KPNB1-ATF4 induces BNIP3-dependent mitophagy to drive odontoblastic differentiation in dental pulp stem cells.

Background: Differentiating dental pulp stem cells (DPSCs) into odontoblasts is a critical process for tooth self-repair and dentine‒pulp engineering strategies in the clinic. However, the mechanism underlying the regulation of DPSC odontoblastic differentiation remains largely unknown. Here, we demonstrated that BCL-2 interacting protein 3 (BNIP3)-dependent mitophagy is associated with importin subunit beta-1 (KPNB1)-activating transcription factor 4 (ATF4), which promotes DPSC odontoblastic differentiation.

[Transverse characteristics of normodivergent patients in different sagittal skeletal patterns].

Purpose: This study aimed to measure and analyze the transverse indicators of normodivergent patients with different sagittal skeletal malocclusions, to explore the transverse characteristics of different sagittal skeletal malocclusions.

Methods: Lateral cephalograms and CBCT of 90 normodivergent patients with skeletal Class Ⅰ, Ⅱ and Ⅲ in their permanent dentition were collected. Dolphin software was applied to measure the widths of the basal bone, alveolar bone, dental arch and buccolingual inclination angle of the corresponding teeth in the maxillary and mandibular canine, premolar and molar areas.

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel activates the AldoA/HIF-1α pathway to promote diabetic wound healing.

Objectives: To identify the effect of adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber (ADSC-loaded β-ChNF) hydrogel on diabetic wound healing and clarify its mechanism of action.

Methods: We prepared the ADSC-loaded β-ChNF hydrogel to repair wounds of db/db diabetic mice. Wound healing rate, histopathology, enzyme-linked immunosorbent assay, and western blot were used to confirm its role and mechanism in promoting diabetic wound healing.

[Effect of inhibition of Porphyromonas endodontalis on osteoblast differentiation].

Purpose: Porphyromonas endodontalis (P.e) is the dominant bacterium in the infected canal of pulpal and periapical disease.Lipopolysaccharides (LPS) in the outer membrane of the cell wall is an important toxicity factor of P.

[Resveratrol counteracts lipopolisaccharide-mediated oxidative stress by upregulating SOCS-1 production].

Purpose: To investigate the protective effect and mechanism of resveratrol on oxidative stress of MC3T3-E1.

Methods: The levels of reactive oxygen species in the cells were observed by fluorescence microscope and flow cytometry. The expression of SOCS-1 protein was detected by Western blot.

[Effect of premolars scissor bite on the sagittal position of mandible].

Purpose: To study the effect of adolescent premolar scissor bite on the sagittal position of mandible and provide proper reference for correct orthodontic diagnosis and right time to treatment. METHODS： One hundred adolescents (54 females, 46 males) with scissor bite of premolars(70 were unilateral,30 were bilateral) were selected as experimental group between 2004 to 2017 from the Department of Orthodontics, Stomatological Hospital of China Medical University. Among them, 54 females served as female group, 46 males served as male group; 30 were bilateral and 70 were unilateral.

[Application of Flipped classroom combined with case-based learning in resident training of endodontics].

Purpose: To apply Flipped classroom combined with case-based learning(CBL) in resident training of endodontics, in order to improve training efficiency.

Methods: Fifty-one residents from China Medical University, School of Stomatology were randomly divided into 2 groups, twenty-six students in the experimental group were trained with Flipped classroom combined with CBL, the other twenty-five students in the control group were trained with traditional teaching method. At the end of the course, exams and questionnaires were conducted to evaluate the training quality of two different methods.

[Meta-analysis of the condylar position with mandibular deviation].

Purpose: This study was aimed to analyze the differences of condylar position between the mandibular deviation and the individual normal occlusion.

Methods: Databases of PubMed,Embase,CNKI ,Wanfang ,VIP and CBL were searched for the relevant articles about condylar position with mandibular deviation. The deadline was June 2017.

[Construction of cell sheets of stem cells from apical papilla and characterization of their biological properties].

Purpose: Cell sheets of stem cells from apical papilla (SCAP) were constructed. Their histological and biological properties were studied to provide experimental basis for its application in dental pulp regeneration in immature permanent teeth.

Methods: SCAP were isolated and cultured from immature permanent third molars.

[Effects of Porphyromonas endodontalis lipopolysaccharides on the expression of monocyte chemotactic protein-1 in mouse osteoblasts].

Purpose: To investigate the effects of lipopolysaccharide (LPS) extracted from Porphyromonas endodontalis (P.endodontalis) on expression of monocyte chemotactic protein-1 (MCP-1) mRNA and protein in MC3T3-E1 cells and the role of p38 mitogen-activated　protein　kinase (MAPK) and nuclear factor-kB(NF-kB）in the process.

Methods: MC3T3-E1 cells were treated with different concentrations of P.

[Clinical observation on different kinds of laser on 60 molars with abrasion].

Purpose: To evaluate the clinical effect of different kinds of laser on 60 molars with abrasion.

Methods: Thirty patients with 60 abrasive molars were selected according to the inclusion criteria. Molars and premolars were divided into 2 groups randomly.

[Effect of the thickness of reinforced glass ceramics on the degree of conversion of dual-cure resin cements].

Purpose: To determine the effect of the thickness of reinforced glass ceramics on the degree of conversion (DC) of three dual-cure resin cements (Multilink N, RelyX Ultimate and NX3-Nexus).

Methods: Upcera reinforced glass ceramics and IPS e.max CAD test specimen were processed, with different thickness, transmittance was tested by ultraviolet and visible spectrometer.

[Antimicrobial effect of a new bio-ceramic material iRoot FM on Porphyromonas endodontalis].

Purpose: To compare the antimicrobial effect of a new bio-ceramic material iRoot FM with traditional intracanal medicaments including calcium hydroxide [Ca(OH)] and triple antibiotic paste (TAP), on Porphyromonas endodontalis (P. endodontalis), and to evaluate the antimicrobial activity of iRoot FM, providing reference for clinical use of intracanal medicaments.

Methods: P.

Expression of E-cadherin, vimentin and β-catenin in ameloblastoma and association with clinicopathological characteristics of ameloblastoma.

Objective: Ameloblastoma shows invasive growth and is susceptible to recurrence. This study aimed to detect the expression of E-Cadherin, Vimentin and β-Catenin (proteins related to epithelial mesenchymal transformation (EMT) in the ameloblastoma and to explore association with clinicopathological characteristics of ameloblastoma.

Methods: Immunohistochemistry was employed to detect the protein expression of E-Cadherin, Vimentin and β-Catenin in the ameloblastoma, and its association with clinicopathological characteristics of ameloblastoma was further evaluated.

[Effects of lipopolysaccharides from Porphyromonas endodontalis on the expression of MIP-1α in mouse osteoblasts].

Purpose: To investigate the effects of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e) on the expression of macrophageinflammatoryprotein-1α (MIP-1α) mRNA and protein levels in MC3T3-E1 cells and the influence of curcumin in the process.

Methods: MC3T3-E1 cells were treated with 20 mg/L P.

[Effect of lipopolysaccharides from Porphyromonas endodontalis on the expression of interleukin-34 in mouse osteoblasts].

Purpose: To investigate the effects of lipopolysaccharides (LPS) extracted from Porphyromonas endodontalis (P.e) on the expression of interleukin-34 (IL-34) mRNA in MC3T3-E1 cells and the role of p38MAPK, ERK1/2, NF-κB and SIRT1 in the process.

Methods: MC3T3-E1 cells were treated with different concentrations of P.

[Application of PBL combined with SP method in during-course practice of endodontics for undergraduate dental students].

Purpose: To apply problem-based learning (PBL) combined with standardized patients(SP) in during-course practice of endodontics for undergraduate dental students, in order to improve the teaching quality.

Methods: One hundred and four undergraduate dental students of China Medical University School of Stomatology were randomly divided into 2 groups, 52 students in each group. One group were taught with PBL combined with SP while the other group with lecture-based learning (LBL) alone.

[The improvement of periodontal teaching mode based on PDCA theory].

Purpose: To evaluate the effect of PDCA teaching mode on clinical ability in the process of periodontal clinical internship.

Methods: Forty-eight undergraduate interns coming from School of Stomatology, China Medical University were divided into 2 groups, one group received traditional teaching mode, the other group received a teaching mode based on PDCA cycle. At the end of internship, every student was assessed by theoretical examinations, case reports and clinical skill practice.

[Comparison of wear resistance and flexural strength of three kinds of bulk-fill composite resins].

Purpose: To compare the abrasion resistance and flexure strength of three bulk-fill resin composites with an universal nano-hybrid composite resins.

Methods: The specimens were prepared with three kinds of bulk fill composites (SDR , sonicfill, Tetric N-Ceram Bulk Fill) and an universal nano-hybrid composite resins(Herculite Precis). 10 mm in diameter × 2mm in height specimens were prepared for abrasion resistance, while 2 mm in width × 2 mm in depth×25 mm in length specimens were prepared for flexure strength.

Evaluation of the efficacy and safety of topical timolol maleate combined with oral propranolol treatment for parotid mixed infantile hemangiomas.

The aim of the present study was to assess the efficacy and safety of topical timolol maleate combined with oral propranolol for parotid infantile hemangiomas. Between October 2012 and April 2014, propranolol was administered orally at a dose of 1.0-1.

[Effectiveness of Nd:YAG laser with non-surgical periodontal therapy on improvement of periodontitis: meta analysis].

Purpose: To compare the effects of premolars restored with 2 different types of fiber post systems, so as to provide experience for restoration of residual crown of premolars.

Methods: Fifty-three residual crowns of premolar restored with fiber post systems were collected, and randomly divided into 2 groups: parallel fiber post group and double taper fiber post group. Repairing effect and operation difficulty were compared.

[Comparison of the shaping ability of three Ni-Ti rotary instruments in the preparation of simulated curved root canals].

Purpose: To compare the shaping ability of three rotary Ni-Ti instruments in simulated root canals.

Methods: A total of 30 simulated resin blocks were divided randomly into 3 groups: ProTaper Universal, ProTaper Next and TF Adaptive. Each group consisted of 10 root canals.

[Experimental study on the retentive force of cobalt-chromium alloy, pure titanium and vitallium cast clasps in the simulated 3-year clinical use].

Purpose: To investigate the changes of retentive force of cobalt-chromium alloy, pure titanium and vitallium cast clasps in the simulated 3-year clinical use.

Methods: Fifteen metal abutment crowns made of No.QT800-2 nodular cast iron were used in the test.

MTSS1 gene regulated by miR-96 inhibits cell proliferation and metastasis in tongue squamous cellular carcinoma Tca8113 cell line.

Background: Metastasis suppressor-1 (MTSS1) is a novel potential metastasis suppressor gene in several types of human cancers. However, the exact function and regulatory mechanism of MTSS1 in Tongue squamous cellular carcinoma (TSCC) have not been elucidated.

Material/methods: We first confirmed the MTSS1 gene expression by using quantitative real time-PCR (qRT-PCR) and immunohistochemical staining.

[Root canal treatment of mandibular first premolar with 4 root canals: a case report].

The mandibular first premolar can be considered one of the most challenging teeth to treat, due to the complexity of its root canal morphology and increased incidence of multiple canals. A case of endodontic treatment of a mandibular first premolar exhibiting a total of 4 distinct root canals and 4 apical foramina was described. Anatomic variation of root canal morphology should be considered in endodontic treatment to ensure a favorable healing outcome, and its identification could be enhanced by careful examination using a dental operating microscope.