1 results match your criteria: "Charles University and Faculty Hospital Pilsen[Affiliation]"
Sperm antibodies, intra-acrosomal sperm proteins, and cytokines in semen in men from infertile couples.
Am J Reprod Immunol
March 2009
Department of Obstetrics and Gynecology, Charles University and Faculty Hospital Pilsen, Alej svobody 80, Plzen-Lochotin, Czech Republic.
Problem: The aim of this study was to investigate seminal sperm-agglutinating antibodies, intra-acrosomal proteins, sperm head abnormalities, and cytokines (IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70 TNF-alpha, and IFN-gamma) in men from infertile couples.
Method Of Study: The direct mixed anti-immunoglobulin reaction test for IgG, IgA, and IgE in semen, and immunocytochemical method using monoclonal antibodies and indirect immunofluorescence for the examination of intra-acrosomal proteins in the spermatozoa were used. Cytokines in seminal plasma were determined by multiplex immunoanalytic xMAP (LUMINEX) technology.