LINC00673 binds with leptin to regulate osteogenic function in periodontal ligament stem cells.

Background: The aim of this study is to investigate whether LINC00673 and leptin are associated with osteogenesis of periodontal ligament stem cells in the microenvironment of advanced glycation end products.

Methods: LINC00673 expression was detected in PDLSCs by qRT-PCR performed during osteogenic differentiation. By using alkaline phosphatase and Alizarin red S staining, we were able to confirm the role of LINC00673 in regulating osteogenesis in PDLSCs.

A novel 5'tRNA-derived fragment tRF-Tyr inhibits tumor progression by targeting hnRNPD in gastric cancer.

Background: Transfer RNA-derived small RNAs (tsRNAs), including tRNA-derived fragments (tRFs) and tRNA halves (tiRNAs), constitute a novel class of small noncoding RNAs (sncRNAs). tsRNAs have been linked to tumorigenesis and the progression of carcinogenesis; however, the precise molecular mechanism through which tRFs act in gastric cancer (GC) remains unknown.

Methods: tRF-Tyr is a potential GC tumor suppressor that was identified through high-throughput sequencing technology.

The TRPM7 chanzyme in smooth muscle cells drives abdominal aortic aneurysm in mice.

Ionic signaling in smooth muscle cells (SMCs) is critical for vascular homeostasis. In this study, we untangled the role of the bifunctional TRPM7 channel kinase (chanzyme) in abdominal aortic aneurysm (AAA) pathogenesis. Comparing SMC-specific, macrophage-specific and endothelial cell-specific Trpm7 knockout, we revealed that SMC-specific Trpm7 deficiency protected mice from AAA in two distinct preclinical models of the disease.

Prospects of black phosphorus nanosheets in the treatment of peri-implantitis.

Peri-implantitis represents an inflammatory condition characterized by the presence of plaque-related soft and hard tissue damage surrounding dental implants, often resulting in progressive alveolar bone loss and, ultimately, implant failure. Black phosphorus (BP), a novel two-dimensional (2D) material that has recently emerged in the biomedical field, has attracted increasing attention due to its unique osteogenic properties and exceptional antibacterial and antioxidant characteristics. Additionally, its outstanding biomedical attributes enhance angiogenesis and nerve regeneration.

TiltRec: an ultra-fast and open-source toolkit for cryo-electron tomographic reconstruction.

Motivation: Cryo-electron tomography (cryo-ET) has revolutionized our ability to observe structures from the subcellular to the atomic level in their native states. Achieving high-resolution reconstruction involves collecting tilt series at different angles and subsequently backprojecting them into 3D space or iteratively reconstructing them to build a 3D volume of the specimen. However, the intricate computational demands of tomographic reconstruction pose significant challenges, requiring extensive calculation times that hinder efficiency, especially with large and complex datasets.

The Role of Lymphocyte Recovery Index in Prognosis Prediction for Locally Advanced Cervical Cancer With Radiation-Induced Lymphopenia.

Background: In patients with locally advanced cervical cancer (LACC) undergoing concurrent chemoradiotherapy (CCRT), the high incidence of radiation-induced lymphopenia significantly affects prognosis. There are significant variations in lymphocyte count (ALC) recovery patterns among patients, and their impact on prognosis remains unclear. This study aims to quantify the lymphocyte recovery patterns by the lymphocyte recovery index (LRI) and evaluate its prognostic value.

Combination of Evans syndrome and COVID-19: a systematic review of reported cases.

Background: Evans syndrome is a rare autoimmune disease characterized by simultaneous or sequential primary immune thrombocytopenia and autoimmune hemolytic anemia. Despite the low incidence of Evans syndrome after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, its progression may threaten public health. This review offers an up-to-date summary of the works on the association between coronavirus disease 2019 (COVID-19) and Evans syndrome to explore the pathogenic mechanisms, epidemiological characteristics, clinical presentations, diagnostic markers, and treatment strategies.

TNFAIP2 promotes NF-κB signaling mediate lymph node metastasis of oral squamous cell carcinoma by protecting IKKβ from ubiquitin proteasome degradation.

Background: Tumor dissemination is a life-threatening event which confers to most cancer-related deaths with limited effective therapeutic option. TNFα-induced protein 2 (TNFAIP2) reveals pro-metastasis potential in several cancers. However, its definite role and underlying mechanism in oral squamous cell carcinoma (OSCC) is largely unknown.

Advances in the development of phosphodiesterase 5 inhibitors.

Phosphodiesterase 5 (PDE5) can hydrolyze cyclic guanosine monophosphate (cGMP), which is critical for maintaining various physiological processes in organisms. Currently, clinically approved indications for PDE5 inhibitors encompass therapeutic agents for erectile dysfunction (ED), symptoms associated with lower urinary tract symptoms (LUTS), and pulmonary artery hypertension (PAH). Despite the fact that the development of selective PDE5 inhibitors has been a significant focus in drug development for some time following the proven success of sildenafil as a PDE5 inhibitor for ED treatment, fewer than ten drugs in this therapeutic class have been marketed in the past 25 years, often accompanied by adverse effects.

Association of killer cell immunoglobulin-like receptor genes with primary Sjögren's syndrome among Chinese population.

Background: Primary Sjögren's syndrome (pSS) is a chronic and common autoimmune disorder which is caused by inflammatory infiltration of the exocrine glands salivary glands and lacrimal glands are the main sites of involvement. Killer cell immunoglobulin-like receptors (KIRs), are crucial for the development and function of natural killer (NK) cells. Moreover, the activity of NK cells is affected by the interaction of KIRs with HLA class I ligands.

Ultraprecise Detection of Influenza Virus by Antibody-Modified Graphene Transistors.

Over the past decade, the large-scale spread of influenza viruses has posed an increasing burden on public health. The effective screening of influenza agents requires a fast, precise, on-site and easy-to-operate method. Unfortunately, current screening methods face challenges in speed and accuracy, especially in complex on-site settings.

Correction: Roy et al. Metformin and ICG-001 Act Synergistically to Abrogate Cancer Stem Cells-Mediated Chemoresistance in Colorectal Cancer by Promoting Apoptosis and Autophagy. 2022, , 1281.

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Design, Synthesis, and Bioactivity Evaluation of Novel 1-Methyl-2-phenylpyridin-1-ium Derivatives as Broad-Spectrum FtsZ Inhibitors.

To address the threat of bacterial infections in animal husbandry, a novel class of 1-methyl-2-phenylpyridin-1-ium derivatives has been designed and synthesized as broad-spectrum antibacterial agents to counteract increasing multidrug resistance. Biological assays revealed that compounds , , , and exhibited superior inhibition of ATCC25923 (MICs = 0.0625-0.

Transcriptomics-Driven Discovery of New Meroterpenoid Rhynchospenes Involved in the Virulence of the Barley Pathogen .

, the causal agent of barley scald disease, poses a major threat to global barley production. Despite its significant impact, the molecular mechanisms underlying 's infection process remain largely unexplored. To address this, we analyzed the differential gene expression data of WAI453 cultivated under both and conditions, aiming to identify secondary metabolite biosynthetic gene clusters that are potentially involved in the pathogenicity of .

Recent advances in nutritional metabolism studies on SARS-CoV-2 infection.

In the context of the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), metabolic research has become crucial for in-depth exploration of viral infection mechanisms and in searching for therapeutic strategies. This paper summarizes the interrelationships between carbohydrate, lipid, and amino acid metabolism and COVID-19 infection, discussing their roles in infection progression. SARS-CoV-2 infection leads to insulin resistance and increased glycolysis, reducing glucose utilization and shifting metabolism to use fat as an energy source.

Compound identification of Shuangxinfang and its potential mechanisms in the treatment of myocardial infarction with depression: insights from LC-MS/MS and bioinformatic prediction.

Background: Patients with myocardial infarction (MI) have a high incidence of depression, which deteriorates the cardiac function and increases the risk of cardiovascular events. Shuangxinfang (Psycho-cardiology Formula, PCF) was proved to possess antidepressant and cardioprotective effects post MI. However, the compounds of PCF remain unidentified, and the pertinent mechanism is still not systematic.

Mapping knowledge landscapes and emerging trends in artificial intelligence for antimicrobial resistance: bibliometric and visualization analysis.

Objective: To systematically map the knowledge landscape and development trends in artificial intelligence (AI) applications for antimicrobial resistance (AMR) research through bibliometric analysis, providing evidence-based insights to guide future research directions and inform strategic decision-making in this dynamic field.

Methods: A comprehensive bibliometric analysis was performed using the Web of Science Core Collection database for publications from 2014 to 2024. The analysis integrated multiple bibliometric approaches: VOSviewer for visualization of collaboration networks and research clusters, CiteSpace for temporal evolution analysis, and quantitative analysis of publication metrics.

Preliminary outcomes of Neuroform Atlas stent-assisted coiling for intracranial aneurysms with small parent vessels.

Background: Although stent-assisted coiling has become a standard approach for treating intracranial aneurysms (IAs), there are limited reports on its safety and effectiveness in parent artery less than 2.5 mm in diameter. This study evaluates the feasibility, safety, and short-term outcomes of using Neuroform Atlas stent-assisted coiling for IAs with small parent vessels.

Effects of 0.01 mM strontium on human periodontal ligament stem cell osteogenic differentiation via the Wnt/-catenin signaling pathway.

Objectives: Strontium (Sr) is a crucial trace element in humans, mainly present in the bones. We investigated the effects of Sr on human periodontal ligament stem cell (hPDLSC) proliferation and osteogenesis and the relevant pathways.

Methods: hPDLSCs were harvested from extracted premolars and characterized by flow cytometry, then cultured and treated with various Sr concentrations.

Coordinated excitatory and inhibitory circuits governing unpredictable threat-induced anxiety.

Anxiety is elicited by excessive apprehension about unpredictable threats. However, the neural circuit governing unpredictable threat induced anxiety remains unclear. Here, we found ventral bed nucleus of the stria terminalis (vBNST) GABAergic neurons displayed selective activation to unpredictable threats through coordinated excitatory input from insular cortex glutamergic neurons and inhibitory input from the lateral nucleus of the amygdala (CeL) somatostatin neurons.

Advancements in PROTAC-based therapies for neurodegenerative diseases.

Neurodegenerative diseases are characterized by impairments in movement and cognitive functions. These disorders are frequently associated with the accumulation of misfolded protein aggregates, which present significant challenges for treatment with conventional small-molecule inhibitors. While FDA-approved amyloid-beta-directed antibodies, such as Lecanemab, have recently shown clinical success in modifying disease progression, there are currently no treatments capable of curing neurodegenerative diseases.

Synthesis, biological and pharmacokinetic characterization of a novel leucine ureido derivative as a multi-target anticancer agent.

Previously, a novel series of leucine ureido derivatives containing the 1,2,3-triazole moiety were identified and validated as potent aminopeptidase N inhibitors with marked and antitumor potencies. Moreover, synergistic anti-proliferation effects against tumor cells were found when used in combination with 5-Fluorouracil (5-FU). Herein, a novel leucine ureido derivative (compound 3) was synthesized by coupling cytotoxic agent 5-FU with leucine ureido derivatives containing the 1,2,3-triazole moiety esterification.