Adipokine protein expression pattern in growth hormone deficiency predisposes to the increased fat cell size and the whole body metabolic derangements.

Context: GH deficiency (GHD) in adults is associated with central adiposity, dyslipidemia, and insulin resistance.

Objective: The objective of the study was to test the hypothesis that GHD might change the spectrum of adipokines and thus influence the adipose tissue and the whole-body metabolic and inflammatory status leading to development of insulin resistance.

Design: This was a single-center observational study with a cross-sectional design.

Cell volume and peptide hormone secretion.

In general way cell swelling evokes and shrinking inhibits exocytosis of proteins and peptides stored in secretory vesicles from various types of cells. Dynamics of this type of hormone secretion is indistinguishable from that induced by specific secretagogue. Peculiarities of swelling-induced secretion indicate an involvement of the unique signaling pathway.

Different signaling pathways involved in glucose- and cell swelling-induced insulin secretion by rat pancreatic islets in vitro.

Background: The objective was to compare signal transduction pathways exploited by glucose and cell swelling in stimulating insulin secretion.

Methods: Isolated rat (Wistar) pancreatic islets were stimulated in vitro by 20 mmol/l glucose or 30% hypotonic medium (202 mOsm/kg) in various experimental conditions.

Results: Glucose did not stimulate insulin release in calcium free medium.