Recent advances in data collection for Cryo-EM methods.

Methods of transmission electron microscopy (TEM) are typically used to resolve structures of vitrified biological specimens using both single particle analysis (SPA) and tomographic methods and use both conventional as well as scanning transmission modes of data collection. Automation of data collection for each method has been developed to different levels of convenience for the users. Automation of methods using the conventional TEM mode has progressed the furthest.