance.

Autophagy is a highly conserved intracellular degradation system that is crucial for nutrient recycling, thus regulating plant growth and development as well as in response to various stresses. Halophytic plant Lycium ruthenicum Murray (L. ruthenicum) is considered as a potential model plant for studying the physiological mechanisms of salt stress tolerance in plants.

Structural features, physiological functions and digestive properties of phosphorylated corn starch: A comparative study of four phosphorylating agents and two preparation methods.

Phosphorylation is an important modification to modulate functional and digestive properties of starches. We systematically investigated starch phosphorylation process parameters by using two different preparation methods (slurry and semi-dry conditions) and four commonly used phosphorylating agents, namely sodium tripolyphosphate (STPP), sodium trimetaphosphate (STMP), STMP/STPP (99: 1), and sodium phytate (SP). The effects of phosphorylation on physicochemical characteristics, techno-functionalities, digestive properties and structural features of corn starch were analyzed.

Distinct trophic transfer of rare earth elements in adjacent terrestrial and aquatic food webs.

Growing demand and usage of rare earth elements (REEs) lead to significant pollution in wildlife, but trophic transfer of REEs in different food webs has not been well understood. In the present study, bioaccumulation and food web transfer of 16 REEs (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Y, and Sc) were investigated in different terrestrial and aquatic species. Median concentrations of REEs in plant, invertebrate, fish, amphibian, reptile, bird, and vole samples were 488-6030, 296-2320, 123-598, 17.

The SpxA1-TenA toxin-antitoxin system regulates epigenetic variations of Streptococcus pneumoniae by targeting protein synthesis.

Human pathogen Streptococcus pneumoniae forms multiple epigenetically and phenotypically distinct intra-populations by invertase PsrA-driven inversions of DNA methyltransferase hsdS genes in the colony opacity-determinant (cod) locus. As manifested by phase switch between opaque and transparent colonies, different genome methylation patterns or epigenomes confer pathogenesis-associated traits, but it is unknown how the pathogen controls the hsdS inversion orientations. Here, we report our finding of the SpxA1-TenA toxin-antitoxin (TA) system that regulates the orientations of hsdS inversions, and thereby bacterial epigenome and associated traits (e.

Prodrug Self-Assemblies Based on Plant Volatile Aldehydes with Improved Stability and Antimicrobial Activity Against Plant Pathogens.

Plant volatile aldehydes (PVAs) such as cinnamaldehyde (Cin), citral (Cit), citronellal (Citr), and perillaldehyde (Per) have broad-spectrum antimicrobial activity and show great potential in agricultural sustainable production. However, most PVAs not only have very high volatility but also are easily degradable in environment, which seriously restricts their wide application. To address the inherent problems with PVAs, four prodrugs based on PVAs are fabricated by conjugating individually Cin, Cit, Citr, and Per to sodium bisulfite (Sod) through a simple addition reaction and subsequently self-assembled into nanoparticles (prodrug self-assemblies) in aqueous solutions.

Effect of pesticide application techniques on thrips control in the cowpea flowering stage.

Background: Thrips are among the most damaging pests to cowpeas in Hainan, China. Conventional pesticide application methods often fail to achieve satisfactory control due to the small size and concealed habitats of thrips. This study aimed to enhance the efficacy of pesticides by improving their application techniques.