Fatty Acids of European Sardine () White Muscle Can Discriminate Geographic Origin Along the Iberian Atlantic Coast.

The European sardine () ranks among the most valuable species of Iberian fisheries, and the accurate tracing of its geographic origin, once landed, is paramount to securing sustainable management of fishing stocks and discouraging fraudulent practices of illegal, unreported, and unregulated (IUU) fishing. The present study investigated the potential use of white muscle fatty acids (FAs) to successfully discriminate the geographic origin of samples obtained in seven commercially important fishing harbors along the Iberian Atlantic Coast. While 35 FAs were identified using gas chromatography-mass spectrometry in the white muscle of , the following, as determined by the Boruta algorithm, were key for sample discrimination: 14:0, 22:6-3, 22:5-3, 18:0, 20:5-3, 16:1-7, 16:0, and 18:1-7 (in increasing order of relevance).

Is Silver a Precious Metal for G-Quadruplex Stabilization Mediated by Porphyrins?

Cancer is a leading cause of death, so continuous efforts into cancer therapy are imperative. In tumor cells, telomerase and oncogene activity are key points for uncontrolled cell growth. Targeting these processes with ligands that inhibit telomerase and/or reduce oncogene expression has been identified as a promising cancer therapy.

Transplantation of seagrass (Zostera noltei) as a potential nature-based solution for the restoration of historically contaminated mudflats.

Within the UN Decade on Ecosystem Restoration (2021-2030) framework, a Nature-based Solution (NbS) using Zostera noltei transplants was tested to restore a historically contaminated intertidal area. In-situ transplantation relied on patches of seagrass and sediment from a Donor meadow and its evolution was monitored for two years. The evaluation of the transplant success encompassed the seagrass coverage area, seagrass biomass, tissue mercury (Hg) accumulation, and photosynthetic efficiency.

A critical review of microplastics characterisation in aquatic environments: recent trends in the last 10 years.

Anthropogenic activities have introduced various contaminants into freshwater and marine ecosystems. Microplastics (MPs) are persistent and ubiquitous contaminants threatening natural ecosystems and impairing organisms at different biological levels of organization. Their durability and degradation rate pose a great concern in the scientific community, and thus, several techniques have been used to detect MPs effectively.

Integrons are key players in the spread of beta-lactamase-encoding genes.

Integrons mediate the acquisition and expression of gene cassettes (GCs). The production of beta-lactamases (BLs) is the most relevant mechanism of beta-lactams resistance. To explore the role of integrons in BL genes dissemination, we retrieved sequences and metadata from the INTEGRALL database and performed literature review.

Unravelling a Latent Pathobiome Across Coral Reef Biotopes.

Previous studies on disease in coral reef organisms have neglected the natural distribution of potential pathogens and the genetic factors that underlie disease incidence. This study explores the intricate associations between hosts, microbial communities, putative pathogens, antibiotic resistance genes (ARGs) and virulence factors (VFs) across diverse coral reef biotopes. We observed a substantial compositional overlap of putative bacterial pathogens, VFs and ARGs across biotopes, consistent with the 'everything is everywhere, but the environment selects' hypothesis.

Effects of wildfire ash on the fatty acid and sugar profiles of bivalves - A comparative study of a freshwater and a marine species.

Wildfires can impact both freshwater and marine ecosystems through post-fire runoff, but its effects on bivalves, particularly those living in marine habitats, remain largely overlooked. While evidence exists that wildfire ash can alter the fatty acid (FA) and sugar profiles of aquatic biota, its influence on the biochemical profiles of bivalves have not been addressed to date. This study aimed to assess the effects of ash exposure on the FA and sugar profiles of two bivalve species used for human consumption: a freshwater clam (Corbicula fluminea) and a marine bivalve (Cerastoderma edule), additionally evaluating potential effects on their nutritional value.

Revealing hidden risks: in vitro analysis of PFAS hazards in Mytilus galloprovincialis gills and digestive gland.

Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals known for their persistence and bioaccumulation, leading to widespread environmental contamination. Despite their recognised environmental risks, particularly to aquatic wildlife, including marine invertebrates, detailed impact studies are limited. PFAS can be categorised according to the length of the compound chain, with short-chain PFAS announced as a safer alternative to the more commonly used long-chain PFAS.

Combined effects of fluoroquinolone antibiotic enrofloxacin and rising sea temperatures on the health of the Mediterranean mussel (Mytilus galloprovincialis): Exploring physiological, biochemical, and energetic balance dynamics.

Human activity exposes organisms in marine ecosystems to numerous stressors, including rising seawater temperatures and antibiotic contamination. The present study investigated the impacts of environmentally relevant concentrations of the fluoroquinolone (FQ) antibiotic enrofloxacin (ENR), specifically 5 and 500 ng/L, in Mytilus galloprovincialis under ambient (20 °C) and predicted warming (25 °C) conditions after 14 days of exposure, followed by a 14-day recovery period in the absence of ENR. The chemical analyses revealed significant variability in bioaccumulation in mussel tissues.

Sensing in Inland Waters to Promote Safe Navigation: A Case Study in the Aveiro's Lagoon.

Maritime navigation safety relies on preventing accidents, such as collisions and groundings. However, several factors can exacerbate these risks, including inexistent or inadequate buoyage systems and nautical charts with outdated bathymetry. The International Hydrographic Organization (IHO) highlights high costs and traditional methods as obstacles to updating bathymetric information, impacting both safety and socio-economic factors.

Low-Cost Sensor Network for Air Quality Assessment in Cabo Verde Islands.

This study explores the application of low-cost sensor networks for air quality monitoring in Cabo Verde islands, utilizing Clarity Node-S sensors to measure fine particulate matter with diameters equal to or smaller than 10 µm (PM10) and 2.5 µm (PM2.5) and nitrogen dioxide (NO) gasses, across various locations.

Impact of and Extracts on UVA-Irradiated on 3D Cultured Melanoma Cells: A Proteomic Insight.

Melanoma is one of the most malignant forms of skin cancer, characterised by the highest mortality rate among affected patients. This study aims to analyse and compare the effects of lipid extracts from the microalgae () and () on the intra and extracellular proteome of UVA-irradiated melanoma cells using a three-dimensional model. Proteomic analysis revealed that UVA radiation significantly increases the levels of pro-inflammatory proteins in melanoma cells.

Short alkyl-chained Imidazolium-based Ionic Liquids: Promising green solution or potential environmental threat?

Ionic Liquids (ILs) are currently applied in a wide variety of fields, with promising outcomes in microalgae high value biocompounds extraction. The occurrence of these compounds in natural water systems, with their characteristic stability and low biodegradability, becomes a threat worthy of attention. In the present study, Dunaliella tertiolecta, Isochrysis galbana and Rhinomonas reticulata were exposed to 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([BMIM] TfN) for 72, 168 and 264 h, at 20 and 25 °C.

Effects of Directive 2010/63/EU on research on wild-sourced animals: a review of publication trends in studies on captive wild mice ( sp.).

Most animals used in experimentation are small mammals. In the EU, Directive 2010/63/EU regulates the use of laboratory animals for experimental purposes. However, there are few guidelines for the use of wild-sourced animals, which cover permits, experimentation, transport, maintenance, and setting free after experiments.

Organic Farming Enhances Diversity and Recruits Beneficial Soil Fungal Groups in Traditional Banana Plantations.

This study investigates the impact of organic (OF) and conventional farming (CF) on soil fungal communities in banana monoculture plantations on Madeira Island. We hypothesized that OF promotes beneficial fungal groups over harmful ones, sustaining soil health. Soil samples were collected from six plantations (three OF and three CF) for ITS amplicon sequencing to assess fungal diversity.

Isolation and Characterization of Infection of Four New Bacteriophages Infecting a Strain.

Bacteria from genus Vibrio continue to be one of the most common threats to aquaculture sustainability. spp. have been associated with infectious outbreaks in fish, shrimp, bivalves and even algae farms worldwide.

Plastisphere in a low-pollution mountain river: Influence of microplastics on survival of pathogenic bacteria.

Microplastics (MPs) are found even in remote and low-pollution freshwater ecosystems. However, the microbial communities associated with MPs in these environments remain poorly understood. We characterized the plastisphere in a low-pollution riverine ecosystem and evaluated the influence of different MPs in the persistence of pathogens in such environments.

Squid beaks as a proxy for mercury concentrations in muscle of the giant warty squid Moroteuthopsis longimana.

Cephalopods play a major role in marine food webs as both predators and prey. Although most of the Hg in cephalopods is present in the muscle, most studies on its accumulation by predators are based on concentrations in beaks. Here, using upper and lower beaks and buccal masses of Moroteuthopsis longimana, we evaluated the relationship between Hg concentrations in different cephalopod tissues.

Insect frass from upcycling vegetable by-products with cereals: Effects on the soil properties, plant development and soil invertebrate fitness.

The use of insects in organic management systems is expanding due to their ability to recycle waste into valuable co-products for agriculture, notably frass, constituted by the insect's excrements, larval exuviae, and remaining undigested feedstock. This study aimed to assess the effects of different application rates of frass (0.16, 0.

Novel PCR assay for the identification of two transmissible cancers in Cerastoderma edule.

Transmissible cancers are a rare phenomenon in which cancer cells are passed between individuals, leading to the development of neoplasia in the host organism. Transmissible cancers have been identified in three independent clonal lineages in mammals and eight different clonal lineages in bivalves. This study focused on the development of a multiplex PCR assay for the detection of two types of bivalve transmissible neoplasias (BTN) of cockles Cerastoderma edule (CedBTN1 and CedBTN2).

Electrospun Nanofiber Dopped with TiO and Carbon Quantum Dots for the Photocatalytic Degradation of Antibiotics.

Novel photocatalysts were synthesized through the association of carbon quantum dots (CQDs) with commercial (P25) titanium dioxide (TiO) by sonication. The resulting TiO/CQDs composite was then incorporated into the polyamide 66 (PA66) biopolymer nanofibers using the electrospinning technique, considering a composite nanoparticles-to-polymer ratio of 1:2 in the electrospinning precursor solution. The produced nanofibers presented suitable morphology and were tested for the photocatalytic degradation under simulated solar radiation of 10 mg L of amoxicillin (AMX) and sulfadiazine (SDZ), in phosphate buffer solution (pH 8.