Quassinoids as Promising Anti-cancer Agents.

The use of current anticancer drugs is hampered by significant side effects and high costs. In the pursuit of safer, more effective, and affordable options, researchers have turned to nature as a valuable source of potential anticancer compounds. Quassinoids, a class of natural terpenoids, have garnered attention for their anticancer properties.

Interfacial Thermoconvection and Atomic Relay Catalysis Enable Equilibrium Shifting and Rapid Glucose-to-Fructose Isomerization.

The aqueous glucose-to-fructose isomerization is controlled by thermodynamics to an equilibrium limit of ~50 % fructose yield. However, here we report an in situ fructose removal strategy enabled by an interfacial local photothermal effect in combination with relay catalysis of geminal and isolated potassium single atoms (K SAs) on graphene-type carbon (K/GT) to effectively bypass the equilibrium limit and markedly speed up glucose-to-fructose isomerization. At 25 °C, an unprecedented fructose yield of 68.

Synergistic approach for enhanced production of polyhydroxybutyrate by Bacillus pseudomycoides SAS-B1: Effective utilization of glycerol and acrylic acid through fed-batch fermentation and its environmental impact assessment.

The continual rise in the consumption of petroleum-based synthetic polymers raised a significant environmental concern. Bacillus pseudomycoides SAS-B1 is a gram-positive rod-shaped halophilic bacterium capable of accumulating Polyhydroxybutyrate (PHB)-an intracellular biodegradable polymer. In the present study, the optimal conditions for cell cultivation in the seed media were developed.

Production and characterization of bioactive peptides in fermented soybean meal produced using proteolytic Bacillus species isolated from kinema.

The study aims to enhance the functional properties of soybean meal (SBM) using potent proteolytic Bacillus strains isolated from kinema, a traditional fermented soybean product of Sikkim Himalaya. Selected Bacillus species; Bacillus licheniformis KN1G, B. amyloliquifaciens KN2G, B.

In-silico analysis of a halophilic bacterial isolate-Bacillus pseudomycoides SAS-B1 and its polyhydroxybutyrate production through fed-batch approach under differential salt conditions.

Polyhydroxybutyrate (PHB) is a natural biopolymer and a viable substitute for petroleum-derived polymers that possess immense potential for diverse applications. In the present study, PHB was produced by a halophilic bacteria identified as Bacillus pseudomycoides SAS-B1 by 16S rRNA gene sequencing. The bacterial genome was evaluated through complete genome sequencing, which elucidated a 5,338,308 bp genome with 34.

Plant Growth-Promoting Attributes of Zinc Solubilizing Dietzia maris Isolated from Polyhouse Rhizospheric Soil of Punjab.

Zinc solubilizing rhizobacteria (ZSR) enhance the phyto-availability of Zn by converting its insoluble forms into usable forms that are essential for the growth and nutritional quality of crops. In the present study, a potential ZSR, hereafter referred to as strain N14, was isolated from the polyhouse rhizospheric soil of Punjab, India. The isolated rhizobacteria was found to be Gram-positive, aerobic, rod-shaped, and demonstrated a solubilization index of 63.

Genomic analysis, simultaneous production, and process optimization of extracellular polymeric substances and polyhydroxyalkanoates by Methylobacterium sp. ISTM1 by utilizing molasses.

In the current study, the isolated Methylobacterium sp. ISTM1 simultaneously produced both extracellular polymeric substances (EPS) and polyhydroxyalkanoates (PHA) in a single-step process. The yield of biopolymers (EPS and PHA) was enhanced by optimizing the process parameters of EPS and PHA production.

Peptide candidates for the development of therapeutics and vaccines against β-coronavirus infection.

Betacoronaviruses (β-CoVs) have caused major viral outbreaks in the last two decades in the world. The mutation and recombination abilities in β-CoVs resulted in zoonotic diseases in humans. Proteins responsible for viral attachment and replication are highly conserved in β-CoVs.

Production and characterization of bioactive peptides from rice beans using Bacillus subtilis.

A bioprocess was developed for production of bioactive peptides on microbial fermentation of rice beans using proteolytic Bacillus subtilis strains. The peptides produced were identified by LC-MS/MS analysis, revealing the presence of many unique peptide sequences to individual hydrolysates. On functional properties prediction, antihypertensive peptides (3.

Biotechnological potential of psychrophilic microorganisms as the source of cold-active enzymes in food processing applications.

Microorganisms striving in extreme environments and exhibiting optimal growth and reproduction at low temperatures, otherwise known as psychrophilic microorganisms, are potential sources of cold-active enzymes. Owing to higher stability and cold activity, these enzymes are gaining enormous attention in numerous industrial bioprocesses. Applications of several cold-active enzymes have been established in the food industry, e.

Recent approaches for enhanced production of microbial polyhydroxybutyrate: Preparation of biocomposites and applications.

In modern decades, an increase in environmental awareness has attracted the keen interest of researchers to investigate eco-sustainable, recyclable materials to minimize reliance on petroleum-based polymeric compounds. Poly (3-hydroxybutyrate) is amorphous, linear, and biodegradable bacterial polyesters that belong to the polyhydroxyalkanoates family with enormous applications in many fields. The present review provides comprehensive information on polyhydroxybutyrate production from different biomass feedstock.

A Multifunctional Peptide From Fermented Soybean for Effective Inhibition of SARS-CoV-2 S1 Receptor Binding Domain and Modulation of Toll Like Receptor 4: A Molecular Docking Study.

Fermented soybean products are traditionally consumed and popular in many Asian countries and the northeastern part of India. To search for potential agents for the interruption of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike glycoprotein 1 (S1) and human angiotensin-converting enzyme 2 (ACE2) receptor interactions, the antiviral prospective of peptides identified from the proteome of was investigated. Soybean was fermented using KN1G, KN2G and two different strains of (KN2B and KN2M).

BiWO/C-Dots/TiO: A Novel Z-Scheme Photocatalyst for the Degradation of Fluoroquinolone Levofloxacin from Aqueous Medium.

Photocatalytic materials and semiconductors of appropriate structural and morphological architectures as well as energy band gaps are materials needed for mitigating current environmental problems, as these materials have the ability to exploit the full spectrum of solar light in several applications. Thus, constructing a Z-scheme heterojunction is an ideal approach to overcoming the limitations of a single component or traditional heterogeneous catalysts for the competent removal of organic chemicals present in wastewater, to mention just one of the areas of application. A Z-scheme catalyst possesses many attributes, including enhanced light-harvesting capacity, strong redox ability and different oxidation and reduction positions.

A critical review on current strategies and trends employed for removal of inhibitors and toxic materials generated during biomass pretreatment.

The main objective of biomass pretreatment is to separate biomass components and provide easier access with ultimate aim for lignin removal, hemicellulose protection and cellulose crystallinity reduction. Effective bioconversion with least inhibitory compound production would play a considerable role in economic practicability of the process in order to achieve economic sustainability. In this regard, detoxification is an important condition to make biomass hydrolysate acquiescent to bioconversion; also, understanding of inhibitors effect on growth and fermentation are necessary requirements for system detoxification.

Identification of markers at various stages of batch fermentation and improved production of xylanase using (KP874102.1).

Improved xylanase production was carried out through optimization of environmental stresses during spore preservation, seed cultivation and batch fermentation and identifies the markers at various stages. The maximum spore size (radius 6.5 µm) of was noticed after 28 days of spore preservation.

De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism.

Background: Aloe vera is a perennial, succulent, drought-resistant plant that exhibits many pharmacological characteristics such as wound healing ability against skin burns, anti-ulcer, anti-inflammatory, anti-tumor, anti-viral, anti-hypercholesterolemic, anti-hyperglycemic, anti-asthmatic and much more. Despite great medicinal worth, little genomic information is available on Aloe vera. This study is an initiative to explore the full-scale functional genomics of Aloe vera by generating whole transcriptome sequence database, using Illumina HiSeq technology and its progressive annotation specifically with respect to the metabolic specificity of the plant.

Biocatalytic Synthesis of Novel Partial Esters of a Bioactive Dihydroxy 4-Methylcoumarin by Rhizopus oryzae Lipase (ROL).

Highly regioselective acylation has been observed in 7,8-dihydroxy-4-methylcoumarin (DHMC) by the lipase from suspended in tetrahydrofuran (THF) at 45 °C using six different acid anhydrides as acylating agents. The acylation occurred regioselectively at one of the two hydroxy groups of the coumarin moiety resulting in the formation of 8-acyloxy-7-hydroxy-4-methylcoumarins, which are important bioactive molecules for studying biotansformations in animals, and are otherwise very difficult to obtain by only chemical steps. Six monoacylated, monohydroxy 4-methylcoumarins have been biocatalytically synthesised and identified on the basis of their spectral data and X-ray crystal analysis.

Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties.

Alkaline protease from brinjal leaf () having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 U/mg.

Light and auxin responsive cytochrome P450s from Withania somnifera Dunal: cloning, expression and molecular modelling of two pairs of homologue genes with differential regulation.

Cytochrome P450s (CYPs) catalyse a wide variety of oxygenation/hydroxylation reactions that facilitate diverse metabolic functions in plants. Specific CYP families are essential for the biosynthesis of species-specialized metabolites. Therefore, we investigated the role of different CYPs related to secondary metabolism in Withania somnifera, a medicinally important plant of the Indian subcontinent.

Molecular cloning and biochemical characterization of a recombinant sterol 3-O-glucosyltransferase from Gymnema sylvestre R.Br. catalyzing biosynthesis of steryl glucosides.

Gymnema sylvestre R.Br., a pharmacologically important herb vernacularly called Gur-Mar (sugar eliminator), is widely known for its antidiabetic action.