Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroID.

Extracellular proteins play pivotal roles in both intracellular signaling and intercellular communications in health and disease. While recent advancements in proximity labeling (PL) methods, such as peroxidase- and photocatalyst-based approaches, have facilitated the resolution of extracellular proteomes, their in vivo compatibility remains limited. Here, we report TyroID, an in vivo-compatible PL method for the unbiased mapping of extracellular proteins with high spatiotemporal resolution.

CKMT1 deficiency contributes to mitochondrial dysfunction and promotes intestinal epithelial cell apoptosis via reverse electron transfer-derived ROS in colitis.

Mitochondrial dysfunction contributes to the pathogenesis of ulcerative colitis (UC). As a mitochondrial isozyme of creatine kinases, which control energy metabolism, CKMT1 is thought to be a critical molecule in biological processes. However, the specific role of CKMT1 in intestinal inflammation remains largely unknown.

Core N-DRC components play a crucial role in embryonic development and postnatal organ development.

Motile cilia and flagella are evolutionarily conserved organelles, and their defects cause primary ciliary dyskinesia (PCD), a disorder characterized by systemic organ dysfunction. The nexin-dynein regulatory complex (N-DRC) is a crucial structural component of motile cilia and flagella, present across various species from Chlamydomonas to humans. Defects in N-DRC components lead to multiple PCD symptoms, including sinusitis and male infertility.

Biosilicification-mimicking chiral nanostructures for targeted treatment of inflammatory bowel disease.

The cascade reaction of lipopolysaccharides (LPS), cell-free DNA (cfDNA), and reactive oxygen species (ROS), drives the development of inflammatory bowel disease (IBD). Herein, we construct polyethylenimide (PEI)-L/D-tartaric acid (L/D-TA) complexes templated mesoporous organosilica nanoparticles (MON) (PEI-L/D-TA@MON) by mimicking biosilicification under ambient conditions within seconds. The chiral nanomedicines include four functional moieties, wherein PEI electrostatically attracts cfDNA, tetrathulfide bonds reductively react with ROS, silanol groups adsorb LPS, and L/D-TA enables chiral recognition and inflammatory localization.

No causal link between smoking and dementia with Lewy bodies.

This letter addresses the findings of Goodheart and Gomperts (2024), which observed an association between smoking and reduced odds of dementia with Lewy bodies (DLB). In contrast, our Mendelian randomization analysis found no causal link, suggesting that further research using genetically informed methods is needed to clarify these results.

Pushing the frontiers of babesiosis research: in vitro culture and gene editing.

Babesiosis is a tick-borne parasitic disease that poses a significant risk to both animal and human health. A comprehensive understanding of Babesia biology necessitates the application of advanced laboratory techniques. This review explores recent advancements in gene editing technologies of Babesia, emphasizing the foundational importance of in vitro culture systems.

Fertility attitudes and intentions among postgraduate female and male students in China: A qualitative focus group study.

Objective: To explore the fertility attitudes and intentions of postgraduate female and male students in China.

Methods: A qualitative descriptive study was conducted using focus group interviews and purposive sampling to select 27 postgraduate students. This diverse group included eight nursing, five pharmacy, four rehabilitation, four ophthalmology, and six stomatology students from a graduate school in China.

Aspartate aminotransferase-to-platelet ratio index as a novel predictor of early mortality in heat stroke patients: a multi-centre retrospective study.

Background: The aspartate aminotransferase-to-platelet ratio index (APRI) is an effective non-invasive marker for chronic liver dysfunction. Given that heat stroke patients often suffer from poor prognosis due to multi-organ involvement, with liver injury and coagulation dysfunction being of particular concern, this study aims to investigate whether APRI can comprehensively reflect liver injury and coagulation dysfunction in heat stroke patients and explore its relationship with 28-day mortality.

Methods: This retrospective study analysed electronic medical records from patients treated at 57 grade A tertiary hospitals in China from May 2005 to May 2024.

Association between cardiac cachexia and adverse outcomes in patients with heart failure: a meta-analysis of cohort studies.

Background: Cardiac cachexia is a condition characterised by unintentional weight loss and muscle wasting in patients with heart failure. However, there is debate about the prognostic value of cardiac cachexia in these patients.

Objectives: This meta-analysis aimed to evaluate the prognostic value of cardiac cachexia in patients who had heart failure.

Influence of depression trajectories in heart failure patients with preserved ejection fractions: a secondary analysis of adverse outcomes in the TOPCAT trial.

Background: Long-term patterns of depressive symptoms among patients with heart failure, specifically those with a preserved ejection fraction (HFpEF), and their relationship with prognoses are not well studied.

Methods: This analysis included 609 participants from the TOPCAT (Treatment of Preserved Cardiac Function Heart Failure with an Aldosterone Antagonist) trial. Depressive symptoms were assessed using the Patient Health Questionnaire-9 (PHQ-9) at baseline and at 1-year, 2-year and 3-year intervals.

Expression Profiles and Diagnostic Value of Serum Bile Acids in Intrahepatic Cholestasis of Pregnancy.

Objective: This study aimed to ascertain serum bile acid levels and explore the diagnostic value of differential bile acids in patients with intrahepatic cholestasis of pregnancy (ICP).

Methods: 94 cases of ICP patients admitted to Wenzhou People's Hospital from Oct 2020 to July 2023 were selected as the ICP group, and 184 women who underwent normal prenatal examination in the same period as the healthy control group. The baseline data and 15 kinds of serum bile acid spectrum characteristics of the two groups of pregnant women were compared, and the risk factors of serum bile acid spectrum causing ICP were analyzed by univariate and multivariate logistic regression.

Effect of miR-190a-3P on Chronic Diabetic Nephropathy by Regulating FGFR3.

Objective: Chronic diabetic nephropathy (CDN) is one of the common complications of the chronic diabetes. The FGF23/FGFR3-mediated signaling pathway is involved in CDN. Whether miR-190a-3P participates in CDN through regulation of FGFR3 remains to be elucidated.

Causal Relationships Between Inflammatory Cytokines and Sepsis: A Mendelian Randomization Study.

Objective: The complex interplay between inflammatory cytokines and sepsis is not well understood. This study employs Mendelian Randomization (MR) to investigate the causal relationships between various inflammatory cytokines and sepsis, aiming to elucidate the underlying mechanisms and potential therapeutic targets.

Methods: This study employed a bidirectional MR approach to investigate the causal effects of inflammatory cytokines on sepsis and vice versa.

Evaluation Value of Preoperative Serum Transaminase Ratio Combined with Serum Tumor Markers for Prognosis of Patients with Stage III Colorectal Cancer: A Retrospective Study.

Objective: This study utilized data from two hospitals to investigate the relationship between the preoperative serum alanine aminotransferase to aspartate aminotransferase ratio (LSR) combined with preoperative serum tumor markers (TM) and the prognosis of patients with stage III colorectal cancer (CRC).

Methods: An analysis was performed on the clinical data of 211 patients diagnosed with stage III colorectal cancer (CRC), treated surgically at the Department of Gastrointestinal Surgery of the Second Affiliated Hospital of Nanchang University and the General Surgery Department of the First Affiliated Hospital of Nanchang University between January 2016 and December 2016. Laboratory data were also compiled for these patients.

MGLL Promotes Pancreatic Cancer Progression and Invasion: Potential Therapeutic Inhibition by Melatonin.

Objective: Pancreatic adenocarcinoma (PAAD) is an aggressive and lethal cancer with limited treatment options and poor prognosis. Monoacylglycerol lipase (MGLL), a key enzyme in lipid metabolism, is thought to play a role in tumor progression, but its function in PAAD remains unclear. This study aims to investigate the expression and functional significance of MGLL in pancreatic cancer and evaluate melatonin (MLT) as a potential inhibitor of MGLL.

The IL-6/STAT3 Signaling Pathway Is Involved in Radiotherapy-Mediated Upregulation of PD-L1 in Esophageal Cancer.

Objective: This study aimed to investigate the mechanism whereby ionizing radiation improves anti-programmed cell death protein 1 (PD-1)/programmed cell death 1 ligand 1 (PD-L1) immunotherapy for esophageal squamous cell carcinoma (ESCC).

Methods: The protein levels of interleukin 6 (IL-6), signal transducer, and activator of transcription 3 (STAT3), and PD-L1 in human ESCC specimens and histologically normal tissues were analyzed by western blot. The effects of radiation exposure on PD-L1, STAT3, and IL-6 were determined in three esophageal cancer (EC) cell lines.