Clinical significance of UGT1A1 polymorphism and expression of ERCC1, BRCA1, TYMS, RRM1, TUBB3, STMN1 and TOP2A in gastric cancer.

Background: Individualized therapeutic regimen is a recently intensively pursued approach for targeting diseases, in which the search for biomarkers was considered the first and most important. Thus, the goal of this study was to investigate whether the UGT1A1, ERCC1, BRCA1, TYMS, RRM1, TUBB3, STMN1 and TOP2A genes are underlying biomarkers for gastric cancer, which, to our knowledge, has not been performed.

Methods: Ninety-eight tissue specimens were collected from gastric cancer patients between May 2012 and March 2015.

Gastric-jejunum pouch side-to-end anastomosis: a novel and safe operation of gastrojejunostomy for preventing reflux gastritis.

Purpose: This study aims to introduce a simple operation method of gastrojejunostomy for preventing reflux esophagitis --gastric-jejunum pouch side-to-end anastomosis.

Methods: Based on Billroth II anastomosis (Billroth II) method, we designed a new technique of anastomosis between stomach wall and jejunal pouch. The technique was named gastric-jejunum pouch side-end anastomosis.

Retrocrural space involvement on computed tomography as a predictor of mortality and disease severity in acute pancreatitis.

Background: Because computed tomography (CT) has advantages for visualizing the manifestation of necrosis and local complications, a series of scoring systems based on CT manifestations have been developed for assessing the clinical outcomes of acute pancreatitis (AP), including the CT severity index (CTSI), modified CTSI, etc. Despite the internationally accepted CTSI having been successfully used to predict the overall mortality and disease severity of AP, recent literature has revealed the limitations of the CTSI. Using the Delphi method, we establish a new scoring system based on retrocrural space involvement (RCSI), and compared its effectiveness at evaluating the mortality and severity of AP with that of the CTSI.

Anatomic pathways of peripancreatic fluid draining to mediastinum in recurrent acute pancreatitis: visible human project and CT study.

Background: In past reports, researchers have seldom attached importance to achievements in transforming digital anatomy to radiological diagnosis. However, investigators have been able to illustrate communication relationships in the retroperitoneal space by drawing potential routes in computerized tomography (CT) images or a virtual anatomical atlas. We established a new imaging anatomy research method for comparisons of the communication relationships of the retroperitoneal space in combination with the Visible Human Project and CT images.

SLC gene-modified dendritic cells mediate T cell-dependent anti-gastric cancer immune responses in vitro.

Dendritic cells (DCs) are potent professional antigen-presenting cells (APCs) with the ability to prime naïve T cells, and play an important role in the initiation and regulation of immune responses. In this study, we constructed a recombinant adenovirus carrying the SLC gene (Ad-SLC), and detected the biological effects of Ad-SLC-modified DCs as an adjuvant for the initiation of gastric cancer immune responses. Human DCs were transfected with Ad-SLC and the recombinant adenovirus carrying the β-galactosidase gene, Ad-LacZ, respectively.

Association between IL-4 polymorphism and acute rejection of solid organ allograft: a meta-analysis.

Background: Cytokines have been implicated in the acute rejection of solid organ transplantation. Many studies have investigated the association between recipient or donor IL-4 polymorphism and acute rejection, with different studies reporting inconclusive results.

Methods: We searched PUBMED and EMBASE until June 2012 to identify eligible studies investigating the association between IL-4 polymorphism with acute rejection after solid organ transplantation.

Detection of bacterial diversity in rat's periodontitis model under imitational altitude hypoxia environment.

Objectives: Oral epidemiologic investigations in China western territory have showed that the immigrants in the plateau have a higher morbidity with periodontitis. To find the possible relationship between the pathogenesis of periodontitis and altitude hypoxia, we designed a periodontitis rat model via housed in low pressure oxygen chamber and investigated the bacterial diversity in the gingival crevicular fluid (GCF).

Design: Eighty Sprague-Dawley rats were divided into CON-normal, CON-hypoxia, EXP-normal and EXP-hypoxia group, without or with periodontal induce, breeding in normal environment or altitude hypoxia environment.

Hemoglobin-based oxygen carriers combined with anticancer drugs may enhance sensitivity of radiotherapy and chemotherapy to solid tumors.

The aberrant vascular architecture in solid tumors is the key limiting factor known to ameliorate hypoxia and increase circulating anticancer drugs, thus resulting in resistance to radiotherapy and chemotherapy in tumor treatment. Previous experiments have reported hemoglobin-based oxygen carriers (HBOCs) that are effective to improve tumor oxygenation, thereby serving as potential agents target-oriented to the hypoxic tumor. Herein, we draw the hypothesis that HBOCs combined with an anticancer drug may increase oxygen bioavailability and anticancer drug retention in solid tumors and in turn contribute to enhanced sensitivity of radiotherapy and chemotherapy.

[Influence of different spacer thickness and spacer coverage area on the adaptation of Cercon CAD/CAM copings].

Objective: To evaluate the accuracy of Cercon CAD/CAM system and the influence of different spacer thickness and spacer coverage area on the internal and marginal fit of the copings.

Methods: A stainless steel master model of first molar was replicated. After scanning, designs of copings were made with different spacer thickness and spacer coverage area.

[Effects of predator stress on the expression of cAMP responsive element binding protein in hippocampus: experiment with rats].

Objective: To explore the neurobiological basis involved in the pathogenesis of the lasting emotionality and cognitive impairment following severe psychological stress.

Methods: Ninety-six male Wistar rats were divided randomly into 2 equal groups: group of predator stress (Group PS) put into a cage in the experimental box singly to be exposed to a cat in the box but outside the cage for 23-57 min until tremor, polypnea, and nares flaring appeared for 6 min so as to establish predator stress models, and control group, put into the cage without non-injurious exposure of cat. 1, 12, and 24 hours later 8 rats from each group were killed with the hippocampus taken out.

[Soluble expression, purification of bone morphogenetic protein-2 fragment and its antibody preparation].

The bone morphogenetic protein-2 (BMP-2) fragment (BMP-2omega, 606-846bp) cDNA was amplified from total RNA of SAOS-2 cells by using RT-PCR. The PCR product was then inserted into pET-28a (+) vector for constructing the expression plasmid that would be used to transform the host cell BL21(DE3). After IPTG inducing under different conditions, this BMP-2w protein could be expressed in high level as a soluble form, and purified by chelating column (Ni-NTA).

[Effect of hepatocyte growth factor on intestinal permeability and bacterial translocation after small bowel transplantation in rat].

Objective: To evaluate the effect of hepatocyte growth factor (HGF) on intestinal permeability and bacterial translocation after small bowel transplantation in rats.

Methods: Twenty Wistar rats were as receptors and twenty SD rats as donors. After heterotopic intestinal grafting, cyclosporine A was administered at 6 mg/kg x day intramuscularly for inhibiting rejection.

Dendritic cells modified with 6Ckine/IFNgamma fusion gene induce specific cytotoxic T lymphocytes in vitro.

Background And Objective: [corrected] Dendritic cells play an important role in initiation and regulation of immune responses. Previous studies demonstrated that intratumoral administration of 6Ckine-modified DCs enhanced local and systemic antitumor effects. Herein we report the investigation of the specific CTL responses elicited by adenoviral 6Ckine/IFNgamma fusion gene-modified DCs in vitro.

[Construction of recombinant adenovirus carrying human IFNgamma gene by in vitro ligation and its expression].

Background & Objective: Interferon-gamma (IFNgamma) can promote directly the apoptosis of some kinds of tumor cells and regulate cellular immunity, therefore, it may play an important role in gene therapy for tumors. This study was to construct recombinant adenovirus carrying human IFNgamma cDNA, and detect its expression profile in eukaryotic cells.

Methods: IFNgamma cDNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) from human peripheral blood mononuclear cells, and cloned into adenoviral shuttle plasmid pShuttle to construct recombinant adenovirus carrying human IFNgamma cDNA (Ad-IFNgamma) by in vitro ligation.

[The clinical effect of anti-rotation reduction internal fixator on the treatment of fresh thoracolumbar spine fracture].

Objective: To evaluate the effect of self-designed anti-rotation reduction internal fixator (ARRIF) on treating different spine segment fracture.

Methods: From August 1999 to March 2003, 76 patients(48 males and 28 females, aged from 22 to 59 with an average of 34.1) with thoracolumbar fracture were operatively treated by ARRIF.

[Effect of dehydroepiandrosterone on Ca(2+)-activated K+ channel of pulmonary arterial smooth muscle cells in pulmonary hypertensive rats].

Objective: To investigate the effects of dehydroepiandrosterone (DHEA) on Ca(2+)-activated K(+) (K(Ca)) channel and mean pulmonary arterial pressure (mPAP) in rats with chronic pulmonary hypertension.

Methods: Fifty Wistar rats were divided randomly into a normal group (group A, n = 10) and a chronic hypoxia group (group B, n = 40). The rats in group B were subdivided into group B(1), B(2), B(3), and B(4) (each n = 10) at random.

[Clinical significance of trigeminal neuralgia treated using radiofrequency thermocoagulation (RFT) with different approaches].

Objective: The authors presented the short term and long term effects of the trigeminal neuralgia treated by RFT with anterior-lateral facial, lateral facial and trans-subzygomatic approaches.

Methods: 320 primary patients with trigeminal neuralgia were selected for this study. All these patients were followed up with the period of 1 to 6 years.

[Endoscopic reconstruction of 192 patients with traumatic urethral stenosis].

We treated traumatic urethral stenosis with endoscopic technique and observed effects. 192 men with traumatic urethral stenosis (47 with anterior urethral stenosis and 145 with posterior urethral stenosis) were treated with hydroelectric shock wave, cold knife, microwave and electroincission through transurethral endoscopy. We punched hole in the centre of the scar with hydroelectric shock waves for long segment urethral complete occlution.

[Is anomalous junction of pancreaticobiliary duct related to pancreatitis].

Among 1779 patients was subjected to endoscopic retrograde cholangiopancreatography (ERCP), an anomalous junction of pancreaticobiliary duct (AJPB) was found in 48 (AJPB group), a normal common channel was found in 734 (control group A), and a non-common channel was found in 284 (control group B). Another 713 patients were excluded because the pancreaticobiliary duct was not clear. In AJPB group, the length of the common channel ranged from 15 to 35mm (17.

A new method to localize brain nuclei for surgery in extrapyramidal disease.

A CT localization method to be used with the Leksell stereotactic system was employed to locate the ventral lateral nucleus of the thalamus (VL) and globus pallidus (GP) for treating extrapyramidal disease (EPD). The study comprised 100 normal adults and 70 cases of EPD, and results in both populations were compared. The 70 cases of EPD were studied ventriculography, and 30 normal adults were studied my MRI.

[Clinical application of the single radial immunodiffusion (SRID) technic to detect antitubercle bacillus antibody].

Using the Tubercle bacillus Danish D1331 atoxic species as antigen and by single radial immunodiffusion (SRID) technic, the serum anti-TB-Ab of 454 patients with tuberculosis or other diseases were assayed and the results were reported. It was demonstrated that the sensitivity, specificity and accuracy of this method to diagnose tuberculosis were 81.78%, 97.

[Surgical treatment of multiple accessory pathways in Wolff-Parkinson-White syndrome].

The electrophysiological sign of multiple accessory pathways in Wolff-Parkinson-White syndrome (WPW syndrome) is complicated. Surgical interruption is difficult in those patients. In 8 patients with multiple accessory pathways in the WPW syndrome, 5 were treated by local intramyocardial injection of absolute alcohol, and 3 by dissection of accessory pathways under cardiopulmonary bypass.