1,107 results match your criteria: "Centre of the Czech Academy of Sciences[Affiliation]"
Folia Parasitol (Praha)
January 2023
Faculty of Agriculture and Technology, University of South Bohemia in Ceske Budejovice, Czech Republic.
A total of 1,724 beef and 2,941 dairy cattle older than one year from 66 beef and 67 dairy farms in the Czech Republic were examined for the presence of rumen and liver fluke eggs in 2019-2022. Out of 227 positive animals, all were positive for paramphistome and five for fasciolid eggs. Molecular analysis of the ITS2 rDNA revealed the presence of Calicophoron daubneyi (Dinnik, 1962) and Fasciola hepatica Linnaeus, 1758.
View Article and Find Full Text PDFFolia Parasitol (Praha)
December 2022
Department of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Iraq.
Recent examinations of some marine fishes from off the southern coast of Iraq revealed the presence of five species of Philometra Costa, 1845 (Nematoda: Philometridae): Philometra arabiensis sp. n. (males and females) from the ovary of the shrimp scad Alepes djedaba (Forsskål) (Carangidae, Carangiformes), Philometra psettoditis Moravec, Walter et Yuniar, 2012 (females) from the body cavity of the Indian halibut Psettodes erumei (Bloch et Schneider) (Psettodidae, Pleuronectiformes), Philometra terapontis Moravec, Gopalakrishnan, Rajkumar, Saravanakumar et Kaliyamoorthy, 2011 (female) from the ovary of the jarbua terapon Terapon jarbua (Forsskål) (Terapontidae, Centrarchiformes), Philometra sp.
View Article and Find Full Text PDFSci Total Environ
March 2023
Biology Centre of the Czech Academy of Sciences, Institute of Hydrobiology, Na Sádkách 7, České Budějovice 37005, Czech Republic. Electronic address:
To examine the suitability of fish scales as potential tracers of nutrient pollution, we analysed the nitrogen and carbon stable isotope values (δN and δC) in scales of a generalist fish species, roach Rutilus rutilus, collected from 22 Czech reservoirs covering wide gradients of catchment land use and nutrient enrichment. Using generalised additive mixed models in the first step and generalised linear mixed models in the second step, we evaluated the response of roach scale stable isotope values to catchment land use variables (percentage of agricultural land and human population density) and in-reservoir water quality variables. Roach scale δN values varied by 15 ‰ among the reservoirs and were strongly, linearly, and positively associated with the percentage of agricultural land in the reservoir catchments, pointing to agriculture as the dominant source of nitrogen pollution in the investigated systems.
View Article and Find Full Text PDFSci Rep
December 2022
Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
Indian cultural influence is remarkable in present-day Mainland Southeast Asia (MSEA), and it may have stimulated early state formation in the region. Various present-day populations in MSEA harbor a low level of South Asian ancestry, but previous studies failed to detect such ancestry in any ancient individual from MSEA. In this study, we discovered a substantial level of South Asian admixture (ca.
View Article and Find Full Text PDFAntiviral Res
January 2023
Department of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland. Electronic address:
Tick-borne encephalitis virus (TBEV) is a major cause of neurological infections in many regions of central, eastern and northern Europe and northern Asia. In approximately 15% of cases, TBEV infections lead to the development of severe encephalitis or meningitis. The main route of TBEV transmission is tick bites; however, ingestion of dairy products from infected animals (goats, cattle and sheep) is also a frequent cause of the disease.
View Article and Find Full Text PDFFront Microbiol
December 2022
Limnological Station, Department of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
Planktonic ciliate species form multiple trophic guilds and are central components of freshwater food webs. Progress in molecular analytical tools has opened new insight into ciliate assemblages. However, high and variable 18S rDNA copy numbers, typical for ciliates, make reliable quantification by amplicon sequencing extremely difficult.
View Article and Find Full Text PDFAntiviral Res
February 2023
Laboratory of Emerging Viral Diseases, Veterinary Research Institute, Brno, Czech Republic; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic; Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address:
Tick-borne encephalitis (TBE) is a potentially fatal disease common in much of Europe and Asia. There is no specific therapy for the treatment of TBE patients. However, several efforts are being made to develop small molecules that specifically interfere with the life cycle of TBE virus.
View Article and Find Full Text PDFMicroorganisms
November 2022
Department of Food Preservation, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 166 28 Prague, Czech Republic.
The protective effect of bacteriophage suspensions (Ds3CZ + Ds20CZ and PcCB7V + PcCB251) on phytopathogenic bacteria causing soft rot of potato tubers, namely Dickeya dianthicola (D50, D200) and Pectobacterium carotovorum (P87, P224), was observed in ex vivo and in vitro experiments. Ex vivo tests were performed (with air access) on potato slices, on cylindrical cuts from the center of the tubers, and directly in whole potato tubers. In vitro experiments were carried out in a liquid medium using RTS-8 bioreactors, where bacterial growth was monitored as optical density.
View Article and Find Full Text PDFNucleic Acids Res
January 2023
Department of Cell Biology and Radiobiology, Institute of Biophysics of the Czech Academy of Sciences, Brno CZ-61265, Czech Republic.
In contrast to the catalytic subunit of telomerase, its RNA subunit (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin of TRs transcribed with RNA polymerase II in Opisthokonta (the supergroup including Animalia and Fungi) and Trypanosomida on one hand, and TRs transcribed with RNA polymerase III under the control of type 3 promoter, found in TSAR and Archaeplastida supergroups (including e.g.
View Article and Find Full Text PDFVirus Res
January 2023
Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, CZ-62100 Brno, Czechia; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, CZ-37005 Ceske Budejovice, Czechia; Department of Experimental Biology, Faculty of Science, Masaryk University, CZ-62500 Brno, Czechia. Electronic address:
Matrix metalloproteinases (MMPs) play an important role in central nervous system infections. We analysed the levels of 8 different MMPs in the cerebrospinal fluid (CSF) of 89 adult patients infected with tick-borne encephalitis (TBE) virus and compared them with the levels in a control group. MMP-9 was the only MMP that showed significantly increased CSF levels in TBE patients.
View Article and Find Full Text PDFFront Mol Biosci
November 2022
Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czechia.
Environ Microbiol Rep
February 2023
Department of Fisheries Oceanography and Marine Ecology, National Marine Fisheries Research Institute, Gdynia, Poland.
Aerobic anoxygenic photoheterotrophic (AAP) bacteria represent a functional group of prokaryotic organisms that harvests light energy using bacteriochlorophyll-containing photosynthetic reaction centers. They represent an active and rapidly growing component of freshwater bacterioplankton, with the highest numbers observed usually in summer. Species diversity of freshwater AAP bacteria has been studied before in lakes, but its seasonal dynamics remain unknown.
View Article and Find Full Text PDFFront Microbiol
November 2022
Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Collembola are a group of globally distributed microarthropods that can tolerate low temperature and are active in extremely cold environments. While it is well known that animal diets can shape their microbiota, the microbiota of soil animals is not well described, particularly for animals with limited food resources, such as Collembola active in winter at low temperatures. In this study, we explored the effects of three different food sources; corn litter (agriculture grain residuals), Mongolian oak litter (natural plant residuals), and yeast (common food for Collembola culture), on the microbiota of a winter-active Collembola species, .
View Article and Find Full Text PDFAdv Healthc Mater
March 2023
Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkynova 123, Brno, CZ-61200, Czech Republic.
Int J Mol Sci
November 2022
Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Studničkova 7, 128 00 Prague, Czech Republic.
are of great interest in the pharmaceutical industry as they produce a plethora of secondary metabolites that act as antibacterial and antifungal agents. They may thrive on their own in the soil, or associate with other organisms, such as plants or invertebrates. Some soil-derived strains exhibit hemolytic properties when cultivated on blood agar, raising the question of whether hemolysis could be a virulence factor of the bacteria.
View Article and Find Full Text PDFbioRxiv
November 2022
Institute for Research in Biomedicine, Università della Svizzera italiana; Bellinzona, Switzerland.
Unlabelled: Emergence of SARS-CoV-2 variants diminishes the efficacy of vaccines and antiviral monoclonal antibodies. Continued development of immunotherapies and vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions of the virus spike that are both functionally relevant and averse to change, we identified human neutralizing antibodies to highly conserved viral epitopes.
View Article and Find Full Text PDFJ Anim Ecol
February 2023
Key Laboratory for Earth Surface Processes of the Ministry of Education, Institute of Ecology, College of Urban and Environmental Science, Peking University, Beijing, China.
The ecological stoichiometry theory provides a framework to understand organism fitness and population dynamics based on stoichiometric mismatch between organisms and their resources. Recent studies have revealed that different soil animals occupy distinct multidimensional stoichiometric niches (MSNs), which likely determine their specific stoichiometric mismatches and population responses facing resource changes. The goals of the present study are to examine how long-term forest plantations affect multidimensional elemental contents of litter and detritivores and the population size of detritivores that occupy distinct MSNs.
View Article and Find Full Text PDFSci Total Environ
February 2023
Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology and SoWa RI, Na Sádkách 7, České Budějovice CZ-37005, Czech Republic.
The predicted global increase in the frequency, severity, and intensity of forest fires includes Central Europe, which is not currently considered as a wildfire hotspot. Because of this, a detailed knowledge of long-term post-fire forest floor succession is essential for understanding the role of wildfires in Central European temperate forests. In this study, we used a space-for-time substitution approach and exploited a unique opportunity to observe successional changes in the physical, chemical, and microbial properties of the forest floor in coniferous forest stands on a chronosequence up to 110 years after fire.
View Article and Find Full Text PDFJ Nematol
February 2022
CABI Switzerland, Delémont, Switzerland.
Alternatives to hazardous insecticides are urgently needed for an environmentally friendly and effective management of insect pests. One such option is the use of entomopathogenic nematodes (EPN). To increase the availability of EPN with potential for biocontrol, we surveyed agricultural soils in the Republic of Rwanda and collected two isolates.
View Article and Find Full Text PDFCommun Biol
November 2022
Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic.
Urol Oncol
February 2023
Department of Urology, 3rd Faculty of Medicine, Charles University, Prague and Thomayer University Hospital, Prague, Czech Republic.
Introduction: Human urine microbiota (UM) research has uncovered associations between composition of microbial communities of the lower urinary tract and various disease states including several reports on the putative link between UM and bladder cancer (BC). The aim of this study was to investigate male UM in patients with BC and controls using catheterised urine specimens unlike in previous studies.
Methods: Urine samples were obtained in theatre after surgical prepping and draping using aseptic catheterisation.
Mitochondrion
January 2023
Laboratory for Metabolism and Bioenergetics, Department of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Czech Republic. Electronic address:
Triphenylphosphonium (TPP) derivatives are commonly used to target chemical into mitochondria. We show that alkyl-TPP cause reversible, dose- and hydrophobicity-dependent alterations of mitochondrial morphology and function and a selective decrease of mitochondrial inner membrane proteins including subunits of the respiratory chain complexes, as well as components of the mitochondrial calcium uniporter complex. The treatment with alkyl-TPP resulted in the cleavage of the pro-fusion and cristae organisation regulator Optic atrophy-1.
View Article and Find Full Text PDFJ Evol Biol
January 2023
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic.
The interactions of evolutionary forces are difficult to analyse in free-living populations. However, when properly understood, they provide valuable insights into evolutionary biology and conservation genetics. This is particularly important for the interplay of genetic drift and natural selection in immune genes that confer resistance to disease.
View Article and Find Full Text PDFInsects
October 2022
Department of Forest Ecology, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 613 00 Brno, Czech Republic.
Taking the regular overwintering of spider species in land snail shells as a model, we studied environmental conditions affecting the choice of overwintering sites in three jumping spider species: , , and . The research was conducted at 11 steppe localities on calcareous bedrock with abundant empty shells (mainly and ). We documented 889 shells and collected 186 of them, of which 113 were inhabited by 146 spider individuals (13 species).
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
February 2023
University of Belgrade - Faculty of Agriculture, Nemanjina 6, Zemun, 11080, Belgrade, Serbia.
Atmospheric pollution remains one of the growing concerns in the twenty-first century, with particular focus on metal trace elements (MTE) from anthropogenic sources, due to their adverse effects on biota. The concentration and type of MTE in the atmosphere and in the soil are diverse, depending on the origin of pollutants, which can cause diverse detrimental effects on organisms living in the nearby environment. Three sites in Central Serbia with different origins of MTE pollution (urban contamination, smelting, and fly ash area) were assessed, using terrestrial Roman snails (Helix pomatia) as biomarker organisms.
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