Multiple patterns of EEG parameters and their role in the prediction of patients with prolonged disorders of consciousness.

Introduction: Prognostication in patients with prolonged disorders of consciousness (pDoC) remains a challenging task. Electroencephalography (EEG) is a neurophysiological method that provides objective information for evaluating overall brain function. In this study, we aim to investigate the multiple features of pDoC using EEG and evaluate the prognostic values of these indicators.

Polymorphisms in immunosuppression-related genes are associated with AML.

Background: Acute myeloid leukemia (AML) is a hematologic malignancy with poor overall survival (OS). The immunosuppressive microenvironment significantly impacts AML development and chemoresistance. Despite new immunotherapeutic strategies entering standard clinical care for various tumors, progress in AML remains poor.

The lncRNA MIR181A1HG in extracellular vesicles derived from highly metastatic colorectal cancer cells promotes liver metastasis by remodeling the extracellular matrix and recruiting myeloid-derived suppressor cells.

Background: Colorectal liver metastasis (CRLM) is the main cause of death in colorectal cancer (CRC) patients worldwide. In the initial stage of metastasis, primary tumors provide the necessary conditions for metastasis by shaping the local microenvironment of the target organ, forming "premetastatic niches" (PMNs), and extracellular vesicles (EVs) play important roles in shaping PMNs. Therefore, investigating the EVs involved in the regulation of PMNs and their mechanism is highly valuable for the further understanding of CRLM.

Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP.

Periodontal ligament stem cell (PDLSC)-based tissue engineering is an important method to promote periodontal tissue regeneration. However, PDLSCs are susceptible to the effects of replicative senescence, leading to reduced proliferation and differentiation abilities and weakened tissue regeneration potential. Senolytics (the combination of dasatinib and quercetin) are drugs that inhibit cellular aging through inducing the apoptosis of senescent cells, but whether they have positive effects during the senescence of PDLSCs is unknown.

Rejuvenation Modulation of Nucleus Pulposus Progenitor Cells Reverses Senescence-Associated Intervertebral Disc Degeneration.

The decreased regeneration potential of aging nucleus pulposus resident progenitor cells (NPPCs) fails to resist intervertebral disc degeneration (IVDD), and strategies to remodel the regeneration capacity of senescent NPPC are urgently needed. A decrease in Klotho gene expression in NPPCs of both old mice and humans exacerbates the impaired regenerative functionality of NPPC. Here, an NPPC-targeted lipid thymine nanoparticle (NT-LNP) is reported for the in situ manipulation of the regenerative repair potential of NPPCs, restoration of degenerated nucleus pulposus tissue, and mitigation of IVDD.

Cellular Spatial Sensing Determines Cell Mechanotransduction Activity on the Aligned Nanofibers.

The geometric properties of extracellular matrix (ECM) fibers play a crucial role in regulating cellular behaviors and functions. Although extensive research has examined the effects of fiber alignment, conflicting results have often arisen, leaving the precise mechanisms by which electrospun fiber alignment affects cellular behavior still unclear. This study investigates how the arrangement of polycaprolactone (PCL) electrospun fiber substrates affects cellular mechanosensing by modulating cell positioning.

Atypical audio-visual neural synchrony and speech processing in early autism.

Background: Children with Autism Spectrum disorder (ASD) often exhibit communication difficulties that may stem from basic auditory temporal integration impairment but also be aggravated by an audio-visual integration deficit, resulting in a lack of interest in face-to-face communication. This study addresses whether speech processing anomalies in young autistic children (mean age 3.09-year-old) are associated with alterations of audio-visual temporal integration.

A force-sensitive adhesion GPCR is required for equilibrioception.

Equilibrioception (sensing of balance) is essential for mammals to perceive and navigate the three-dimensional world. A rapid mechanoelectrical transduction (MET) response in vestibular hair cells is crucial for detecting position and motion. Here, we identify the G protein-coupled receptor (GPCR) LPHN2/ADGRL2, expressed on the apical membrane of utricular hair cells, as essential for maintaining normal balance.

Population-level analyses identify host and environmental variables influencing the vaginal microbiome.

The vaginal microbiome is critical for the reproductive health of women, yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown. Here, we conducted a comprehensive cross-sectional study of the relationships between the vaginal microbiome and 81 matched host and environmental variables across 6755 Chinese women. By 16S rRNA sequencing, we identified four core vaginal microbiota with a prevalence of over 90% and a total median abundance of 98.

Enhancing diabetic retinopathy diagnosis: automatic segmentation of hyperreflective foci in OCT via deep learning.

Objective: Hyperreflective foci (HRF) are small, punctate lesions ranging from 20 to 50 m and exhibiting high reflective intensity in optical coherence tomography (OCT) images of patients with diabetic retinopathy (DR). The purpose of the model proposed in this paper is to precisely identify and segment the HRF in OCT images of patients with DR. This method is essential for assisting ophthalmologists in the early diagnosis and assessing the effectiveness of treatment and prognosis.

[Analysis of characteristics of anonymous online dating and related factors of not being tested for HIV among men who have sex with men in Shandong Province].

To analyze the characteristics of anonymous online dating and related factors of not being tested for HIV among men who have sex with men and to provide the basis for developing targeted HIV prevention strategies and intervention measures. Using a cross-sectional study design, respondents were recruited with the assistance of MSM social organizations from April to July 2023 in eight cities in Shandong Province. Snowball sampling, activity venues, and networks were employed, targeting a sample size of 400 people per city.

Evaluating the Methodological Rigor and Recommendation Excellence of TAVR Guidelines: Insights from AGREE II and AGREE-REX Instruments.

Purpose: Transcatheter aortic valve replacement (TAVR) has emerged as a critical innovation for managing severe aortic stenosis, prompting the development of numerous clinical practice guidelines worldwide. This study systematically evaluates the guideline development methodologies of major international TAVR guidelines using the AGREE II and AGREE-REX instruments, aiming to enhance understanding of current development processes.

Methods: A comprehensive search was conducted in PubMed, Embase, Web of Science, and specialized guideline repositories.

Deconer: An Evaluation Toolkit for Reference-based Deconvolution Methods Using Gene Expression Data.

In recent years, computational methods for quantifying cell type proportions from transcription data have gained significant attention, particularly those reference-based methods which have demonstrated high accuracy. However, there is currently a lack of comprehensive evaluation and guidance for available reference-based deconvolution methods in cell proportion deconvolution analysis. In this study, we introduce Deconvolution Evaluator (Deconer), a comprehensive toolkit for the evaluation of reference-based deconvolution methods.

Association of a novel nutritional index with cognitive impairment in middle-aged and elderly Chinese adults: a cross-sectional analysis from the China Health and Retirement Longitudinal Study.

Purpose: The triglyceride-cholesterol-body weight index (TCBI), a novel and easily computable nutritional index, incorporates serum triglyceride (TG), total cholesterol (TC), and body weight (BW). This study explored the association between TCBI and cognitive impairment in middle-aged and elderly Chinese populations.

Patients And Methods: This cross-sectional study employed data from the China Health and Retirement Longitudinal Study (CHARLS) baseline survey, including 7,145 participants.

Radiomics model building from multiparametric MRI to predict Ki-67 expression in patients with primary central nervous system lymphomas: a multicenter study.

Objectives: To examine the correlation of apparent diffusion coefficient (ADC), diffusion weighted imaging (DWI), and T1 contrast enhanced (T1-CE) with Ki-67 in primary central nervous system lymphomas (PCNSL). And to assess the diagnostic performance of MRI radiomics-based machine-learning algorithms in differentiating the high proliferation and low proliferation groups of PCNSL.

Methods: 83 patients with PCNSL were included in this retrospective study.

Excessive lymph node dissection increases postoperative cardiovascular disease risk in non-small cell lung cancer patients.

Heart disease is the second leading cause of death in lung cancer (LC) patients, with postoperative cardiovascular disease (CVD) commonly linked to lymph node dissection (LND). This study aimed to clarify the optimal number of lymph nodes (LNs) to be dissected by investigating the impact of LND on CVD related death in patients with surgically resected non-small cell lung cancer (NSCLC). We analyzed data from the Surveillance, Epidemiology, and End Results (SEER) database for a total of 154,566 patients with stage IA-IIIA NSCLC that underwent curative surgery from 2000 to 2020, and further validated using clinical data from a single-center cohort.

Lipin1 ameliorates cognitive ability of diabetic encephalopathy via regulating Ca transfer through mitochondria-associated endoplasmic reticulum membranes.

Diabetic encephalopathy (DE) is a common central nervous system complication resulting from diabetes mellitus (DM). While the exact pathogenesis remains unclear, a homeostatic imbalance of mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) within neurons has been shown to be closely associated with the dysfunctional cognitive pathology of this condition. Our previous work has revealed that phosphatidate phosphatase Lipin1 plays a critical role in the cognitive processes of DE via regulating mitochondrial function.

The suggested dosage and course of thiazide diuretics on preventing recurrent urolithiasis: an upstate meta-analysis.

Background: Over the past few decades, many studies assessing the effect of thiazide diuretics showed controversial results. Additionally, recent guidelines fail to address the optimal dosage and treatment duration of thiazide diuretics. Therefore, we conducted a meta-analysis to evaluate the efficacy, optimal dosage, and treatment duration of thiazide diuretics in preventing recurrent urolithiasis.

A Model to Identify Grey-Zone Patients with Chronic Hepatitis B Requiring Antiviral Therapy: A Multicentre, Retrospective Study.

Background: Individuals who do not match any specific stage in the natural history of chronic hepatitis B are classified into the grey-zone, and appropriate management for these patients remains unclear. This study aimed to develop and validate a non-invasive model to identify grey-zone patients requiring antiviral therapy (AVT).

Methods: We retrospectively collected data on 200 grey-zone patients not requiring AVT (according to assessment by non-invasive parameters from 2010 to 2023 in six hospitals) and randomised them into development (n=140) and validation (n=60) cohorts.

Adhesive micro-liquid for efficient removal of bacterial biofilm infection.

Bacteria are common infectious pathogens that can cause invasive and potentially life-threatening infections. Ionic liquids have emerged as a novel class of alternatives to antibiotics, however their inherent hydrophobicity and immiscible in water exhibits poor adhesion to bacteria and diminishes its utilization and bioavailability for infection control. Herein, an adhesive metal phenolic encapsulated ionic liquid choline and geranate (CAGE@MPN) microcapsules is designed to address the aforementioned challenges and remove bacterial biofilm infections.

Harnessing Membrane Coating for Probiotic Therapy in Inflammatory Bowel Disease.

Inflammatory bowel diseases (IBDs) are characterized by dysregulated inflammatory responses, increased reactive oxygen species (ROS), and compromised intestinal barrier function. Current clinical treatments often yield suboptimal responses and significant side effects. Given the great potential of (AKK) bacterial membrane components in IBD treatment and the possible pathogenicity of live bacteria, to essentially address these challenges, we develop a natural membrane-based single-cell coating, incorporating a self-assembled AKK bacterial membrane (AKM) and astaxanthin (AST), forming a protective biological barrier on probiotics.

Relationship between development trajectories of leisure activity and sleep time on incident cognitive impairment: A study based on the Chinese Longitudinal Healthy Longevity Survey (CLHLS).

Background: Leisure activity and sleep time are key factors in cognitive impairment, but the impact of their long-term trajectories on incident cognitive impairment remains unclear.

Objective: To examine the association of leisure activity trajectories, sleep time trajectories and their combined effects with incident cognitive impairment in older adults.

Methods: Data from the Chinese Longitudinal Healthy Longevity Survey (2008-2018) were analyzed, including adults aged ≥65 who participated in at least three surveys.

Determination of arsenic speciation of arsenic in squid and its human consumption risk.

Arsenic species were quantified to further explore the biotransformation of arsenic and better explain the potential health risks. In this research, the levels of six arsenic species (AsB, AsC, MMA, DMA, As, and As) were determined in 160 squid samples by HPLC-ICP-MS. AsB was the most predominant speciation in squid tissues.

Benchtop and different miniaturized near-infrared spectrometers application study: Calibration transfer and 2D-COS for in-situ analysis of moisture content in HPMC.

The demand for miniaturized near-infrared (NIR) spectrometers has surged due to their potential for in-situ analysis. However, their predictive accuracy has not yet matched that of traditional benchtop instruments. This study evaluates the effectiveness of rapid quantitative moisture analysis in hydroxypropyl methylcellulose (HPMC) using a benchtop spectrometer, Antaris II from Thermo Fisher Scientific Inc.