MicroRNA-92a Regulates Expression of Kruppel-like Factor2 in Rabbit Model of Intracranial Aneurysm.

To explore role of miRNA-92a in regulation of KLF2 expression in intracranial aneurysm model, real time PCR, IHC, ISH and luciferase activity were used to test relationship of miR-92a and KLF2. The results demonstrated that KLF2 showed a time-dependent increase following development of IA model, but the miR-92a showed a time-dependent decrease, co-expression of miR-92a was found to effectively down-regulate luciferase expression in constructs with 3'UTRs of KLF2, to determine the functions of miR-92a in rabbit ECs, we transfected the pre-miR-92a into rabbit ECs for over-expression, the results showed that the protein level of KLF2 was significantly down-regulated when over-expression of miR-92a. In conclusion, our research demonstrated that miR-92a and KLF2 were negative correlation in intracranial aneurysm model, and miR-92a could directly target KLF2 in endothelial cells through complementary sequence of 3'UTR region.

Role of Kruppel-like Factor 2 in Intracranial Aneurysm of the Rabbit Model.

We investigated expression of Kruppel—like factor 2 (KLF2) and its correlation with basilar artery blood flow rate in the hemodynamically induced aneurysm model built by different methods. New Zealand rabbits were randomly divided into sham—operated group, unilateral ligation of common carotid artery (CCA) group (UL group) and bilateral ligation of CCA group (BL group). Rabbits were cervix—cut to expose the arteries without ligation (sham group), with right—side ligation (UL group) and bilateral ligation (BL group), respectively.