Analysis and identification of potential biomarkers for dysfunctional uterine bleeding.

Clinical evidence increasingly suggests that traditional treatments for dysfunctional uterine bleeding (DUB) have limited success. In this study, blood samples from 10 DUB patients and 10 healthy controls were collected for transcriptome sequencing. Then, the differentially expressed genes (DEGs) were screened and crossed with the DUB-related module genes to obtain the target genes.

Human embryo implantation: The complex interplay between endometrial receptivity and the microbiome.

The endometrial and vaginal microbiota have co-evolved with the reproductive tract and play a key role in both health and disease. However, the difference between endometrial and vaginal microbiota, as well as their association with reproductive outcomes in women undergoing frozen embryo transfer, remains unclear. 120 women who underwent in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) and whole embryo freezing were enrolled.

Inhibition mechanism of Microcystis aeruginosa in coculture of Lemna and Azolla: Insights from non-targeted Metabonomics.

Microcystis aeruginosa, a harmful alga in cyanobacterial blooms, damages aquatic ecosystems. Species diversity may control the blooms by increasing ecosystem stability and resource utilization. The growth and photosynthetic systems of M.

Multiple insights into differential Cd detoxification mechanisms in new germplasms of mung bean (Vigna radiata L.) and potential mitigation strategy.

Long-term cadmium (Cd) exposure inhibits plant growth and development, reduces crop yield and quality, and threatens food security. Exploring the Cd tolerance mechanisms and safe production of crops in Cd-contaminated environment has become a worldwide concern. In this study, mung bean (Vigna radiata L.

Comprehensive characterization of the bHLH transcription factor family in Curcuma wenyujin and functional elucidation of CwbHLH27 in jasmonate-regulated sesquiterpenoid biosynthesis.

Curcuma wenyujin is acknowledged as a crucial medicinal plant containing essential oils, primarily composed of sesquiterpenoids. While numerous sesquiterpenoids exhibit versatile physiological activities, their levels in C. wenyujin are generally low, particularly the pivotal anti-cancer component elemene.

Alginate oligosaccharide induces resistance against Penicillium expansum in pears by priming defense responses.

The research intended to explore the control ability of alginate oligosaccharide (AOS) on Penicillium expansum infection in pear fruit by priming response and its mechanism. The results showed that 100 mg L AOS treatment could significantly reduce the incidence of postharvest blue mold and the lesion diameter in pear fruits and maintain their quality. The defense responses induced by AOS treatment alone were relatively mild in pear fruits.

How do trees fail in intraspecific competition? A test for the roles of non-structural carbohydrates and stoichiometries in Pinus massoniana.

Competition is ubiquitous and an important driver of tree mortality. Non-structural carbohydrates (NSCs, including soluble sugars and starch) and C-N-P stoichiometries are affected by the competitive status of trees and, in turn, physiologically determine tree growth and survival in competition. However, the physiological mechanisms behind tree mortality caused by intraspecific competition remain unclear.

The basic helix-loop-helix transcription factor PpeUNE12 regulates peach ripening by promoting polyamine catabolism and anthocyanin synthesis.

The basic helix-loop-helix (bHLH) transcription factors (TFs) play important roles in various plant developmental and biological processes. However, the precise mechanisms by which bHLH TFs regulate fruit ripening warrant further investigation. Polyamine oxidase (PAO) is crucial for polyamine (PA) catabolism and plays crucial roles in fruit ripening.

Influence of family socioeconomic status on academic buoyancy and adaptability: Mediating effect of parental involvement.

Prior studies highlight the importance of academic buoyancy and adaptability in educational trajectories, yet the influence of family-related factors remains less explored. Anchored in Bronfenbrenner's ecological systems theory, this research examines how family socioeconomic status (SES) influences academic buoyancy and adaptability, the predictive relation between family SES and parental involvement, and whether parental involvement mediates the impact of family SES on academic outcomes. We surveyed 1164 junior high school students from China.

A study on how to improve students' continuance intention in landscape architecture conservation course within a virtual learning environment based on SVVR.

With the rapid pace of global urbanization, preserving natural landscapes has become increasingly critical. However, urbanization presents significant environmental risks worsened by decreased ecological consciousness. This has led to a pressing demand for education in landscape conservation.

Investigating the relationship between goal orientation, self-efficacy, positive emotionality, and affective engagement among Chinese students.

This study delved into the complex interplay between self-efficacy, achievement goals, positive emotions, and affective engagement among university students in China. To achieve this, a stratified random sampling technique was employed, resulting in a sample of 391 students from four geographically distinct universities across China. The data collection relied on self-reported questionnaires that measured academic self-efficacy, goal orientation (specifically focusing on mastery versus performance goals), positive emotions, and various aspects of affective engagement, including enjoyment, satisfaction, and interest in learning.

Melatonin protects aged oocytes from depalmitoylation-mediated quality reduction by promoting PPT1 degradation and antioxidation.

Oocyte aging is closely related to a decline in female fertility, accompanied by increased reactive oxygen species levels and changes in protein posttranslational modifications. However, the role of protein palmitoylation in oocyte aging has not been investigated. In the present study, a new association between redox and palmitoylation in aging oocytes was found.

Ambient coarse particulate matter pollution and hospital admissions for schizophrenia.

Objective: To investigate the association between ambient coarse particulate matter (PM) pollution and risk of acute schizophrenia episodes.

Methods: A time-stratified case-crossover study with a two-stage analytical approach was conducted to investigate the association between ambient PM pollution and schizophrenia admissions (an indicator for acute schizophrenia episodes) across 259 Chinese cities of prefecture-level or above during 2013-2017. A conditional logistic regression model was constructed to estimate city-specific changes in hospital admissions for schizophrenia associated with per interquartile range (IQR) increase in ambient PM, and the overall associations were obtained by pooling the city-specific associations using the random-effects model.

A preliminary study of microwave ablation (MWA) for Bethesda IV follicular neoplasms (≤3 cm).

Objective: To evaluate the feasibility, safety, and efficacy of microwave ablation (MWA) for the treatment of patients with Bethesda IV follicular neoplasms (FNs) (≤3 cm).

Methods: In the retrospective study, patients who underwent MWA for Bethesda IV follicular neoplasms (≤3 cm) were included. Technical success, volume reduction, disease progression, and adverse event (AE) rates were analyzed postablation.

HemaScope: A Tool for Analyzing Single-cell and Spatial Transcriptomics Data of Hematopoietic Cells.

Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) techniques hold great value in evaluating the heterogeneity and spatial characteristics of hematopoietic cells within tissues. These two techniques are highly complementary, with scRNA-seq offering single-cell resolution and ST retaining spatial information. However, there is an urgent demand for well-organized and user-friendly toolkits capable of handling single-cell and spatial information.

Protocol for alleviating alcohol-induced conditioned place preference in mice using alternating bilateral sensory stimulation.

Alternating bilateral sensory stimulation (ABS) is a clinical physical therapy technique effective in treating post-traumatic stress disorder (PTSD). However, its utilization in treating conditions beyond PTSD remains limited. Here, we present a protocol to reduce ethanol-induced conditioned place preference (CPP) using 4 Hz ABS.

Protocol for identifying Dicer as dsRNA binding and cleaving reagent in response to transfected dsRNA.

Mammalian Dicer has been proved to be functional on double-stranded RNAs (dsRNAs) and involved in antiviral immunity or immune regulation. Here, we present a protocol for identifying Dicer as a dsRNA binding and cleaving factor to transfected dsRNA in cell lines, based on small RNA sequencing (RNA-seq) and dsRNA-immunoprecipitation (dsRNA-IP). We detail both experimental processes and analysis on small RNA-seq data.

Protocol for extraction of gut interstitial fluid in mice with two-front nutrient supply.

The intestine features a two-front nutrient supply environment, comprising an enteral side enriched with microbial and dietary metabolites and a serosal side supplied by systemic nutrients, collectively supporting intestinal and systemic homeostasis, but there is currently no optimal approach for extracting and assessing the local intestinal microenvironment. Here, we present a protocol for constructing a nutrient supply model in mice and extracting gut interstitial fluid (GIF) via centrifugation. This model and the extracted GIF are suitable for downstream analyses.

Epiregulin ameliorates ovariectomy-induced bone loss through orchestrating the differentiation of osteoblasts and osteoclasts.

Epiregulin plays a role in a range of biological activities including malignancies. This study aims to investigate the potential contribution of epiregulin to bone cell differentiation and bone homeostasis. The data showed that epiregulin expression was upregulated during osteogenesis but downregulated during adipogenesis.

Targeting the IKZF1/BCL-2 axis as a novel therapeutic strategy for treating acute T-cell lymphoblastic leukemia.

Objectives: Acute T-cell lymphoblastic leukemia (T-ALL) is a severe hematologic malignancy with limited treatment options and poor long-term survival. This study explores the role of IKZF1 in regulating BCL-2 expression in T-ALL.

Methods: CUT&Tag and CUT&Run assays were employed to assess IKZF1 binding to the BCL-2 promoter.

Unveiling the Dark Side of Flavonoid: Rutin Provokes Hepatotoxicity in Low-Dose 2-Amino-3-methylimidazo [4,5-] Quinoline-Exposed Mice via Regulating Gut Microbiota and Liver Metabolism.

2-Amino-3-methylimidazole [4,5-] quinoline (IQ) is a kind of heterocyclic amine (HCAs) with high carcinogenicity in hot processed meat. Rutin (Ru) is a flavonoid compound with anti-inflammatory and antioxidant properties. However, whether Ru is scatheless under IQ-stimulated potential unhealthy conditions, especially liver function, in vivo, is unknown.

Predictive value of insulin resistance metabolic score for cardiovascular disease in Chinese arthritis patients: a prospective cohort study.

Objectives: Although patients with arthritis have significantly increased cardiovascular disease (CVD) risk, effective prediction tools remain limited. This study aimed to evaluate the predictive value of the Metabolic Score for Insulin Resistance (METS-IR) for CVD events among Chinese patients with arthritis.

Methods: Using data from the China Health and Retirement Longitudinal Study (CHARLS), we conducted a 7-year prospective cohort study (2011-2018) involving 1,059 patients with arthritis.

doubletrouble: an R/Bioconductor package for the identification, classification, and analysis of gene and genome duplications.

Summary: Gene and genome duplications are major evolutionary forces that shape the diversity and complexity of life. However, different duplication modes have distinct impacts on gene function, expression, and regulation. Existing tools for identifying and classifying duplicated genes are either outdated or not user-friendly.

Effects of Probiotics and Diet Management in Patients With Irritable Bowel Syndrome: A Systematic Review and Network Meta-analysis.

Context: The efficacy of probiotics and diet management in irritable bowel syndrome (IBS) is controversial, and their relative effectiveness remains unclear.

Objective: This study aimed to evaluate the effects of probiotics, diet management, and their combination on IBS.

Data Sources: PubMed, Embase, Cochrane, and Web of Science were searched from inception to July 10, 2023, for relevant studies, including symptom relief, IBS-symptom severity score (-SSS), and IBS-quality of life measure (-QOL).