A Supramolecular Fluorescent Chemosensor Enabling Specific and Rapid Quantification of Norepinephrine Dynamics.

Host-guest supramolecular fluorescence probes have garnered significant attention in the detection and sensing of bioactive molecules due to their functionalization potential, adjustable physical properties, and high specificity. However, such probes that reliably, rapidly, and specifically measure neurotransmitter dynamics at the cellular and in vivo level have yet to be reported. Herein, we present a supramolecular fluorescent chemosensor designed for norepinephrine (NE) detection, showing an exceptional response and specificity through host-guest complexation.

Aptamer-Modified TiCT MXene Fluorescent Nanoprobe for Monitoring ATP and GTP during a Mild-Photothermal-Activated Nucleolar Stress Process in Living Cells.

Understanding the molecular energy metabolism of single cells in the nucleolus stress response induced by mild-photothermal therapy (mPTT) is of great importance for investigating the photothermal lethal mechanism. Herein, we successfully fabricated a "turn-on"-type fluorescent nanoprobe based on the fluorescently labeled aptamers (FAM-ATP-apt and Cy3-GTP-apt) and TiCT MXene. When the adapters on the nanoprobes bonded to intracellular ATP and GTP, the fluorescence of the nanoprobes was restored.

Substituent-Controlled Regiodivergent Rearrangement of Gramine Ammonium Ylide.

The complicated mechanism makes the regiodivergent rearrangement of ammonium ylide seem to be out of reach. Herein, we reported a regiodivergent rearrangement of gramine ammonium ylide well controlled by the substituents. Density functional theory studies reveal that the ammonium ylide with a more steric hindrance substituent 2-diazo-2-arylacetate goes through a stepwise mechanism to yield both a kinetically and thermodynamically preferred [1,2]-rearrangement product.

Carbon Dioxide Sensing Based on Off-Axis Integrated Cavity Absorption Spectroscopy Combined with the Informer and Multilayer Perceptron Models.

Off-axis integrated cavity output spectroscopy (OA-ICOS) allows the laser to be reflected multiple times inside the cavity, increasing the effective absorption path length and thus improving sensitivity. However, OA-ICOS systems are affected by various types of noise, and traditional filtering methods offer low processing efficiency and perform limited feature extraction. Deep learning models enable us to extract important features from large-scale, complex spectral data and analyze them efficiently and accurately.

Synergistic binding ability of electrostatic tweezers and femtosecond laser-structured slippery surfaces enabling unusual droplet manipulation applications.

We propose a novel contactless droplet manipulation strategy that combines electrostatic tweezers (ESTs) with lubricated slippery surfaces. Electrostatic induction causes the droplet to experience an electrostatic force, allowing it to move with the horizontal shift of the EST. Because both the EST and the slippery operating platform prepared by a femtosecond laser exhibit a strong binding effect on droplets, the EST droplet manipulation features significant flexibility, high precision, and can work under various operating conditions.

Optimized Ni(II)-doping in Co(III)-based layered double hydroxides towards electrochemical oxygen evolution catalysis.

An OER catalyst showing both high activity and stability in promoting oxygen evolution is important for its practical application in electrochemical water-splitting. Here, we report the screening of such a catalyst by optimizing the Ni(II)-doping in Co(III)-based layered double hydroxides (LDHs). Such LDH samples tailored with Ni(II)-doping are prepared by an oxidative intercalation reaction where brucite-like Ni(II)Co(II)(OH) (0 ≤ ≤ 0.

Comparison of Resilience Measures in Chinese Adolescents: Based on Item Response Theory.

Resilience is an important ability that helps adolescents resist stress from everyday life. While resilience has been measured in many ways, no measures have been developed specifically for the adolescent population. This study analyzed three popular resilience questionnaires, evaluated their psychometric properties using item response theory and explored their applicability.

Subattojoule Electrical Switching in a Two-Dimensional TaSe Oxide Device with High Endurance.

Recent developments in artificial intelligence and the internet-of-things have created great demand for low-power microelectronic devices. Two-dimensional (2D) electrical switching materials are extensively used in neuromorphic computing technology, yet their high leakage current and low endurance impede their further application. This study presents a vertical crossbar-structured conductive-bridge threshold switching device based on 2D TaSe oxide.

Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels.

Understanding the interaction between nanomaterials and cellular structures is crucial for nanoparticle applications in biomedicine. We have identified a subtype of stress granules, called nanomaterial-provoked stress granules (NSGs), induced by gold nanorods (AuNRs). These NSGs differ from traditional SGs in their physical properties and biological functions.

Investigating the role of IL-6 in the pathogenesis of systemic lupus erythematosus: Insights from bone marrow mesenchymal stem cells.

Background: Systemic lupus erythematosus is a common autoimmune disease. Studies have suggested that defective stem cells could be involved in the pathogenesis of systemic lupus erythematosus, which leads to changes in the function of immune cells. By observing the cell morphology, autophagy, and senescence of bone marrow mesenchymal stem cells (BMSCs) from lupus mice and normal controls, this study investigated the role of IL-6 in autophagy and senescence of BMSCs and explored relevant mechanisms.

Exogenous Coreactant-Free Electrocatalytic Reactive Oxygen Species-Driven Dual-Signal Molecularly Imprinted Electrochemiluminescence Sensor for the Detection of Trenbolone.

Conventional dual-signal electrochemiluminescence (ECL) sensors feature high sensitivity and reliability, but the involvement of coreactants inevitably results in a complex configuration and shows reproducibility risk. Here, we propose an exogenous coreactant-free dual-signal platform, comprising luminol (anodic luminophore), CdSe quantum dots (cathodic luminophore), and CoO/TiC electrocatalyst (coreaction promoter). At different redox potentials, CoO/TiC induces water oxidation and oxygen reduction to produce OH and O radicals, which subsequently drive cathodic and anodic ECL emission, respectively.

Neoantigen mRNA vaccines and AA receptor antagonism: A strategy to enhance T cell immunity.

Although neo-antigen mRNA vaccines are promising for personalized cancer therapy, their effectiveness is often limited by the immunosuppressive tumor microenvironment (TME). The adenosine AA receptor (AAR) inhibits dendritic cell (DC) function and weakens antitumor T cell responses through hypoxia-driven mechanisms within the TME. This review explores a novel strategy combining neo-antigen mRNA vaccines with AAR antagonists (AARi).

Volatile Resistive Switching and Short-Term Synaptic Plasticity in a Ferroelectric-Modulated SrFeO Memristor.

SrFeO (SFO) offers a topotactic phase transformation between an insulating brownmillerite SrFeO (BM-SFO) phase and a conductive perovskite SrFeO (PV-SFO) phase, making it a competitive candidate for use in resistive memory and neuromorphic computing. However, most of existing SFO-based memristors are nonvolatile devices which struggle to achieve short-term synaptic plasticity (STP). To address this issue and realize STP, we propose to leverage ferroelectric polarization to effectively draw ions across the interface so that the PV-SFO conductive filaments (CFs) can be ruptured in absence of an external field.

Solvent-controlled silver catalyzed radical transformation of α-imino-oxy acids with cyclic aldimines.

A silver-catalyzed cross coupling of cyclic aldimines and α-imino-oxy acids has been developed. The solvent-dependent reaction could selectively deliver either cyclic imine moiety retained nitriles or ring-opened oxonitriles in moderate yields. The mechanistic studies show that the reaction undergoes a radical pathway.

Temperature and light dual-responsive hydrogels for anti-inflammation and wound repair monitoring.

Wound healing is a complex and dynamic biological process that requires meticulous management to ensure optimal outcomes. Traditional wound dressings, such as gauze and bandages, although commonly used, often fall short in their frequent need for replacement, lack of real-time monitoring and absence of anti-inflammatory and antibacterial properties, which can lead to increased risk of infection and delayed healing. Here, we address these limitations by introducing an innovative hydrogel dressing, named PHDNN6, to combine wireless Bluetooth temperature monitoring and light-triggered nitric oxide (NO) release to enhance wound healing and management.

Copper(II) complexes of 2-hydroxy-1-naphthaldehyde Schiff bases: synthesis, activity and computational studies.

Background: Due to the divers biological applications of Cu(II) complexes, we in this study reports the various Cu(II) complexes. The study aims to synthesize and assess new Cu(II) complexes as powerful β-glucuronidase inhibitors.

Methods: Five Schiff base ligands and their complexes were synthesized, characterized, and screened against β-glucuronidase inhibitory activity.

Relationships between knee and ankle strength and horizontal deceleration performance at different sprint distances in multi-directional sports athletes.

The aim of this study was to investigate the relationships between knee and ankle strength and horizontal deceleration performance following different sprint distances. Fifty-seven ( = 41male,  = 16 female) youth team-sports athletes completed: a) 5-m and 10-m horizontal deceleration ability (HDA) tests; b) concentric (60°/s, 180°/s) and eccentric (30°/s) relative peak torque (PT) measurements of the knee extensor (KE) and flexor (KF) muscles and the ankle plantarflexion (APF) and dorsiflexion (ADF) muscles in an isokinetic dynamometer. Pearson's correlation coefficients revealed that concentric at 60°/s and eccentric at 30°/s KE were mostly related to deceleration performance both in HDA ( = -0.

Construction of Nanocellulose Aerogels with Environmental Drying Strategy without Organic Solvent Displacement for High-Efficiency Solar Steam Generation.

Solar desalination is one of the effective means to alleviate water scarcity, in which aerogel-like evaporators have attracted extensive attention in the field of efficient desalination. However, the current preparation methods for aerogels still mainly rely on high-cost solutions, such as freeze-drying or supercritical drying. Herein, a preparation scheme for aerogels that can be realized under atmospheric pressure conditions is reported.

Long-Term Intracranial Progression-Free Survival in Lung Cancer Brain Metastases Extended Beyond 50 Months with Gubenxiaoyi Formula (GBXY): A Groundbreaking Case Study.

Background: The prevalence of brain metastases (BM) in lung cancer patients is notably high and is associated with poor prognoses. The efficacy of standard treatment regimens in improving intracranial progression-free survival (IPFS) for lung cancer BM is markedly limited. While traditional Chinese medicine (TCM) has been effective in enhancing the quality of life and prognosis of lung cancer patients, its efficacy in treating BM remains unreported.

Mechanisms of (Fisch.) Bge. var. mongholicus (Bge.) Hsiao (huang qi) and (Oliv.) Diels (dang gui) in Ameliorating Hypoxia and Angiogenesis to Delay Pulmonary Nodule Malignant Transformation.

Screening for pulmonary nodules (PN) using low-dose CT has proven effective in reducing lung cancer (LC) mortality. However, current treatments relying on follow-up and surgical excision fail to fully address clinical needs. Pathological angiogenesis plays a pivotal role in supplying oxygen necessary for the progression of PN to LC.

Real-world Plasma Exposure of Nirmatrelvir/Ritonavir in Chinese Hospitalized Patients With COVID-19: A Multicenter Retrospective Study.

Background: Nirmatrelvir/ritonavir is licensed for the treatment of mild-to-moderate coronavirus disease (COVID-19) in patients at an increased risk of progression to severe disease. However, data on the real-world plasma exposure to nirmatrelvir/ritonavir remain limited, particularly in Chinese patients. This study aimed to assess the nirmatrelvir/ritonavir trough concentration (Ctrough) and identify its critical factors in hospitalized Chinese patients treated with nirmatrelvir/ritonavir 300 mg/100 mg twice daily over a 5-day course.

alleviates ischemia-reperfusion injury in steatotic donor liver by inhibiting ferroptosis via the Foxo3-Alox15 signaling pathway.

Ischemia-reperfusion injury (IRI) is a major obstacle in liver transplantation, especially with steatotic donor livers. Dysbiosis of the gut microbiota has been implicated in modulating IRI, and plays a pivotal role in regulating host inflammatory and immune responses, but its specific role in liver transplantation IRI remains unclear. This study explores whether can mitigate IRI and its underlying mechanisms.

Recent advances in fluorescent gold nanocluster-based bioanalytical analysis and imaging in living cell.

Ultrasmall and highly fluorescent gold nanoclusters (Au NCs) have been widely used for the construction of sensing and imaging platforms. Specifically, through a combination of surface functionalization and spectral analysis and/or imaging techniques, effective intracellular detection and imaging are realized. In this review, we summarize the recently adopted intracellular analysis and imaging events with Au NCs-based probes.

Extracellular thiol isomerase ERp5 regulates integrin αIIbβ3 activation by inhibition of fibrinogen binding.

Recent studies have shown that anti-ERp5 antibodies inhibit platelet activation and thrombus formation; Moreover, ERp5-deficient platelets exhibit enhanced platelet reactivity via regulation of endoplasmic reticulum (ER) stress. In this study, we used a new ERp5-knockout mouse model as well as recombinant ERp5 (rERp5) protein, to examine the role of ERp5 in platelet function and thrombosis. Although platelet-specific ERp5-deficient mice had decreased platelet count, the mice had shortened tail-bleeding times and enhanced platelet accumulation in FeCl-induced mesenteric artery injury, compared with wild-type mice.

Iron(II/III) Alters the Relative Roles of the Microbial Byproduct and Humic Acid during Chromium(VI) Reduction and Fixation by Soil-Dissolved Organic Matter.

Though reduction of hexavalent chromium (Cr(VI)) to Cr(III) by dissolved organic matter (DOM) is critical for the remediation of polluted soils, the effects of DOM chemodiversity and underlying mechanisms are not fully elucidated yet. Here, Cr(VI) reduction and immobilization mediated by microbial byproduct (MBP)- and humic acid (HA)-like components in (hot) water-soluble organic matter (WSOM), (H)WSOM, from four soil samples in tropical and subtropical regions of China were investigated. It demonstrates that Cr(VI) reduction capacity decreases in the order WSOM > HWSOM and MBP-enriched DOM > HA-enriched DOM due to the higher contents of low molecular weight saturated compounds and CHO molecules in the former.