1 results match your criteria: "China Shanghai Research and Development Center of Industrial Biotechnology[Affiliation]"
High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage.
Appl Environ Microbiol
July 2014
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China Shanghai Research and Development Center of Industrial Biotechnology, Shanghai, China
Genetic modifications of bacterial chromosomes are important for both fundamental and applied research. In this study, we developed an efficient, easy-to-use system for genetic modification of the Escherichia coli chromosome, a two-plasmid method involving lambda Red (λ-Red) recombination and I-SceI cleavage. An intermediate strain is generated by integration of a resistance marker gene(s) and I-SceI recognition sites in or near the target gene locus, using λ-Red PCR targeting.
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