Redistribution of fibronectin and keratin localization patterns in early wounded confluent PtK2 cells.

Single and double-label immunofluorescence were used to study the fibronectin (FN) and keratins (Ks) localization patterns in early wounded confluent PtK2 cells. A time-course study (0 hr, 2 hr, 6 hr and 24 hr) gives the following results: before wounding, the FN localizations of confluent cells are composed of curved and sometimes branched strands or fibrils. The Ks network is formed by radial fluorescent filaments connecting the Ks centers near the nuclei with a linear fluorescence underlying the cell membrane.

[In vitro proliferation of cells of the olivary region expressing macroglial and microglial markers].

Cells dissociated from the ventral medulla of embryonic or adult mice were cocultured on monolayers of an immortalized cerebellar astroglial clone. After 10-12 days, rare single cells gave rise to foci that formed colonies of 200,000 cells with a mixed macroglial and microglial phenotype.