14 results match your criteria: "China Graduate School of Peking Union Medical College[Affiliation]"
Zhonghua Yu Fang Yi Xue Za Zhi
September 2024
Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, China.
To express and purify the phage depolymerase from hypervirulent (hv) serotype K1 and validate its function. Phage that infected serotype K1-type hv was isolated from hospital sewage. The biology and morphology of the phage were determined by plaque assay and transmission electron microscopy.
View Article and Find Full Text PDFZhonghua Yu Fang Yi Xue Za Zhi
July 2024
Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, China.
To investigate the effects of gene deletion in on capsule formation ability and bacteriophage sensitivity. The deletion mutant strain was constructed through a temperature-sensitive plasmid-mediated homologous recombination. The growth curves of W14 and Δ were detected by measuring the optical density OD.
View Article and Find Full Text PDFAging Med (Milton)
June 2024
Department of Anesthesiology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine Chinese Academy of Medical Sciences Beijing China.
Objectives: We hope to offer a comprehensive understanding of the advancements and patterns in research on PND. Methods: We performed a thorough search on the Web of Science Core Collection to locate relevant studies published from 1969 to 2022 and utilized four distinct tools, namely VOSviewer (J Data Inf Sci, 2017, 2, 1; J Am Soc Inf Sci, 1973, 24, 265; Amer Doc, 1963, 14, 10 and Scientometrics, 2010, 82, 581), CiteSpace (Scientometrics, 2010, 84, 523), Scimago Graphica, and R-bibliometrix which allowed us to examine various aspects. Results: We included a total of 6787 articles and reviews for analysis which described PND research, the sources, and the subfields; highlighted the significant developments in this field; identified three main directions in PND.
View Article and Find Full Text PDFZhonghua Yu Fang Yi Xue Za Zhi
June 2024
Department of Allergy, Children's Hospital Capital Institute of Pediatrics, Beijing 100020, China Graduate School of Peking Union Medical College, Beijing 100730, China.
To analyze the clinical features and risk factors of chest tightness variant asthma (CTVA) in children, so as to provide basis for the prevention and management of the disease. A cross-sectional study was conducted to analyze 178 children aged 6-17 years old who were admitted to the Department of Allergy, Capital Institute of Pediatrics Affiliated Children's Hospital from January 2021 to January 2023 due to chest tightness. The age was 8.
View Article and Find Full Text PDFZhonghua Yi Xue Za Zhi
October 2023
Department of Respiratory and Critical Care Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, China.
To explore the correlation between pulmonary quantitative CT measurement indicators and respiratory symptoms in patients with stable chronic obstructive pulmonary disease (COPD). A total of 186 patients with COPD in stable stage who visited in the outpatient department of Beijing Hospital from March 2021 to February 2022 were prospectively included. Demographic data, respiratory symptoms and lung function were collected.
View Article and Find Full Text PDFZhonghua Yu Fang Yi Xue Za Zhi
May 2023
Department of Respiratory and Critical Care Medicine, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital, Beijing 100730, China Graduate School of Peking Union Medical College, Beijing 100730, China.
To explore the characteristics, patterns of multimorbidity and the impact on quality of life and the prognosis of middle-aged and elderly patients with chronic obstructive pulmonary disease (COPD). This is a cross-sectional study. From January 2012 to December 2021, 939 middle-aged and elderly COPD patients hospitalized in Beijing Hospital were selected by the convenient sampling method.
View Article and Find Full Text PDFZhonghua Yu Fang Yi Xue Za Zhi
April 2022
Department of Gynecology and Obstetrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, China Graduate School of Peking Union Medical College, Beijing 100730, China.
To analyze the type and distribution characteristics of human papillomavirus (HPV) infection along with cervical cytology in middle-aged and elderly women in Guangxi and to provide a basis for the prevention and treatment of cervical cancer in elderly women. 21 subtypes of HPV and cervical cytology of women over 45-year-old visiting the First Affiliated Hospital of Guangxi Medical University from January 2019 to December 2020 were collected. They were divided into two groups by age, 45-64 years group and over 65 years group.
View Article and Find Full Text PDFZhonghua Liu Xing Bing Xue Za Zhi
May 2021
Department of Endocrinology/Department of Radiology, Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, China Graduate School of Peking Union Medical College, Beijing 100005, China.
To evaluate the characteristics of adult abdominal fat distribution and analyze its influencing factors by energy spectrum CT scan. The body height, weight, waist circumference, and hip circumference of 105 adults were measured, and the characteristics of abdominal fat distribution were evaluated by energy spectrum CT scan. Compared with non-obese individuals, the contents of abdominal subcutaneous fat, abdominal cavity and liver ectopic fat were higher in obese patients (<0.
View Article and Find Full Text PDFPak J Pharm Sci
September 2016
Research Center of Biomaterial and Novel Drug Delivery Systems, National Research Institute for Family Planning, Da Hui Si Haidian District, Beijing, PR China.
Preparation and in vitro/in vivo evaluation of gestodene (GEST) intravaginal ring (IVR) formulations which can release a constant dose of GEST during 3 weeks were investigated. In present study a reservoir gestodene intravaginal ring, including a gestodene silicone elastomer core and the non-active silicone layer, was reported, which was manufactured by reaction injection moulding at 80°C for 20 min. The raw materials compatibility experiments showed that the silicone elastomer core carrier wouldn't interact with drugs.
View Article and Find Full Text PDFMol Hum Reprod
September 2016
Center for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Haidian District, Beijing 100084, China
Study Question: Does a heterozygous mutation in AMHR2, identified in whole-exome sequencings (WES) of patients with primary ovarian insufficiency (POI), cause a defect in anti-Müllerian hormone (AMH) signaling?
Summary Answer: The I209N mutation at the adenosine triphosphate binding domain of AMHR2 exerts dominant negative defects in the AMH signaling pathway.
What Is Known Already: Previous studies have demonstrated the associations of several sequence variants in AMH or AMHR2 with POI, but no functional assay has been performed to verify whether there was any defect on AMH signaling.
Study Design, Samples/materials, Methods: Ninety-six unrelated female Chinese Han patients were diagnosed with idiopathic POI and subjected to WES.
Plant Cell
May 2016
National Institute of Biological Sciences, Beijing 102206, China Graduate School of Peking Union Medical College, Beijing 100730, China
The mechanism by which MORPHEUS' MOLECULE1 (MOM1) contributes to transcriptional gene silencing has remained elusive since the gene was first identified and characterized. Here, we report that two Arabidopsis thaliana PIAS (PROTEIN INHIBITOR OF ACTIVATED STAT)-type SUMO E3 ligase-like proteins, PIAL1 and PIAL2, function redundantly to mediate transcriptional silencing at MOM1 target loci. PIAL1 and PIAL2 physically interact with each other and with MOM1 to form a high molecular mass complex.
View Article and Find Full Text PDFHum Reprod
September 2015
Reproductive Physiology Laboratory, National Research Institute for Family Planning, No.12 Dahuisi Road, Haidian District, Beijing 100081, People's Republic of China.
Study Question: How is vascular endothelial growth factor (VEGF) expression regulated by hypoxia inducible factor 1 alpha (HIF1A) during menstruation?
Summary Answer: After progesterone (P4) withdrawal, HIF1A was activated and it directly up-regulated VEGF mRNA expression and this regulation was the highest during endometrium breakdown in the mouse menstrual-like model.
What Is Known Already: VEGF, an important angiogenic factor, is known to be essential for endometrial repair, particularly in angiogenesis and re-epithelialization. However, its upstream regulation has not been fully clarified.
J Virol
January 2015
Molecular Immunology Laboratory, Capital Institute of Pediatrics, Beijing, China Graduate School of Peking Union Medical College, Capital Institute of Pediatrics, Beijing, China
Unlabelled: Coxsackievirus B3 (CVB3) is trophic for cardiac tissue and is a major causative agent for viral myocarditis, where local viral replication in the heart may lead to heart failure or even death. Recent studies show that inserting microRNA target sequences into the genomes of certain viruses can eradicate these viruses within local host tissues that specifically express the cognate microRNA. Here, we demonstrated both in vitro and in vivo that incorporating target sequences for miRNA-133 and -206 into the 5' untranslated region of the CVB3 genome ameliorated CVB3 virulence in skeletal muscle and myocardial cells that specifically expressed the cognate cellular microRNAs.
View Article and Find Full Text PDFZhonghua Liu Xing Bing Xue Za Zhi
April 2014
Department of Epidemiology, Capital Institute of Pediatrics, Beijing 100020, China. Email:
Objective: To analyze the trends on the prevalence rates of obesity and cardiometabolic among children and adolescents in Beijing, during 2004-2013.
Methods: Data was collected from three cross-sectional studies among children and adolescents, aged 7-17 years old in Beijing. Two studies in 2004 and 2013 were conducted in general population, and one was among obese children in 2007.