Deep eutectic solvent-enabled lignocellulosic biomass valorization: Toward understanding of biomass pretreatment, lignin dissolution, and lignin's antioxidant activity.

A comprehensive study was conducted to determine the effects of water and ethylene glycol (EG) on biomass pretreatment using a binary deep eutectic solvent (DES) containing choline chloride and acetic acid (1ChCl3AC) at a mole ratio of 1:3. Different quantities of water and EG were combined with 1ChCl3AC to pretreat wheat straw, miscanthus, eucalyptus, and sorghum stalk at 130 °C for 6 h. The changes in nanopore structure and surface roughness of wet biomass, as well as biomass crystallinity after 1ChCl3AC-based pretreatment were investigated using XRD and small-angle neutron scattering (SANS).

Preparation, characterization, and stability of pectin-whey protein isolate-based nanoparticles with mitochondrial targeting ability.

Quercetin (Que) is a polyhydroxy flavonoid with strong inhibitory activity against cancer cells. However, the poor water solubility and low bioavailability of Que. limit its application in the functional food industry.

Cofactor-directed co-immobilization of dual-enzyme on functionalized montmorillonite with enhanced catalytic performance.

Recently, multi-enzyme cascade catalysis has attracted increasing attention due to the advantages of integrating multiple enzymes, few side reactions and high catalytic efficiency. Herein, a novel dual-enzyme cascade system (GOx-FMt-HRP) was developed through cofactor-directed orientational co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) onto functional montmorillonite (FMt). The presented method realizes the reconstitution of cofactors and apo-enzymes (enzymes without cofactors), which enables enzymes to be immobilized in specific orientations on the support, thereby effectively reducing changes in their conformation.

Correlation between TCF7 and bladder cancer and feasibility of Erlotinib targeting in bladder cancer: Molecular mechanism and expression of TCF7 recombinant protein.

The primary objective of this study was to conduct a comprehensive analysis of the mechanism by which TCF7 recombinant protein operates, as well as to examine its expression patterns within bladder cancer cells. This research seeks to establish a new theoretical framework and provide experimental data that could advance the field of molecular targeted therapy for bladder cancer. Erlotinib, a well-known targeted therapy drug, was administered to the bladder cancer cells, and we evaluated its antitumor effects through various assays such as cell proliferation, apoptosis, and cell cycle analysis.

Emerging roles of hyaluronic acid hydrogels in cancer treatment and wound healing: A review.

Hyaluronic acid (HA)-derived hydrogels signify a noticeable development in biomedical uses, especially in cancer treatment and wound repair. Cancer continues to be one of the foremost causes of death globally, with current therapies frequently impeded by lack of specificity, serious side effects, and the emergence of resistance. HA hydrogels, characterized by their distinctive three-dimensional structure, hydrophilic nature, and biocompatibility, create an advanced platform for precise drug delivery, improving therapeutic results while minimizing systemic toxicity.

Purification, structural characterization, and in vitro immunomodulatory activity of a low-molecular-weight polysaccharide from cultivated Chinese cordyceps.

Cultivated Chinese cordyceps, an optimal substitute for the endangered wild resource, has recently been produced on a large scale. This work sought to explore the structural features and immunomodulatory activity of a novel low-molecular-weight polysaccharide (CSP1a, 15.7 kDa) isolated from cultivated Chinese cordyceps.

Konjac glucomannan-embedded corn starch-derived type 1 resistant starch: Physicochemical properties, in vitro digestibility and fermentation characteristics, and in vivo glucose response in mice.

Type 1 resistant starch (RS1) was prepared by high-pressure homogenization of corn starch (CS) embedded with 0.1 %, 0.3 %, 0.

Molecular mechanism of long chain non coding RNA LINC00511 influencing breast cancer stem cells: Mechanism of VEGFR1 protein.

A comprehensive investigation into the mechanism of VEGFR1 protein in this process was undertaken. Lentivirus-mediated RNA interference was employed to inhibit the expression of LINC00511 in breast cancer cell lines, and changes in breast cancer stem cell markers, including CD44+/CD24-, were monitored using flow cytometry. Additionally, the interaction between VEGFR1 protein and LINC00511 and the activation of its downstream signaling pathway were investigated through co-immunoprecipitation (Co-IP) and Western blot techniques.

Key epigenetic enzymes modulated by natural compounds contributes to tumorigenicity.

Dysregulation of epigenetic regulation is observed in numerous tumor cells. The therapeutic effects of natural products on tumors were investigated through a comprehensive analysis of active ingredients derived from various structured natural products. The analysis focuses on regulating key enzymes involved in epigenetic control.

Transcriptome-wide N-methyladenosinem modifications analysis of growth and fumonisins production in Fusarium proliferatum causing banana crown rot.

Crown rot caused by Fusarium proliferatum is a severe postharvest disease of banana fruit. The N-methyladenosine (mA) modification is the most common type of RNA modification and regulates gene expression in eukaryotes. Here, we analyzed transcriptome-wide changes in mA methylation to investigate post-transcriptional regulation mechanisms of growth and fumonisin biosynthesis of F.

An effective approach for lignin-based bamboo adhesive preparation via swelling crosslinking.

The preparation of lignin-based adhesives from sustainable lignin sources has garnered increasing attention from many researchers in recent years. However, developing high-performance and environmentally friendly lignin-based adhesives through a simple and efficient approach remains a significant challenge. In this study, aminated corn stover lignin (ACSL) was prepared by aminating corn stover lignin (CSL) using glutaraldehyde and ethylenediamine.

The mussel-inspired GelMA/dopamine/hyaluronic acid composite hydrogel dressing for wet surface adhesion.

Tissue adhesives have attracted wide attention as alternatives to sutures. Further developments in adhesives with excellent adhesion and biocompatibility for wet tissue surfaces are still required. This study provides a new solution for the development of bioadhesives for use on tissue surfaces under wet conditions.

Development of high-strength, 3D-printable, and biocompatible gelatin/κ-carrageenan dual-network hydrogels for wound healing.

Gelatin/κ-carrageenan (Gel/KC) hydrogel has exhibited a significant potential in tissue engineering, however, there is still a need to further enhance its structural properties. This study developed a Gel/KC dual-network hydrogel with superior mechanical properties and structural stability, which was integrated with 3D printing to evaluate its ability to promote wound healing. The hydrogels with seven different Gel and KC ratios were prepared and characterized using rheological testing, thermal analysis, spectral analysis, micromorphology observation, and X-ray diffraction.

Polysaccharide from steamed Polygonatum sibiricum ameliorates ulcerative colitis by protecting the intestinal mucosal barrier and regulating gut microbiota.

Steamed Polygonatum sibiricum is widely applied in clinical practice for its tonic effect on gastrointestinal tract. A novel polysaccharide named PSSP-EF was extracted from the steamed roots of Polygonatum sibiricum using hot water extraction, ethanol precipitation, and chromatographic purification. PSSP-EF, with a molecular weight of 2.

Multifunctional composite films with regenerated cellulose prepared via acid-catalytic degradation for in-situ growth of ZnO.

Regenerated cellulose is extensively utilized as a natural polymer due to its actually natural piezoelectric properties as well as renewable properties, but suffers from processing difficulties and low piezoelectric constants (d). Consequently, this work focuses on controlling the molecular weight of regenerated cellulose through pretreatment methods that promote the growth of in situ ZnO to enhance its d. Firstly, the acid-catalyzed pulp fibers (PF) and zinc nitrate hexahydrate were added in NaOH/urea solvent to effectively prepare RC/ZnO composite film via regeneration and in-situ growth.

CSSEC: An adaptive approach integrating consensus and specific self-expressive coefficients for multi-omics cancer subtyping.

Cancer is a complex and heterogeneous disease, and accurate cancer subtyping can significantly improve patient survival rates. The complexity of cancer spans multiple omics levels, and analyzing multi-omics data for cancer subtyping is a focus of considerable attention. However, extracting complementary information from different omics data sources and adaptively integrating them remains a major challenge.

Global prevalence of fatigue in patients with chronic obstructive pulmonary disease: a systematic review and meta-analysis.

Background: Fatigue is a common symptom in patients with chronic obstructive pulmonary disease (COPD). Published studies of fatigue among patients with COPD have presented diverse findings that may reflect variations in research methods as well as actual population differences.

Objective: To estimate the worldwide prevalence of fatigue in patients with COPD and its associated epidemiological characteristics.

Interrupting sitting with moderate-intensity physical activity breaks improves cognitive processing speed in adults with overweight and obesity: Findings from the SITLess pilot randomized crossover trial.

Introduction: Prolonged sitting can acutely reduce working memory (WM) in individuals with overweight and obesity (OW/OB) who show executive function deficits. Interrupting prolonged sitting with brief PA bouts may counter these effects. However, the benefits of such interventions on behavioral and neuroelectric indices of WM and whether neurocognitive responses are associated with postprandial glycemic responses in young and middle-aged adults with OW/OB remain unknown.

Association between Executive Function and Food Addiction among Children and Adolescents with Attention Deficit Hyperactivity Disorder.

Objectives: To examine the prevalence of food addiction (FA) among children and adolescents with attention deficit hyperactivity disorder (ADHD), and to explore the association between executive function and FA.

Study Design: This is a cross-sectional study. A total of 1,021 children and adolescents aged 6-18 years and their parents participated in this study and completed the Vanderbilt ADHD Diagnostic Parent Rating Scale, Yale Food Addiction Scale and Executive Function Behavior Rating Scale.

Ability of the modified NUTRIC score to predict all-cause mortality among ICU patients with acute pancreatitis: A retrospective analysis using the MIMIC-IV database.

Background: Acute pancreatitis (AP) is a common acute abdominal condition that can lead to severe complications. Malnutrition significantly impacts the prognosis of patients with AP, so effective tools are needed to identify those at high nutritional risk. This study validated the ability of the modified NUTRIC score to predict all-cause mortality and identify nutritional risk in patients with acute pancreatitis in the ICU.

Associations of dietary factors with amyotrophic lateral sclerosis: A Mendelian randomization study.

Background: An inconsistent yet notable relationship between dietary habits and the risk of amyotrophic lateral sclerosis (ALS) has been previously established, with the causative nature of this relationship remaining uncertain. This study aims to explore the causal connections at a genetic level.

Methods: A two-sample Mendelian Randomization (MR) based analysis was conducted utilizing a comprehensive, publicly assessable Genome-wide association study (GWAS) database.

Development and testing the psychometric properties of 20 bolt-on items for the EQ-5D-5L across 31 rare diseases.

Objective: Our objective was to develop and assess the psychometric properties of relevant bolt-on items for the EQ-5D-5L in patients with rare diseases (RDs).

Methods: Nineteen new EQ-5D-5L bolt-ons were developed based on literature review, expert input and qualitative interviews and focus groups with patients, caregivers and representatives of patient associations. A nationwide, cross-sectional, web-based survey in China included patients or caregivers of patients with 31 RDs in China (n=9,190).

Robot-assisted Endodontic Retreatment: A Case Report with Clinical Considerations.

Introduction: Fiber posts present significant challenges for nonsurgical endodontic retreatment, as improper removal may result in iatrogenic root perforation or even root fracture. Recently, robotic technology has attracted considerable attention in dentistry and active dental robotic (ADR) systems can perform procedures based on preset instructions, minimizing reliance on the dentist's experience. This case report describes the application of an ADR system for fiber post removal through an existing zirconia crown.

Lobopaucinoids A-Q, bioactive terpenoids from the soft coral Lobophytum pauciflorum collected in the South China Sea.

Two undescribed rearranged diterpenoids, lobopaucinoids A and B (1 and 2), along with thirteen undescribed lobane-type diterpenoids lobopaucinoids C-O (3-15) including a C (11) and two C (12 and 13) undescribed norditerpenoids, were isolated from the soft coral Lobophytum pauciflorum Ehrenberg (Sarcophytidae family) collected from Xisha Islands of the South China Sea. Additionally, two undescribed prenyleudesmane-type diterpenoids, lobopaucinoids P and Q (16 and 17), as well as two known lobane diterpenoids (18 and 19), were also obtained. Their structures were elucidated based on comprehensive spectroscopic data, Mosher's method, Mo(OAc) or Rh(OCOCF)-induced circular dichroism experiment, quantum chemical calculations, and single-crystal X-ray diffraction and literature comparison.

Stimuli-responsive nanoscale drug delivery system for epilepsy theranostics.

Epilepsy is a common neurological disease characterized by distinct pathological changes in the epileptogenic zone. Antiseizure drugs (ASDs) are widely used as the primary treatment for epilepsy. To improve the efficiency of ASDs medication, stimuli-responsive nanoscale drug delivery systems (nanoDDSs), triggered by either endogenous or exogenous factors, have been developed and been considered as a noninvasive and spatial-temporal approach to epilepsy theranostics.