Praesto Jetted A50 HipH, a mild pH elution Protein A resin, exhibits improved aggregate separation capability and protein elution from it shows unique response to mobile phase additive sodium chloride.

Protein A affinity chromatography has been widely used for product capture in monoclonal antibody (mAb), bispecific antibody (bsAb) and Fc-fusion protein purification. However, the low pH (i.e.

Dandelion extract suppresses the stem-like properties of triple-negative breast cancer cells by regulating CUEDC2/β-catenin/OCT4 signaling axis.

Ethnopharmacological Relevance: Triple-negative breast cancer (TNBC) represents the most aggressive subtype of breast cancer, featuring a high proportion of cancer stem cells (CSCs) and the poorest clinical outcomes. Taraxacum mongolicum Hand. -Mazz.

Ribes diacanthum Pall modulates bile acid homeostasis and oxidative stress in cholestatic mice by activating the SIRT1/FXR and Keap1/Nrf2 signaling pathways.

Ethnopharmacological Relevance: Cholestatic liver injury (CLI) is a pathophysiological syndrome characterized by the accumulation of bile acids (BAs), which leads to significant hepatic dysfunction. This condition is frequently associated with disturbances in BAs homeostasis and the induction of oxidative stress. Ribes diacanthum Pall (RDP), a conventional folk medicinal plant, has been employed in Mongolia, the Inner Mongolia region of China, and other areas for the remediation of hepatic disorders.

FDuDoCLNet: Fully dual-domain contrastive learning network for parallel MRI reconstruction.

Magnetic resonance imaging (MRI) is a non-invasive medical imaging technique that is widely used for high-resolution imaging of soft tissues and organs. However, the slow speed of MRI imaging, especially in high-resolution or dynamic scans, makes MRI reconstruction an important research topic. Currently, MRI reconstruction methods based on deep learning (DL) have garnered significant attention, and they improve the reconstruction quality by learning complex image features.

Neuroimaging features for cognitive fatigue and its recovery with VR intervention: An EEG microstates analysis.

Introduction: Cognitive fatigue is mainly caused by enduring mental stress or monotonous work, impairing cognitive and physical performance. Natural scene exposure is a promising intervention for relieving cognitive fatigue, but the efficacy of virtual reality (VR) simulated natural scene exposure is unclear. We aimed to investigate the effect of VR natural scene on cognitive fatigue and further explored its underlying neurophysiological alterations with electroencephalogram (EEG) microstates analysis.

JAK/STAT3 signaling promotes pain and depression-like behaviors in rats with bone cancer pain by regulating Th17 cell differentiation.

Background: Pain and depression are common complications in patients with advanced cancer, which significantly affects their quality of life and survival. Dysregulation of the JAK/STAT3 pathway in the central nervous system is associated with pain and brain inflammatory disorders, but its role in bone cancer pain (BCP) remains unclear. This study aimed to investigate the specific role of the JAK/STAT3 pathway in the amygdala in BCP.

FOXS1, frequently inactivated by promoter methylation, inhibited colorectal cancer cell growth by promoting TGFBI degradation through autophagy-lysosome pathway.

Introduction: Tumor suppressor gene (TSG) inactivation by epigenetic modifications contributes to the carcinogenesis and progression of colorectal cancer (CRC). Expression profiling and CpG methylomics revealed that a forkhead-box transcriptional factor, FOXS1, is downregulated and methylated in CRC.

Objectives: To assess the biological functions and underlying mechanisms of FOXS1 in colorectal cancer.

Ailanthone induces triple-negative breast cancer cells death involving the inhibition of OTUB1-mediated ERRα deubiquitylation.

Introduction: Triple-negative breast cancer (TNBC) remains the most aggressive subtype of breast cancer, and effective therapeutic strategies are needed. Estrogen-related receptor alpha (ERRα) is considered a promising target for managing TNBC.

Objectives: Here, we aimed to screen natural products to find downregulator of ERRα and elucidate its mechanism of action.

The Effect of Resveratrol on Lead-Induced Oxidative Damage and Apoptosis in HT-22 Cells.

Objective: The purpose of this work was to investigate whether resveratrol affects lead-induced oxidative damage in HT-22 cells, characterizing mechanisms and strategies for preventing and treating lead-induced neurotoxicity.

Methods: Various lead and resveratrol concentrations were applied to HT-22 cells over different time periods. First, we established the lead treatment (12.

A New target of ischemic ventricular arrhythmias-ITFG2.

ITFG2 is an intracellular protein known to modulate the immune response of T-cells. Our previous investigation revealed that ITFG2 specifically targets ATP5b to regulate ATP energy metabolism and maintain mitochondrial function, thereby protecting the heart from ischemic injury. However, the role of ITFG2 in ischemic ventricular arrhythmias and its underlying mechanisms have not been previously reported.

Hirsutine Mitigates Ferroptosis in Podocytes of Diabetic Kidney Disease by Downregulating the p53/GPX4 Signaling Pathway.

Diabetic kidney disease (DKD) is a leading cause of chronic kidney disease worldwide, and podocyte ferroptosis plays a crucial role in its pathogenesis. Hirsutine (HS) reduces blood glucose levels and improve insulin resistance in diabetic mice, suggesting its potential use in diabetes treatment. Here, we established a db/db mouse model of DKD and administered HS for 8 weeks.

Association of VEGFR2 Polymorphisms with Clinical Outcomes of Anti-angiogenesis Therapy in Cancer Patients: A Systematic Review and Meta-analysis.

Background: Some cancer patients derive limited benefit from anti-angiogenic therapy or discontinuation due to adverse reactions. Vascular endothelial growth factor receptor 2 (VEGFR2) plays an important role in regulating angiogenesis in tumors. This study aims to evaluate the association of VEGFR2 polymorphisms with clinical outcomes of anti-angiogenic drugs (AADs) in cancer patients.

Decoding Cortical Folding Patterns in Marmosets Using Machine Learning and Large Language Model.

Macroscale neuroimaging results have revealed significant differences in the structural and functional connectivity patterns of gyri and sulci in the primate cerebral cortex. Despite these findings, understanding these differences at the molecular level has remained challenging. This study leverages a comprehensive dataset of whole-brain in situ hybridization (ISH) data from marmosets, with updates continuing through 2024, to systematically analyze cortical folding patterns.

Mitigating LPS-induced stress in Chinese mitten crab (Eriocheir sinensis) with P4' peptide-bearing Bacillus subtilis.

The Chinese mitten crab (Eriocheir sinensis) is an important component in Chinese aquaculture. Due to its lacking adaptive immune system as a crustacean, it exhibits poor tolerance to environmental stresses, particularly the deleterious impact of lipopolysaccharide (LPS) from pathogenic bacteria during E. sinensis culture.

MicroRNA-203-3p participates in antiviral immune response by negatively regulating TRAF3 in the rainbow trout (Oncorhynchus mykiss).

MicroRNAs (miRNAs) are highly conserved endogenous non-coding RNAs that play a crucial role in fish immune response by regulating gene expression at the post-transcriptional level. In recent years, the viral diseases caused by infectious hematopoietic necrosis virus (IHNV) have caused significant economic losses in rainbow trout (Oncorhynchus mykiss) aquaculture, whereas the immune regulatory mechanisms of miRNAs involved in rainbow trout resistance to IHNV infection remains largely undefined. In this study, we analyzed the structural characteristics of Oncorhynchus mykiss tumor necrosis factor receptor-associated factor 3 (OmTRAF3) by bioinformatics software and explored the molecular mechanism of miR-203-3p in rainbow trout resistance to IHNV by regulating OmTRAF3 in vivo and in vitro.

Rahnella aquatilis VgrG-mediated PANoptosis in macrophages of Carassius auratus by dual RNA-seq analysis.

Rahnella aquatilis is an emerging opportunistic pathogen that usually causes septicaemia in fish and poses a potential threat to human health. VgrG gene is an important virulence factor of type VI secretion system in R. aquatilis, but its regulatory mechanism underlying PANoptosis is still unknown.

Modular metabolic flux control for kick-starting cascade catalysis through engineering customizable compartment.

Microbial compartment provides a promising approach for achieving high-valued chemical biosynthesis from renewable feedstock. However, volatile precursor could be utilized by pathway enzyme, which may hinder and adverse the cascade catalysis within microbial cell factory. Here, a customizable compartment was developed for pathway sequestration using spatially assembled cascade catalysis reaction.

Design, Current States, and Challenges of Nanomaterials in Anti-Neuroinflammation: A Perspective on Alzheimer's Disease.

Alzheimer's disease (AD), an age-related neurodegenerative disease, brings huge damage to the society, to the whole family and even to the patient himself. However, until now, the etiological factor of AD is still unknown and there is no effective treatment for it. Massive deposition of amyloid-beta peptide(Aβ) and hyperphosphorylation of Tau proteins are acknowledged pathological features of AD.

Lactylation: The Metabolic Accomplice Shaping Cancer's Response to Radiotherapy and Immunotherapy.

Protein lactylation, an emerging post-translational modification, is providing new insights into tumor biology and challenging our current understanding of cancer mechanisms. Our review illuminates the intricate roles of lactylation in carcinogenesis, tumor progression, and therapeutic responses, positioning it as a critical linchpin connecting metabolic reprogramming, epigenetic modulation, and treatment outcomes. We provide an in-depth analysis of lactylation's molecular mechanisms and its far-reaching impact on cell cycle regulation, immune evasion strategies, and therapeutic resistance within the complex tumor microenvironment.

Overexpressing OsNF-YB12 elevated the content of jasmonic acid and impaired drought tolerance in rice.

Nuclear factor Y (NF-Y) is an evolutionarily conserved heterotrimeric transcription factor in eukaryotes. In a previous study, OsNF-YB12 was confirmed to be associated with drought tolerance using the Ecotilling method. In this study, real-time quantitative RT-PCR revealed that OsNF-YB12 was induced by various abiotic stresses and phytohormones, with expression levels differing between leaves and roots.

Lung cancer screening program quality indicators - review & recommendations - An International Association for the Study of Lung Cancer Delphi process study.

Introduction: Lung cancer screening (LCS) using low-dose-computed tomography reduces lung cancer mortality in high-risk individuals. Evaluating and monitoring LCS programs are important to ensure and improve quality, efficiency and participant outcomes. There is no agreement on LCS quality indicators (QIs).

Tumor-derived extracellular vesicle PD-1 promotes tumor immune evasion via disruption of peripheral T cell homeostasis.

The programmed cell death 1 (PD-1)/PD-1 ligand 1 (PD-L1) axis mediates immune evasion of tumor, and targeting this axis has achieved some clinical benefits. The regulation of PD-1 expression in immune cells has been well studied. However, whether any other potential source of immune cell-expressed PD-1 exists remains unknown.

Machine Learning-based Lactate-Related Genes Signature Predicts Clinical Outcomes and Unveils Novel Therapeutic Targets in Esophageal Squamous Cell Carcinoma.

Esophageal squamous cell carcinoma (ESCC), a predominant subtype of esophageal cancer, typically presents with poor prognosis. Lactate is a crucial metabolite in cancer and significantly impacts tumor biology. Here, we aimed to construct a lactate-related prognostic signature (LPS) for predicting prognosis in ESCC and uncovering potential therapeutic targets.