Complete genome sequence of Bacillus amyloliquefaciens B15 isolated from grape skin, a strain of strong inhibitory activity against fungi.

Bacillus amyloliquefaciens B15 is a Gram-positive, plant-associated bacterium which shows strong antifungal activity, isolated from grape skin in Xinjiang, China. The genome of B. amyloliquefaciens B15 comprises a 4,006,754bp long circular chromosome containing 3991 protein coding genes and 109 RNA genes.

Fructose and glucose combined with free fatty acids induce metabolic disorders in HepG2 cell: A new model to study the impacts of high-fructose/sucrose and high-fat diets in vitro.

Scope: This work investigated the underlying mechanism of high-fructose/sucrose and high-fat diets, which rapidly induce metabolic syndrome in vivo, via a new cell model.

Methods And Results: Glucose and/or fructose were used to induce the human hepatoma cell (HepG2) in the presence of palmitic acid, oleic acid, or combined fatty acids (CFA) for 24 h. The alterations in lipid and uric acid production, glucose metabolism, oxidative status, and related genes and proteins were monitored.

Paenibacillus chinensis sp. nov., isolated from maize (Zea mays L.) seeds.

Four Gram-stain positive bacterial strains, designated as 4R1(T), 4R9, 4L13 and 4L18, isolated from seeds of hybrid maize (Zea mays L., Jingke 968), were investigated using a polyphasic taxonomic approach. The cells were found to be facultatively aerobic, motile, spore-forming and rod-shaped.

Characterization, Genome Sequence, and Analysis of Escherichia Phage CICC 80001, a Bacteriophage Infecting an Efficient L-Aspartic Acid Producing Escherichia coli.

Escherichia phage CICC 80001 was isolated from the bacteriophage contaminated medium of an Escherichia coli strain HY-05C (CICC 11022S) which could produce L-aspartic acid. The phage had a head diameter of 45-50 nm and a tail of about 10 nm. The one-step growth curve showed a latent period of 10 min and a rise period of about 20 min.

Paenibacillus zeae sp. nov., isolated from maize (Zea mays L.) seeds.

Four Gram-stain-positive bacterial strains, designated 6R2T, 6R18, 3T2 and 3T10, isolated from seeds of hybrid maize (Zea mays L., Jingke 968) were investigated using a polyphasic taxonomic approach. Cells were aerobic, motile, spore-forming and rod-shaped.

Rapid Method for the Determination of the Stable Oxygen Isotope Ratio of Water in Alcoholic Beverages.

This paper demonstrates the first successful application of an online pyrolysis technique for the direct determination of oxygen isotope ratios (δ(18)O) of water in alcoholic beverages. Similar water concentrations in each sample were achieved by adjustment with absolute ethyl alcohol, and then a fixed GC split ratio can be used. All of the organic ingredients were successfully separated from the analyte on a CP-PoraBond Q column and subsequently vented out, whereas water molecules were transferred into the reaction furnace and converted to CO.

Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines.

Background: Glycoproteins play a critical role in the cellular activities of eukaryotes. Sialic acid is typically the outermost monosaccharide of glycolipids and glycoproteins, and is necessary for normal development.

Results: A strategy based on avidin-biotin affinity was established to enrich sialylated glycoproteins from HeLa cervical carcinoma, SW1990 pancreatic adenocarcinoma, and A549 lung adenocarcinoma cells.

Synergistic interactions of apigenin, naringin, quercetin and emodin on inhibition of 3T3-L1 preadipocyte differentiation and pancreas lipase activity.

The interactions of four natural compounds including apigenin, naringin, emodin and quercetin were investigated on inhibiting 3T3-L1 preadipocyte differentiation and pancreas lipase activity. Oil Red O staining was conducted to visualise and quantify lipid accumulation. The difference between experimental and calculated results was utilised for determining the interaction types.

Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch.

A Gram-stain-positive bacterial strain, designated as NR2T, isolated from noni (Morinda citrifolia L.) branch was investigated using a polyphasic taxonomic approach. The cells were small coccoid to ovoid, non-spore-forming and motile.

Enzymatic synthesis of rare sugars with L-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8.

L-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars D-psicose, D-sorbose, L-tagatose and L-fructose using our one-pot four-enzyme system.

Aflatoxin B1 and aflatoxin M1 induced cytotoxicity and DNA damage in differentiated and undifferentiated Caco-2 cells.

Aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) are natural mycotoxins that frequently present in food and feed and pose risks to human health. There are few data in the literature regarding the impairment of them in the intestine. Therefore, the present study investigated their cytotoxic effect on Caco-2 cells, especially the differentiated ones that resemble mature small intestinal enterocytes.

Sphingomonas morindae sp. nov., isolated from Noni (Morinda citrifolia L.) branch.

Two yellow bacterial strains, designated NBD5(T) and NBD8, isolated from Noni (Morinda citrifolia L.) branch were investigated using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-spore-forming, non-motile and short rod-shaped.

The chemistry of sour taste and the strategy to reduce the sour taste of beer.

The contributions of free hydrogen ions, undissociated hydrogen ions in protonated acid species, and anionic acid species to sour taste were studied through sensory experiments. According to tasting results, it can be inferred that the basic substance producing a sour taste is the hydrogen ion, including free hydrogen ions and undissociated hydrogen ions. The intensity of a sour taste is determined by the total concentration of free hydrogen ions and undissociated hydrogen ions.

Bacteriophage therapy against Enterobacteriaceae.

The Enterobacteriaceae are a class of gram-negative facultative anaerobic rods, which can cause a variety of diseases, such as bacteremia, septic arthritis, endocarditis, osteomyelitis, lower respiratory tract infections, skin and soft-tissue infections, urinary tract infections, intra-abdominal infections and ophthalmic infections, in humans, poultry, animals and fish. Disease caused by Enterobacteriaceae cause the deaths of millions of people every year, resulting in enormous economic loss. Drug treatment is a useful and efficient way to control Enterobacteriaceae infections.

Genome Sequence of Thermoactinomyces daqus H-18, a Novel Thermophilic Species Isolated from High-Temperature Daqu.

Thermoactinomyces daqus H-18 is a new species of Thermoactinomyces isolated from high-temperature Daqu used in the fermentation of Bandongjing sesame-flavor liquor. Its genome was sequenced and assembled (3.44 Mb).

Pilot-scale production of soybean oligopeptides and antioxidant and antihypertensive effects in vitro and in vivo.

Soybean oligopeptides (SOP) with low molecular weights were prepared by two-step enzymatic hydrolysis on a pilot-scale. Peptide and free amino acid contents of SOP were 82.5 ± 1.

Genome Sequence of Luteimonas huabeiensis HB-2, a Novel Species of Luteimonas with High Oil Displacement Efficiency.

Luteimonas huabeiensis HB-2 is a novel and newly isolated strain, which shows a superior property of oil displacement. Here, we present a 4.3-Mb assembly of its genome.

Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature Daqu.

Daqu is a fermentation starter used in the production of Chinese liquors. A thermophilic bacterium, designated strain H-18(T), was isolated from a high-temperature Daqu sample collected from the manufacturing process of a sesame-flavoured liquor in Shandong province, China. It was investigated in a taxonomic study using a polyphasic approach.

Luteimonas huabeiensis sp. nov., isolated from stratum water.

A yellow-coloured bacterial strain, designated HB2(T), isolated from stratum water was investigated using a polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was a member of the genus Luteimonas, its three closest neighbours being Luteimonas aquatica BCRC 17731(T) (97.

Isolation and identification of antioxidative peptides from pilot-scale black-bone silky fowl (Gallus gallus domesticus Brisson) muscle oligopeptides.

Background: A pilot-scale production was developed to produce oligopeptide powder from black-bone silky fowl (Gallus gallus domesticus Brisson) muscle (BSFP) by two-step enzymatic hydrolysis and multistage separation. The resultant BSFP was assessed for antioxidant activities against four free radicals (hydroxyl, 1,1-dipheny-2-picrylhydrazyl (DPPH), superoxide and peroxyl) and against the peroxidation of linoleic acid in a lipid peroxidation model system. After separation by reversed-phase high-performance liquid chromatography (RP-HPLC), five major fractions of BSFP were tested for DPPH radical scavenging activity and subjected to mass spectrometry to identify the active peptides.

[Advances of solid-phase microextraction and current status of application in food analysis].

Solid-phase microextraction (SPME) has been widely used as a sample preparation technique in current chromatography. SPME integrates extraction, concentration, desorption and injection in one step, which is highly sensitive and easy to operate. This review briefly introduces the evolution of the coating, equipment and corresponding techniques of SPME in recent years.

[The phylogenetic analysis of 15 Geotrichum strains based on 26S rRNA gene D1/D2 region sequencing].

The 26S rRNA gene D1/D2 domain sequences of 15 strains originally identified as Galactomyces geotrichum from the Chinese Industry Culture Collection (CICC) were determined. The results indicated that these strains differed from the type strain of Galactomyces geotrichum and other species of the genus remarkably. Two groups were recognized from the 15 strains which possibly represent 2 novel species of Galactomyces.

[Determination of major flavor components in chinese spirits using a special domestic gas chromatograph with DNP packed column].

A method using a domestic gas chromatograph controlled by computer with a homemade DNP packed column for the determination of major flavor components in Chinese spirtis is described. This chromatograph was developed under the sponsorship of the Ministry of Science and Technology. Optimizations of the operating parameters were undertaken in order to establish the chromatographic conditions suitable for isothermal and programmed temperature gas chromatography.

[Determination of minor flavor components in Chinese spirits by direct-injection technique with capillary columns].

Chinese spirits are among the best-known distilled alcoholic beverages in the world. Because of the longer multiple-strain fermentation, Chinese spirits are richer than yeast fermented western liquors in fragrance and mellow. The complex nature of Chinese spirits often makes it difficult to separate, identify and quantify major and minor flavor components.