Identification of peptide binding sequence of TRIM25 on 14-3-3σ by bioinformatics and biophysical techniques.

14-3-3σ protein is one of the seven isoforms from the highly conserved eukaryotic 14-3-3 protein family. Downregulation of 14-3-3σ expression has been observed in various tumors. TRIM25 is responsible for the proteolytic degradation of 14-3-3σ, in which abrogation of TRIM25 suppressed tumor growth through 14-3-3σ upregulation.

Cinnamic acid and lactobionic acid based nanoformulations as a potential antiamoebic therapeutics.

Acanthamoeba castellanii causes granulomatous amoebic encephalitis, an uncommon but severe brain infection and sight-threatening Acanthamoeba keratitis. Most of the currently used anti-amoebic treatments are not always effective, due to persistence of the cyst stage, and recurrence can occur. Here in this study we synthesize cinnamic acid and lactobionic acid-based magnetic nanoparticles (MNPs) using co-precipitation technique.

Corrigendum: Microbial identification, high-resolution microscopy and spectrometry of the rhizosphere in its native spatial context.

[This corrects the article DOI: 10.3389/fpls.2021.

Formation of a Nanorod-Assembled TiO Actinomorphic-Flower-like Microsphere Film via Ta Doping Using a Facile Solution Immersion Method for Humidity Sensing.

This study fabricated tantalum (Ta)-doped titanium dioxide with a unique nanorod-assembled actinomorphic-flower-like microsphere structured film. The Ta-doped TiO actinomorphic-flower-like microsphere (TAFM) was fabricated via the solution immersion method in a Schott bottle with a home-made improvised clamp. The samples were characterised using FESEM, HRTEM, XRD, Raman, XPS, and Hall effect measurements for their structural and electrical properties.

Effect of Wood Dust Fibre Treatments Reinforcement on the Properties of Recycled Polypropylene Composite (r-WoPPC) Filament for Fused Deposition Modelling (FDM).

The efficacy of wood dust fibre treatment on the property of wood dust reinforced recycled polypropylene composite (r-WoPPC) filament was investigated. The wood dust fibre was treated using alkali, silane, and NaOH-silane. The treated wood fibre was incorporated with r-PP using a twin-screw extruder to produce filament.

Simultaneous Method for Selected PBDEs and HBCDDs in Foodstuffs Using Gas Chromatography-Tandem Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry.

Flame retardants are added to consumer products to retard the ignition of combustible materials. Technical mixtures of polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCDD) were massively used for several decades. They are bioaccumulative, persistent, and have adverse effects on organisms.

Characterisation and substrate binding modes of exopolygalacturonase PGQ1 from .

Among the enzymes required for the efficient utilisation of pectin is polygalacturonase. harbours two polygalacturonases belonging to glycoside hydrolase family 28 (GH28). One of them, PGQ1, cleaved polygalacturonate exolytically at the non-reducing end into monomeric units.

Bioconversion of Used Transformer Oil into Polyhydroxyalkanoates by sp. Strain AAAID-1.5.

In this research, the utilisation of used transformer oil (UTO) as carbon feedstock for the production of polyhydroxyalkanoate (PHA) was targeted; with a view to reducing the environmental challenges associated with the disposal of the used oil and provision of an alternative to non-biodegradable synthetic plastic. sp. strain AAAID-1.

Impact of culture vessels on micro-morphological features of Sabin Blue orchid.

Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its microenvironment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.

Genome Sequence of sp. Strain CCB-PB317, Isolated from a Malaysian Mangrove, and Its Arsenic Resistance Mechanisms.

sp. strain CCB-PB317 with potential arsenic detoxification was isolated from a mangrove in Pulau Betong, Malaysia. Here, we report a draft genome sequence of strain CCB-PB317, which comprised 5,157,574 bp with a G+C content of 44.

Noncontact liquid-solid nanogenerators as self-powered droplet sensors.

Liquid-solid triboelectric nanogenerators (L-S TENGs) can generate corresponding electrical signal responses through the contact separation of droplets and dielectrics and have a wide range of applications in energy harvesting and self-powered sensing. However, the contact between the droplet and the electret will cause the contact L-S TENG's performance degradation or even failure. Here we report a noncontact triboelectric nanogenerator (NCLS-TENG) that can effectively sense droplet stimuli without contact with droplets and convert them into electrical energy or corresponding electrical signals.

Efficient Synthesis of DNA Duplexes Containing Reduced Acetaldehyde Interstrand Cross-Links.

DNA interstrand cross-links (ICLs) prevent DNA replication and transcription and can lead to potentially lethal events, such as cancer or bone marrow failure. ICLs are typically repaired by proteins within the Fanconi Anemia (FA) pathway, although the details of the pathway are not fully established. Methods to generate DNA containing ICLs are key to furthering the understanding of DNA cross-link repair.

Population genetic structure of the globally introduced big-headed ant in Taiwan.

Global commerce and transportation facilitate the spread of invasive species. The African big-headed ant, (Fabricius), has achieved worldwide distribution through globalization. Since the late 19th century, Taiwan has served as a major seaport because of its strategic location.

Nitric oxide and hydrogen sulfide work together to improve tolerance to salinity stress in wheat plants by upraising the AsA-GSH cycle.

The participation of nitric oxide (NO) in wheat plant tolerance to salinity stress (SS) brought about by hydrogen sulphide (HS) via modifying the ascorbate-glutathione (AsA-GSH) cycle was studied. The SS-plants received either 0.2 mM sodium hydrosulfide (NaHS; HS donor), or NaHS plus 0.

Functional analysis of germline RAD51C missense variants highlight the role of RAD51C in replication fork protection.

Monoallelic or biallelic RAD51C germline mutations results in chromosome instability disorders such as Fanconi anemia and cancers. The bona fide function of RAD51C is to assist RAD51 nucleoprotein filament onto single-strand DNA to complete homologous recombination (HR) repair. In addition to HR repair, the role of RAD51C in DNA replication is emerging when replication forks are transiently or irreversibly stalled.

Effect of Agar on the Mechanical, Thermal, and Moisture Absorption Properties of Thermoplastic Sago Starch Composites.

Thermoplastic starch is a material that has the potential to be environmentally friendly and biodegradable. However, it has certain drawbacks concerning its mechanical performance and is sensitive to the presence of moisture. The current study assessed agar-containing thermoplastic sago starch (TPSS) properties at various loadings.

Antiamoebic Properties of Ceftriaxone and Zinc-Oxide-Cyclodextrin-Conjugated Ceftriaxone.

is a ubiquitous free-living amoeba capable of instigating keratitis and granulomatous amoebic encephalitis in humans. Treatment remains limited and inconsistent. Accordingly, there is a pressing need for novel compounds.

The effect of root hairs on exudate composition: a comparative non-targeted metabolomics approach.

Root exudation is a major pathway of organic carbon input into soils. It affects soil physical properties, element solubility as well as speciation, and impacts the microbial community in the rhizosphere. Root exudates contain a large number of primary and secondary plant metabolites, and the amount and composition are highly variable depending on plant species and developmental stage.

Transcriptomic Signature of Horseshoe Crab Hemocytes' Response to Lipopolysaccharides.

() is one of the four species of horseshoe crabs (HSCs). The HSC hemocytes store defense molecules that are released upon encountering invading pathogens. The HSCs rely on this innate immunity to continue its existence as a living fossil for more than 480 million years.

Stable isotope labeling for detection of ozonation byproducts in effluent organic matter with FT-ICR-MS.

Despite effluent organic matter (EfOM) being a major consumer of ozone during wastewater treatment, little is known about ozonation byproducts (OBPs) produced from EfOM. To unambiguously identify OBPs, heavy ozone was used to ozonate EfOM, resulting in O labeled and unlabeled OBPs. Labeled OBPs mostly represent a single O transfer and were classified as either direct or indirect OBPs based on the O/O intensity ratios of the isotopologues.

Susceptibility of SARS Coronavirus-2 infection in domestic and wild animals: a systematic review.

Animals and viruses have constantly been co-evolving under natural circumstances and pandemic like situations. They harbour harmful viruses which can spread easily. In the recent times we have seen pandemic like situations being created as a result of the spread of deadly and fatal viruses.

Effect of Chemical Treatment of Sugar Palm Fibre on Rheological and Thermal Properties of the PLA Composites Filament for FDM 3D Printing.

The thermal and rheological properties of bio-composite filament materials are crucial characteristics in the development of a bio-composite Fused Deposition Modeling (FDM) filament since the printing mechanism of FDM strongly depends on the heating and extrusion process. The effect of chemical treatment on the thermal and rheological properties was investigated to develop composite filaments for FDM using natural fibres such as sugar palm fibre (SPF). SPF underwent alkaline and silane treatment processes before being reinforced with PLA for improving adhesion and removing impurities.

Origin of acetylcholine antagonism in ELIC, a bacterial pentameric ligand-gated ion channel.

ELIC is a prokaryotic homopentameric ligand-gated ion channel that is homologous to vertebrate nicotinic acetylcholine receptors. Acetylcholine binds to ELIC but fails to activate it, despite bringing about conformational changes indicative of activation. Instead, acetylcholine competitively inhibits agonist-activated ELIC currents.