An enzyme-activated loop primer probe LAMP method based on a new SNP site in the group_17537 gene for rapid on-site detection of Salmonella Pullorum.

Pullorum disease (PD) is a widespread disease that causes significant economic losses within the poultry industry of developing countries. An effective strategy for its prevention and control involves the implementation of decontamination procedures utilizing highly specific on-site detection techniques. In this study, a single-nucleotide polymorphism (SNP) site within the group_17537 gene of Salmonella enterica serovar Gallinarum biovars Pullorum (S.

Discovery of novel dual tubulin and MMPs inhibitors for the treatment of lung cancer and overcoming drug resistance.

Nowadays, hybrid molecule with dual targets activity or effect is regarded as an effective strategy for combating the drug resistance development in cancer therapy. Herein, novel of bifunctional conjugates targeting tubulin and MMPs inhibitors were synthesized. Among them, 15j exhibited robust anticancer activity in vitro and in vivo, with IC values of 0.

ACC treatment induced alterations in flavonoid accumulation in Toxicodendron vernicifluum.

Lacquer tree (Toxicodendron vernicifluum) is an important economic crop and is rich in flavonoids. ACC (1-aminocyclopropane-1-carboxylic acid) is the precursor to ethylene. ACC treatment can induce physiological and biochemical responses in plants.

Exploring the experience of stigma among children and adolescents with epilepsy in China: A qualitative study.

Objective: Epilepsy-related stigma negatively affects the lives of children and adolescents. This study aims to explore the experience of stigma among children and adolescents with epilepsy in China.

Methods: This study used purposive sampling to select children and adolescents aged 10-18 diagnosed with epilepsy.

Coptisine improves LPS-induced anxiety-like behaviors by regulating the Warburg effect in microglia via PKM2.

Neuroinflammation mediated by microglia is considered the primary cause and pathological process of anxiety. Abnormal glycolysis of microglia is observed during microglia activation. However, whether regulating the Warburg effect in microglia can effectively intervene anxiety and its potential mechanisms have not been elucidated.

To add or to remove? The role of working memory updating in preschool children's non-symbolic arithmetic abilities between addition and subtraction.

Early computational capacity sets the foundation for mathematical learning. Preschool children have been shown to perform both non-symbolic addition and subtraction problems. However, it is still unknown how different operations affect the representational precision of the non-symbolic arithmetic solutions.

Identification of the primary pollution sources and dominant influencing factors of soil heavy metals using a random forest model optimized by genetic algorithm coupled with geodetector.

Identifying and quantifying the dominant factors influencing heavy metal (HM) pollution sources are essential for maintaining soil ecological health and implementing effective pollution control measures. This study analyzed soil HM samples from 53 different land use types in Jiaozuo City, Henan Province, China. Pollution sources were identified using Absolute Principal Component Score (APCS), with 8 anthropogenic factors, 9 natural factors, and 4 soil physicochemical properties mapped using Geographic Information System (GIS) kernel density estimation.

Inducers of Autophagy and Cell Death: Focus on Copper Metabolism.

Copper is an essential trace element in biological systems, playing a key role in various physiological functions, including redox reactions and energy metabolism. However, an imbalance in copper homeostasis can induce oxidative stress, mitochondrial dysfunction, and inhibition of the ubiquitin-proteasome system, ultimately leading to significant cytotoxicity and cell death. According to recent research, copper can bind to lipoylation sites on proteins involved in the tricarboxylic acid cycle, causing aggregation of lipoylated proteins, the loss of Fe-S cluster proteins, proteotoxic stress, and ultimately, cell death.

Case-Based Virtual Reality Simulation for Severe Pelvic Trauma Clinical Skill Training in Medical Students: Design and Pilot Study.

Background: Teaching severe pelvic trauma poses a significant challenge in orthopedic surgery education due to the necessity of both clinical reasoning and procedural operational skills for mastery. Traditional methods of instruction, including theoretical teaching and mannequin practice, face limitations due to the complexity, the unpredictability of treatment scenarios, the scarcity of typical cases, and the abstract nature of traditional teaching, all of which impede students' knowledge acquisition.

Objective: This study aims to introduce a novel experimental teaching methodology for severe pelvic trauma, integrating virtual reality (VR) technology as a potent adjunct to existing teaching practices.

Emulsion Polymerization of Styrene to Polystyrene Nanoparticles with Self-Emulsifying Nanodroplets as Nucleus.

The mechanism of the emulsion polymerization of styrene to polystyrene nanoparticles (PSNPs) remains a subject of debate. Herein, a series of reaction parameters with different surfactant concentrations, monomer contents, temperatures, and equilibration times were investigated to understand the formation mechanism of PSNPs, which demonstrate a correlation between the properties of PSNPs and the mesostructure of the premix. Cooling the model systems with self-emulsifying nanodroplets (SENDs) in the early reaction stages resulted in the hollow polystyrene spheres (H-PSSs), ruptured PSNPs, and dandelion-like PSNPs, further indicating that the oil nanodroplets are the key sites for the formation of PSNPs.

Unveiling the Werner-Type Cluster Chemistry of Heterometallic 4f/Post-Transition Metals: A {DyBi} Complex Exhibiting Quantum Tunneling Steps in the Hysteresis Loops and its 1-D Congener.

A new [DyBiOCl(saph)] () Werner-type cluster has been prepared, which is the first Dy/Bi polynuclear compound with no metal-metal bond and one of the very few Ln-Bi (Ln = lanthanide) heterometallic complexes reported to date. The molecular compound has been deliberately transformed to its 1-D analogue [DyBiO(N)(saph)] () via the replacement of the terminal Cl ions by end-to-end bridging N groups. The overall metallic skeleton of (and ) can be described as consisting of a diamagnetic {Bi} unit with an elongated trigonal bipyramidal topology, surrounded by a magnetic {Dy} equilateral triangle, which does not contain μ-oxo/hydroxo/alkoxo groups.

Development and laboratory evaluation of a novel IoT-based electric-driven metering system for high precision garlic planter.

In order to address many issues, such as the inconsistent and unreliable seeding process in traditional mechanical garlic seed metering systems (SMS), as well as the lack of ability to monitor the effectiveness of the seeding, a highly accurate electric-driven metering system (EDMS) was developed and created specifically for garlic seed planters. This study provided a description of the overall structure and functioning principle, as well as an analysis of the mechanism for smooth transit and delivery. A combination of an infrared (IR) sensor, Arduino Mega board, stepper motor, speed sensor, and a Wi-Fi module was employed to operate the EDMS, as well as monitor and count the quantity of garlic seeds during the planting process and determine the qualified rate (QR) and missing rate (MR).

Composition and functional diversity of soil and water microbial communities in the rice-crab symbiosis system.

Rice-crab co-culture is an environmentally friendly agricultural and aquaculture technology with high economic and ecological value. In order to clarify the structure and function of soil and water microbial communities in the rice-crab symbiosis system, the standard rice-crab field with a ring groove was used as the research object. High-throughput sequencing was performed with rice field water samples to analyze the species and abundance differences of soil bacteria and fungi.

A Review of Laboratory Studies on the Heterogeneous Chemistry of NO: Mechanisms and Uptake Kinetics.

NO is a significant primary atmospheric pollutant that plays a key role in atmospheric chemistry. It serves as a crucial precursor to photochemical smog, acid rain, and secondary particulate matter and is instrumental in determining the atmospheric oxidation capacity. In this review, we focus on the heterogeneous chemistry of NO, which has been demonstrated to significantly influence the sources and sinks of various nitrogen-containing species through field measurements and model simulations.

Translation, adaptation and validation of an epilepsy screening instrument in two Ghanaian languages.

Introduction: The prevalence of epilepsy in sub-Saharan Africa varies considerably, and the exact estimate for Ghana remains unclear, particularly in peri-urban areas where data are scarce. More community-based studies are required to understand better the actual burden of epilepsy in these areas and the difficulties in accessing healthcare.

Objective: To adapt and validate a household survey epilepsy-screening instrument in Shai-Osudoku and Ningo-Prampram District of Greater Accra Region, Ghana.

Effect of family support and work resources in the relationship of economic constraints and work volition: Evidence from China.

Background: As the pace of economic development slows, college students are facing an increasingly challenging employment landscape. For instance, the expansion of higher education has led to a swell in the number of job seekers, which has in turn intensified competition. Given the limited job opportunities, it's understandable that many college students are developing a pessimistic employment mindset.

The artificial intelligence-based agricultural field irrigation warning system using GA-BP neural network under smart agriculture.

This work explores an intelligent field irrigation warning system based on the Enhanced Genetic Algorithm-Backpropagation Neural Network (EGA-BPNN) model in the context of smart agriculture. To achieve this, irrigation flow prediction in agricultural fields is chosen as the research topic. Firstly, the BPNN principles are studied, revealing issues such as sensitivity to initial values, susceptibility to local optima, and sample dependency.

The transcription factor GmFULc regulates soybean plant height by binding the promoter of a gibberellin-responsive gene.

Plant height is a crucial agronomic characteristic that substantially influences soybean [Glycine max (L.) Merr.] yield.

A Dual-Channel Fluorescence Probe for Early Diagnosis and Treatment Monitoring of Acute Kidney Injury by Detecting HOCl and Cys with Different Fluorescence Signals.

The pathogenesis of acute kidney injury (AKI) is a multifaceted process involving various mechanisms, with oxidative stress playing a crucial role in its development. Hypochlorite (HOCl) and cysteine (Cys) are indicators of oxidative stress in AKI pathophysiology, directly reflecting the degree of oxidative stress and disease severity. However, their exact mechanism of action in AKI pathophysiology remains unknown.

Distribution and Environmental Preference of Potential Mercury Methylators in Paddy Soils across China.

The neurotoxin methylmercury (MeHg) is produced mainly from the transformation of inorganic Hg by microorganisms carrying the gene pair. Paddy soils are known to harbor diverse microbial communities exhibiting varying abilities in methylating inorganic Hg, but their distribution and environmental drivers remain unknown at a large spatial scale. Using gene amplicon sequencing, this study examined Hg-methylating communities from major rice-producing paddy soils across a transect of ∼3600 km and an altitude of ∼1300 m in China.

Rapid radiation of a plant lineage sheds light on the assembly of dry valley biomes.

Southwest China is characterized by high plateaus, large mountain systems, and deeply incised dry valleys formed by major rivers and their tributaries. Despite the considerable attention given to alpine plant radiations in this region, the timing and mode of diversification of the numerous dry valley plant lineages remain unknown. To address this knowledge gap, we investigated the macroevolution of Isodon (Lamiaceae), a lineage commonly distributed in the dry valleys in southwest China and wetter areas of Asia and Africa.

ERF114/115/109 are essential for jasmonate-repressed non-canonical JAZ8 activity in JA signaling.

Jasmonate (JA), a key plant hormone, regulates various aspects of plant development and stress responses, primarily through the degradation of canonical jasmonate-ZIM domain (JAZ) proteins by the SCF complex. While JAZ8, a non-canonical JAZ protein lacking the degron signal, has been shown to repress JA responses, the mechanism by which JA inhibits JAZ8 activity remains unclear. Here, we demonstrate that Arabidopsis ethylene response factor 114 (ERF114), ERF115, and ERF109 regulate JA signaling through interacting with JAZ8.

Seasonal Fluctuation in the Incidence of Congenital Hypothyroidism Across Different Temperate Zones of China.

Background: Seasonality in the incidence of congenital hypothyroidism (CH) has been identified in several countries and different conclusions have been drawn. The objective of this study was to examine whether this seasonality is also observable in China and how it manifests across different temperate zones.

Methods: Data on CH cases and screened neonates between January 1, 2014, and September 30, 2022, by year and season, were sourced from the Chinese Newborn Screening Information System.

Cloning and functional verification of gene in .

Background: Geraniol 10-hydroxylase (G10H) is a cytochrome P450 monooxygenase involved in regulation, which is involved in the biosynthesis of monoterpene. However, G10H is not characterized at the enzymatic mechanism and regulatory function in .

Methods And Results: A gene related to the biosynthesis of monoterpenoid, geraniol 10-hydroxylase, has been cloned from the medicinal plant .