Identification of Monilinia fructigena, M. fructicola, M. laxa, and Monilia polystroma on Inoculated and Naturally Infected Fruit Using Multiplex PCR.

Monilinia fructigena, M. fructicola, M. laxa, and Monilia polystroma each have a different regulatory status.

Variations in sequence and occurrence of SSU rDNA group I introns in Monilinia fructicola isolates.

A group I intron of 418 base pairs in the Monilinia fructicola ribosomal small-subunit sequence was characterized. The absence of such an intron in M. laxa and M.

Monoclonal antibodies specific for Campylobacter fetus lipopolysaccharides.

Four monoclonal antibodies (mAbs) (M1357, M1360, M1823 and M1825) which reacted with Campylobacter fetus lipopolysaccharide (LPS) core region epitopes were produced and characterized. Reactivity of these mAbs with C. fetus core LPS epitopes was determined by enzyme-linked immunosorbent assay (ELISA) with whole cell proteinase K digests and phenol-water extracted LPS, and by immunoblotting with proteinase K digests.

Relationship between egg size and subgroup J avian leukosis virus in eggs from broiler breeders.

Hatching eggs from three broiler breeder flocks that had experienced losses from myeloid leukosis were tested for infection with avian leukosis virus of subgroup J (ALV-J). Sufficient eggs were positive in two flocks to relate infection to egg weight. Allantoic fluid, embryonic tissue and yolk were collected after 18 days of incubation.

Differentiation of Tilletia Species by rep-PCR Genomic Fingerprinting.

The potential of repetitive-sequence-based polymerase chain reaction (rep-PCR) fingerprinting of fungal genomic DNA as a rapid and simple alternative to random amplified polymorphic DNA (RAPD) analysis in the study of phylogenetic relationships, and also as a diagnostic method, was investigated with species of Tilletia. DNA primers (BOX, ERIC, and REP) corresponding to conserved repetitive element motifs, originally described in prokaryotes, were used to generate genomic fingerprints of T. indica, T.