GenAI synthesis of histopathological images from Raman imaging for intraoperative tongue squamous cell carcinoma assessment.

The presence of a positive deep surgical margin in tongue squamous cell carcinoma (TSCC) significantly elevates the risk of local recurrence. Therefore, a prompt and precise intraoperative assessment of margin status is imperative to ensure thorough tumor resection. In this study, we integrate Raman imaging technology with an artificial intelligence (AI) generative model, proposing an innovative approach for intraoperative margin status diagnosis.

Flood change detection model based on an improved U-net network and multi-head attention mechanism.

This work aims to improve the accuracy and efficiency of flood disaster monitoring, including monitoring before, during, and after the flood, to achieve accurate extraction of flood disaster change information. A modified U-Net network model, incorporating the Transformer multi-head attention mechanism (TM), is developed specifically for the characteristics of Synthetic Aperture Radar (SAR) images. By integrating the TM, the model effectively prioritizes image regions relevant to flood disasters.

Standard operating procedure combined with comprehensive quality control system for multiple LC-MS platforms urinary proteomics.

Urinary proteomics is emerging as a potent tool for detecting sensitive and non-invasive biomarkers. At present, the comparability of urinary proteomics data across diverse liquid chromatography-mass spectrometry (LC-MS) platforms remains an area that requires investigation. In this study, we conduct a comprehensive evaluation of urinary proteome across multiple LC-MS platforms.

Cardiovascular disease attenuates the protective effect of folate on global cognitive function in an elderly population: a cross-sectional study.

Research suggests that folic acid contributes to improving cognitive function. However, there is a lack of systematic research on the association of dietary intake of folate and serum, and red blood cell (RBC) folate levels with global cognitive impairment (CoI) in the elderly population. Importantly, excessive supplementation with folate among American adults at high risk of cardiovascular disease (CVD) may have harmful effects.

Recovery of nearly 3,000 archaeal genomes from 152 terrestrial geothermal spring metagenomes.

Terrestrial geothermal springs, reminiscent of early Earth conditions, host diverse and abundant populations of Archaea. In this study, we reconstructed 2,949 metagenome-assembled genomes (MAGs) from 152 metagenomes collected over six years from 48 geothermal springs in Tengchong, China. Among these MAGs, 1,431 (49%) were classified as high-quality, while 1,518 (51%) were considered as medium-quality.

An improved sparrow search algorithm with multi-strategy integration.

Addressing the shortcomings of the Sparrow Search Algorithm (SSA), such as low accuracy of convergence and tendency of falling into local optimum, a Multi-strategy Integrated Sparrow Search Algorithm (MISSA) is proposed. In this method, by improving the black-winged kite algorithm and applying it to the producer's position update formula, an improved search strategy (ISS) is firstly proposed to enhance search ability. Secondly, a new strategy inspired by the Coot algorithm, called the group follow strategy (GFS), is proposed to improve the ability to jump out of the local optimum.

Overexpression of miR-124 enhances the therapeutic benefit of TMZ treatment in the orthotopic GBM mice model by inhibition of DNA damage repair.

Glioblastoma (GBM) is the most common malignant primary brain cancer with poor prognosis due to the resistant to current treatments, including the first-line drug temozolomide (TMZ). Accordingly, it is urgent to clarify the mechanism of chemotherapeutic resistance to improve the survival rate of patients. In the present study, by integrating comprehensive non-coding RNA-seq data from multiple cohorts of GBM patients, we identified that a series of miRNAs are frequently downregulated in GBM patients compared with the control samples.

Chromosome-level genome assembly and annotation of the gynogenetic large-scale loach (Paramisgurnus dabryanus).

The large-scale loach (Paramisgurnus dabryanus; Cypriniformes: Cobitidae) is primarily distributed in East Asia. It is an important economic fish species characterized by fast growth, temperature-dependent sex determination and the ability to breathe air. Currently, molecular mechanism studies related to some aspects such as sex determination, toxicology, feed nutrition, growth and genetic evolution have been conducted.

Enhancer-driven Shh signaling promotes glia-to-mesenchyme transition during bone repair.

Plp1-lineage Schwann cells (SCs) of peripheral nerve play a critical role in vascular remodeling and osteogenic differentiation during the early stage of bone healing, and the abnormal plasticity of SCs would jeopardize the bone regeneration. However, how Plp1-lineage cells respond to injury and initiate the vascularized osteogenesis remains incompletely understood. Here, by employing single-cell transcriptional profiling combined with lineage-specific tracing models, we uncover that Plp1-lineage cells undergoing injury-induced glia-to-MSCs transition contributed to osteogenesis and revascularization in the initial stage of bone injury.

The cryptic lncRNA-encoded microprotein TPM3P9 drives oncogenic RNA splicing and tumorigenesis.

Emerging evidence demonstrates that cryptic translation from RNAs previously annotated as noncoding might generate microproteins with oncogenic functions. However, the importance and underlying mechanisms of these microproteins in alternative splicing-driven tumor progression have rarely been studied. Here, we show that the novel protein TPM3P9, encoded by the lncRNA tropomyosin 3 pseudogene 9, exhibits oncogenic activity in clear cell renal cell carcinoma (ccRCC) by enhancing oncogenic RNA splicing.

Impacts of irrigation expansion on moist-heat stress based on IRRMIP results.

Irrigation rapidly expanded during the 20 century, affecting climate via water, energy, and biogeochemical changes. Previous assessments of these effects predominantly relied on a single Earth System Model, and therefore suffered from structural model uncertainties. Here we quantify the impacts of historical irrigation expansion on climate by analysing simulation results from six Earth system models participating in the Irrigation Model Intercomparison Project (IRRMIP).

Temperature seasonality regulates organic carbon burial in lake.

Organic carbon burial (OCB) in lakes, a critical component of the global carbon cycle, surpasses that in oceans, yet its response to global warming and associated feedbacks remains poorly understood. Using a well-dated biomarker sequence from the southern Tibetan Plateau and a comprehensive analysis of Holocene total organic carbon variations in lakes across the region, here we demonstrate that lake OCB significantly declined throughout the Holocene, closely linked to changes in temperature seasonality. Process-based land surface model simulations clarified the key impact of temperature seasonality on OCB in lakes: increased seasonality in the early Holocene saw warmer summers enhancing ecosystem productivity and organic matter deposition, while cooler winters improved organic matter preservation.

Development and validation of a prognostic nomogram for predicting of patients with acute sedative-hypnotic overdose admitted to the intensive care unit.

To develop and evaluate a predictive model for intensive care unit (ICU) admission among patients with acute sedative-hypnotic overdose. We conducted a retrospective analysis of patients admitted to the emergency department of West China Hospital, Sichuan University, between October 11, 2009, and December 31, 2023. Patients were divided into ICU and non-ICU groups based on admission criteria including the need for blood purification therapy, organ support therapy (ventilatory support, vasoactive drugs, renal replacement therapy, artificial liver), or post-cardiopulmonary resuscitation.

A principle-based framework to determine countries' fair warming contributions to the Paris Agreement.

Equity is a cornerstone of global climate policy, yet differing perspectives mean that international agreement on how to allocate mitigation efforts remains elusive. A rich literature informs this question, but a gap remains in approaches that appropriately consider non-CO emissions and their warming contributions. In this study, we address this gap and define a global warming budget applicable to all anthropogenic greenhouse gases that is allocated to countries based on principles drawn from international treaties and environmental law.

Isovitexin targets SIRT3 to prevent steroid-induced osteonecrosis of the femoral head by modulating mitophagy-mediated ferroptosis.

The death of osteoblasts induced by glucocorticoid (GC)-mediated oxidative stress plays a crucial role in the development of steroid-induced osteonecrosis of the femoral head (SIONFH). Improving bone formation driven by osteoblasts has shown promising outcomes in the prognosis of SIONFH. Isovitexin has demonstrated antioxidant properties, but its therapeutic effects on GC-induced oxidative stress and SIONFH remain unexplored.

Exploring the effects of aromatherapy and acupoint herbal patching on fatigue and sleep disorders in people living with HIV: a pilot randomized trial.

The purpose of this study was to investigate the effects of aromatherapy and acupoint herbal patching on fatigue and sleep disorders in people living with HIV (PLWH). Aromatherapy and acupoint herbal patching are ancient alternative therapies in traditional Chinese medicine. We randomly selected 90 patients from the Nanjing Public Health Medical Center in China and divided them into three groups: aromatherapy group, acupoint herbal patching group and control group.

[Application of ambulatory surgery management mode in total laparoscopic ileostomy reversal surgery].

To explore the safety of application of ambulatory surgery management mode in total laparoscopic ileostomy reversal surgery. A retrospective analysis of clinical data was conducted on patients who had undergone total laparoscopic ileostomy reversal surgery at the First Affiliated Hospital of China Medical University from May 1, 2023, to March 31, 2024. A total of 34 patients were included in the study, comprising 22 males and 12 females, with the age of (60.

[Clinical characteristics and prognosis of type 1 infratentorial superficial siderosis].

A total of 7 patients with type 1 infratentorial superficial siderosis(iSS-1)in Memory Clinic at Huashan Hospital, Fudan University from March 2019 to March 2023 were respectively collected to analyze the clinical characteristics and treatment, and followed up for 12 months. There were 7 patients, 2 males and 5 females, aged 56 (49-60) years. The 4 patients who underwent cognitive assessment had varying degree of cognitive impairment, with 2 patients exhibiting severe executive function impairment.

[Effects of long non-coding RNA KLHL7-AS1 on the proliferation and apoptosis of nucleus pulposus cells under the environment of oxidative stress and its mechanism].

To investigate the effects of long non-coding RNA KLHL7-AS1 (LncRNA KLHL7-AS1) on the proliferation and apoptosis of nucleus pulposus cells under oxidative stress and its mechanisms. Human nucleus pulposus cells (HUM-iCell-s012) were divided into 4 groups, and unoxidized nucleus pulposus cells were transfected with an empty pcDNA vector (pcDNA-control) to serve as the blank control group. Based on previous studies on oxidative stress-induced nucleus pulposus cell senescence and preliminary experiments, oxidative stress was induced by treating nucleus pulposus cells with 400 μmol/L HO.

[Analysis of outcomes in adult deceased donor dual kidney transplantation].

To investigate the efficacy of dual kidney transplantation (DKT) from adult donors. Clinical data of adult DKT donors and recipients in the Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology from March 2015 to June 2024 were retrospectively analyzed. The patients were followed up until September 2024.

[The clinical significance of resolvin D1 in patients with systemic lupus erythematosus treated with Belimumab].

To explore the changes and significance of resolvin D1 (RvD1) in the treatment of systemic lupus erythematosus (SLE) with Belimumab. The clinical data from patients with moderate to severe disease activity SLE who received oral stable doses of glucocorticoids (≤10 mg/d) and/or immunosuppressants for more than 3 months at the outpatient or inpatient department of the First Affiliated Hospital of Soochow University from January 2022 to November, 2023 were retrospectively collected. All patients were treated with 10 mg/kg intravenous infusion of Belimumab.

[Expert consensus on the diagnosis and treatment of neurobrucellosis (2025 edition)].

Neurobrucellosis is a neurological disorder caused by Brucella infection. It typically occurs as part of the multisystem involvement of brucellosis, or may also present as brucellosis. The existing clinical practice guidelines and expert consensus on human brucellosis are outdated and provide limited guidance specific to the diagnosis and management of neurobrucellosis, failing to meet the evolving needs of healthcare providers and patients.

[Value of comprehensive evaluation of bladder structure and function in children by multi-modal ultrasound].

To comprehensively evaluate the structure and function of the bladder wall in children using multi-modal ultrasound (MMU). A total of 110 children without urinary symptoms admitted to the First Affiliated Hospital of Zhengzhou University from January 2022 to June 2023 were included prospectively. MMU was performed and the vesical volume (VV), bladder wall thickness (BWT), shear wave velocity (SWV) of the anterior wall, resistance index (RI) and vascularization index (VI) were measured in the filled and emptied bladder respectively, and the ultrasound bladder compliance (UBC) was also calculated.

[Efficacy of inverted door flap combined with transcanal approach to the tympanic antrum technique for the treatment of middle ear cholesteatoma under endoscope].

This study aimed to investigate the efficacy of inverted door flap (IDF) combined with transcanal approach to the tympanic antrum (TCAA) technique under the endoscope for treatment of middle ear cholesteatoma. Outcomes of patients treated with combined techniques at the First People's Hospital of Foshan City between March 2021 and March 2023 were evaluated. A total of 31 patients (33 ears, 16 males and 15 females) aged (42.

[Effect of SMARCA4 mutations on the outcomes of patients with advanced EGFR mutant lung adenocarcinoma].

To investigate the impact of SMARCA4 mutations on the outcomes of patients with advanced lung adenocarcinoma with epidermal growth factor receptor (EGFR) mutations. In the Memorial Sloan Kettering Cancer Center (MSK) MetTropism study, 960 patients with advanced EGFR-mutated lung adenocarcinoma were screened and included in the MSK cohort, composing of 313 males and 647 females, with a median [(, )] age of 64 (56, 72) years. A retrospective analysis was conducted on the data of 178 patients with advanced EGFR-mutated lung adenocarcinoma who received EGFR tyrosine kinase inhibitors (TKIs) treatment in the Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, from January 2018 to December 2022.