Dynamic, Single-cell Monitoring of RNA Modifications Response to Viral Infection Using a Genetically Encoded Live-cell RNA Methylation Sensor.

RNA modifications, such as N6-methylation of adenosine (m6A), serve as key regulators of cellular behaviors, and are highly dynamic; however, tools for dynamic monitoring of RNA modifications in live cells are lacking. Here, we develop a genetically encoded live-cell RNA methylation sensor that can dynamically monitor RNA m6A level at single-cell resolution. The sensor senses RNA m6A in cells via affinity-induced cytoplasmic retention using a nuclear location sequence-fused m6A reader.

Theoretical Study on the Kinetics of Secondary Oxygen Addition Reactions for N-Butyl Radicals.

Chemical kinetics for second oxygen addition reactions (·QOOH + O) of long-chain alkanes are of great importance in low-temperature combustion technologies. However, kinetic data for key reactions of ·QOOH + O systems are often difficult to obtain experimentally and are primarily estimated or calculated by using theoretical methods. In this work, barrier heights (BHs), reaction energies (Δs), and relative energies (REs) of stationary points for key reactions of two representative ·QOOH + O systems in the low-temperature oxidation of -butyl as well as pressure-dependent rate constants for the involved reactions are calculated with the high-level quantum chemical method CCSD(T)-F12b/CBS.

Exploring Extracellular Vesicles: A Novel Approach in Nonalcoholic Fatty Liver Disease.

Nonalcoholic fatty liver disease (NAFLD) represents an increasing public health concern. The underlying pathophysiological mechanisms of NAFLD remains unclear, and as a result, there is currently no specific therapy for this condition. However, recent studies focus on extracellular vesicles (EVs) as a novelty in their role in cellular communication.

Molecular and Immunological Characterization of Troponin C: An Allergen from .

, a crustacean of substantial importance, is a frequent trigger of food allergies. This study examined the molecular and immunological properties of troponin C from (Scy p TnC) as an allergen. The findings indicated that thermal stability of Scy p TnC comprised 150 amino acids and facilitated the induction of CD63/CD203c in basophils from crab allergy patients.

Mode-informed complex-valued neural processes for matched field processing.

A complex-valued neural process method, combined with modal depth functions (MDFs) of the ocean waveguide, is proposed to reconstruct the acoustic field. Neural networks are used to describe complex Gaussian processes, modeling the distribution of the acoustic field at different depths. The network parameters are optimized through a meta-learning strategy, preventing overfitting under small sample conditions (sample size equals the number of array elements) and mitigating the slow reconstruction speed of Gaussian processes (GPs), while denoising and interpolating sparsely distributed acoustic field data, generating dense field data for virtual receiver arrays.

Innovative Application of Electrokinetic Treatment on Montmorillonite Modification: Effects on pH, Current, and Swelling Properties of Ca-Montmorillonite.

This study evaluates the feasibility of converting Ca-montmorillonite into Na-montmorillonite through electroosmosis. Comprehensive analyses of current, pH, ζ-potential, and ethylene glycol expansion were conducted to investigate the macro- and microscale effects of electroosmosis. The results demonstrate that electroosmosis effectively reduces the swelling properties of montmorillonite.

Synthesis and diverse crystal packing in o-, m- and p-bis(carbonylthioureido)benzenes containing bisferrocenes.

Three bisferrocene-based bis(acylthiourea) positional isomers, namely, 1,2-bis(ferrocenylcarbonylthioureido)benzene (1), 1,3-bis(ferrocenylcarbonylthioureido)benzene (2) and 1,4-bis(ferrocenylcarbonylthioureido)benzene (3), all [Fe(CH)(CHNOS)], have been synthesized via facile nucleophilic addition reactions of 2.3 equivalents of ferrocenoyl isothiocyanate with o-, m- and p-phenylenediamine, respectively. The structures of the three new synthesized isomers were fully characterized by H NMR, C NMR, IR and UV-Vis spectroscopy, elemental analyses and cyclic voltammetry.

Variation and assembly mechanisms of skin and cave environmental fungal communities during hibernation periods.

Animal skin acts as the barrier against invasion by pathogens and microbial colonizers. Environmental microbiota plays a significant role in shaping these microbial communities, which, in turn, have profound implications for host health. Previous research has focused on characterizing microorganisms on bats' skin and in their roosting environments, particularly bacterial communities.

Valence State Hydrogen Channel Enhances Sustained and Controllable Electrocatalytic Hydrogen Evolution in Diabetic Skin Wound Healing.

Diabetes significantly increases the risk of serious health issues, including prolonged skin inflammation and delayed wound healing, owing to inferior glucose control and suppression of the immune system. Although traditional hydrogen (H2) therapy is slightly effective, its ability to tailor the release of H2 on the skin is limited. Accordingly, this study proposed a novel strategy for electrocatalytic H2 release under neutral conditions to promote wound healing in diabetic mice and rabbit.

Boron's Role in Diminishing Cadmium Concentrations in Rice ( ): Insights into Absorption Inhibition and Ripening Promotion.

Boron, a crucial element for plant growth, has been demonstrated to mitigate cadmium (Cd) absorption in rice seedlings. However, its impact on Cd accumulation in rice grains and the underlying regulatory mechanisms remain poorly understood. The current study explored the roles of boron in reducing Cd accumulation and promoting ripening in rice through pot and hydroponic experiments.

MA104 cell line is permissive for human bocavirus 1 infection.

Human bocavirus 1 (HBoV1) has appeared as an emerging pathogen, causing mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children and immunocompromised individuals. The lack of cell lines suitable for culturing replicative viruses hinders research on HBoV1. Here, we characterized the susceptibility to HBoV1 of 29 human and 7 animal cell lines, and identified a permissive cell line, MA104.

Global Gac/Rsm regulatory system activates the biosynthesis of mupirocin by controlling the MupR/I quorum sensing system in sp. NCIMB 10586.

The biosynthesis of mupirocin, a clinically significant antibiotic produced by sp. NCIMB 10586, is activated by the -acyl homoserine lactone (AHL) MupR/I quorum sensing (QS) system. However, to date, limited research has focused on the influence of global regulators such as the GacS/A two-component system (TCS) on the MupR/I QS system or mupirocin biosynthesis.

Enhancing selectivity of β-O-4 bond cleavage for lignin depolymerization a sacrificial anode.

Herein, we report a novel electrochemical hydrogenolysis method for β-O-4 bond cleavage by using carbon foam as the cathode and waste aluminum as the anode. The reaction takes place at the cathode, producing ketones and phenolic compounds. Employing waste aluminum as the anode could avoid anodic excessive oxidation of phenols.

Base-Promoted [4 + 1 + 1] Multicomponent Tandem Cycloaddition of -Substituted Nitroarenes, Aldehydes, and Ammonium Salts To Access 2,4-Substituted Quinazoline Frameworks.

We report a base-promoted, metal-free multicomponent tandem reaction, involving a [4 + 1 + 1] cycloaddition process between -substituted nitroarenes, aldehydes, and ammonium salts. Modifying the substituents on the nitroaromatic compounds effectively provides structurally diverse 2-substituted and 4-alkenylquinazolines with good to excellent yields (77%-90% and quinazoline 51 examples) and high tolerance for various inorganic ammonium salts (13 examples, such as NH·HO, NHCl, and NHHF). A new method for constructing 2,4-substituted quinazoline compounds with high selectivity from simple nitrogen source compounds was developed, and the reaction can be scaled up to a gram scale.

Mannuronate Oligosaccharides Ameliorate Experimental Colitis and Secondary Neurological Dysfunction by Manipulating the Gut-Brain Axis.

Microbiota dysfunction induces intestinal disorders and neurological diseases. Mannuronate oligosaccharides (MAOS), a kind of alginate oligosaccharide (AOS), specifically exert efficacy in shaping gut microbiota and relieving cognitive impairment. However, the key regulatory factors involved, such as the specific strains and metabolites as well as their regulatory mechanisms, remain unclear at present.

Clinicopathologic Characteristics of 40 Cases of Juvenile Conjunctival Nevus.

Aims: Limited studies have been conducted on juvenile conjunctival nevus (JCN) in Asian populations. This study aims to investigate the clinical and pathologic characteristics of JCN cases among the Han ethnicity in northwest China, providing insights for pathologists and ophthalmologists in diagnosing this condition.

Methods: A subset of conjunctival nevi in children and adolescents, characterized by a confluent growth pattern and lack of maturation, was identified and defined as JCN.

Harnessing Anti-Inflammatory and Regenerative Potential: GelMA Hydrogel Loaded with IL-10 and Kartogenin for Intervertebral Disc Degeneration Therapy.

Intervertebral disc degeneration (IVDD) is a major contributor to chronic back pain and disability, with limited effective therapeutic options. Current treatment options, including conservative management and surgical interventions, often fail to effectively halt disease progression and come with notable side effects. IVDD is characterized by the breakdown of the extracellular matrix (ECM) and the infiltration of inflammatory cells, which exacerbate disc degeneration.

Enhancing Waterproof Food Packaging with Janus Structure: Lotus Leaf Biomimicry and Polyphenol Particle Technology for Vegetable Preservation.

With the increasing demand for improved food preservation, conventional waterproof food packaging has proven inadequate because of its limited functionality. Although incorporating features such as antibacterial and antioxidant properties into packaging enhances protection, it can compromise the hydrophobicity of the involved material, thereby increasing the risk of contamination from external sources. To address this challenge, a robust and reliable barrier capable of simultaneously integrating multiple protective functions is required.

Erbium: key to simultaneously achieving superior temperature-stability and high magnetic properties in 2 : 17-type permanent magnets.

To address the demands of rapidly advancing precision instruments requiring higher efficiency and miniaturization, permanent magnets must exhibit exceptional energy density, temperature stability, high magnetic energy product [()], and adequate coercivity (). Herein, we design rare earth Er-based magnets (2 : 17-type Er-magnets) with a composition of (Er, Sm)(Co, Fe, Cu, Zr). Erbium-based compounds (ErCo) offer a unique combination of temperature compensation and high saturation magnetization compared to other heavy rare earth elements, resulting in 2 : 17-type Er-magnets with superior temperature stability in and ().

Therapy Potential of Oblique Lumbar Interbody Fusion 360 for Severe Lumbar Spinal Stenosis.

Objectives: The advent of O-arm navigation optimized the oblique lumbar interbody fusion (OLIF) procedure, allowing the operator to simultaneously perform OLIF and percutaneous posterior pedicle screw implantation without patient position change, thus improving the fluency and accuracy of the OLIF procedure (called as OLIF360). Nevertheless, a consensus regarding its suitability for patients with severe spinal stenosis remains elusive. This study aims to investigate the clinical efficacy of OLIF360 and its imaging changes in severe lumbar spinal stenosis cases.

Identification of Proteoforms Related to Flower Petaloid Through Proteogenomic Strategy.

is an aquatic plant with a high ornamental value due to its flower. Despite the release of several versions of the lotus genome, its annotation remains inefficient, which makes it difficult to obtain a more comprehensive knowledge when -omic studies are applied to understand the different biological processes. Focusing on the petaloid of the lotus flower, we conducted a comparative proteomic analysis among five major floral organs.

The Training and Evaluation of the "Dual-Index" Screening Method for Neonatal Congenital Heart Disease: A Multi-Center Study in China.

Background: This study aimed to enhance the scope of neonatal congenital heart disease (CHD) screening by evaluating the effectiveness of training personnel in CHD screening using the "dual-index" method, combining pulse oximetry with cardiac murmur auscultation.

Methods: From 2019 to 2022, a total of 2374 screening personnel from the Xinjiang, Yunnan, Hainan, Fujian, and Anhui provinces underwent training in neonatal CHD screening using the "dual-index" method, which involves pulse oximetry and cardiac murmur auscultation. Pre- and post-training assessments were conducted using a neonatal CHD screening knowledge questionnaire, distributed through the Questionnaire Star platform, to evaluate the impact of the training.

Residual Dynamics of Chlorantraniliprole and Fludioxonil in Soil and Their Effects on the Microbiome.

The increased use of chlorantraniliprole and fludioxonil has sparked concerns about their residues and impact on the soil microbiome, highlighting an urgent issue requiring attention. This study investigates the residue dynamics of corn after chlorantraniliprole and fludioxonil treatments, as well as their effects on soil enzyme activity and microbial community structure. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis showed a significant decrease in chlorantraniliprole and fludioxonil residues in the soil after combined application, especially with chlorantraniliprole.

Single and Synergistic Effects of Microplastics and Difenoconazole on Oxidative Stress, Transcriptome, and Microbiome Traits in Honey Bees.

Microplastics (MPs) and pesticides are identified as two environmental pollutants. In the present study, we showed evidence of toxic effects on honey bees from chronic oral exposure to food containing difenoconazole alone (Dif) and in a binary mixture with polystyrene (PS)-MPs (Dif + PS). We observed a disrupted gut microbial community structure in bees after difenoconazole exposure, and the gut microbiota structure richness increased at the phylum and genus levels in Dif + PS group.

Friction Reduction and Antiwear Mechanisms of Cerium Sulfide Nanosheets under Different Sliding Conditions.

With the rapid development of modern industry, traditional lubricants often require a variety of additives to be used in conjunction with each other, which not only increases the cost but also causes a waste of resources. Therefore, the development of a lubricant additive with both a dyeing function and an antiwear and friction reduction performance can more effectively meet the industrial needs. Cerium sulfide (CeS), with its excellent photostability, weather resistance, thermal stability, and nontoxicity, shows great potential as an environmentally friendly pigment.