Mechanism of Action of microRNA-146a on Lung Cancer Cells through Notch1/Hes-1 Signaling Pathway.

Objective: We explored the mechanism of action of microRNA-146a in the lung cancer cell line A549 through the Noth1/Hes-1 signal pathway.

Methods: A549 cells were divided into a control NT group without transfection, NC group with transfection of miR-146a-NC, and MM group with transfection of miR-146a mimics. We employed qRT-PCR, MTT, Hoechst33258 fluorescence staining, Transwell assay, cell wound scratch assay, and Western blot for detection of the expression of miR-146a and Notch1/Hes-1, cell activity, apoptotic capacity, and invasion and migration abilities.

The Consistency of Plasma Catecholamines and Metanephrines Analysis in China: A Comparison of Three Mainstream Commercially Available Liquid Chromatography Tandem Mass Spectrometry Kit Methods.

Objective: The consistency of plasma catecholamines and metanephrines measurements is crucial for the screening and diagnosis in pheochromocytomas and paragangliomas (PPGLs). There are many commercially available kits for plasma catecholamines and metanephrines testing, but their performance and consistency information are unknown. This study evaluated the performance of three mainstream commercially available LC-MS/MS kit methods and evaluated their consistency.

Protective Effect of Jiawei Shuxin Tang on Myocardial Ischemia-Reperfusion in Rats with Qi Deficiency and Blood Stasis and Its Correlation with NRG-1.

Objective: To investigate the protective effect of Jiawei Shuxin Tang (JWSXT) on myocardial ischemia-reperfusion injury (MIRI) rats with Qi deficiency and blood stasis and its correlation with neuregulin-1 (NRG-1).

Methods: 30 rats were used to establish the model of Qi deficiency and blood stasis, and were randomly divided into control group, MIRI model group, and JWSXT group. The traditional Chinese medicine (TCM) phenotypes, echocardiographic performance, myocardial reperfusion area, myocardial infarction area, HE staining, and NRG-1 content changes of rats in each group were compared one week after treatment.

miRNA-19a-3p Exacerbates Tube Formation by Targeting SOCS1 to Regulate JAK2/STAT3/VEGF in Diabetic Retinopathy.

Objective: Diabetic retinopathy (DR) is a retinal microangiopathy caused by diabetes mellitus. miRNAs have been shown to be involved in DR-associated micro vessel formation, influence the DR progression, and may be a therapeutic strategy for DR. miRNA-19a-3p is highly expressed in the vitreous of DR patients, however, its mechanism in the occurrence and development of DR is unclear.

Adipose Mesenchymal Stem Cell Exosomes Regulate Ferroptosis via ATF3 to Attenuate Acute Myocardial Infarction.

Objective: During acute myocardial infarction (AMI), ferroptosis occurs in cardiomyocytes, leading to ventricular remodeling. To investigate the influence of exosomes (Exo) derived from adipose mesenchymal stem cells (AD-MSCs) on cardiomyocytes, a therapeutic approach to resolve AMI may be discovered.

Methods: The Exo of AD-MSCs was isolated and identified, and internalized into cardiomyocytes.

Exploring the mechanism of α-Bisabolol in the treatment of psoriasis based on network pharmacology and experimental validation.

This study aims to evaluate the effects of α-Bisabolol in the treatment of psoriasis both in vivo and in vitro, and to elucidate its mechanism of action. We used network pharmacology to explore the potential active components and targets of α-Bisabolol in the treatment of psoriasis. Psoriasis-like models were induced in mice or keratinocytes using imiquimod (IMQ) or tumor necrosis factor-α (TNF-α).

Liposome-loaded miR-34c-5p attenuates apoptosis and oxidative stress following hypoxia-ischemia brain damage in neonatal mice by targeting Arhgap26.

Neonatal hypoxia-ischemia (HI) brain injury is considered a major cause of neonatal mortality and chronic disease morbidity worldwide. Despite its clinical importance, therapeutic options for HI injury remain limited. Here we demonstrated that miR-34c-5p expression peaks at postnatal day 10 in mice.

Multi-omics integration and machine learning identify and validate neutrophil extracellular trap-associated gene signatures in chronic rhinosinusitis with nasal polyps.

This study aimed to explore the molecular characteristics of neutrophil extracellular traps (NETs) in chronic rhinosinusitis with nasal polyps (CRSwNP). Differentially expressed gene analysis, weighted gene co-expression network analysis, and machine learning algorithms identified three core NETs-associated genes: CXCR4, CYBB, and PTAFR, which were significantly upregulated in CRSwNP patients. The diagnostic performance of these genes was evaluated using receiver operating characteristic (ROC) curves, and their clinical relevance was validated using multicenter data.

Embedding polydopamine in keratin-based biohydrogels for efficient dye removal.

Keratin-based hydrogels have shown promise as materials for wastewater remediation, yet their low surface area and poor mechanical performance hindered the practical application. To address these challenges, we developed a bowl-shaped polydopamine-loaded sponge bio-hydrogel (BPSH) with a hierarchical porous structure and high stability. The extruded keratin chains facilitated the formation of three distinct macroporous frameworks, while embedding bowl-shaped polydopamine into the bio-hydrogel significantly enhanced the hierarchical porous structure.

Improvement of interfacial, antioxidant, and emulsifying properties of pectin by grafting surfactin.

The changes in structural, interfacial, antioxidant, and emulsifying properties of pectin after grafting surfactin were investigated. Surfactin-pectin conjugate exhibited the highest surface hydrophobicity, approximately 130-fold of surfactin and 1.4-fold of pectin, accompanied by changed FTIR spectra.

Intelligent packaging film using watermelon peel pectin and betacyanins with UiO-66 as the gas adsorption system for monitoring the freshness of chilled pork.

The demand for reliable freshness monitoring technologies is growing in the food industry to enhance food safety and reduce waste. To address this need, this study incorporated varying concentrations of UiO-66 (0.0 %, 0.

Cutting-edge innovations in lignin-based nanoparticles: A review of synthesis techniques, characterization, and diverse applications.

Lignin is the most prevalent by-product of the pulp and paper industry and the world's second most abundant naturally occurring renewable biopolymer, after cellulose. Among various lignin-based materials, lignin nanoparticles (LNPs) are inexpensive and easily biodegradable by fungi and bacteria in nature, considered as a valuable class of renewable resources. Additionally, LNPs are not biotoxic and do not cause tissue rejection.

Phytic acid-modified carboxymethyl cellulose-based hydrogels for efficient removal of methylene blue dye.

With the rapid development of science and technology, the discharge of industrial wastewater, especially dye wastewater, has caused great harm to the ecological environment. In response to the pollution caused by cationic organic dyes in industrial wastewater, this study employed free radical polymerization to prepare a new hydrogel adsorbent, PAA-PA-CMC, which exhibits both reusability and a large adsorption capacity. Phytic acid (PA) and carboxymethyl cellulose (CMC) were introduced into the acrylic acid-based polymer network using N, N '-methylene-bisacrylamide (MBA) and glycidyl methacrylate (GMA) as cross-linking agents.

Broad complex negatively regulates Fibrohexamerin/P25 by binding to the cis-element BMFA in the silkworm, Bombyx mori.

Silk proteins, as natural macromolecular substances, hold significant potential for applications in biomaterials and biomedical fields. The expression of silk protein genes exhibits spatiotemporal specificity. Broad Complex (BrC), a key primary response factor to 20-hydroxyecdysone, plays a crucial role in metamorphosis.

Integrated analysis of multiple programmed cell death-related prognostic genes and functional validation of apoptosis-related genes in osteosarcoma.

Osteosarcoma (OS) is one of the most prevalent bone malignancies with a poor prognosis. Various types of programmed cell death patterns can influence cancer progression and response to treatment. We aimed to integrate different molecular characteristics of cell death for risk stratification and personalized therapy.

Silk fibroin methacryloyl hydrogel loaded with silver-gallic acid nanoparticles for enhanced diabetic wound healing.

Diabetic wound healing is hindered by oxidative stress, impaired angiogenesis, and inflammation. To address these issues, we developed a novel silver-gallic acid nanoparticle and incorporated it into a methacryloyl silk fibroin hydrogel (Ag@GA/Gel) based on the concept of polyphenol-metal nanoparticle networks for diabetic wound healing. In vitro experiments demonstrated that this hydrogel could promote macrophage polarization toward the M2 phenotype, scavenge reactive oxygen species, and exhibit pro-angiogenic and antibacterial properties.

The reduction effect on sensitization of sesame protein Ses i 3 of ultrasound-assisted glycation treatment through modulation of T cell differentiation.

This study aimed to investigate the potential reduction on the sensitization of sesame protein Ses i 3 through ultrasound-assisted glycation. Ses i 3 was extracted and purified using an immunoaffinity column, and the allergenicity changes of Ses i 3 were assessed by a comprehensive strategy, and T cell polarization was also assessed in vivo. Results showed ultrasound-assisted glycation treated Ses i 3 was more easily digestible; and the cell degranulation model showed the histamine, tryptase, and β-hexosaminidase induced by ultrasound-assisted glycation treatment were significantly decreased; besides, the serological results demonstrated that a notable decrease in the binding ability of immunoglobulin E (IgE) and IgG; finally, a BALB/c mice model demonstrated an alleviation of allergic responses induced by ultrasonic-assisted glycation treatment.

Preparation, characterization and slow-release behavior during in vitro digestion of rice porous starch-based microencapsulated camellia oil.

Microcapsules laden with camellia oil (CO), utilizing rice porous starch (PS) as the core material carrier, were successfully prepared through spray drying, employing whey protein isolate (WPI) and maltodextrin (MD) as composite wall materials. This study delved into the rheological characteristics, zeta potential, and physical stability of the CO emulsions. The results indicated a notable reduction in the apparent viscosity of the CO emulsions upon the incorporation of MD.

Mussel-inspired modification of bamboo fibers via co-deposition of polydopamine cross-linked polyethyleneimine for advanced interfacial compatibility improvement in PLA composites.

Interfacial modification has been extensively studied in the realm of plant fiber-reinforced bio-composites, with biomimetic polydopamine (PDA) modification garnering significant attention in recent years. This paper presents a novel strategy involving the one-pot co-deposition of PDA cross-linked with polyethyleneimine (PEI) to modify bamboo fibers (BF) for reinforcing polylactic acid (PLA). This approach enhances the interfacial compatibility between BF and PLA through robust linkages in a convenient, green, and non-destructive way.

Facile fabrication of transparent and stretchable cellulose ionic gel paper for sustainable use in multifunctional sensors and optoelectronic devices.

Although transparent paper derived from cellulose has been successfully demonstrated as an inexpensive, renewable and biodegradable substrate used for flexible electronics, the inherently stiff characteristic and intrinsic poor conductivity of the cellulose paper inevitably hinders its application in stretchable electronic devices. Herein, we report a new avenue for construction of highly stretchable, transparent, and ionic conductive cellulose gel paper via glycerol inducing plasticizing and CaCl initiating chelating, a facile casting and drying strategy. The renewable carboxymethyl cellulose is employed for its intrinsically abundant carboxyl groups for crosslinking with Ca via ionic coordination bonds, benefiting the improvement of various performances.

High-solids gel properties of acid-hydrolyzed waxy maize starch are determined by the molecular-weight distribution.

This study aimed to understand the effect of molecular-weight distribution on the properties of acid-hydrolyzed waxy maize starches (AH-WMSs) prepared at different acid concentrations and hydrolysis times at 50 °C. A reduction in molecular weight of AH-WMS resulted in increased paste fluidity and light transmittance, as well as decreased sedimentation, amylose‑iodine complex, and pasting viscosity. Freshly prepared AH-WMS viscoelastic gels at 20 % solids showed thixotropy at 25 °C and 50 °C, and both the shear stress and the area of hysteresis loop decreased with decreasing molecular weights.

Regulatory effects of different spacer alkyl chains on the in vitro antioxidant and antibacterial activities of chitosan quaternary ammonium/phosphonium salt derivatives.

In this study, three chitosan quaternary phosphonium salts and three chitosan quaternary ammonium salts with varying spacer chain lengths were synthesized. Structure characterization of the obtained products was conducted using Fourier-transform infrared spectroscopy, nuclear magnetic resonance, thermogravimetric analysis, and elemental analysis. Additionally, the in vitro scavenging efficiency of these derivatives against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals, as well as their antibacterial activities against Escherichia coli (E.