UBE2C Mediates Follicular Thyroid Carcinoma Invasion and Metastasis via K29-Specific Vimentin Ubiquitination.

Follicular thyroid carcinoma (FTC) poses significant clinical challenges due to its vascular invasion tendency and distant metastasis potential, leading to poorer patient outcomes compared to other thyroid carcinomas. Although ubiquitin-conjugating enzyme E2C (UBE2C) has been widely studied in various cancers, its specific role in FTC progression remains insufficiently explored. This study demonstrates UBE2C's dual functionality in FTC through clinical analysis and experimental validation.

Innovative Construction and Application of Bile Duct Organoids: Unraveling the Complexity of Bile Duct Diseases and Potential Therapeutic Strategies.

The biliary system is crucial for liver function, regulating bile production, secretion, and transport. Dysfunctions within this system can lead to various diseases, such as cholangiopathies and biliary fibrosis, which may progress from benign to malignant states like cholangiocarcinoma. While liver organoid research is well-established and technologically advanced, bile duct organoids (BDOs) offer significant potential.

Fertility and Sterility top videos from 2022.

Objective: To objectively grade all video publications in Fertility & Sterility during the year 2022 and compile a list of the top 10 surgical videos.

Design: Descriptive presentation of the 10 highest scoring video publications from Fertility & Sterility in the year 2022.

Subjects: Not applicable EXPOSURE: JF, ZK, JPP, and SRL acted as independent reviewers of all video publications.

Higginsianin F, one skeletal rearrangement diterpenoid α-pyridone with phytotoxic activity isolated from Colletotrichum higginsianum.

Higginsianin F (1), a previously undescribed diterpenoid α-pyridone skeleton featuring a unique N-hydroxyethyl-3-(5-acetyl-4-hydroxy)-α-pyridone ring, along with three undescribed diterpenoid pyrones, higginsianins G-I (2-4), were isolated from the mycelium of the phytopathogenic fungus Colletotrichum higginsianum. Their structures were elucidated through comprehensive analysis using NMR, HRESIMS, and ECD experiments. The absolute configuration of compound 2 was determined to be opposite to the other three compounds based on ECD experiments and the measured optical rotation value.

Clerochinoids A‒H, aromatic abietane diterpenoids with cytotoxic activity from the roots and stems of Clerodendrum chinense.

Eight previously undescribed aromatic abietane diterpenoids, clerochinoids A-H (1-8), including seven 17(15→16)-abeo-abietanes (1-7) and one 17(15→16),18(4→3)-diabeo-abietane (8), along with 12 known analogues (9-20) were isolated from the roots and stems of Clerodendrum chinense. Their structures were elucidated by extensive spectroscopic data analysis, X-ray crystallography, and ECD calculations. All the compounds were screened for their cytotoxic activity against two human non-small cell lung cancer cell lines, H1975 and HCC827.

A pan-cancer analysis of small nucleolar RNA host gene 14 (SNHG14) in human tumors.

Long non-coding RNAs (lncRNAs) are associated with tumorigenesis and progression. One of these, short nucleolar RNA host gene 14 (SNHG14), has exhibited significant prognostic value due to its aberrant expression across various tumor types. This study investigates the expression patterns, survival outcomes, and tumor stages associated with SNHG14 across various cancers, employing data from the Genotype-Tissue Expression and The Cancer Genome Atlas databases.

12-month outcomes of pulsed-field ablation with a variable-diameter circular catheter for paroxysmal atrial fibrillation: Insights into conscious sedation and fluoroscopy use.

Background: Pulsed-field ablation (PFA) emerges as an innovative nonthermal energy modality for catheter ablation of atrial fibrillation (AF). This study aimed to assess the safety and effectiveness of a novel PFA system that uses a multichannel, circular ablation catheter with adjustable diameters in treating paroxysmal AF.

Methods: This clinical trial (PF-Beat-AF) was a prospective, multicenter, single-arm study.

Regulatory Frameworks and Filing Discrepancies in Generic Drug Approvals: A Cross-Regional Study with Analysis of FDA ANDA Deficiencies.

Objectives: This research aims to analyse the regulatory frameworks for generic drug applications in the US, EU, India, Japan, and China comparing their filing requirements to identify gaps and areas for harmonization. Additionally, it focuses on examining common deficiencies in ANDA from FDA submissions in 2014-2023 to address issues, facilitating more efficient approvals and minimizing delays.

Material And Methods: The research involved analysing regulatory documents available on official websites, including the FDA, EMA, CDSCO, PMDA, and NMPA to achieve first objective.

Maternal factors during pregnancy and pubertal timing in offspring: A systematic review of the literature.

Purpose: This systematic review aims to identify, assess the quality of and synthesize evidence on non-genetic maternal factors, such as psychological factors, lifestyle, nutrition, and endocrine conditions that may be associated with pubertal timing in male and female offspring.

Methods: The search was conducted in Medline, Embase, PsycInfo and Web of Science. The reference lists of retrieved articles were checked to avoid missing relevant studies.

Intracellular metabotropic glutamate receptor 5 in spinal dorsal horn neurons contributes to pain in a mouse model of vincristine-induced neuropathic pain.

Vincristine (VCR) is a commonly used clinical anti-cancer drug, but it can also induce neurotoxicity and cause vincristine-induced neuropathic pain (VINP). The metabotropic glutamate receptor 5 (mGluR5) within spinal dorsal horn neurons regulates the transmission of pain mediated by glutamate. In this study, we investigated for the first time the role of mGluR5 in the transmission of noxious information in VINP.

microRNA-34: A multifunctional miRNA family.

MicroRNAs are endogenous non-coding small RNAs composed of about 22 nucleotides, which are widely found in eukaryotic cells and regulate gene expression at the post-transcriptional level through complementary pairing with target genes, leading to mRNA degradation or translation inhibition. miR-34 is a highly conserved miRNA during evolution. Recent studies have found that members of the miR-34 family are involved in regulating biological processes such as aging, ciliogenesis, and immunity.

Research progress on critical viral protease inhibitors for coronaviruses and enteroviruses.

Viral infectious diseases have been seriously affecting human life and health. SARS-CoV-2 was the pathogen that caused Coronavirus Disease 2019 (COVID-19), and the impact of COVID-19 is still existing. Enterovirus 71 (EV71) is the primary pathogen of hand, foot, and mouth disease (HFMD), and no effective direct-acting antiviral drugs targeting EV71 has been approved yet.

Functional analysis of the epsilon glutathione S-transferases in the adaptation of Spodoptera litura to xanthotoxin.

Through long-term coevolution with host plants, insects have evolved sophisticated detoxification systems to counteract plant secondary metabolites (PSMs). However, the precise mechanisms underlying these adaptations remain incompletely characterized. Our previous research identified epsilon glutathione S-transferases (GSTes) as critical mediators of xanthotoxin adaptation in Spodoptera litura, a model linear furanocoumarin.

Epidemiology, Phylogeny and Genetic Characterization of Carbapenem-resistant Citrobacter spp. from 5 hospitals in China.

Objectives: Carbapenem-resistant Citrobacter spp. (CRC) are increasingly recognized as healthcare-associated pathogens, while systematic studies on clinical epidemiology, genetic diversity, and resistant mechanisms of CRC are relatively scarce. The present study provides comprehensive and systematic research on CRC.

Characterization of Klebsiella pneumoniae Carbapenemase (KPC)-14, a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in the Extensively Drug-resistant ST463 Pseudomonas aeruginosa Clinical Isolate.

Objectives: We studied two Klebsiella pneumoniae carbapenemase (KPC)-14 variants from clinical Pseudomonas aeruginosa isolates (C137 and C159) to better understand the genomic diversity, mechanisms, and genes that confer antibiotic resistance and pathogenicity.

Methods: Genomic DNA from C137/159 was subjected to Illumina and Oxford Nanopore sequencing. Horizontal transmission of the plasmid was evaluated using cloning experiments.

Meta-analyses of the global impact of non-antibiotic feed additives on livestock performance and health.

Introduction: The impact of non-antibiotic feed additives on livestock performance and health is contingent upon a multitude of variables, including the animal species, dosage and type of feed additives, and duration of oral administration. However, there is a paucity of knowledge regarding the relationship between these factors and the performance of livestock animals.

Objectives: The objective of this study was to conduct a global meta-analysis based on a pool of empirical studies to investigate the effects of dietary additives on growth, production, blood metabolites, immunity, intestinal morphology, and the abundance of gut microbiota in livestock.

Discovery and identification of semaphorin 4D as a bioindicator of high fracture incidence in type 2 diabetic mice with glucose control.

Introduction: Bone fracture is increasing in patients with type 2 diabetes mellitus (T2DM) due to skeletal fragility. Most antidiabetics are expected to reduce the incidence of fracture in patients with T2DM, however the results are disappointing. Metformin and GLP-1 receptor agonists have a neutral or minor positive effect in reducing fractures.

Reward prediction-error promotes the neural encoding of episodic learning.

Reward prediction-error carries significant implications for learning, facilitating the process by influencing prior knowledge and shaping future expectations and decisions. However, the electrophysiological mechanism through which reward prediction-error impacts learning remains incompletely understood. This study aimed to investigate the neural characteristics of reward prediction-error and its effect on recognition memory using Event-Related Potentials (ERPs).

Protective effects of anthocyanins on the nervous system injury caused by fluoride-induced endoplasmic reticulum stress in rats.

Long-term fluoride exposure can produce neurotoxicity. Anthocyanins, as antioxidants, have a certain protective effect in nerve damage. This study aimed to investigate the protective role of anthocyanins in fluoride-induced neurological damage due to endoplasmic reticulum stress (ERS).

20-Week Toxicity Study of Rotigotine Behenate Extended-Release Microspheres for Intramuscular Injection in Sprague Dawley Rats.

Based on the concept of continuous dopaminergic stimulation (CDS), Rotigotine Behenate extended-release microspheres for injection (RBEM) are currently under development. To support human clinical trials of RBEM, a 20-week repeat-dose toxicity study was conducted. SD rats intramuscularly received RBEM (60, 180, and 540 mg/kg) once every 4 weeks for 5 repeated doses followed by a 12-week recovery period, no clear sex difference was noted in the plasma exposure of rotigotine in rats, and the exposure generally increased in a dose-proportional manner.

Evaluating the reliability of geophysical methods for investigating the migration of a hydrocarbon plume: validation by sample analysis.

Geophysical methods are extensively used to assess contaminated sites. However, the validation of geophysical exploration results remains crucial for practical applications of these methods. In this study, Electrical Resistivity Tomography (ERT) and Ground Penetrating Radar (GPR) were used to investigate an abandoned hydrocarbon-contaminated site in Jiangsu, China.