[Effect of Bifidobacterium on the maturation and function of dendritic cell originating from blood monocyte].

Objective: To study in vitro whether the bifidobacterium plays a role in the functioning of dendritic cell (DC) stimulating the proliferation of T lymphocyte, and has effect on the generation of cytokine secreted by the DC deriving from the normal adult peripheral blood monocyte.

Methods: Normal adult immature DCs were generated in induction from monocyte cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). On the 7th day of cell culture, immature DCs were exposed to various dosage heat-killed bifidobacteria (100 microg/mL, 10 microg/mL), while the control group was exposed to the culture without heat-killed bifidobacterrium, LPS at 1 microg/mL was added to some cultures as positive control.