Programming scheduled self-assembly of circadian materials.

Active biological molecules present a powerful, yet largely untapped, opportunity to impart autonomous regulation of materials. Because these systems can function robustly to regulate when and where chemical reactions occur, they have the ability to bring complex, life-like behavior to synthetic materials. Here, we achieve this design feat by using functionalized circadian clock proteins, KaiB and KaiC, to engineer time-dependent crosslinking of colloids.

Transition metal vacancy and position engineering enables reversible anionic redox reaction for sodium storage.

Triggering the anionic redox reaction is an effective approach to boost the capacity of layered transition metal (TM) oxides. However, the irreversible oxygen release and structural deterioration at high voltage remain conundrums. Herein, a strategy for Mg ion and vacancy dual doping with partial TM ions pinned in the Na layers is developed to improve both the reversibility of anionic redox reaction and structural stability of layered oxides.

Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy.

Optical aberrations hinder fluorescence microscopy of thick samples, reducing image signal, contrast, and resolution. Here we introduce a deep learning-based strategy for aberration compensation, improving image quality without slowing image acquisition, applying additional dose, or introducing more optics. Our method (i) introduces synthetic aberrations to images acquired on the shallow side of image stacks, making them resemble those acquired deeper into the volume and (ii) trains neural networks to reverse the effect of these aberrations.

Antibiotic Length of Therapy: Is Shorter Better in Older Adults?

Antibiotic lengths of therapy (LOT) vary widely, based on infection type, antibiotic regimen, and patient characteristics. Longer LOT are associated with increased risk of antibiotic resistance, adverse effects, and health care costs. There are increasing data supporting shorter LOT for many infections based on randomized, controlled trials (RCTs).

An evaluation framework for clinical use of large language models in patient interaction tasks.

The integration of large language models (LLMs) into clinical diagnostics has the potential to transform doctor-patient interactions. However, the readiness of these models for real-world clinical application remains inadequately tested. This paper introduces the Conversational Reasoning Assessment Framework for Testing in Medicine (CRAFT-MD) approach for evaluating clinical LLMs.

Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers.

Individual ion mass spectrometry (IMS) is the Orbitrap-based extension of the niche mass spectrometry technique known as charge detection mass spectrometry (CDMS). While traditional CDMS analysis is performed on in-house-built instruments such as the electrostatic linear ion trap, IMS extends CDMS analysis to Orbitrap analyzers, allowing charge detection analysis to be available to the scientific community at large. IMS simultaneously measures the mass-to-charge ratios (m/z) and charges (z) of hundreds to thousands of individual ions within one acquisition event, creating a spectral output directly into the mass domain without the need for further spectral deconvolution.

Dissecting tumor transcriptional heterogeneity from single-cell RNA-seq data by generalized binary covariance decomposition.

Profiling tumors with single-cell RNA sequencing has the potential to identify recurrent patterns of transcription variation related to cancer progression, and to produce therapeutically relevant insights. However, strong intertumor heterogeneity can obscure more subtle patterns that are shared across tumors. Here we introduce a statistical method, generalized binary covariance decomposition (GBCD), to address this problem.

Repairing a deleterious domestication variant in a floral regulator gene of tomato by base editing.

Crop genomes accumulate deleterious mutations-a phenomenon known as the cost of domestication. Precision genome editing has been proposed to eliminate such potentially harmful mutations; however, experimental demonstration is lacking. Here we identified a deleterious mutation in the tomato transcription factor SUPPRESSOR OF SP2 (SSP2), which became prevalent in the domesticated germplasm and diminished DNA binding to genome-wide targets.

The impact of common and rare genetic variants on bradyarrhythmia development.

To broaden our understanding of bradyarrhythmias and conduction disease, we performed common variant genome-wide association analyses in up to 1.3 million individuals and rare variant burden testing in 460,000 individuals for sinus node dysfunction (SND), distal conduction disease (DCD) and pacemaker (PM) implantation. We identified 13, 31 and 21 common variant loci for SND, DCD and PM, respectively.

Mechanical regulation of macrophage metabolism by allograft inflammatory factor 1 leads to adverse remodeling after cardiac injury.

Myocardial infarction (MI) mobilizes macrophages, the central protagonists of tissue repair in the infarcted heart. Although necessary for repair, macrophages also contribute to adverse remodeling and progression to heart failure. In this context, specific targeting of inflammatory macrophage activation may attenuate maladaptive responses and enhance cardiac repair.

Cell-free biodegradable electroactive scaffold for urinary bladder tissue regeneration.

Tissue engineering heavily relies on cell-seeded scaffolds to support the complex biological and mechanical requirements of a target organ. However, in addition to safety and efficacy, translation of tissue engineering technology will depend on manufacturability, affordability, and ease of adoption. Therefore, there is a need to develop scalable biomaterial scaffolds with sufficient bioactivity to eliminate the need for exogenous cell seeding.

A soft thermal sensor for the continuous assessment of flow in vascular access.

Hemodialysis for chronic kidney disease (CKD) relies on vascular access (VA) devices, such as arteriovenous fistulas (AVF), grafts (AVG), or catheters, to maintain blood flow. Nonetheless, unpredictable progressive vascular stenosis due to neointimal formation or complete occlusion from acute thrombosis remains the primary cause of mature VA failure. Despite emergent surgical intervention efforts, the lack of a reliable early detection tool significantly reduces patient outcomes and survival rates.

Prevalence of indications of alcohol and drug use among patients treated for injurious falls by Emergency Medical Services in the USA.

Objective: The association between alcohol consumption and increased injuries from falls is well established, but there is a lack of data on the prevalence of substance use by fall type. This study aims to describe the distribution of alcohol and drug involvement in injurious falls.

Methods: Using the 2019 National Emergency Medical Services (EMS) Information System data set, we identified 1 854 909 patients injured from falls requiring an EMS response and determined the fall location (eg, indoors or on street/sidewalk).

Childhood predictors of high school sport participation and effects of participation on young adult activity and mental health.

Introduction: In the United States, sport is a common form of youth physical activity (PA) with demonstrated health benefits. However, limited longitudinal dataexists on the psychosocial determinants and consequences of youth sport participation. This study examined grade 6 (11-12-year-old) predictors of high school organized sport participation and effects of high school sport participation on age 26 behavior, mental health and wellbeing.

Bacillus subtilis-derived-exopolysaccharide halts depigmentation and autoimmunity in vitiligo.

Vitiligo has a complex multifactorial etiology involving a T-cell mediated autoimmune response to cutaneous melanocytes. Microbial dysbiosis has been assigned a contributing role in vitiligo etiology. Treating vitiligo can be a challenging task and finding novel treatment approaches is crucial.

Biological Sex Differences in Rhinitis Prevalence Among Adults in the United States: An "All of Us" Research Program Database Analysis.

Female sex is associated with allergic rhinitis among adults, but not with nonallergic rhinitis. These findings could support a role of sex hormones in the pathogenesis of allergic rhinitis and nasal inflammation, but more research is needed to establish causation.

Cigarette smoking and symptom burden: baseline results from 9 ECOG-ACRIN cancer clinical trials.

Context: Approximately 11% of cancer survivors smoke post-diagnosis.

Objective: Understanding the relationship between smoking and perceived cancer-related symptoms may inform tobacco treatment interventions for this population.

Methods: From 2017-2021, 740 adults in 9 ECOG-ACRIN trials provided baseline data.

Working Conditions in Complementary and Integrative Healthcare Professions.

Objective: The study aimed to describe working conditions among complementary and integrative healthcare (CIH) providers, specifically acupuncturists, chiropractors, massage therapists, midwives, and naturopathic doctors.

Methods: We used cross-sectional Occupational Information Network data (2013-2021) for five CIH occupations. We examined means and 95% confidence intervals (CIs) for 10 variables that represented positive conditions, ergonomic demands, psychosocial demands, and schedule demands.

Performing a Multicenter Retrospective Study.

Multicenter retrospective studies can provide a pragmatic approach to evaluating uncommon pediatric conditions and are less expensive than prospective research. A well-executed retrospective multicenter study, with rigorous study design, systematic data collection, and robust statistical analysis, can produce clinically important and generalizable findings A variety of observational designs can be employed, including cross-sectional, cohort, and case-control studies. Selection bias, ascertainment bias, and confounding are common issues in retrospective research.

Influencing factors: Unveiling patterns and reasons in telehealth care utilization and adoption/avoidance decisions.

Background: The rapid expansion of telehealth, accelerated by the COVID-19 pandemic, has highlighted gaps in understanding demographic and health factors shaping its use. Exploring reasons behind individuals' choices regarding telehealth can guide strategies to promote adoption among diverse populations.

Methods: Data from 5,119 participants in the 2022 Health Information National Trends Survey were analyzed.

Linking Black women to PrEP care using warm handoff referrals from emergency departments to local PrEP clinics.

New Human Immunodeficiency Virus (HIV) cases continue to disproportionately burden cisgender Black/African American women in the United States due to a confluence of structural and systemic factors. Pre-exposure prophylaxis (PrEP) is a safe and effective HIV prevention option, yet there is a striking gap between PrEP eligibility and uptake among cisgender Black women. The current study evaluates a novel warm handoff process in a hospital emergency department setting linking eligible women to local PrEP clinics within 72 hours of hospital discharge in a large southwestern metropolitan city.

Nomadic Nanomedicines: Medicines Enabled by the Paracrine Transfer Effect.

In nanomedicine, the cellular export of nanomaterials has been less explored than uptake. Traditionally viewed in a negative light, recent findings highlight the potential of nanomedicine export to enhance therapeutic effects. This Perspective examines key pathways for export and how nanomaterial design affects removal rates.