Jatrorrhizine retard obesity by modulating transcription factor c-Jun/c-Fos to downregulate Mmp12-mediated inflammation.

Obesity is a systemic, chronic, low-grade inflammatory disease. Nutritional obesity, in particular, is also accompanied by inflammation and metabolic disorders, which are the primary causes of malignant metabolic diseases. Rhizoma Coptidis (Coptis Chinensis Franch) (RC), a traditional Chinese medicine, is primarily used for its anti-inflammatory and anti-diarrheal properties.

Development and validation of a HPLC-MS/MS method the determination of genistein and equol in serum, urine and follicular fluid.

Soy isoflavones exert estrogen-like synergistic or antagonistic effects by binding to estrogen receptors, and potentially impact the function of female reproductive system, but their distribution profile in human remains little clarified. To determination of genistein (GEN) and equol (EQ) in human urine, serum and follicular fluid (FF), an analytical method based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. The enrichment and clean-up are performed on a solid-phase extraction (SPE) column; the elution is a gradient one, with the mobile phase (A) of 0.

Yi-qi-yang-yin decoction ameliorates diabetic retinopathy: New and comprehensive evidence from network pharmacology, machine learning, molecular docking and molecular biology experiment.

Yi-Qi-Yang-Yin Decoction (YQYY), a traditional Chinese medicine (TCM) formula, has been used to treat diabetic retinopathy (DR), yet its precise mechanisms of action remain poorly understood. In this study, two distinct diabetic models, namely spontaneous type 2 diabetic db/db mice and streptozotocin (STZ)-induced type 1 diabetic rats, were employed to assess the efficacy of YQYY in ameliorating DR. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was employed to identify the chemical composition of YQYY in mouse serum.

Importance of intestinal microflora: Dried toad skin-radix clematidis plasma component analysis and anti-CRC core target study.

The focus of this study is to explore the impact of gut microbiota in different states on the blood components of couplet medications (dried toad skin and radix clematidis) and to identify drug metabolites associated with the gut microbiota. By constructing a pseudo-sterile rat model and combining non-targeted metabolomics with plasma pharmacology, we found that the plasma metabolites of couplet medications underwent significant changes in different gut microbiome environments. The GABA and PGE1 levels in the model group and the model+TCM (traditional chinese medicine) group were both significantly lower than those in the normal+TCM group.

Efficacy and safety of entrectinib in children with extracranial solid or central nervous system (CNS) tumours harbouring NTRK or ROS1 fusions.

Background: Entrectinib, a central nervous system (CNS)-penetrant TRK/ROS1 inhibitor, has demonstrated clinical activity in children with NTRK1/2/3 or ROS1 fusion-positive extracranial solid and CNS tumours. We present integrated data of entrectinib in children with NTRK or ROS1 fusion-positive tumours from the STARTRK-NG, TAPISTRY, and STARTRK-2 trials.

Methods: Efficacy analyses were undertaken on TRK/ROS1 inhibitor-naïve patients aged <18 years with metastatic/locally advanced NTRK1/2/3 or ROS1 fusion-positive extracranial solid or CNS tumours who received ≥1 entrectinib dose and had ≥6 months of follow-up from enrolment.

Two-year follow-up of a rare primary branchial cleft carcinoma: Case analysis and management insights.

Branchial cleft cysts are congenital anomalies of development, with the second branchial cleft cyst being the most common clinically. In extremely rare cases, branchial cleft cysts can undergo malignant transformation. Herein, we present a rare case of a 65-year-old male patient who had a painless neck mass on the left side for 6 months.

The 100 most-cited radiomics articles in cancer research: A bibliometric analysis.

Radiomics, an advanced medical imaging analysis technique introduced by Professor Lambin in 2012, has quickly become a key area of medical research. To explore emerging trends in cancer-related radiomics, we conducted a bibliometric analysis of the 100 most-cited articles (T100) in this field. Data were collected from the Web of Science Core Collection on October 7, 2023, and the articles were ranked by citation count.

Long-term low-level ozone exposure and the incidence of type 2 diabetes mellitus and glycemic levels: A prospective cohort study from Southwest China.

Background: This study investigated the relationship between long-term low-level ozone (O) exposure, type 2 diabetes mellitus (T2DM) incidence, and glycemic levels within a prospective cohort in Southwest China, especially in regions with relatively low air pollution levels.

Method: Between 2010 and 2020, the Guizhou Population Health Cohort Study (GPHCS) enrolled 9280 participants, who were followed up from 2016 to 2020. A total of 7317 participants (aged 18-95 years, mean 43.

Occurrence, formation mechanism, and health risk of polycyclic aromatic hydrocarbons in barbecued food.

Polycyclic aromatic hydrocarbons (PAHs) show negative impacts on human health. Dietary intake is the predominant way for PAH exposure, of which barbecued food is a crucial contributor. This review aims to provide a comprehensive insight into the formation mechanism, influencing factors, mitigation strategies, and health risks of PAHs in barbecued food.

Edgeworthia gardneri (Wall.) Meisn Mitigates CCL4-induced liver injury in mice by modulating gut microbiota, boosting antioxidant defense, and reducing inflammation.

Herbal medicine has become an area of growing global scientific interest. The prime objective of this study was to investigate the protective role of Edgeworthia gardneri (Wall.) Meisn (EGM polysaccharide) against carbon tetrachloride (CCl)-induced liver injury in mice.

Integration analysis of miRNA-mRNA uncovers the mechanisms of ochratoxin A-induced hepatotoxicity.

Ochratoxin A (OTA), the most toxic member of the ochratoxin family, is frequently detected in contaminated food and beverages, posing substantial health risks to both humans and animals, particularly due to its hepatotoxic effects. Although OTA is known to cause liver damage, the precise molecular mechanisms driving its toxicity remain poorly understood. In this study, we explored the hepatotoxic effects of OTA using LO2 cells and zebrafish models, combining miRNA and mRNA analyses to uncover the underlying mechanisms.

Transcriptomic and metabolic profiling reveals the effects of long-term microwave exposure on testicular tissue.

The effect of electromagnetic exposure on health is becoming increasingly important as it affects many aspects of human life and health. However, the effects in environmental electromagnetic fields on the male reproductive system were still controversial, and the impacts of long-term microwave exposure on testicular tissue remain poorly defined. This study exposed rats to 30 mW/cm of microwave radiation (2.

Providing an alternative explanation improves misinformation rejection and alters event-related potentials during veracity judgements.

The continued influence effect of misinformation (CIE) occurs when misinformation affects memory and decision making even after correction. Here, we examined the neurocognitive processes underlying the correction and subsequent veracity judgements of misinformation. Employing electroencephalography (EEG), we examined event-related potentials (ERPs): the P300 during encoding of corrections, and the P300 and FN400 during subsequent veracity judgement.

Gestational age at birth and cognitive outcomes in term-born children: Evidence from Chinese and British cohorts.

Introduction: Older gestational age (GA) has been associated with more favourable cognitive outcomes in preterm children. Recent evidence suggests this may also apply to term-born children. This study aims to examine the association between GA and early neurodevelopmental outcomes in children born at term in China and the UK.

Smart hydrogel: A new platform for cancer therapy.

Cancer is a significant contributor to mortality worldwide, posing a significant threat to human life and health. The unique bioactivity, ability to precisely control drug release, and minimally invasive properties of hydrogels are indispensable attributes that facilitate optimal performance in cancer therapy. However, conventional hydrogels lack the ability to dynamically respond to changes in the surrounding environment, withstand drastic changes in the microenvironment, and trigger drug release on demand.

NHANES-based machine learning for cognitive impairment classification and blood and hearing threshold characterization in age-related hearing loss.

Objective: This study aims to develop a machine learning-based classification model for cognitive impairment (CI) in elderly deaf patients and analyze the contributions of blood indices and hearing characteristics in identifying CI.

Methods: Blood and audiometric data from 833 elderly deaf patients across three NHANES cycles were used to build a classification model with five algorithms: Logistic Regression, Random Forest (RF), XGBoost, Artificial Neural Networks (ANN), and Support Vector Machine (SVM). The optimal model was selected to rank feature importance.

Extracellular vesicles engineered to directly target encephalomyocarditis virus ameliorates multi-organ viremia in a lethal infection model.

The outbreak and prevalence of encephalomyocarditis virus (EMCV) causes significant global mortality and morbidity to the pig industry. Though the current and most effective approach to control EMCV outbreak are done through inactivated vaccines, we have yet to see an effective antiviral agent that directly targets EMCV. Here, we present a molecular therapy consisting of extracellular vesicles (EVs) decorated with EMCV-specific single-chain variable fragment (scFv), engineered on the external loop of the EVS transmembrane domain CD63.

Ellagic acid ameliorates atherosclerosis by inhibiting PCSK9 through the modulation of FoxO3 and HNF1α.

Introduction: Proprotein convertase subtilisin/kexin type 9 (PCSK9) hinders the clearance of low-density lipoprotein cholesterol (LDL-C) by promoting the degradation of the low-density lipoprotein receptor (LDLR), leading to the accumulation of LDL-C and thus becoming an important cause of atherosclerosis. Ellagic acid, a naturally occurring polyphenol widely present in fruits, vegetables, and nuts, has attracted significant attention due to its potential role in the prevention and treatment of cardiovascular diseases. However, the molecular mechanisms by which ellagic acid alleviates atherosclerosis by inhibiting PCSK9 are not fully understood.

Advances of novel hydrogels in the healing process of alveolar sockets.

Tooth extraction is a common oral surgical procedure that often leads to delayed alveolar socket healing due to the complexity of the oral microenvironment, which can hinder the patient's aesthetic and functional recovery. Effective alveolar socket healing requires a multidisciplinary approach. Recent advancements in materials science and bioengineering have facilitated the development of innovative strategies, with hydrogels emerging as ideal restorative materials for alveolar socket repair due to their superior properties.

Impact of adjustable swelling dynamics on the structural integrity of sunflower pollen microgels.

Pollen is a renewable biomaterial found in seed-bearing plants, and the biocompatibility of pollen microgels is a key factor driving their use in drug delivery, biosensor development, and wound care applications. Herein, the microgel was synthesized from sunflower bee pollen by using a cost-effective process, and to examine its structural integrity under adverse acidic and alkaline conditions, digital microscopy and dynamic image particle analysis were carried out. Swelling dynamics of pollen microgels were regulated by varying pH conditions, and adding aqueous KOH to the solution, and the influence of swelling-deswelling on bulk rheology and local elastic properties were experimentally investigated and theoretically interpreted by using the Ross-Minton equation for the suspension viscosity.

V-domain Ig Suppressor of T cell Activation Expression During Hemorrhage or Sepsis-Induced Acute Respiratory Distress Syndrome: Insights From a Mouse Model.

Introduction: Acute respiratory distress syndrome (ARDS) is a life-threatening pulmonary condition with significant mortality, largely due to a lack of therapeutic interventions grounded in its molecular pathophysiology. Immune checkpoint regulators, such as the V-domain Ig Suppressor of T cell Activation (VISTA), may provide novel immunotherapeutic strategies for ARDS by modulating the immune response, a concept extensively explored in cancer and autoimmune diseases. Investigating VISTA in the context of ARDS could unveil new therapeutic avenues.

The roles of algal polysaccharides in modulating tumor immune microenvironment.

Background: Polysaccharides from algae provide a range of biology and health benefits. Lately, there has been a significant interest in how algal polysaccharides affect the immune microenvironment around tumors.

Purpose: To elucidate the subtle interactions between algal polysaccharides and the tumor immune microenvironment to further understand the medicinal potential of algal polysaccharides.

Metabolomics based analysis reveals the therapeutic effects of Incarvillea arguta (Royle) Royle aqueous extract against alcohol-induced liver injury.

Background: Alcohol-induced liver injury (ALI) poses a significant threat to global human health. The Chinese Yi medicine Liangtoumao (LTM), which originated from the whole plant of Incarvillea arguta Royle (Royle), has been widely used by the Yi ethnic group to prevent and treat ALI and other liver diseases. However, its effectiveness and mechanisms are still under-researched.

Neuroprotective role of geniposide-loaded UMSC nanovesicles in depression via P2ry12 downregulation.

Background: Depression is a prevalent mental disorder characterized by persistent low mood, loss of interest, and cognitive impairment. Oxidative stress and inflammation play crucial roles in its pathogenesis. Novel therapeutic strategies targeting these mechanisms are needed to improve treatment outcomes.