Temperature and nitrogen effects on regulators and products of the flavonoid pathway: experimental and kinetic model studies.

The flavonoid pathway is known to be up-regulated by different environmental stress factors. Down-regulation of the pathway is much less studied and is emphasized in the present work. Flavonoid accumulation was induced by exposing plants for 1 week to nitrogen depletion at 10 degrees C, giving high levels of anthocyanins and 3-glucoside-7-rhamnosides, 3,7-di-rhamnosides and 3-rutinoside-7-rhamnosides of kaempferol and quercetin.

Signalling cascades integrating light-enhanced nitrate metabolism.

In higher plants, light is crucial for regulation of nitrate uptake, translocation and assimilation into organic compounds. Part of this metabolism is tightly coupled to photosynthesis because the enzymes involved, nitrite reductase and glutamate synthase, are localized to the chloroplasts and receive reducing power from photosynthetic electron transport. However, important enzymes in nitrate acquisition and reduction are localized to cellular compartments other than chloroplasts and are also up-regulated by light, i.

Interdependency of formation and localisation of the Min complex controls symmetric plastid division.

Plastid division represents a fundamental biological process essential for plant development; however, the molecular basis of symmetric plastid division is unclear. AtMinE1 plays a pivotal role in selection of the plastid division site in concert with AtMinD1. AtMinE1 localises to discrete foci in chloroplasts and interacts with AtMinD1, which shows a similar localisation pattern.

Plastid division in an evolutionary context.

Plastids are derived from free-living cyanobacteria that were engulfed by eukaryotic host cells through the process of endosymbiosis and, like their cyanobacterial ancestors, divide by binary fission. Over the last decade the continued identification and functional analysis of plastid division components, coupled with ever-increasing genomic resources, have yielded insights into the origins and evolution of the plastid division mechanism in higher plants. Here we review the current understanding of the evolution of the chloroplast division proteins and present a model of how the machinery has developed to execute plastid division in Arabidopsis.

Plastid division coordination across a double-membraned structure.

Chloroplasts still retain components of the bacterial cell division machinery and research over the past decade has led to an understanding of how these stromal division proteins assemble and function as a complex chloroplast division machinery. However, during evolution plant chloroplasts have acquired a number of cytosolic division proteins, indicating that unlike the cyanobacterial ancestors of plastids, chloroplast division in higher plants require a second division machinery located on the chloroplast outer envelope membrane. Here we review the current understanding of the stromal and cytosolic plastid division machineries and speculate how two protein machineries coordinate their activities across a double-membraned structure.