Metal-organic framework-based tunable platform for the immobilization of lipase with enhanced activity in non-aqueous systems.

Nowadays, metal-organic frameworks (MOFs) have been emerged as an efficient platform for enzyme immobilization due to their high porosity, tunability, and chemical versatility. In this study, a series of hybrid lipase@NKMOF-101-M (M = Mg, Mn, Zn, Co, or Ni) biocatalysts were constructed through a facile in situ encapsulation method, and the encapsulation and immobilization of lipase in MOFs were carefully validated. The catalytic activity of lipase@NKMOF-101-Mn was 2-fold higher than that of lipase@ZIF-8 and 3-fold higher than that of lipase@MCM-41 due to its excellent dispersibility and hydrophobicity in hexane.

Urchin-like magnetic nanoparticles loaded with type X collagen siRNA and Stattic to treat triple negative breast cancer under rotating magnetic field like an "enchanted micro-scalpel".

Magnetic nanoparticles effectively target drug delivery, contrast agents, biosensors, and more. Urchin-like magnetic nanoparticles (UMN) with abundant spike-like structures exhibit superior magneto-mechanical force to destroy tumor cells compared with other shapes of magnetic nanoparticles. However, when cell contents are released from tumor cells induced by magneto-mechanical force, they can act on surrounding tumor cells to facilitate tumor development.

Certain soil fungi impact the biosynthesis of amylose in maize when manure is used instead of chemical fertilizer at equal levels of nitrogen.

The protracted and immoderate utilization of chemical fertilizers has been detrimental to the composition of fungi in the soil and quality of crops. To ameliorate the adverse effects, a 6-year positioning experiment was undertaken to investigate the impact of substituting 0 % (CF), 25 % (M25), 50 % (M50), 75 % (M75), and 100 % (M100) of 225 kg ha chemical fertilizer nitrogen with manure nitrogen on both soil fungi and maize quality. This study showed that the expansion of Aspergillus heterocaryoticus, Xerochrysium dermatitidis, and Aspergillus penicillioides contributed to heightened levels of amylose and soluble sugars.

Curcumin encapsulation in self-assembled nanoparticles based on amphiphilic stearic acid-grafted inulin: Preparation, characterization, and functional evaluation.

The clinical application of curcumin (CUR) is restricted by its low solubility, instability, and poor bioavailability. To overcome these limitations, we developed a novel stearic acid-grafted inulin-based nano-delivery system for CUR encapsulation. The structure of stearoyl inulin (SA-IN) was characterized using Fourier-transform infrared spectroscopy, hydrogen nuclear magnetic resonance, thermogravimetric analysis, and contact angle measurements.

Endoplasmic reticulum stress in liver fibrosis: Mechanisms and therapeutic potential.

This paper reviews the important role of endoplasmic reticulum stress in the patho mechanism of liver fibrosis and its potential as a potential target for the treatment of liver fibrosis. Liver fibrosis is the result of sustained inflammation and injury to the liver due to a variety of factors, triggering excessive deposition of extracellular matrix and fibrous scar formation, which in turn leads to loss of liver function and a variety of related complications. Endoplasmic reticulum stress is one of the characteristics of chronic liver disease and is closely related to the pathological process of chronic liver disease, including alcohol-related liver disease, viral hepatitis, and liver fibrosis.

Hypoxia-regulated miR-103-3p/FGF2 axis in adipose-derived stem cells promotes angiogenesis by vascular endothelial cells during ischemic tissue repair.

Background: Identifying factors mediating adipose-derived stem cells (ADSCs)-induced endothelial cell angiogenesis in hypoxic skin flap tissue is critical for reconstruction. While the paracrine action of VEGF by adipose-derived stem cells (ADSCs) is established in promoting endothelial cell angiogenesis, the role of FGF2 and its regulatory mechanisms in ADSCs paracrine secretion remains unclear.

Methods: We induced hypoxia and examined the expression level of FGF2 in ADSCs using ELISA, qRT-PCR, and western blotting.

Experiences of women of childbearing age with diabetes skin pruritus: An interpretative phenomenological analysis.

Aim: This study aims to explore the experiences and feelings of women of childbearing age with diabetes skin pruritus after being diagnosed.

Methods: Using the Interpretative Phenomenological Analysis method, semi-structured interviews were conducted with women of childbearing age who suffer from diabetes skin pruritus, followed by data analysis.

Results: Three superordinate themes and eight subthemes were identified, including psychological distress (self-identity, lack of understanding from others, emotional damage and self-disgust), physical impairment (loss of bodily control, treatment challenges, impact on female sexual characteristics), and coping post-trauma (social withdrawal, active coping).

Impact of prolonged versus intermittent infusion of meropenem on mortality and clinical outcomes in patients with severe infection: A systematic review and meta-analysis.

Objectives: To compare the clinical outcomes of patients with severe infection treated with prolonged or intermittent infusion of meropenem.

Methods: PubMed, Embase, and Cochrane Central databases were searched until July 2023. Randomized controlled trials (RCTs) or observational studies comparing prolonged versus intermittent infusion of meropenem were considered eligible.

Sensitive Months for Green Spaces' Impact on Macrosomia and Interaction with Air Pollutants: A Birth Cohort Study.

Macrosomia poses significant health risks to mother and fetuses, yet the protective sensitive window for the effects of green space resources on the risk of macrosomia remains unexplored. This study identified sensitive windows of green space exposure and examined the interactions with air pollutants. In a study of 221,380 full-term newborns delivered at the Hospital, from 2017 to 2021, Normalized Difference Vegetation Index (NDVI) and atmospheric pollutant concentrations were matched to participants based on their residences in the Ningxia region.

Hidden Threat in Turbid Waters: Quantifying and Modeling the Bioaccumulation and Risks of Particulate Metals to Clams.

A major proportion of metal contaminants in aquatic environments is bound to suspended particulate matter (SPM), yet environmental monitoring typically focuses on dissolved metals, with the filtration step removing SPM. This step may inadvertently hide the potential risks posed by particulate metals. In this study, we used stable isotope tracers to quantify the contributions of SPM-bound metals to the bioaccumulation of nickel (Ni), copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) in Ruditapes philippinarum, a widely distributed clam crucial to global aquaculture.

Investigating the factors contributing to the maintenance of high levels of warm season nocturnal ozone in a basin city.

In recent years, ozone (O) pollution in many Chinese cities has worsened. Several cities have also experienced incidents where nocturnal O concentrations did not decrease as expected, and instead remained at high levels (above 50 ppb). However, there have been few detailed studies on the causes of these events.

Phases partitioning and occurrence forms of arsenic, chromium, and vanadium in a tidal reach of the Pearl River estuary, South China.

Migration characteristics and occurrence forms of redox-sensitive metal(loid)s such as arsenic (As), chromium (Cr), and vanadium (V) remained unclear in dynamic estuarine waters. In this work, size fractionation and chemical speciation of As, Cr, and V in the Jiaomen Waterway (JMW), a tidal river of the Pearl River estuary, were explored based on (ultra)filtration, the diffusive gradients in thin films (DGT) techniques and a thermodynamic chemical equilibrium model. The results showed that As was present mainly in soluble forms in the river water, and the suspended particulate matter (SPM) was identified the major carrier for Cr.

Insights into the role of dissolved organic matter derived from paddy soils with different parent materials in the environmental behavior of heavy metal adsorbed by ferrihydrite.

The interaction between dissolved organic matter (DOM) and ferrihydrite (Fh) is a crucial process to control the environmental behavior of heavy metals (HMs) in soil environments, with DOM playing a particularly strong role in HMs fate. Since chemical properties of DOM vary based on different soil parent materials, the underlying impact of DOM-Fh associations on HMs binding remains unclear. This study systematically investigated the interactions between DOM from three soil parent materials (fluvial alluvium: FDOM, sand-shale: SDOM and granite: GDOM) and Fh, and meanwhile understand their effects on the environmental behavior of Cd and Pb under various environmental conditions.

Autophagy-Targeted Therapy for Pulmonary Inflammation by 2D MX (M = W, Nb; X = S, Se) Nanosheets.

For biomedical applications, two-dimensional transition metal dichalcogenides (2D TMDCs) are often combined with other elements or functionalized with specific surface ligands, while their intrinsic biological activities are not yet fully understood. This study investigates the anti-inflammatory potential of four unmodified 2D TMDCs, including WS, WSe, NbS, and NbSe nanosheets, in LPS-activated MH-S cells in vitro and in a mouse model of pulmonary inflammation in vivo. Despite their varying compositions, these 2D TMDCs exhibited comparable anti-inflammatory effects in LPS-activated MH-S cells.

Iron oleate containing lipid nanoparticles prepared by gradient solvent diffusion method for oxidative stress dependent antibacterial therapy.

This study was designed to assess the efficacy of iron oleate lipid nanoparticles (IO-LNPs) in inducing Fenton reaction as a therapeutic approach for bacterial infections caused by Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), both of which are common pathogens in skin wound infections.

Co-delivery of minoxidil and finasteride via ionic liquid and cyclodextrin-assisted in situ thermosensitive hydrogel for synergistic treatment of androgenic alopecia.

Androgenic alopecia (AGA), the most prevalent type of progressive hair loss, currently lacks an effective topical treatment regimen. In this study, we synthesized an ionic liquid (IL) to co-solubilize minoxidil (MXD) and finasteride (FIN) and subsequently formulated them into an in situ thermosensitive ionic liquid/cyclodextrin/poloxamer hydrogel (ICPG), termed M + F@ICPG. M + F@ICPG was developed for the transdermal co-delivery of these two drugs, aiming to provide a multipath therapeutic approach for AGA while avoiding the adverse effects commonly associated with oral FIN and topical MXD tincture.

Decoding ferroptosis in alcoholic hepatitis: A bioinformatics approach to hub gene identification.

Background: Ferroptosis is associated with alcoholic hepatitis (AH); however, the underlying mechanisms remain unclear.

Methods: Changes in iron content and oxidative stress in AH patients and in vivo and in vitro models were analyzed. Iron homeostasis pathways in the livers of patients with AH were investigated using RNA sequencing.

Lysyl oxidase nanozyme-loaded hydrogel for sustained release and promotion of diabetic bone defect regeneration.

The process of regenerating bone injuries in diabetic presents significant challenges because lysine oxidase (LOX), a key catalytic enzyme for collagen cross-linking, is inhibited in hyperglycemia. The supplementation of LOX is constrained by inadequate sources and diminished enzymatic activity, necessitating the development of effective alternatives for enhancing bone regeneration in diabetes. Herein, we reported a lysyl oxidase nanozyme (LON), derived from the catalytic domain of LOX.

N-glycosylation of ephrin B1 modulates its function and confers therapeutic potential in B-cell lymphoma.

Given the pivotal role of the Eph-Ephrin signaling pathway in tumor progression, agonists or antagonists targeting Eph/Ephrin have emerged as promising anticancer strategies. However, the implications of glycosylation modifications within Eph/Ephrin and their targeted protein therapeutics remain elusive. Here, we identify that N-glycosylation within the receptor-binding domain (RBD) of ephrin B1 (EFNB1) is indispensable for its functional repertoire.

TET1 participates in oxaliplatin-induced neuropathic pain by regulating microRNA-30b/Nav1.6.

Chemotherapy-induced neuropathic pain poses significant clinical challenges and severely impacts patient quality of life. Sodium ion channels are crucial in regulating neuronal excitability and pain. Our research indicates that the microRNA-30b (miR-30b) in rat dorsal root ganglia (DRG) contributes to chemotherapy-induced neuropathic pain by regulating the Nav1.

E3 ubiquitin ligase TRIM7 alleviates LPS-induced acute lung injury via inhibiting NLRP3 inflammasome activation.

Acute lung injury (ALI) is a clinically common disease with high mortality, characterized by tissue damage caused by excessive activation of inflammation. TRIM7 is an E3 ligase that plays an important role in regulating viral infection, tumor progression and innate immune response. But its function in ALI is unclear.

Identification and regulation of a novel leptin receptor-linked enhancer during zebrafish ventricle regeneration.

Aims: Vertebrates vary greatly in their abilities to regenerate injured hearts. Zebrafish possess a remarkable capacity for cardiac regeneration, making them an excellent model for regeneration research. Recent studies have reported the activation and underlying regulatory mechanisms of leptin b (lepb) and the leptin b-linked enhancer (LEN) in injured hearts.

Research of Orthodontic Soft Tissue Profile Prediction Based on Conditional Generative Adversarial Networks.

Objective: This study constructed a new conditional generative adversarial network (CGAN) model to predict changes in lateral appearance following orthodontic treatment.

Methods: Lateral cephalometric radiographs of adult patients were obtained before (T1) and after (T2) orthodontic treatment. The expanded dataset was divided into training, validation, and test sets by random sampling in a ratio of 8:1:1.

Adaptation of maxillary removable partial denture frameworks fabricated with a direct digital workflow: A randomized crossover clinical trial.

Objectives: To compare the adaptation of maxillary removable partial denture (RPD) frameworks fabricated through direct digital workflows with that of traditional cast frameworks and indirect digital frameworks.

Methods: The workflow for fabricating the digital cobalt-chromium framework encompassed intraoral scanning (IOS) using Trios 3, computer-aided survey and design, and subsequently either the lost-wax technique from a printed resin framework pattern (Framework B) or direct selective laser melting (SLM) (Framework C). The traditional cast framework (Framework A) was selected as a control.

Ethyl 2,2-difluoro-2-(2-oxo-2H-chromen-3-yl) acetate inhibits the malignant biological behaviors of colorectal cancer by restricting the phosphorylation and nuclear translocation of STAT3.

To investigate the effect of a novel coumarin derivative, ethyl 2,2-difluoro-2 - (2-oxo-2H-chromen-3-yl) acetate (C2F), on the malignant biological behaviors of colorectal cancer (CRC) and elucidate its mechanism. In vitro, the effects of C2F on the proliferation, apoptosis, migration, invasion, and cell cycle of CRC cells were analyzed by MTT assay, EdU stainning, colony formation assay, flow cytometry, wound healing and transwell assay. The anti-CRC activity of C2F was evaluated in a nude mice xenograft model in vivo.