A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii).

Fat greenling (Hexagrammos otakii Jordan & Starks, 1895) is a valuable marine fish species, crucial for aquaculture in Northern China due to its high-quality meat and significant economic value. However, the aquaculture industry faces challenges such as trait degradation, early sexual maturity, and disease susceptibility, necessitating advanced genomic interventions for sustainable cultivation. This study presents the first chromosomal-level genome assembly of H.

VEGFA, MYC, and JUN are abnormally elevated in the synovial tissue of patients with advanced osteoarthritis.

Osteoarthritis (OA), affecting > 500 million people worldwide, profoundly affects the quality of life and ability to work. The mitogen-activated protein kinase (MAPK) signaling pathway plays an essential role in OA. To address the lack of studies focused on synovial cells in OA, we evaluated the expression patterns and roles of the MAPK signaling pathway components in OA synovial tissues using bioinformatics.

Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae.

Neisseria gonorrhoeae is an on-going public health problem due in part to the lack of success with efforts to develop an efficacious vaccine to prevent this sexually transmitted infection. The gonococcal transferrin binding protein B (TbpB) is an attractive candidate vaccine antigen. However, it exhibits high levels of antigenic variability, posing a significant obstacle in evoking a broadly protective immune response.

Photoreceptor metabolic window unveils eye-body interactions.

Photoreceptors are specialized neurons at the core of the retina's functionality, with optical accessibility and exceptional sensitivity to systemic metabolic stresses. Here we show the ability of risk-free, in vivo photoreceptor assessment as a window into systemic health and identify shared metabolic underpinnings of photoreceptor degeneration and multisystem health outcomes. A thinner photoreceptor layer thickness is significantly associated with an increased risk of future mortality and 13 multisystem diseases, while systematic analyses of circulating metabolomics enable the identification of 109 photoreceptor-related metabolites, which in turn elevate or reduce the risk of these health outcomes.

Melanoma bone metastasis-induced osteocyte ferroptosis via the HIF1α-HMOX1 axis.

Osteocytes are the main cells in mineralized bone tissue. Elevated osteocyte apoptosis has been observed in lytic bone lesions of patients with multiple myeloma. However, their precise contribution to bone metastasis remains unclear.

Performance of Artificial Intelligence Chatbots on Ultrasound Examinations: Cross-Sectional Comparative Analysis.

Background: Artificial intelligence chatbots are being increasingly used for medical inquiries, particularly in the field of ultrasound medicine. However, their performance varies and is influenced by factors such as language, question type, and topic.

Objective: This study aimed to evaluate the performance of ChatGPT and ERNIE Bot in answering ultrasound-related medical examination questions, providing insights for users and developers.

ApoE Genotype, Age, and Cognitive Decline in Old Chinese Individuals Without Dementia: A Population-Based Study With Five-Year Follow-Up.

Background: Apolipoprotein E (ApoE) ε4 genotype is a well-known risk factor for Alzheimer's disease (AD). However, its effect on predicting cognitive decline in individuals without dementia and its association with age are unclear.

Objective: To investigate the relationship between ApoE polymorphism and risk of cognitive decline and dementia incidence in the elderly without dementia.

Adjusting TADF and Phosphorescence for Tailored Dynamic Time-Dependent Afterglow Colored Carbon Dots spanning Full Visible Region.

Time-dependent afterglow colored (TDAC) behavior differs from static afterglow by involving wavelength changes, enabling low-cost, high-level encryption and anti-counterfeiting. However, the existing carbon dot (CD)-based TDAC materials lack a clear mechanistic explanation and controllable wavelength changes, significantly hindering the progress of practical applications in this field. In this study, we synthesized CDs composites with customizable tunable TDAC wavelengths across the visible region.

Natural Kaurane-class of Diterpenoid Alkaloids: Structure, Distribution, and Bioactivities.

Kaurane-class diterpenoid alkaloids (DAs), an important group of C20-DAs, include mainly veatchine-, napelline-, anopterine- and tricalysiamide-type DAs and several types of DAs with novel skeletons discovered in recent years. To date, approximately 81 compounds belonging to this class of DAs have been isolated from plants in 5 families and 7 genera. Among them, Aconitum is the most important source of this class of DAs.

Intramolecular DNA Wheel Construction for Highly Sensitive Electrochemical Detection of miRNA.

Accurate and reliable quantification of disease-related biomolecules is essential for clinical diagnosis. In this study, a novel electrochemical approach is developed based on a target triggered DNA nanostructural switch from a hairpin dimer to a double-stranded wheel. During the process, electrochemical species get closer to the electrode interface, and the multiple intramolecular strand displacements are beneficial to low abundant target analysis.

A Multimodal Model Based on Transvaginal Ultrasound-Based Radiomics to Predict the Risk of Peritoneal Metastasis in Ovarian Cancer: A Multicenter Study.

Rationale And Objectives: This study aimed to develop a predictive model for peritoneal metastasis (PM) in ovarian cancer using a combination radiomics and clinical biomarkers to improve diagnostic accuracy.

Patients And Methods: This retrospective cohort study of 619 ovarian cancer patients involved demographic data, radiomics, O-RADS standardized description, clinical biomarkers, and histological findings. Radiomics features were extracted using 3D Slicer and Pyradiomics, with selective feature extraction using Least Absolute Shrinkage and Selection Operator regression.

Cutting through the noise: A narrative review of Alzheimer's disease plasma biomarkers for routine clinical use.

As novel, anti-amyloid therapies have become more widely available, access to timely and accurate diagnosis has become integral to ensuring optimal treatment of patients with early-stage Alzheimer's disease (AD). Plasma biomarkers are a promising tool for identifying AD pathology; however, several technical and clinical factors need to be considered prior to their implementation in routine clinical use. Given the rapid pace of advancements in the field and the wide array of available biomarkers and tests, this review aims to summarize these considerations, evaluate available platforms, and discuss the steps needed to bring plasma biomarker testing to the clinic.

Effects of axillary dose restriction on plan quality and acute radiation dermatitis in breast cancer radiotherapy.

Axillary dose restriction (ADR) is rarely implemented in breast cancer radiotherapy by radiation oncologists to minimize exposure to organs at risk (OARs), particularly the axilla. This prospective randomized controlled study aims to evaluate the efficacy of ADR in improving plan quality (PQ) and its impact on acute radiation dermatitis (ARD) in breast cancer radiotherapy. The study recruited breast cancer patients who required postoperative radiotherapy but did not have an indication for axillary irradiation.

A nomogram for predicting survival in patients with primary testicular lymphoma: A population-based study.

Background: Primary testicular lymphoma (PTL) is a rare malignancy whose epidemiology and prognosis have not been studied.

Materials And Methods: PTL patient data were collected from the SEER online database, and the data were divided into a training cohort and a validation cohort according to random assignment. The training cohort was subjected to a one-way COX regression analysis, and statistically significant differences were included in the multi-factor COX regression analysis and constructed nomograms.

TPPP3, a Good Prognostic Indicator, Suppresses Cell Proliferation and Migration in OSCC.

Introduction And Aims: Oral squamous cell carcinoma (OSCC) is one of the most prevalent malignancy of the head and neck. Early diagnosis of OSCC is difficult and the prognosis has not improved significantly. This study aims to explore the role of tubulin polymerisation promoting protein 3 (TPPP3) in the occurrence and development of OSCC and discover new diagnostic and prognostic markers for OSCC.

Efficacy of Acupuncture in Postoperative Pain-Relieving: A Systematic Review and Meta-Analysis.

Objectives: This study aimed to assess the efficacy of acupuncture in relieving postoperative pain.

Design: Systematic review and meta-analysis.

Data Sources: The search strategy was designed according to the PICOS principle (population, intervention, comparison, outcome, and study).

Vonoprazan Fumarate: A Promising Therapeutic Option for the Rapid Relief of Laryngopharyngeal Reflux Disease.

Objective: The aim is to examine the Reflux Symptom Score-12 (RSS-12) and assess the effectiveness of vonoprazan fumarate in managing laryngopharyngeal reflux disease (LPRD) among the Chinese population.

Methods: A total of 140 patients with LPRD who were treated at our otorhinolaryngology outpatient clinic were included. The patients were randomly divided into the vonoprazan treatment group and the esomeprazole treatment group.

Secure synchronization control for complex dynamic networks with event-triggered communication strategy under multi-channel denial-of-service attacks.

This article addresses the secure synchronization problem for complex dynamical networks (CDNs) with observer-based event-triggered communication strategy (ETCS) under multi-channel denial-of-service attacks (MCDSAs). Due to external environmental interference, the observers are designed to accurately estimate the state of the network systems. Meanwhile, the impact of cyber attacks on system security is considered.

Fluorescent Macrocyclic Arenes: Synthesis and Applications.

Fluorescent macrocyclic arenes have attracted increasing interest in macrocyclic and supramolecular chemistry due to their exceptional photophysical properties and versatile applications. Classical macrocyclic arenes modified with fluorescent groups at the upper or bottom rims have long provided valuable platforms across various fields. Recently, a large number of novel fluorescent macrocyclic arenes directly composed of polycyclic aromatic or heteroaromatic building blocks including naphthalene, anthracene, tetraphenylethene, pyrene, fluorene, carbazole, acridan, phenothiazine, coumarin,  triphenylamine, benzothiadiazole and so on, have been reported, and they have shown specific fluorescent property, and also exhibited broad applications in molecular recognition, sensing, bioimaging and functional materials.

Exploring the Maximum Potential of Initiating Ability in Metal-Free Primary Explosives.

Lead azide (LA) is a widely utilized primary explosive, serving as the initiating charge in blasting caps or detonators to start the detonation process of secondary explosives. The toxicity and environmental concerns associated with LA have led to regulatory restrictions and increased scrutiny, prompting the search for lead-free alternatives. LA is highly sensitive toward heat, shock, or friction, which poses safety challenges during manufacturing, handling, and storage.

VPS13D-related disorders: a severe case, review, and genotype-phenotype correlation.

Background: -related disorders are autosomal recessive genetic disorders characterized by movement disorders primarily including ataxia and spasticity, mainly accompanying developmental delay, seizures, and neuroimaging abnormalities. -related spectrum disorder (VSD) may better reflect the characteristics of the disease. So far, the relationship of genotype and phenotype of VSD has not been established.

Association between Mediterranean diet and metabolic syndrome: analysis of NHANES 2007-2020.

Changing poor dietary habits is effective for treating metabolic syndrome (MetS). Despite the global reputation of the Mediterranean diet (MD) for health, research on its link to MetS is limited, especially in non-Mediterranean regions. This study aimed to investigate the relationship between the MD and MetS.

Do wood-boring beetles influence the flammability of deadwood?

Global warming increases the risk of wildfire and insect outbreaks, potentially reducing the carbon storage function of coarse woody debris (CWD). There is an increasing focus on the interactive effects of wildfire and insect infestation on forest carbon, but the impact of wood-boring beetle tunnels via their effect on the flammability of deadwood remains unexplored. We hypothesized that the presence of beetle holes, at natural densities, can affect its flammability positively through increased surface area and enhanced oxygen availability in the wood.

Ppb-Level Photoacoustic Detection of Chloroform Using Four-Microphone Array.

The photoacoustic spectroscopy (PAS) system commonly enhances the efficiency of optical-acoustic-electrical energy conversion by increasing the laser power, optimizing the resonance characteristics of the photoacoustic cell (PAC), and improving the sensitivity of acoustic sensors. However, conventional systems using a single-microphone or a dual-microphone differential setup for point sampling of the photoacoustic signal fail to account for its spatial distribution, leading to a loss of spatial gain. Drawing on microphone array theory derived from sonar technology, this study, for the first time, presents a PAS sensing system based on a four-microphone array, which is applied to detect chloroform gas.

In Utero Perfluorodecanoic Acid Exposure Causes Fetal Leydig Cell Dysfunction via Endoplasmic Reticulum Stress-Mediated Lipid Composition Alteration.

Perfluorodecanoic acid (PFDA), a C10 fluorine-containing compound, is used widely and found to be present anywhere. However, whether it has reproductive toxicity for fetal Leydig cells and the underlying mechanisms remain unknown. PFDA was investigated for its effects on fetal Leydig cells (FLCs) following exposure to 0, 1, 2.