Mechanosensitive Ca channel TRPV1 activated by low-intensity pulsed ultrasound ameliorates acute kidney injury through Notch1-Akt-eNOS signaling.

Acute Kidney Injury (AKI) is a significant medical condition characterized by the abrupt decline in kidney function.Low-intensity pulsed ultrasound (LIPUS), a non-invasive therapeutic technique employing low-intensity acoustic wave pulses, has shown promise in promoting tissue repair and regeneration. A novel LIPUS system was developed and evaluated in rat AKI models, focusing on its effects on glomerular filtration rate (GFR), blood urea nitrogen (BUN), serum creatinine (SCr), and the Notch1-Akt-eNOS signaling pathway.

Biomarkers.

Background: Type 2 diabetes mellitus (T2DM) is associated with a greater risk of Alzheimer's disease (AD). Synaptic impairment and protein aggregates have been reported in the brains of T2DM rodent models. Here, we assessed the changes in synaptic vesicle 2A (SV2A), amyloid-β, and tau that are featured pathologies in AD in T2DM rats in vivo.

Targeting pancreatic cancer cell stemness by blocking fibronectin-binding integrins on cancer-associated fibroblasts.

Cancer-associated fibroblasts (CAF) generate an extracellular matrix (ECM) which provides a repository for factors that promote pancreatic cancer progression. Here, we establish that CAF contribution to pancreatic tumor initiation, i.e.

sp. nov., sp. nov. and sp. nov. isolated from the phyllosphere of litchi.

Three aerobic, pink-pigmented, Gram-negative, motile and rod-shaped bacterial strains, designated SD21, SI9 and SB2, were isolated from the phyllosphere of healthy litchis collected from three main producing sites of Guangdong Province, PR China. The 16S rRNA gene analysis showed that strains SD21 and SI9 belonged to the genus (.) with the highest similarity to DSM 19563 (98.

Undernutrition affects metabolism and immune response in subcutaneous adipose tissue of pregnant ewes.

Pregnant ewes mobilize body fat to increase energy supply for fetal growth and development upon undernutrition, which disrupts the metabolic homeostasis of the body. However, the comprehensive metabolic changes in subcutaneous adipose tissue upon undernutrition are poorly understood. In this study, an undernutrition sheep model was established to investigate the effects of undernutrition on metabolic changes, immune response, and inflammation in subcutaneous fat through transcriptome, RT-qPCR, and metabolome analysis.

Biomarkers.

Background: The driving mechanisms of structural brain alterations in the earliest stages of Alzheimer's disease (AD) are not well understood. Previous heterogeneous findings in preclinical AD, including subtle atrophy and also increased grey matter (GM) volume, underscore the need for further exploration. This study uses an extensive fluid biomarkers panel to identify pathological drivers behind longitudinal GM changes in cognitively unimpaired (CU) adults.

Biomarkers.

Background: Cognitive Reserve (CR) refers to the brain's ability to maintain optimal cognitive function despite damage or pathology. The neural implementation of CR is a major research focus, and resting-state functional connectivity (RSFC) has emerged as a promising imaging correlate of CR. We assessed RSFC as a function of two different proxy measures of CR and further assessed the impact of these brain networks on longitudinal cognitive performance in a sample of cognitively unimpaired (CU) individuals at risk of Alzheimer's disease (AD).

Biomarkers.

Background: Synaptic dysfunction plays an important role in Alzheimer's disease (AD) and cognitive decline. We investigated the association between cerebrospinal fluid (CSF) synaptic protein levels and quantitative EEG (qEEG) measures, two modalities to measure synaptic dysfunction in AD pathology. We assessed combined and independent prognostic value of both modalities for cognitive decline along the AD continuum.

Biomarkers.

Background: Mild cognitive impairment (MCI) refers to a transitional stage between the cognitively unimpaired (CU) and dementia, which is probable in the Alzheimer's disease (AD) continuum. Neuropsychological assessments and structural MRI are primary clinical examinations applied for diagnosing MCI. Hippocampal volume (HV) and medial temporal lobe atrophy (MTA) as recognized AD biomarkers are the most frequently used imaging markers in clinical application.

Transferrin Modified Gold Nanoclusters-Based Biosensing Nanoplatform for High-Precision Multimodal Bioimaging of Tumor Cells.

Bioimaging technology has been broadly used in biomedicine, and the growth of multimodal imaging technology based on synergistic advantages can overcome the shortcomings of traditional single-modal bioimaging methods and attain high specificity and sensitivity in the fields of bioimaging and biosensing. The analysis of low-abundance microRNAs (miRNAs) in complex organisms is of high importance for early-stage diagnosis and clinical treatment of tumors. In our current study, a biosensing nanoplatform based on Tf-AuNCs and MnO nanosheets was developed for multimodal imaging of tumor cells.

Biomarkers.

Background: Alzheimer's disease (AD) is the most common form of dementia, characterized by cognitive impairment and memory loss. Previous studies have demonstrated that plasminogen, a key molecule in the fibrinolytic system, is implicated in the pathophysiology of AD. However, it is yet unknown whether the relationship between blood plasminogen and AD is causal.

Biomarkers.

Background: It is challenging to predict which patients who meet criteria for subcor- tical ischemic vascular disease (SIVD) will ultimately progress to subcortical vascular cognitive impairment (SVCI).

Method: We collected clinical information, neuropsychological assessments, T1 imag- ing, diffusion tensor imaging, and resting-state functional magnetic resonance imaging from 83 patients with SVCI and 53 age-matched patients with SIVD without cogni- tive impairment. We built an unsupervised machine learning model to isolate patients with SVCI.

Biomarkers.

Background: The objectives of the study is to assess the clinical utility of the MemTrax Memory Test for detection of cognitive impairment in patients with PD.

Method: The MemTrax and Montreal Cognitive Assessment (MoCA) were administered to 61 healthy controls (HC), 102 PD patients with normal cognition (PD-N), 74 PD patients with mild cognitive impairment (PD-MCI) and 52 PD patients with dementia (PD-D). The MemTrax performance, MTx-%C, MTx-RT and MTx-Cp, and the MoCA scores were comparatively analyzed.

Biomarkers.

Background: Vascular cognitive impairment (VCI), including vascular cognitive impairment-no dementia (VCIND) and vascular dementia, is a cognitive impairment syndrome caused by cerebrovascular disease and its risk factors. Among people over 60 years old, the prevalence of VCIND is about 15-20%. VCIND, as the early stage of VCI, has become a focus of current research due to the fact that its patients are at greater risk of developing dementia and can benefit greatly from early intervention.

Biomarkers.

Background: The immune complement system is key to the elimination of redundant neural connections in the brain through a process called synaptic pruning. In neurodegenerative diseases such as Alzheimer's disease (AD), this system may result in excessive synapse loss, leading to brain atrophy and cognitive impairment. While increased cerebrospinal fluid (CSF) levels of complement proteins have been observed in patients with AD dementia, no studies have yet investigated the role of complement in the pre-symptomatic phase of AD, nor throughout its progression.

Biomarkers.

Background: Co-pathology between Alzheimer's disease (AD), Parkinson's disease (PD), and dementia with Lewy bodies (DLB) remains poorly understood but is relevant for trial design. We aimed to compare CSF markers of amyloid, tau, and neurodegeneration (ATN) and α-synuclein between AD, PD, DLB and controls, and investigate the influence of demographical, genetic, and clinical factors on amyloid positivity.

Method: As part of the EPND study, we included 337 individuals with AD, PD, DLB and controls from 6 centers.

Biomarkers.

Background: Microstructural brain lesions have been linked to cognitive health. However, their combined effect and the role of the cognitive reserve (CR) remain unclear. We investigated the interplay of microstructural brain lesions and cognitive reserve in cognitive function in middle-aged and elderly adults.

β-Thioamide Sulfone Enabled Copper-Catalyzed Ring-Opening/Sulfonylation of Cyclopropenes: Access to Alkyl Aryl Sulfones.

Sulfone motifs play important roles in bioactive compounds and functional materials. The development of efficient methodologies for constructing sulfonyl-containing compounds has thus attracted considerable attention. Here, we introduce a protocol for the preparation of alkyl aryl sulfones under mild conditions.

Biomarkers.

Background: Alzheimer's disease (AD) is a devastating neurodegenerative disease with delayed diagnosis until the manifestation of symptoms. Although the emergence of blood-based biomarkers offers hope for easy detection of AD, existing AD-associated blood biomarkers, known as the "blood ATN biomarkers", mainly capture the pathological hallmarks of AD, overlooking other AD-associated biological processes such as inflammation and vascular dysfunctions. Therefore, developing a blood-based biomarker assay that captures dysregulation beyond the ATN biomarkers may help advance early detection and staging of AD, enabling a comprehensive examination of the disease status METHOD: We leveraged ultrasensitive proteomic technology to develop a blood-based, multiplex biomarker assay for AD.

Targeting WDR5/ATAD2 signaling by the CK2/Ikaros axis demonstrates therapeutic efficacy in T-ALL.

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with a poor prognosis and limited options for targeted therapies. Identifying new molecular targets to develop novel therapeutic strategies is the pressing immediate issue in T-ALL. Here, we observed high expression of WD Repeat-Containing Protein 5 (WDR5) in T-ALL; with in vitro and in vivo models we demonstrated the oncogenic role of WDR5 in T-ALL by activating cell cycle signaling through its new downstream effector, ATPase family AAA domain-containing 2 (ATAD2).

Novel robust control with disturbance rejection for permanent magnet synchronous motors and experimental validation.

A novel robust control strategy is proposed in this work to address the dynamic control problem of permanent magnet synchronous motors (PMSM) position tracking and lessen the effect of system parameter and load fluctuations on the dynamic performance of PMSM. The tracking performance is improved by a robust control element built with the Lyapunov method to reduce the impact of uncertain factors such as parameter uncertainty, nonlinear friction, and external interference; the nominal control element is stabilized by the dynamics model. The uniformly bounded and uniformly final bounded systems are proven, and the associated conclusions are provided using the Lyapunov minimax approach.

Investigation of a flat-type piezoelectric motor using in-plane vibrations.

This paper presents a flat-type piezoelectric motor utilizing in-plane vibration modes. Two piezoelectric ceramic plates in combination with a brass metal sheet were used to construct the stator. The superposition of two second order in-plane vibration modes can generate a traveling-wave inside the stator.

Simulation of high signal-to-noise ratio resonant photodetector for homodyne measurement and its verification.

In this paper, two models for simulating the shot noise and electronic noise performances of resonant photodetectors designed for homodyne measurements are presented. One is based on a combination of a buffer and a low-noise amplifier, and the other is based on an operational amplifier. Through the comparisons between the numerical simulation results and the experimentally obtained data, excellent agreements are achieved, which show that the models provide a highly efficient guide for the development of a high signal-to-noise ratio (SNR) resonant photodetector.

Biomarkers.

Background: Studies on plasma biomarkers have shown promising results for Alzheimer's disease(AD) neuropathology in a research setting, but their associations to neuropsychological(NP) tests have not been extensively studied in clinical cohorts. The main aim was to investigate associations between NP, plasma biomarkers and amyloid accumulation in the brain(Aβ-PET).

Method: We quantified plasma biomarkers (tau phosphorylated at 217, 181 and 231, glial fibrillary acidic protein(GFAP) and neurofilament-light(NFL)) by single molecular array in 126 patients (age=65±8) who were admitted to the Clinic for Cognitive Disorders, Karolinska University Hospital.

Biomarkers.

Background: Alzheimer´s disease (AD) is the main cause of death in adults with Down syndrome (DS). We describe the unique contributions of the Down Alzheimer Barcelona Neuroimaging Initiative (DABNI) cohort by studying longitudinal changes in plasma and cerebrospinal fluid (CSF) markers.

Method: We included DABNI participants with DS that contributed at least two plasma and/or CSF samples and were asymptomatic (aDS, n=155), had prodromal AD (pDS, n=46) or had AD dementia (dDS; n=53) at baseline, together with 172 euploid cognitively normal controls (CN).