One-step transformation of CO to methane by Escherichia coli with a synthetic biomethanation module.

The biomethanation process is widely recognized as a significant approach to mitigating carbon dioxide emissions while simultaneously generating methane. However, only a few microorganisms that required intricate culturing conditions were identified for biomethanation. Here, Escherichia coli that featured easy cultivation and versatile chassis was genetically modified for biomethanation for the first time.

Knowledge domain and frontier trends of artificial intelligence applied in solid organ transplantation: A visualization analysis.

Background: Solid organ transplantation (SOT) is vital for end-stage organ failure but faces challenges like organ shortage and rejection. Artificial intelligence (AI) offers potential to improve outcomes through better matching, success prediction, and automation. However, the evolution of AI in SOT research remains underexplored.

A systematic review of measurement tools and senior engagement in urban nature: Health benefits and behavioral patterns analysis.

The engagement of senior citizens with urban nature has been shown to provide multiple health benefits and mitigate health issues associated with demographic aging. This review utilized the PRISMA methodology to systematically analyze the relationship between monitoring tools, seniors' behaviors in urban nature, and influencing factors. The main findings are as follows: (1) 4 main types, including self-reports, on-site observations, sensors, and third-party data, and 24 sub-types of measurement tools: ranging from questionnaires to crowdsourced imagery services.

Longitudinal relationship between internet addiction and psychotic-like experiences among Chinese college students.

Background: While previous cross-sectional studies have suggested a link between psychotic-like experiences (PLEs) and internet addiction (IA), longitudinal evidence remains scarce. This study aimed to explore the prospective relationship between IA and PLEs among college students.

Method: A total of 636 college students (80 % female) were assessed in November 2022 and again one year later.

Effectiveness of information technology-based cognitive behavioral therapy on depression and anxiety symptoms among older adults: Systematic review and meta-analysis.

Background: Depression and anxiety are prevalent among older adults. However, most older adults have poor access to age-specific mental health services. While Information technology-based Cognitive Behavioral Therapy (ICBT) has shown promise as an accessible alternative to face-to-face interventions, its effectiveness specifically within the older adults warrants further investigation.

Mesencephalic astrocyte-derived neurotrophic factor inhibits neuroinflammation through autophagy-mediated α-synuclein degradation.

Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder marked by the progressive loss of dopamine neurons in the substantia nigra. α-synuclein (SNCA) aggregation-induced microglia activation and neuroinflammation play vital role in the pathology of PD. Our previous studies showed that mesencephalic astrocyte-derived neurotrophic factor (MANF) could inhibit SNCA accumulation and Lipopolysaccharides (LPS)-induced neuroinflammation, but the specific molecular mechanism remains unclear.

Acidobacteria as the dominant microorganism on the nitrogen-removal wastewater treatment with a low chemical oxygen demand/ammonium nitrogen ratio in biological contact oxidation reactor.

Nitrogen-removal promotion is a significant problem when biological nitrogen removal is used to treat ammonium nitrogen (NH-N) wastewater with a low chemical oxygen demand (COD)/NH-N (C/N) ratio. In this work, the biological nitrogen removal capacity of the biological contact oxidation reactor (BCOR) system was enhanced through the enrichment of Acidobacteria. The system was successfully started from Day 1 to Day 50 and stably operated through temperature, pH, and dissolved oxygen (DO) regulation from Day 51 to Day 254.

Comparative study of Idesia polycarpa Maxim cake meal polysaccharides: Conventional versus innovative extraction methods and their impact on structural features, emulsifying, antiglycation, and hypoglycemic properties.

Idesia polycarpa Maxim (IPM) cake meal, a major by-product of oil extraction, is often discarded in large quantities, resulting in considerable waste. This study explored the extraction of IPM polysaccharides (IPMPs) from cake meal using the innovative ultrasonic-assisted three-phase partitioning (UTPP) method, in comparison with conventional techniques, including acid, medium-temperature alkali, chelating agent, and enzyme extraction methods. The IPMP-UT prepared via UTPP method achieved superior extraction efficiency (10.

Effect of Anterior Chamber Air Injection on Intraocular Lens Stability after Phacovitrectomy with Gas/Air Tamponade.

Purpose: Anterior chamber air injection (ACAI) is a surgical technique used to decrease the occurrence of postoperative intraocular lens (IOL) haptic dislocation following phacovitrectomy with gas/air tamponade. The impact of this technique on IOL stability remains uncertain, prompting the design of this study to investigate further.

Methods: This study included 51 eyes of 51 patients who underwent phacovitrectomy with gas/air tamponade.

Inflammation-induced Generation of Splenic Erythroblast-like Ter- Cells Inhibits the Progression of Acute Lung Injury via Artemin.

Identifying inflammation-induced leukocyte subsets and their derived circulating factors has been instrumental in understanding the progession of ALI/ARDS. Nevertheless, how primary inflammation-induced non-leukocyte populations in distal organs contribute to ALI/ARDS remains poorly defined. Here, we report one population of erythroblast-like cells (Ter-cells) deriving from megakaryocyte- erythroid progenitor cells with a unique Ter-119+CD45-CD71+ phenotype in ALI/ARDS.

Association Between Intergenerational Support, Technology Perception and Trust, and Intention to Seek Medical Care on the Internet Among Chinese Older Adults: Cross-Sectional Questionnaire Study.

Background: Avoiding technological innovation does not simplify life. In fact, using internet-based medical services can enhance the quality of life for older adults. In the context of an aging population and the growing integration of information technology, the demand for internet-based medical services among older adults is gaining increased attention.

Photoredox/Nickel Dual-Catalytic Asymmetric Silylarylation of Alkenes.

The efficient construction of chiral aryl-containing organosilicon frameworks via catalytic enantioselective three-component silylarylation of alkenes remains a great challenge. Herein, a photoredox/nickel dual-catalytic asymmetric protocol has been disclosed by using a chiral biimidazoline (BiIM) as the ligand, silylboranes as the silyl radical precursors, aryl bromides as the coupling partners, and morpholine as the promoter. Remarkably, the reaction features mild and green conditions, high reaction efficiency, and excellent enantioselectivity, enabling the facile synthesis of valuable chiral tropic acid and sila-isoflavanone structures.

Nonlinear effects of resource allocation delay on epidemic spreading in complex networks.

The impact of resource allocation on the dynamics of epidemic spreading is an important topic. In real-life scenarios, individuals usually prioritize their own safety, and this self-protection consciousness will lead to delays in resource allocation. However, there is a lack of systematic research on the impact of resource allocation delay on epidemic spreading.

Autonomous International Classification of Diseases Coding Using Pretrained Language Models and Advanced Prompt Learning Techniques: Evaluation of an Automated Analysis System Using Medical Text.

Background: Machine learning models can reduce the burden on doctors by converting medical records into International Classification of Diseases (ICD) codes in real time, thereby enhancing the efficiency of diagnosis and treatment. However, it faces challenges such as small datasets, diverse writing styles, unstructured records, and the need for semimanual preprocessing. Existing approaches, such as naive Bayes, Word2Vec, and convolutional neural networks, have limitations in handling missing values and understanding the context of medical texts, leading to a high error rate.

Sugarcane Pan-Transcriptome Identifying a Master Gene Regulating Lignin and Sugar Traits.

Sugarcane has the most complex polyploid genome in the world, and sugar-related traits are one of the most important aims in sugarcane breeding. It is essential to construct a representative pan-transcriptome that contains all transcripts of a species for studies on genetic diversity, population expression, and omics analyses in sugarcane. In this study, we constructed the first comprehensive pan-transcriptome for sugarcane, and 8434 highly reliable open reading frames were found, which were not aligned with any published sugarcane genome.

PPI-CoAttNet: A Web Server for Protein-Protein Interaction Tasks Using a Coattention Model.

Predicting protein-protein interactions (PPIs) is crucial for advancing drug discovery. Despite the proposal of numerous advanced computational methods, these approaches often suffer from poor usability for biologists and lack generalization. In this study, we designed a deep learning model based on a coattention mechanism that was capable of both PPI and site prediction and used this model as the foundation for PPI-CoAttNet, a user-friendly, multifunctional web server for PPI prediction.

Primary intracranial extraosseous Ewing's sarcoma with intraspinal metastasis in children: illustrative case.

Background: The diagnosis of intracranial extraosseous Ewing's sarcoma (EES) poses challenges due to the absence of specific clinical and imaging features prior to surgery. It is crucial to differentiate the tumor from other small round cell malignancies postoperatively.

Observations: A 7-year-old patient was admitted to the authors' hospital due to the in situ recurrence of a posterior fossa tumor more than 1 month after the initial surgery for headache.

The PDC30 Chatbot-Development of a Psychoeducational Resource on Dementia Caregiving Among Family Caregivers: Mixed Methods Acceptability Study.

Background: Providing ongoing support to the increasing number of caregivers as their needs change in the long-term course of dementia is a severe challenge to any health care system. Conversational artificial intelligence (AI) operating 24/7 may help to tackle this problem.

Objective: This study describes the development of a generative AI chatbot-the PDC30 Chatbot-and evaluates its acceptability in a mixed methods study.

Mandarin Chinese Translation and Cultural Adaptation of the Caregiver Analysis of Reported Experiences With Swallowing Disorders Screening Tool.

Purpose: The caregiver burden of individuals with dysphagia is a major concern. Currently, assessment tools specifically designed for this population are lacking. The present study aimed to translate the Caregiver Analysis of Reported Experiences with Swallowing Disorders (CARES) Questionnaire into Mandarin Chinese and evaluate its psychometric properties.

PHENYLALANINE AMMONIA-LYASE 2 regulates secondary metabolism and confers manganese tolerance in Stylosanthes guianensis.

Stylo (Stylosanthes guianensis) is a tropical legume that exhibits considerable tolerance to manganese (Mn) toxicity, which severely constrains plant growth in acidic soils. To elucidate the Mn detoxification mechanisms in stylo, this study investigated the excess Mn-regulated metabolic profile of stylo roots and examined the role of metabolic enzymes in Mn tolerance. Excess Mn triggered oxidative stress in the two stylo genotypes tested.

The NAC transcription factor LpNAC48 promotes trichome formation in Lilium pumilum.

Trichomes play a crucial role in plant resistance to abiotic and biotic stresses, and their development and characteristics vary across different species. This study demonstrates that trichomes of Lilium pumilum exhibit synchronized growth during flower bud differentiation and enhance the plant's adaptability to UV-B radiation and aphid infection. We identified LpNAC48, a NAC family transcription factor (TF), that interacted with the B-box (BBX) family TF LpBBX28, during trichome formation in L.

Development and psychometric properties of a quality of life assessment tool for Chinese patients undergoing intravitreal injection (IVI-QoL-23).

Purpose: To develop and assess the psychometric properties of a quality of life (QoL) assessment tool for Chinese patients treated with intravitreal injection (IVI) of anti-vascular endothelial growth factor (anti-VEGF).

Methods: We developed a 31-item IVI-QoL questionnaire using semi- structured patient interviews and expert panel consultation, drawing on a study of the literature. After pretesting on a subset of patients undergoing IVI, the questionnaire was pared down to 23 items.

Elevated plasma molybdenum level increases the risk of premature ovarian insufficiency through vascular endothelial injury.

Study Question: Is elevated plasma molybdenum level associated with increased risk for idiopathic premature ovarian insufficiency (POI)?

Summary Answer: Elevated plasma molybdenum level is associated with an increased risk of idiopathic POI through vascular endothelial injury and inhibition of granulosa cell proliferation.

What Is Known Already: Excessive molybdenum exposure has been associated with ovarian oxidative stress in animals but its role in the development of POI remains unknown.

Study Design, Size, Duration: Case-control study of 30 women with idiopathic POI and 31 controls enrolled from August 2018 to May 2019.

Assessing the Severity of Connective Tissue-Related Interstitial Lung Disease Using Computed Tomography Quantitative Analysis Parameters.

Objectives: The aims of the study are to predict lung function impairment in patients with connective tissue disease (CTD)-associated interstitial lung disease (ILD) through computed tomography (CT) quantitative analysis parameters based on CT deep learning model and density threshold method and to assess the severity of the disease in patients with CTD-ILD.

Methods: We retrospectively collected chest high-resolution CT images and pulmonary function test results from 105 patients with CTD-ILD between January 2021 and December 2023 (patients staged according to the gender-age-physiology [GAP] system), including 46 males and 59 females, with a median age of 64 years. Additionally, we selected 80 healthy controls (HCs) with matched sex and age, who showed no abnormalities in their chest high-resolution CT.

IDEAL-IQ Magnetic Resonance Imaging Fat Fraction Quantification in Distinguishing Thymic Hyperplasia From Low-Risk Thymoma and Thymic Lymphoma in Adulthood: A Reliability and Efficacy Analysis.

Objectives: Detection of fat content in thymic lesions is essential to differentiate thymic hyperplasia from thymic tumors. This study assesses the reliability and efficacy of "iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantization" IDEAL-IQ magnetic resonance sequence in distinguishing thymic hyperplasia from low-risk thymoma and thymic lymphoma in adulthood.

Methods: Thirty patients with thymic hyperplasia, 28 low-risk thymomas, and 13 thymic lymphomas were respectively enrolled.