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[Study on proteins in Guangdilong by nano LC/orbitrap fusion lumos HR-MS].
Zhongguo Zhong Yao Za Zhi
January 2019
Shanghai Institute for Food and Drug Control Shanghai 201203, China Shanghai Institute of Pharmaceutical Industry Shanghai 201203,China.
In the present study,fresh Guangdilong( GD),originating from Pheretima aspergillum,was taken as the object. The total proteins from GD were firstly separated by SDS-PAGE according to their molecular weights and in-gel digestion was then performed.After that,the peptides were analyzed by nano LC/orbitrap fusion lumos high resolution mass spectrometry( nano LC/orbitrap fusion lumos HR-MS).
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