Ovine Mined from RNA-Seq: Identification, Expression Profile, Promotion of Preadipocyte Differentiation, Promoter Activity, and Its Polymorphisms Related to Phenotypic Traits.

Tail fat is essential for sheep survival in extreme environments, yet its significance is often overlooked, leading to the decline of fat-tailed breeds. This study identified a novel lncRNA, (), through transcriptome sequencing, showing differential expression in the tail adipose tissues of Lanzhou Fat-Tailed (LFT) sheep and Tibetan (TS) sheep. Highly expressed in adipose tissues, inhibits preadipocyte proliferation and promotes 3T3-L1 differentiation, suggesting its role in regulating fat deposition.

Prevalence of antibiotic resistance genes in mining-impacted farmland environments.

Mining activities produce large quantities of tailings and acid mine drainage, which contain varieties of heavy metals, thereby affecting the downstream farmland soils and crops. Heavy metals could induce antibiotic resistance through co-selection pressure. However, the profiles of antibiotic resistance genes (ARGs) in the mining-affected farmland soils and crops are still unclear.

Exploring the mechanisms by which common inhalational anesthetics influence malignant tumor metastasis: A data mining study based on comparative toxicogenomic databases.

Surgery remains the primary treatment for solid malignant tumors, but controlling postoperative tumor recurrence and metastasis continues to be a major challenge. Understanding the factors that influence tumor recurrence and metastasis after surgery, as well as the underlying biological mechanisms, is critical. Previous studies suggest that anesthetic agents may increase the risk of tumor recurrence and metastasis in patients with cancer, but the mechanisms underlying these findings remain unclear.

Hedyotis diffusa injection modulates the ferroptosis in bladder cancer via CAV1/JUN/VEGFA.

Hedyotis diffusa Willd. (HDW), a traditional Chinese medicinal plant, exhibits a variety of pharmacological effects and has anticancer potential for a wide range of cancer types; Ferroptosis is a non-apoptosis-regulated cell death induced by iron accumulation and subsequent lipid peroxidation; and there is currently an increasing interest in the therapeutic role of ferroptosis in cancer. However, the effects of HDW on bladder cancer and its underlying molecular mechanisms remain largely unknown.

Integration of gene expression and DNA methylation data using MLA-GNN for liver cancer biomarker mining.

The early symptoms of hepatocellular carcinoma patients are often subtle and easily overlooked. By the time patients exhibit noticeable symptoms, the disease has typically progressed to middle or late stages, missing optimal treatment opportunities. Therefore, discovering biomarkers is essential for elucidating their functions for the early diagnosis and prevention.

Downregulation of SLC7A11 by Bis(4-Hydroxy-3,5-Dimethylphenyl) Sulfone Induces Ferroptosis in Hepatocellular Carcinoma Cell.

The progression of tumors has been demonstrated to have a strong correlation with ferroptosis. Bis(4-hydroxy-3,5-dimethylphenyl) sulfone (TMBPS) has been shown to effectively inhibit the proliferation of hepatocellular carcinoma (HCC), but its underlying mechanism is not clear. In this study, ferrostatin-1 (Fer-1) was employed to explore whether the death of HCC cells caused by TMBPS is related to ferroptosis.

Quality, topics, and demographic trends of animal systematic reviews - an umbrella review.

Background: Animal systematic reviews are critical to inform translational research. Despite their growing popularity, there is a notable lack of information on their quality, scope, and geographical distribution over time. Addressing this gap is important to maintain their effectiveness in fostering medical advancements.

Research on the acoustic emission characteristics and instability correlation effect of the dynamic response of waste dump slopes.

Due to the fluidity of the loose medium inside the waste dump slope, the traditional monitoring system cannot fully reflect the misalignment and slip between particles inside the medium, and it is also difficult to capture the precursor information of the slip of the loose accumulation body. To reveal the dynamic evolution process of the slope instability of the waste dump slope, the coupling test system of the slope instability of the waste dump slope was used to carry out the study of the acoustic emission characteristics of the slope instability dynamic response of the dump slope under the action of vibration, and to quantitatively analyse the staged characteristics of the acoustic emission parameter evolution of the dump slope under the action of different vibration frequencies and its instability initiation node. The results show that with the increase of vibration frequency, the damage mode of the slope model gradually changes from sliding of small particles to large-scale landslides, and presents the stage process of "vibration compaction → vibration equilibrium → dynamic instability"; Under the action of low-frequency and high-amplitude, the slope model mainly shows that the tiny particles and the basement gravel slip, which is difficult to capture with the naked eye, while under the action of high-frequency and low-amplitude, the slope surface is damaged in a large area, and the overall model is unstable; The dynamic instability of the waste dump slope is accompanied by obvious acoustic emission activities, and the changes of the characteristic parameters of acoustic emission reveal, to a certain extent, the evolution of the internal state of the slope in the process of dynamic instability of the waste dump slope and its stage characteristics; The amplitude and energy efficiency of acoustic emission in the time domain show obvious fractal characteristics in the dynamic instability of the waste dump slope.

Surrounding rock control for the roadway driven under the goafs of small collieries and heading for adjacent advancing face.

Aiming at the problems of complex stress and large deformations in the surrounding rocks of the roadway driven under the goafs of small collieries and heading for adjacent advancing coal face, by numerical modeling and field practice, the failure characteristics of the overlying coal and rocks were investigated, and the stopping and resuming times of the roadway excavation were identified. A zoning-based reinforcement technique was put forward and applied in engineering practice. The results showed that (1) The roadway roof was divided into four zones: "Rolid coal zone", "Residual pillar zone", "Roof caved zone", and "Roof un-caved zone".

Tissue-specific expression, functional analysis, and polymorphism of the KRT2 gene in sheep horn.

Horn is a defensive weapon of sheep, consisting of a horny sheath and a bony core. The KRT2 gene is related to keratinization of the epidermis, so it is likely to be one of the contributor genes affecting horn type in sheep. In this study, we first analyzed the species-specific and tissue-specific expression of the KRT2 gene using transcriptome sequencing data.

Mapping the current trends and hotspots of extracellular vesicles in Alzheimer's disease: a bibliometric analysis.

Background: Extracellular vesicles (EVs) have garnered significant attention in Alzheimer's disease (AD) research over the past decade, largely due to their potential in diagnostics and therapeutics. Although the investigation of EVs in AD is a relatively recent endeavor, a comprehensive bibliometric analysis of this rapidly growing field has yet to be conducted.

Methods: This study aims to elucidate and synthesize the relationship between EVs and AD, offering critical insights to guide future research and expand therapeutic possibilities.

An optimized public opinion communication system in social media networks based on K-means cluster analysis.

This study proposes a public opinion monitoring model that combines the K-means clustering algorithm with Particle Swarm Optimization (PSO) to enhance the accuracy and effectiveness of public opinion monitoring on social media. The model's performance across various dissemination indicators is studied in detail. Through experiments conducted on social media datasets, the study comprehensively evaluates the model from four dimensions: dissemination speed, scope, depth, and sentiment dissemination effectiveness.

Physical Simulation Experiment on the Rock Breaking Efficiency of Pulse Type Controllable Shock Wave.

Given that conducting controllable shock wave tests in actual rock formations underground in coal mines affects coal mine production with the parameters required for equipment design and incurs significant costs, a series of ground tests were conducted separately. First, the impact of energy storage on rock breaking efficiency was analyzed. Then, physical simulation experiments were conducted on the differential efficiency of controllable shock waves on high-strength cement, sandstone, granite, solid granite, and limestone.

Synthesis and Characterization of NaX Zeolite from Coal Gangue and Its Efficacy in Cd and Pb Remediation in Water and Soil.

Alkaline fusion is a pivotal process influencing the cost of synthesizing zeolite from coal gangue. This study examined the effects of alkaline fusion temperature ( ), treatment duration ( ) and the NaOH/coal gangue weight ratio ( ) on the composition and properties of the products, as well as their adsorption capacities for Cd ( ) and Pb ( ). Response surface methodology (RSM) was employed to analyze the interactions among these factors, and the adsorption mechanisms for Cd and Pb were investigated using X-ray diffraction, scanning electron microscopy-EDS, Fourier transform infrared, X-ray photoelectron spectroscopy, and N adsorption-desorption techniques.

Bibliometric Analysis and Visualization of Nanoindentation Research.

Nanoindentation has gained significant attention as a method for the quantitative characterization of mechanical properties of materials at micro- and nanoscales. This study investigates trends in nanoindentation technology by visualizing and analyzing relevant publications. To achieve this, CiteSpace was utilized to analyze 14 373 papers from the Web of Science core database published between 2013 and 2022.

Multiscale Structural Control by Matrix Engineering for Polydimethylsiloxane Filled Graphene Woven Fabric Strain Sensors.

Elastomer cure shrinkage during composite fabrication often induces wrinkling in conductive networks, significantly affecting the performance of flexible strain sensors, yet the specific roles of such wrinkles are not fully understood. Herein, a highly sensitive polydimethylsiloxane-filled graphene woven fabric (PDMS-f-GWF) strain sensor by optimizing the PDMS cure shrinkage through careful adjustment of the base-to-curing-agent ratio is developed. This sensor achieves a gauge factor of ∼700 at 25% strain, which is over 6 times higher than sensors using commercially formulated PDMS.

Microwave-Sintered Nano-SiC Reinforced 8SiC/Ti-3Cu Composite: Fabrication, Wear Resistance, Antibacterial Function, and Biocompatibility.

The significance of biomedical applications of Ti alloys is best emphasized by their widespread utilization as implantable materials, such as internal supports and bone replacements. Ti alloys are sensitive to fretting wear, which leads to the early failure of Ti implants. Improved wear resistance of such implants is essential to ensure a prolonged implant life.

The synthesis of an iron tailings-based geopolymer with synergistic electromagnetic wave consumption property.

In this study, combination of wave absorption materials with different loss mechanisms are added into iron ore tailings-blast furnace slag (IOT-BFS) based geopolymers. The employed materials are hollow glass microsphere (HGM), carbon nanotubes (CNT) and carbonyl iron powder (CIP). Microstructures of the geopolymers are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and concrete porous structure analyzer.

SERS-based simplified analysis of paraquat in poisoning cases: Bypassing complicated pretreatment with antioxidant sensor.

Applying antioxidant coating materials to prepare surface-enhanced Raman spectroscopy (SERS) sensing substrates can effectively enhance the sensitivity and stability for the analysis of molecules. In this study, we have leveraged SERS to develop an innovative sensor for the swift identification of Paraquat (PQ), enabling on-site detection of this herbicide. The newly devised sensor distinguishes itself through its exceptional oxidation resistance.

Groundwater arsenic and antimony mobility from an antimony mining area: Controls of sulfide oxidation, carbonate and silicate weathering, and secondary mineral precipitation.

Sulfide mineral oxidation has been recognized as the key driver of arsenic (As) and antimony (Sb) mobility in mining-impacted groundwater. However, the role of carbonate and silicate weathering and secondary mineral precipitation in this process remain unknown. A comprehensive geochemical study of groundwater was conducted in an Sb-mining area, Hunan, China, with samples collected from aquifers of the Xikuangshan Formation (Dx), the Shetianqiao Formation (Ds ), and the Lower Carboniferous Formation (Cy).

Association of dietary live microbe intake with all-cause and cardiovascular mortality in an older population: Evidence from NHANES 2003-2018.

Background: The relationship between dietary live microbe intake, non-dietary prebiotics/probiotics, and mortality in older adults remains unclear.

Methods: Participants from the National Health and Nutrition Examination Survey 2003-2018 were included. Participants were categorized into three groups based on estimated live microbe intake: low, medium, and high.

Preliminary study on the explosive performance of coal dust and prospects for engineering applications.

This study aims to evaluate the efficiency and energy release characteristics of different types of coal in pulse detonation engines (PDE) to advance the development of deep coal fluidization detonation technology, achieving more efficient and cleaner coal utilization. Using a custom PDE setup, experiments were conducted with four coal types at mass flow rates from 30 to 120 g/s. High-frequency pressure sensors assessed pressure dynamics and detonation wave propagation, complemented by numerical simulations for accuracy.

Design Principles for Gradient Porous Carbon on Aqueous Zinc-Ion Hybrid Capacitors: A Combined Molecular Dynamic and Machine Learning Study.

Gradient porous carbon has become a potential electrode material for energy storage devices, including the aqueous zinc-ion hybrid capacitor (ZIHC). Compared with the sufficient studies on the fabrication of ZIHCs with high electrochemical performance, there is still lack of in-depth understanding of the underlying mechanisms of gradient porous structure for energy storage, especially the synergistic effect of ultramicropores (<1 nm) and micropores (1-2 nm). Here, we report a design principle for the gradient porous carbon structure used for ZIHC based on the data-mining machine learning (ML) method.

Genome-wide development of simple sequence repeat (SSR) markers at 2-Mb intervals in lotus (Nelumbo Adans.).

Background: Despite the rapid advancement of high-throughput sequencing, simple sequence repeats (SSRs) remain indispensable molecular markers for various applied and research tasks owing to their cost-effectiveness and ease of use. However, existing SSR markers cannot meet the growing demand for research on lotus (Nelumbo Adans.) given their scarcity and weak connections to the lotus genome.