Riverine antibiotic resistome along an anthropogenic gradient.

The introduction of antibiotic-resistant bacteria into riverine systems through the discharge of wastewater treatment plant (WWTP) effluent and agricultural waste poses significant health risks. Even when not pathogenic, these bacteria can act as reservoirs for antibiotic resistance genes (ARGs), transferring them to pathogens that infect humans and animals. In this study, we used fluorescence hybridization, qPCR, and metagenomics to investigate how anthropogenic activities affect microbial abundance and the resistome along the Holtemme River, a small river in Germany, from near-pristine to human-impacted sites.

Genomic identification of a pair of multidrug-resistant but non-pathogenic serovar Goldcoast isolates in southeast China.

Introduction: is an important foodborne pathogen that can induce severe diseases such as gastrointestinal disease and typhoid fever. Accumulating evidence revealed that 's resistance to antibiotics also seriously affects human health. Pathogenic serovar Goldcoast ( Goldcoast) was first detected in 2010 in China and was predicted to have an increasing tendency.

Dynamics of endophytic fungi composition in seeds during storage and growth, and responses of seedlings to phytohormones.

Introduction: Endophytic fungi exhibit diverse interactions with plants, from pathogenic to mutualistic symbiosis, and the community composition is regulated by phytohormones. Yet, the composition and dynamics of endophytic fungi in (PPC) during fresh seed (FD), sand-stored seed (SSD), and seedling (SS) stages remain unclear. Similarly, the overall impact of phytohormones on the management of endophytic fungal communities is yet to be elucidated.

Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ.

The study of bacteriophage (phage) gene products and their effects on the host helps to better understand the phage-host relationship and provides clues for the development of new antimicrobial proteins. In this study, we focused on a small protein named Icd with 73 amino acids from phage P1. It inhibits the growth of and rapidly blocks the formation of Z-ring.

sp. nov., a new species associating with sugar beet root rot in Heilongjiang Province, China.

Introduction: Sugar beet ( L.) is an economically important crop grown worldwide, but its production is threatened by root rot diseases caused by soil-borne fungi. This study aimed to identify and characterize a new pathogen causing root rot in sugar beet in Heilongjiang Province, China.

Dynamic changes in the gut microbiota of SPF Bama piglets during breast and formula feeding.

The gut microbiota plays a crucial role in the growth performance, health status, and welfare of pigs. Breast milk is a key factor in the colonization of gut microbiota and the overall health of newborn piglets. With advancements in breeding technology, formula milk has been widely adopted as a substitute for breast milk.

Biliary-intestinal anastomosis leads to alterations in intestinal flora and its flora metabolites and increases the risk of long-term postoperative complications: a case-control study.

Objective: Pancreaticoduodenectomy (PD) is a major surgical intervention that encompasses the resection of multiple organs and the reconstruction of the digestive tract, with reconstructive procedures including pancreatico-enteric, bilioenteric, and gastroenteric anastomoses. Prior research has documented a high incidence of long-term complications following PD, which significantly impact patient prognosis and survival, however, the underlying mechanisms remain elusive. Evidence from previous studies suggests that biliary-intestinal anastomosis modifies biliary tract anatomy, altering bile flow into the gut and potentially affecting the gut microbiota and its metabolites.

High prevalence of carbapenem-resistant and identification of a novel VIM-type metallo-β-lactamase, VIM-92, in clinical isolates from northern China.

Carbapenem-resistant (CRPA) has become a serious global health concern due to the limited treatment options. The primary resistance mechanism in CRPA involves the production of metallo-β-lactamases (MBLs), making MBL-producing a significant component of CRPA cases. To understand the prevalence of CRPA in hospitals in northern China, we conducted a preliminary screening and identification of CRPA in 143 clinical isolates of collected from various departments of a tertiary hospital between 2021 and 2023, analyzing CRPA resistance trends in certain regions of northern China during this period.

Effect of the CSFV NS5A protein on key proteins in the MAPK and PI3K-mTOR signaling pathways in porcine macrophages.

Classical swine fever (CSF) is a highly contagious disease caused by classical swine fever virus (CSFV). NS5A, a non-structural protein of CSFV, plays an important role in regulating viral replication and protein translation. The purpose of this study was to investigate the effects of the CSFV NS5A protein on key proteins in the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)-mechanistic target of rapamycin (mTOR) pathways in porcine macrophages.

Optical attenuation coefficient decorrelation-based optical coherence tomography angiography for microvascular evaluation of Alzheimer's disease on mice.

Significance: The deep cortical microvasculature is closely linked to the pathogenesis of Alzheimer's disease (AD). However, tail artifacts from superficial cortical vessels often interfere with detecting deep vessels in optical coherence tomography angiography (OCTA) imaging. A more accurate method to assess deep cortical vasculature is crucial for understanding its relationship with AD onset.

Explore autophagy-related lncRNA-miRNA-mRNA ceRNA networks for diagnosis of early-onset schizophrenia through transcriptome analysis.

Background: The severe functional impairment and poor prognosis of early-onset schizophrenia (EOS) create a great need to identify effective biomarkers for early diagnosis in young psychiatric patients. Current research indicates a potential link between loss of autophagy function and emotional and behavioral abnormalities in individuals with psychiatric disorders.

Materials And Methods: This study aimed to explore diagnostic autophagy-related endogenous competitive RNA (ceRNA) networks for EOS patients.

Psychotic symptoms in Chinese patients with somatic symptom disorder: prevalence, risk factors, and associated conditions.

Psychotic symptoms are prevalent in individuals with various mental health disorders and frequently lead to adverse outcomes. In this study, we assessed the prevalence of psychotic symptoms and its associated conditions in a large sample of Chinese patients with somatic symptom disorder (SSD), which has not been examined systemically. We recruited 899 patients with SSD.

Efficacy and safety of electroacupuncture on treating mild to moderate first-episode depression: a study protocol for a randomized controlled trial.

Introduction: Only 30%-40% of patients with first-episode depression recover after taking antidepressants. Acupuncture is a clinically recognized treatment for depression, but its effect on first-episode depression remains unknown. This randomized controlled trial is designed to investigate the efficacy and safety of electroacupuncture (EA) compared with escitalopram (ESC) in treating patients with mild to moderate first-episode depression.

The relationship between interpersonal security and social media dependence: a moderated mediation model.

Background: Interpersonal security is an important psychological factor influencing social media use. However, little is known about the mediating and moderating mechanisms linking Interpersonal security and social media dependence.

Objective: The present study explored the mediating role of negative rumination between interpersonal safety and social media dependence, as well as cohort differences in sibling conditions as moderators.

Further validation of the Chinese short Warwick Edinburgh mental wellbeing scale in the adult population of Macau: an application of classic test theory and item response theory.

Background: This study aims to validate the Chinese version of the Short Warwick-Edinburgh Mental Well-being Scale (SWEMWBS) by employing both Classical Test Theory (CTT) and Item Response Theory (IRT) approaches.

Methods: Data were gathered through a population-based, cross-sectional health survey using an online self-reported questionnaire. The scale underwent Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA).

Stigma and self-perceived burden in postoperative liver cancer patients: the mediating role of financial toxicity.

Purpose: To investigate the mediating effect of financial toxicity on stigma and self-perceived burden in patients with liver cancer after surgery.

Methods: Using a convenience sampling method, 236 postoperative liver cancer patients treated at a tertiary hospital in Nanjing from April 2024 to July 2024 were selected for the study. Questionnaires were administered, including a general information survey, the Social Impact Scale (SIS), the Self-Perceived Burden Scale for Cancer Patients (SPBS-CP), and the Comprehensive Score for Financial Toxicity-Functional Assessment of Chronic Illness Therapy Version 2 (COST-FACIT-V2).

Risk factors for steroid-induced osteonecrosis of the femoral head in children with immune kidney disease.

Objective: To investigate the risk factors for steroid-induced osteonecrosis of the femoral head (SONFH) after glucocorticoid (GC) therapy in children with immune kidney diseases.

Methods: This retrospective study included patients (1-18 years) diagnosed with immune kidney disease treated with GCs from January 2012 to July 2022 in our hospital. Data, such as sex, age and body weight at the first GC treatment, and the GC dose, were collected.

Preoperative localization of parathyroid glands in secondary hyperparathyroidism: correlations between 99mTc-MIBI-SPECT/CT, ultrasound, and pathological characteristics.

Objective: To investigate the association between imaging findings and histopathological characteristics of parathyroid glands in patients with secondary hyperparathyroidism (SHPT).

Methods: Seventy-four glands from 21 patients with SHPT who underwent parathyroidectomy were evaluated for their pathological characteristics. The detection rates of parathyroid glands using ultrasound (US) and 99Tc-MIBI-SPECT/CT (MIBI) were compared.

Association Between Progesterone Level on Trigger Day to Basal Progesterone Ratio and in vitro Fertilization-Embryo Transfer Outcomes in Antagonist Protocols.

Objective: To investigate the relationship between the progesterone level on trigger day (P) to basal progesterone (bP) ratio and the outcomes of in vitro fertilization-embryo transfer (IVF-ET).

Methods: A retrospective analysis was conducted on women who received an antagonist protocol, with a P less than 1.5 ng/mL and who underwent fresh embryo transfer.

Differences in Pelvic Organ Prolapse and Diastasis Recti Abdominis Between Primiparous and Age-Matched second-Parous Females After Vaginal Delivery: A Retrospective Cohort Study.

Purpose: This study aims to investigate the differences in pelvic floor dysfunction (PFD) and diastasis recti abdominis (DRA) between primiparous and second-parous females with known risk factors controlled.

Patients And Methods: 49 primiparous and 49 age-matched second-parous postpartum after spontaneous vaginal delivery were included between October and December 2023. Independent variables include weight, height, body mass index (BMI), fetal weight, gestational weight gain (GWG).

A spatial and temporal transformer-based EEG emotion recognition in VR environment.

With the rapid development of deep learning, Electroencephalograph(EEG) emotion recognition has played a significant role in affective brain-computer interfaces. Many advanced emotion recognition models have achieved excellent results. However, current research is mostly conducted in laboratory settings for emotion induction, which lacks sufficient ecological validity and differs significantly from real-world scenarios.

A longitudinal study on the effect of aerobic exercise intervention on the inhibitory control in college students with internet addiction.

Objectives: This study aimed to investigate the effects of aerobic exercise on reactive inhibitory control in college students with internet addiction, examining both behavioral and electrophysiological changes over time.

Methods: A longitudinal study design was adopted, involving 48 male college students with internet addiction who were randomly assigned to either a control group or an experimental group. Participants in the experimental group engaged in 40 min aerobic cycling sessions three times per week for 12 weeks, while the control group maintained their usual physical activity levels without any intervention.

Comparative genomics of three non-hematophagous leeches ( spp.) with emphasis on antithrombotic biomolecules.

Leeches are well known for blood-feeding habits and are widely used for medicinal purposes as they secrete various antithrombotic substances. However, some leeches exhibit non-hematophagous habits and their significance for medicinal use is controversial. Here we provide the chromosome-level genomes of two non-hematophagous leeches, and , and, in combination with previous results from , we compared these genomes with an emphasis on antithrombotic biomolecules.

Morphological description and DNA barcoding research of nine species.

Introduction: plants are highly valued for their ornamental qualities. However, traditional morphological identification methods are inefficient for discriminating species. DNA barcoding has emerged as a powerful alternative for species identification, but research on DNA barcodes is still limited.

Major adverse cardiovascular events or venous thromboembolism in patients with rheumatoid arthritis initiating biological or targeted synthetic disease-modifying antirheumatic drugs: a nationwide, population-based cohort study.

Background: Rheumatoid arthritis (RA) is complicated by a high risk of cardiovascular disease and requires the initiation of biological or targeted synthetic disease-modifying antirheumatic drugs (b/tsDMARDs) for persistently active disease despite first-line therapies. The influence of b/tsDMARDs, especially tsDMARDs, on cardiovascular risk in Taiwanese patients with RA remains unclear.

Objectives: To compare the risk of major cardiovascular adverse events (MACEs) or venous thromboembolism (VTE) amongst RA patients initiating approved b/tsDMARDs for up to 5 years.