75 results match your criteria: "Centre National de Référence des Streptocoques[Affiliation]"
Antibiotic therapy and prophylaxis of infective endocarditis - A SPILF-AEPEI position statement on the ESC 2023 guidelines.
Infect Dis Now
November 2024
Service des Maladies Infectieuses et Tropicales, Hôpital de Melun-Sénart, France.
The hypervirulent Group B Streptococcus HvgA adhesin promotes central nervous system invasion through transcellular crossing of the choroid plexus.
Fluids Barriers CNS
August 2024
Université Paris Cité, CNRS, Inserm, Institut Cochin, Paris, 22 rue Méchain, F-75014, France.
Article Synopsis
- Group B Streptococcus (GBS), particularly the hypervirulent CC17 clone, is a major cause of neonatal meningitis linked to significant mortality and disability.
- This study examined how GBS CC17 interacts with the choroid plexus, a critical part of the blood-cerebrospinal fluid barrier, using both mouse models and in vitro cell studies.
- Results showed that GBS CC17 effectively invades choroid plexus cells through a specific mechanism, leading to immune responses and potential pathways for brain infections.
Recurrent Group B Streptococcus neonatal invasive infections, France, 2007 -2021.
J Infect Dis
June 2024
Service de Bactériologie, Centre National de Référence des Streptocoques, Assistance Publique - Hôpitaux de Paris Centre, F-75014 Paris, France.
Article Synopsis
- A study on Group B Streptococcus (GBS) neonatal infections in France from 2007 to 2021 found that out of 1,527 cases, 36 (2.36%) were recurrent infections.
- Recurrence was more common in preterm (68%) and low birthweight (72%) infants, with a significant association to the hypervirulent GBS clonal complex 17.
- No tolerant strains were found, and genome sequencing didn't identify specific recurrence traits, highlighting the need for larger studies on managing these rare cases.
Performances of two rapid LAMP-based techniques for the intrapartum detection of Group B Streptococcus vaginal colonization.
Ann Clin Microbiol Antimicrob
April 2024
Université Paris Cité, CNRS, INSERM, Institut Cochin, Paris, F-75014, France.
Article Synopsis
- Group B Streptococcus (GBS) is a major cause of severe infections in newborns, and preventing these infections involves giving antibiotics to GBS-colonized pregnant women during labor.
- The study evaluated the effectiveness of two rapid tests, Ampliflash® GBS and PlusLife® GBS, for detecting GBS in vaginal samples, comparing them with traditional culture methods.
- Results showed that both tests were highly sensitive and specific, providing quick results within 10 to 35 minutes, indicating they could be more efficient in preventing GBS-related neonatal diseases than current screening methods, but further research is necessary.
Specific interaction between Group B CC17 hypervirulent clone and phagocytes.
Infect Immun
April 2024
Université Paris Cité, Institut Cochin, INSERM, U1016, CNRS, UMR8104, Paris, France.
Article Synopsis
- Group B Streptococcus (GBS), particularly the capsular serotype III called CC17, is a major cause of serious infections like bacteremia and meningitis in newborns, being labeled as a hypervirulent clone.
- The study found that CC17 strains are engulfed (phagocytosed) more effectively by human monocytes and macrophages compared to non-CC17 strains, due to enhanced attachment facilitated by specific proteins (HvgA and PI-2b pilus).
- Despite being phagocytosed more efficiently, both CC17 and non-CC17 strains survive similarly within immune cells, suggesting that CC17's enhanced phagocytosis might help it evade the immune system and persist in the
Outbreak of group B Streptococcus in a neonatal care unit confirmed by whole-genome sequencing.
Acta Paediatr
May 2024
Université Paris Cité, CNRS, INSERM, Institut Cochin, Paris, France.
Aim: Clusters of group B Streptococcus (GBS) infections in neonatal intensive care units (NICU) are poorly documented. We aimed to assess GBS cross-transmission during an outbreak of GBS sepsis.
Methods: The study was carried out between October and November 2021 in a French University Hospital.
A LAMP-based assay for the molecular detection of group B Streptococcus.
Eur J Clin Microbiol Infect Dis
October 2023
Service de Bactériologie-Virologie, CHRU de Tours, Université de Tours, 37044, Tours, France.
Purpose: Streptococcus agalactiae remains a major pathogen in human health, especially in neonatal infection. Detection in pregnant women is essential to initiate intrapartum antibiotic prophylaxis. This study compared the HiberGene loop-mediated isothermal amplification (LAMP) assay to culture, the reference method, for the detection of group B Streptococcus (GBS) in pregnant women.
View Article and Find Full Text PDFAuthor Correction: Microbial diversity in the vaginal microbiota and its link to pregnancy outcomes.
Sci Rep
August 2023
Institut Pasteur, Université Paris Cité, Département de biologie computationnelle, F-75015, Paris, France.
Characterization of TelE, a T7SS LXG Effector Exhibiting a Conserved C-Terminal Glycine Zipper Motif Required for Toxicity.
Microbiol Spectr
August 2023
Institut Pasteur, Université Paris Cité, CNRS UMR6047, Biology of Gram-positive Pathogens Unit, Paris, France.
Streptococcus gallolyticus subsp. () is an opportunistic bacterial pathogen strongly associated with colorectal cancer. Here, through comparative genomics analysis, we demonstrated that the genetic locus encoding the type VIIb secretion system (T7SSb) machinery is uniquely present in in two different arrangements.
View Article and Find Full Text PDFMicrobial diversity in the vaginal microbiota and its link to pregnancy outcomes.
Sci Rep
June 2023
Institut Pasteur, Université Paris Cité, Département de biologie computationnelle, F-75015, Paris, France.
Article Synopsis
- The vaginal microbiota consists of microorganisms in the vagina that play a crucial role in women's reproductive and overall health, primarily characterized by Lactobacillus species.
- Disruptions to the vaginal microbiota, caused by factors like antibiotics, sexual activity, and hormonal changes, can lead to conditions such as bacterial vaginosis, especially impactful during pregnancy.
- A study involving 749 pregnant women found that low diversity in the vaginal microbiota, particularly a depletion of Lactobacilli, is linked to an increased risk of preterm delivery, suggesting that overall diversity, rather than specific species, is a better risk indicator.
Microbiological Epidemiology of Invasive Infections Due to Non-Beta-Hemolytic Streptococci, France, 2021.
Microbiol Spectr
June 2023
Assistance Publique - Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre Site Cochin, Service de Bactériologie, Paris, France.
Article Synopsis
- - Non-beta-hemolytic streptococci (NBHS) are often overlooked as serious pathogens in invasive diseases, notably affecting older and immunocompromised individuals.
- - A study by the French National Reference Center for Streptococci revealed 522 invasive NBHS cases, with the most common types being Streptococcus anginosus, Streptococcus mitis, and Streptococcus sanguinis, and a notable proportion of infections led to severe conditions like bacteremia and endocarditis.
- - Many NBHS strains show resistance to commonly used antibiotics, especially beta-lactams, complicating treatment; however, they were generally susceptible to glycopeptides, highlighting the need for better detection methods for resistant strains
Early-onset neonatal sepsis in the Paris area: a population-based surveillance study from 2019 to 2021.
Arch Dis Child Fetal Neonatal Ed
March 2023
Assistance Publique-Hôpitaux de Paris, Hôpital Robert Debré, CNR Escherichia coli, Paris, France.
Background: Early-onset neonatal sepsis (EOS) is a rare condition but an important cause of severe morbidity and mortality in neonates.
Methods: This is a prospective observational study in neonates born at ≥34 weeks of gestation (WG). The primary endpoint was EOS, defined by isolation of pathogenic species from blood culture and/or cerebrospinal fluid culture within 72 hours after birth.
Type II Fatty Acid Synthesis Pathway and Cyclopropane Ring Formation Are Dispensable during Enterococcus faecalis Systemic Infection.
J Bacteriol
September 2021
Université de Paris, Institut Cochingrid.462098.1, INSERM, CNRS, Paris, France.
Enterococcus faecalis, a multiple antibiotic-resistant Gram-positive bacterium, has emerged as a serious nosocomial pathogen. Here, we used a genetic approach to characterize the strategies used by E. faecalis to fulfill its requirements for endogenous fatty acid (FA) synthesis and .
View Article and Find Full Text PDFCC17 group B Streptococcus exploits integrins for neonatal meningitis development.
J Clin Invest
March 2021
Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR8104, Paris, France.
Group B Streptococcus (GBS) is the major cause of human neonatal infections. A single clone, designated CC17-GBS, accounts for more than 80% of meningitis cases, the most severe form of the infection. However, the events allowing blood-borne GBS to penetrate the brain remain largely elusive.
View Article and Find Full Text PDFStreptococcus pyogenes infects human endometrium by limiting the innate immune response.
J Clin Invest
February 2021
Université de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR8104, Paris, France.
Group A Streptococcus (GAS), a Gram-positive human-specific pathogen, yields 517,000 deaths annually worldwide, including 163,000 due to invasive infections and among them puerperal fever. Before efficient prophylactic measures were introduced, the mortality rate for mothers during childbirth was approximately 10%; puerperal fever still accounts for over 75,000 maternal deaths annually. Yet, little is known regarding the factors and mechanisms of GAS invasion and establishment in postpartum infection.
View Article and Find Full Text PDFInvasive Infections in <3-Month-Old Infants in France: Clinical and Laboratory Features.
Front Pediatr
May 2020
Université de Paris, IAME, INSERM, Paris, France.
Few data are available on invasive group A (GAS) infections (IGASIs) in infants. We described initial clinical and laboratory features and outcomes of <3-month-old infants hospitalized for an IGASI between 2007 and 2016 in France. Patients were identified from the French National Reference Centre for streptococci.
View Article and Find Full Text PDFPermissive Fatty Acid Incorporation Promotes Staphylococcal Adaptation to FASII Antibiotics in Host Environments.
Cell Rep
December 2019
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy en Josas, France. Electronic address:
Article Synopsis
- - The study highlights the importance of fatty acid synthesis (FASII) products in Staphylococcus aureus, which has led to the development of antibiotics targeting this pathway, previously shown to work in animal models.
- - Researchers found that S. aureus can quickly adapt to FASII antibiotics when in host environments, without changing its FASII genes, and that administering these antibiotics during infection is less effective than expected.
- - The presence of serum in the host environment reduces the stress on S. aureus' membranes, allowing it to use alternative fatty acids and evade the effects of FASII antibiotics, indicating that this flexibility limits the effectiveness of these treatments alone.
Similarities and Differences Between Staphylococcal and Streptococcal Toxic Shock Syndromes in Children: Results From a 30-Case Cohort.
Front Pediatr
November 2018
Centre National de Référence des Staphylocoques, Institut des Agents Infectieux, Centre de Biologie et de Pathologie Nord, Hospices Civils de Lyon, Lyon, France.
Toxic shock syndromes (TSS) are severe shocks due to staphylococcal or streptococcal infection that require specific treatments. The early recognition of these shocks is crucial to improve their outcomes. The primary objective of this study was to compare characteristics and outcomes of staphylococcal and streptococcal TSS in children, in order to identify putative early clinical diagnostic criteria.
View Article and Find Full Text PDFInsights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells.
Microbes Infect
March 2019
Institut Pasteur, Biology of Gram-positive Pathogens Unit, 75015, Paris, France; Centre National de la Recherche Scientifique (CNRS) ERL6002, France. Electronic address:
The core PI-2b pilus present in "hypervirulent" ST-17 Streptococcus agalactiae strains consists of three pilin subunits (Spb1, Ap1 and Ap2) assembled by sortase SrtC1 and cell-wall anchored by Srt2. Spb1 was shown to be the major pilin and Ap2 the anchor pilin. Ap1 is a putative adhesin.
View Article and Find Full Text PDFMolecular epidemiology of invasive and non-invasive group B Streptococcus circulating in Serbia.
Int J Med Microbiol
January 2019
Institute of Microbiology and Immunology, Medical Faculty, University of Belgrade, Dr Subotica 1, 11000, Belgrade, Serbia; National Reference Laboratory for Streptococci, Dr Subotica 1, 11000, Belgrade, Serbia. Electronic address:
Streptococcus agalactiae (group B Streptococcus, GBS) remains the leading cause of invasive diseases in neonates and an important cause of infections in the elderly. The aim of this study was to access the prevalence of GBS genito-rectal colonisation of pregnant women and to evaluate the genetic characteristics of invasive and non-invasive GBS isolates recovered throughout Serbia. A total of 432 GBS isolates were tested for antimicrobial susceptibility, capsular polysaccharide (CPS) types and the presence of the hvgA gene.
View Article and Find Full Text PDFThe N-terminal domain of the R28 protein promotes group A adhesion to host cells via direct binding to three integrins.
J Biol Chem
October 2018
From the INSERM U1016, Institut Cochin,
Group A (GAS) is a human-specific pathogen responsible for a wide range of diseases, ranging from superficial to life-threatening invasive infections, including endometritis, and autoimmune sequelae. GAS strains express a vast repertoire of virulence factors that varies depending on the strain genotype, and many adhesin proteins that enable GAS to adhere to host cells are restricted to some genotypes. GAS is the third most prevalent genotype in invasive infections in France and is associated with gyneco-obstetrical infections.
View Article and Find Full Text PDFA clone of the emergent Streptococcus pyogenes emm89 clade responsible for a large outbreak in a post-surgery oncology unit in France.
Med Microbiol Immunol
November 2018
INSERM, U1016, Institut Cochin, 75014, Paris, France.
Article Synopsis
- * Researchers analyzed the genetic and phenotypic characteristics of GAS strains, finding that most belonged to the emerging emm89 clade 3, sharing similar molecular markers and displaying consistent biofilm formation and interactions with lung cells and immune cells.
- * The findings indicate that specific genetic mutations did not correlate with the severity of infections, highlighting the strain's ability to cause significant outbreaks among vulnerable patients due to its inherent phenotypic traits.
Colorectal cancer specific conditions promote gut colonization.
Proc Natl Acad Sci U S A
January 2018
Unité de Biologie des Bactéries Pathogènes à Gram-positif, Institut Pasteur, 75015 Paris, France;
Article Synopsis
- Colonization of the gut by a specific strain of bacteria (SGG) is linked to colorectal cancer (CRC), but the exact mechanisms behind this relationship are still unclear.
- Research using mice genetically prone to CRC reveals that concentrations of SGG are significantly higher in tumor-bearing mice compared to normal mice, likely due to SGG's ability to outcompete local bacteria (enterococci).
- The study identifies a specific gene in SGG that produces a bacteriocin, which is enhanced by bile acids, allowing SGG to kill enterococci, thus promoting its own colonization in the CRC-affected gut.
Intrapartum group B Streptococcus screening in the labor ward by Xpert® GBS real-time PCR.
Eur J Clin Microbiol Infect Dis
February 2018
AP-HP, Service de Bactériologie et Centre National de Référence des Streptocoques, Hôpitaux Universitaires Paris Centre site Cochin, Paris, France.
Group B Streptococcus (GBS) is the leading cause of neonatal infections in industrialized countries. Intrapartum antibiotic prophylaxis (IAP) given to colonized parturients is a key step for the prevention of neonatal early-onset infection. We compared the performances of Xpert® GBS polymerase chain reaction (PCR) (Cepheid, Sunnyvale, CA, USA) as a point-of-care system in labor wards to standard culture for intrapartum GBS detection.
View Article and Find Full Text PDFClinical and Laboratory Features of Group B Streptococcus Meningitis in Infants and Newborns: Study of 848 Cases in France, 2001-2014.
Clin Infect Dis
March 2018
Université Paris Est, IMRB-GRC GEMINI, Créteil, France.
Background: Group B Streptococcus (GBS) disease is the leading cause of neonatal bacterial meningitis despite women receiving an intravenous antibiotic prophylaxis during labor. We aimed to describe GBS meningitis in children <1 year old in France.
Methods: Clinical and biological data of GBS meningitis gathered by the Association Clinique et Thérapeutique Infantile du Val de Marne (ACTIV) were analyzed.