5 results match your criteria: "Chemistry and Pharmacy University of Southern Denmark[Affiliation]"
Design and in vitro anticancer assessment of a click chemistry-derived dinuclear copper artificial metallo-nuclease.
Nucleic Acids Res
January 2025
School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
Copper compounds with artificial metallo-nuclease (AMN) activity are mechanistically unique compared to established metallodrugs. Here, we describe the development of a new dinuclear copper AMN, Cu2-BPL-C6 (BPL-C6 = bis-1,10-phenanthroline-carbon-6), prepared using click chemistry that demonstrates site-specific DNA recognition with low micromolar cleavage activity. The BPL-C6 ligand was designed to force two redox-active copper centres-central for enhancing AMN activity-to bind DNA, via two phenanthroline ligands separated by an aliphatic linker.
View Article and Find Full Text PDFNatamycin interferes with ergosterol-dependent lipid phases in model membranes.
BBA Adv
August 2023
Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230, Odense M, Denmark.
Natamycin is an antifungal polyene macrolide that is used as a food preservative but also to treat fungal keratitis and other yeast infections. In contrast to other polyene antimycotics, natamycin does not form ion pores in the plasma membrane, but its mode of action is poorly understood. Using nuclear magnetic resonance (NMR) spectroscopy of deuterated sterols, we find that natamycin slows the mobility of ergosterol and cholesterol in liquid-ordered (Lo) membranes to a similar extent.
View Article and Find Full Text PDFMitochondrial reactive oxygen species modify extracellular vesicles secretion rate.
FASEB Bioadv
September 2023
Department of Molecular Medicine, Cardiovascular and Renal Research University of Southern Denmark Odense Denmark.
Article Synopsis
- Hypoxia boosts the secretion rate of extracellular vesicles (EVs) by increasing reactive oxygen species (ROS) through the mitochondrial electron transport chain (ETC) and hypoxia-induced factor (HIF)-1 signaling, but their exact roles in this process were previously unclear.* -
- The study found that while the HIF-1α stabilizer roxadustat did not affect EV secretion, stimulation of the ETC with dichloroacetic acid (DCA) significantly increased EV release, but this was blocked by the antioxidant TEMPO and the Complex I inhibitor rotenone.* -
- Inhibition of Complex III under hypoxic conditions and early steps in the mevalonate pathway enhanced ROS-dependent EV secretion,
Editorial: Enzyme or Whole Cell Immobilization for Efficient Biocatalysis: Focusing on Novel Supporting Platforms and Immobilization Techniques.
Front Bioeng Biotechnol
March 2021
Department of Physics, Chemistry and Pharmacy University of Southern Denmark, Odense, Denmark.
Facile Synthesis of Enzyme-Embedded Metal-Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent.
Front Bioeng Biotechnol
July 2020
Department of Physics, Chemistry and Pharmacy University of Southern Denmark, Odense, Denmark.
immobilization of enzyme into metal-organic frameworks (MOFs) is performed through a one-step and facile method. lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO)⋅6HO] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent.
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