Isothermal nucleic acid amplification for monitoring hand-foot-and-mouth disease: current status and future implications.

With the global prevalence of the hand-foot-and-mouth disease (HFMD) epidemic, the development of reliable point-of-care testing (POCT) is crucial for the timely identification and prevention of outbreaks. Isothermal nucleic acid amplification techniques (INAATs) have attracted much attention because of their high efficiency for rapid diagnosis. In this work, we systematically summarize the current status of INAATs for HFMD and discuss advantages and drawbacks of various INAATs for HFMD.

JAK2 inactivating mutations promotes endometrial cancer progression by targeting HIF-1α.

Objective: Endometrial cancer (EC) is the ninth most common malignancy among women. While mutations in JAK2 are frequently observed in EC, the specific biological functions of JAK2 in endometrial cancer are poorly understood.

Methods: The genetic alterations of JAK2 in different cancer types were explored using sequencing dataset deposited at TCGA database.

ADAMTS12 serves as a novel prognostic biomarker and promotes proliferation and invasion in gastric cancer.

Gastric cancer (GC) remains a prevalent and aggressive malignancy with a poor prognosis. This study aimed to identify diagnostic and prognostic biomarkers while exploring their potential functions in GC. A total of 598 upregulated and 506 downregulated genes were identified in GC patients.

In-situ Growth of Metallocluster inside Heterometal-organic Cage to Switch Electron Transfer for Targeted CO2 Photoreduction.

Construction of metal-organic cages (MOCs) with internal modifications is a promising avenue to build enzyme-like cavities and unlocking the mystery of highly catalytic activity and selectivity of enzymes. However, current interests are mainly focused on single-metal-node cages, little achievement has been expended to metalloclusters-based architectures, and the in situ endogenous generation of metal clusters. Herein, based on the hard-soft-acids-bases (HSAB), the metalloclusters-based heterometallic MOC (Cu3VMOP) constructed of [Cu3OPz3]+ and [V6O6(OCH3)9(SO4)(CO2)3]2- clusters was obtained by one-pot method.

Discovery of Novel 4,5,6,7-Tetrahydro-7-pyrazolo[3,4-]pyridin-7-one Derivatives as Orally Efficacious ATX Allosteric Inhibitors for the Treatment of Pulmonary Fibrosis.

Pulmonary fibrosis (PF) is a progressive, fatal lung disease lacking effective treatments. Autotaxin (ATX) plays a crucial role in exacerbating inflammation and fibrosis, making it a promising target for fibrosis therapies. Herein, starting from PAT-409 (Cudetaxestat), a series of novel ATX inhibitors bearing 1-indole-3-carboxamide, 4,5,6,7-tetrahydro-7-pyrazolo[3,4-]pyridin-7-one, or 4,5,6,7-tetrahydro-1-pyrazolo[4,3-]pyridine cores were designed based on the structure of ATX hydrophobic tunnel.

Cuminaldehyde Potentiates Antiproteolytic Peptide Efficacy via Parallel Pathways of Enhanced Inner Membrane-Damaging Activity and Inhibition of Bacterial Energy Metabolism.

Antimicrobial peptides (AMPs) offer potential as antibiotic alternatives, but high cost, off-site cytotoxicity, and poor stability limit their application. Combining AMPs with adjuvants holds promise in surmounting these limitations. Among potentiators, terpenoids account for the highest proportion, yet their potential to enhance the AMPs efficacy and underlying mechanism remain unclear.

3D Interwoven SiC/g-CN Structure for Superior Charge Separation and CO Photoreduction Performance.

To address the limitations of carbon nitride in photocatalysis, we propose constructing a three-dimensional interwoven SiC/g-CN composite structure. Utilizing the strong microwave-thermal conversion characteristics of SiC whiskers, localized "hot spots" are generated, which induce rapid thermal gradients, promoting rapid polymerization of urea and in situ formation of the interwoven network. This unique structure strengthens the interaction between these two components, creates multiple electron transport pathways, enhances CO adsorption, and effectively improves charge separation while reducing photogenerated carrier recombination.

Palladium-Catalyzed Enantioselective Cyclization of 1,6-Enynes to Access Chiral γ-Butyrolactam.

A palladium-catalyzed asymmetric chlorocyclization of 1,6-enynes has been described. Controlling the chloride ion concentration in the system by substrate design is the key to achieving asymmetric chlorinated cyclization. In the presence of Pd(PhCN)Cl and chiral phosphoramidite ligands, the reaction accesses diverse chiral ()-α-chloromethylene-γ-butyrolactams with excellent selectivity and enantioselectivity.

Solid Solution Derived Cu Clusters on Partially Reduced CuCeO2 with Abundant Oxygen Vacancies Enable Efficient Reverse Water Gas Reaction.

The reverse water gas shift (RWGS) reaction provides a convenient approach to convert CO2 to CO, which facilitates to achieve the goals of carbon peaking and carbon neutrality. Herein, the Cu/CeO2 catalyst prepared by a co-precipitation method using a mixture of Na2CO3 and NaOH at pH of 10 (sample Cu/CeO2-10) achieved an intrinsic reaction rate of 428.4 mmol•gcat-1•h-1 with 100% CO selectivity at 400 °C and CO2/H2 ratio of 1:4, which is much higher than Cu/CeO2 prepared by impregnation and other methods.

Theranostic Rhenium(I)-Based ER-Phagy Retardant Promotes Immunogenic Cell Death.

ER-phagy is a double-edged sword in the occurrence, development, and treatment of cancer; especially, its functions in immunotherapy are still unknown. In this work, we designed a theranostic Re complex () containing a BODIPY-derived ligand and a β-carboline ligand to target the endoplasmic reticulum (ER) and block ER-phagy at the late stages. Interestingly, as validated both in vitro and in vivo, ER-phagy blockage greatly enhances the capability of to induce immunogenic cell death (ICD).

Dual-Amplification Single-Particle ICP-MS Strategy Based on Strand Displacement Amplification-CRISPR/Cas12a Amplification for Homogeneous Detection of miRNA.

MicroRNAs (miRNAs) regulate a myriad of biological processes and thus have been regarded as useful biomarkers in biomedical research and clinical diagnosis. The specific and highly sensitive detection of miRNAs is of significant importance. Herein, a sensitive and rapid dual-amplification elemental labeling single-particle inductively coupled plasma-mass spectrometry (spICP-MS) analytical method based on strand displacement amplification (SDA) and CRISPR/Cas12a was developed for miRNA-21 detection.

A Nanothermometer with a Microwave Thermal Effect for Sensing Cell Membrane Temperature and Measuring Microwave-Induced Thermal Gradient Distribution.

In microwave (MW) thermotherapy, it is challenging to regulate the temporal and spatial distribution of the temperature at the nanoscale. Herein, we report a nanothermometer for simultaneous MW heating and temperature distribution measurement. The nanothermometer was prepared by free radical polymerization with vinylbenzyl trimethylammonium chloride (VBTMACl) as the MW thermosensitizer and isopropylacrylamide (NIPAM) as the thermoresponsive unit, followed by anion exchange with fluorophore sodium 3-(4-(1,2,2-triphenylvinyl)phenoxy)propane-1-sulfonate (TPESONa).

Solar-Light-Mediated Phototransformation of Herbicide Tribenuron-Methyl Initiated by Its Coexisting Nitrate Ion in Sunlit Agricultural Drainages.

Understanding the environmental fate of chemical herbicides is crucial to sustainable agriculture. Due to their joint-use with nitrogen fertilizers, their residues often coexist with NO in agricultural drainages. In this study, tribenuron-methyl was used as a model to evaluate the role of NO in the phototransformation of chemical herbicides, which was characterized by a two-stage process.

Time-Course Strategy Reveals a Dual Potential of Perfluorooctanoic Acid and Its Alternative in Adipocyte Differentiation.

Exposure to perfluorooctanoic acid (PFOA) and hexafluoropropylene oxide dimer acid (HFPO-DA) was associated with adipogenesis. However, potential mechanisms remain to be elucidated. Herein, a 3T3-L1 adipocyte model was used to explore the dynamic changes in adipocyte differentiation (2, 4, and 8 days) under PFOA and HFPO-DA exposure.

Research progress and application status of organoid in breast cancer subtypes.

Breast cancer (BC) is a prevalent malignant tumor that poses a significant health risk to women. The complexity of basic BC research and clinical treatment is influenced by multiple factors, including age, fertility, hormone metabolism, molecular subtypes, and tumor grading and staging. Traditional in vitro models often fall short of meeting modern research demands, whereas organoids-an emerging 3D primary culture technology-offer a unique platform that better replicates the tumor microenvironment (TME).

Enhanced Water Splitting Electrocatalysis with Heterointerfacial Cobalt Phosphide In Situ Supported on a Cellulose-Derived Carbon Aerogel.

The active site density, intrinsic activity, and supporting substrate of cobalt phosphide catalysts are vital to their performance in alkaline water electrolysis. In this work, a CoP/CoP loaded on cellulose nanofiber-derived carbon aerogels (CP/CCAs) bifunctional electrocatalyst with a three-dimensional network and heterostructure is illustrated through sequential facile hydrothermal, freeze-drying, and phosphorylation processes. The three-dimensional network of carbon aerogels derived from cellulose nanofibers reveals a specific surface area of 183.

Electrochemical Reductive Bimolecular Cycloaddition of 2-Arylideneindane-1,3-diones for the Synthesis of Spirocyclopentanole Indane-1,3-diones.

An electrochemical reductive bimolecular cycloaddition of 2-arylideneindane-1,3-diones has been reported for the synthesis of spirocyclopentanole indane-1,3-diones bearing five contiguous stereocenters with vicinal tetrasubstituted stereocenters, especially involving a quaternary carbon center, in moderate to good yields and excellent diastereoselectivities. The present protocol features mild reaction conditions, no external chemical redox reagents, excellent atom economy, and gram-scale synthesis. In addition, a mechanistic investigation indicates that the reactions proceed through a radical pathway.

Switch-Type Electrochemiluminescence Aptasensor for AFB1 Detection Based on CoS Quantum Dots Encapsulated in Co-LDH and a Ferrocene Quencher.

Among the various aflatoxin B1 (AFB1) assays, performing accurate detection is difficult because false positives and false negatives are frequent due to limited sensitivity, expensive equipment, or inadequate pretreatment during operation. Here, an "off-on" switch-type electrochemiluminescence (ECL) aptasensor armed with cobalt-sulfur quantum dots was encapsulated in hollow cobalt-layered double hydroxide nanocages as an enhanced luminescent probe (Co-LDH@QDs), and a ferrocene-modified aptamer (Fc-APT) was used as a luminescent quencher. In general, when Fc-APT was hybridized with complementary DNA modified with a DNA nanotetrahedron, electron transfer between ferrocene and Co-LDH@QDs was facilitated, leading to efficient quenching of the ECL intensity into an "off" state in the absence of AFB1.

Pd/Brønsted Acid Co-catalyzed Dehydrative Coupling of Propargylic Alcohols with Diarylphosphine Oxides.

An efficient dehydrative coupling of propargylic alcohols with diarylphosphine oxides to construct tetrasubstituted allenylphosphoryl compounds in the presence of a Pd/Brønsted acid co-catalyst has been developed. As a benefit from the use of a Brønsted acid, this reaction could perform under mild conditions with excellent yields, accommodating a wide range of functional groups. The potential utility of this method has also been demonstrated.

Relation of 91 Circulating Inflammatory Proteins to Nonalcoholic Fatty Liver Disease: A Two-Sample Mendelian Randomisation Study.

Increasingly, emerging research evidence has demonstrated that nonalcoholic fatty liver disease (NAFLD) is a disease closely associated with systemic inflammation. However, the specific upstream inflammatory factors engaged in the pathogenesis of NAFLD remain unclear. Our study aimed to identify the inflammatory regulators causally associated with NAFLD pathogenesis through Mendelian randomisation.

Defect Crystal Formation and Thermal-Induced Structural Ordering of Semicrystalline Copolymers Induced by Comonomer Inclusion/Exclusion.

Comonomer defects can induce semicrystalline polymers to form unique crystalline structures (., defect crystals), which can greatly influence the materials' physical properties. However, the formation mechanism and structural evolution of defect polymer crystals are not yet well understood.

Single-Cell RNA-Seq Reveals Injuries in Aortic Dissection and Identifies PDGF Signalling Pathway as a Potential Therapeutic Target.

Aortic dissection (AD) represents a critical condition characterised by a tear in the inner lining of the aorta, leading to the leakage of blood into the layers of the aortic wall, posing a significant risk to life. However, the pathogenesis is unclear. In this study, scRNA-seq was applied to cells derived from aortas of both AD and non-AD donors (control) to unveil the cellular landscape.

Designed Synthesis of Mesoporous sp Carbon-Conjugated Benzothiadiazole Covalent Organic Frameworks for Artificial Photosynthesis of Hydrogen Peroxide.

Artificial photosynthesis of hydrogen peroxide (HO) from ambient air, water, and sunlight has attracted considerable attention recently. Despite being extremely challenging to synthesis, sp carbon-conjugated covalent organic frameworks (COFs) can be powerful and efficient materials for the photosynthesis of HO due to desirable properties. Herein, we report the designed synthesis of an sp carbon-conjugated COF, BTD-spc-COF, from benzothiadiazole and triazine units with high crystallinity and ultralarge mesopores (∼4 nm).

Application of Keystone Design Perforator Island Flap in the Reconstruction of Maxillofacial Defect: A Case Series.

To investigate the effect of Keystone Design Perforator Island Flap (KDPIF) in the repair of maxillofacial soft tissue defect. From June 2021 to June 2023, 16 patients with soft tissue defects caused by resection of maxillofacial tumor repaired by KDPIF were selected. Variants of KDPIF were designed according to the area of the defect, and the incision was parallel to the wrinkle as far as possible in the same facial cosmetic subunit.

Photochromic Film Based on a Mixed-Heteroatom-Templated Er-Incorporated Polyoxometalate Used for Inkless Prints and Anti-Counterfeiting.

Photochromic films have attracted increasing attention for their potential in information storage and photoswitchable devices. Developing novel photochromic materials is still a highly challenging topic. In this work, we successfully obtained an unprecedented mixed-heteroatom-templated Er-incorporated polyoxometalate [HN(CH)]NaKH{Er(HO)WO[SeTeWO][B-α-TeWO]}·117HO () by concurrently introducing the [TeO] and [SeO] heteroanion templates into the Er/WO system.