Cell cryopreservation typically uses highly concentrated cryoprotectants (CPAs) that can be toxic and cause osmotic stress.
A new method called forced-convective vitrification with liquid oxygen (LOX(bbp)) improves cooling rates and reduces CPA concentration needed for effective vitrification.
Test results show that this new approach significantly increases cooling rates and lowers the necessary CPA concentration, leading to less toxicity and osmotic stress during the vitrification process.