Engineering proteins with catechol chemistry for biotechnological applications.

Developing proteins with increased chemical space by expanding the amino acids alphabet has been an emerging technique to compete for the obstacle encountered by their need in various applications. 3,4-Dihydroxyphenylalanine (L-DOPA) catecholic unnatural amino acid is abundantly present in mussels foot proteins through post-translational modification of tyrosine to give a strong adhesion toward wet rocks. L-DOPA forms: bidentate coordination, H-bonding, metal-ligand complexes, long-ranged electrostatic, and van der Waals interactions a pair of donor hydroxyl groups.

C CSA Tensors and Orientational Order of Model and Dimer Mesogens Comprising of Phenyl Benzoate.

A Model mesogen and its symmetrical Dimer made up of phenyl benzoate core unit are investigated by C NMR spectroscopy. The existence of layer order in smectic A and smectic C phases of Dimer mesogen is established by powder X-ray diffraction. The chemical shift anisotropy (CSA) tensors of Model mesogen are determined by 2D separation of undistorted powder patterns by effortless recoupling (SUPER) experiment and are utilized for calculating the order parameters employing the alignment-induced chemical shifts (AIS).

Expanding limits of artificial enzymes: unprecedented catalysis by an oxidase nanozyme in activating a structural protein for covalent crosslinking and conferring remarkable proteolytic resistance.

Nature has endowed us with some complex enzymes capable of utilizing proteins as their substrates to generate functional proteins through post-translational modification. However, nanozymes' interplay with proteins as substrates is scarce, with their chemistry predominantly established using only small molecule substrates, featuring a significant gap in this area. Due to the huge prospects of nanozymes in biotechnological and therapeutic interventions, studies establishing the unexplored roles of nanozymes in the biological environments and their interplay beyond small molecule substrates warrant immediate attention.

An anionic two dimensional covalent organic framework from tetratopic borate centres pillared by lithium ions.

Non-covalent interactions play an important role for the framework formation of two-dimensional covalent organic frameworks. Until now, π-π interactions and hydrogen bonding are the main reported forces facilitating the stacking of framework layers. Here, we present a two-dimensional anionic covalent organic framework based on tetratopic borate linkages, where layers are connected by ionic interactions between the linkage site and counter cations.

Cyt b gene as a valid molecular authentication marker of cow leathers.

Authentication of true (genuine) cow leathers is in high demand to promote merchandise and economic growth. The present study employs RT-PCR-based TaqMan assay to facilitate the identification. Species-specific primers and probes were designed utilizing the existing NCBI data on mitochondrial DNA (mtDNA) genes, particularly the cytochrome b region (Cyt b).

Identifying the molecular mechanisms of action of wound healing properties of Mathan tailam and Mahamegarajanga tailam in Wistar albino rats: Evidence through IL-10/VEGF/TNF-α signalling pathways.

The Siddha system of medicine (SSM) is the oldest medical science practised in the ancient period of the southern part of India and Sri Lanka. Many formulations were described for wound healing in the SSM, with specific diagnostic differentiation in the Siddha literature. Most preparations for wound healing were available in the form of oil-based formulations, especially for external usage.

Combinatorial effect of Apigenin-resveratrol on white adipocyte plasticity and trans-differentiation for activating lipid metabolism.

Inducing browning in white adipocytes has emerged as a promising therapeutic approach for addressing obesity. Bioactive that modulate the WAT microenvironment to induce trans browning in white adipocytes have been explored as a strategy to control unregulated lipid storage. However, relying on a single bioactive for modulating lipid metabolism has proven insufficient in obese individuals during human trials, because these compounds primarily activate a single biochemical pathway in promoting browning.

Comprehensive review on collagen extraction from food by-products and waste as a value-added material.

The consumption of animal products has witnessed a significant increase over the years, leading to a growing need for industries to adopt strict waste control measures to mitigate environmental impacts. The disposal of animal waste in landfill can result in diverse and potentially hazardous decomposition by-products. Animal by-products, derived from meat, poultry, seafood and fish industries, offer a substantial raw material source for collagen and gelatin production due to their high protein content.

An attention-based deep learning for acute lymphoblastic leukemia classification.

The bone marrow overproduces immature cells in the malignancy known as Acute Lymphoblastic Leukemia (ALL). In the United States, about 6500 occurrences of ALL are diagnosed each year in both children and adults, comprising nearly 25% of pediatric cancer cases. Recently, many computer-assisted diagnosis (CAD) systems have been proposed to aid hematologists in reducing workload, providing correct results, and managing enormous volumes of data.

Approach of a small protein to the biomimetic bis-(μ-oxo) dicopper active-site installed in MOF-808 pores with restricted access perturbs substrate selectivity of oxidase nanozyme.

Advances in nanozymes have taken shape over the past few years in several domains. However, persisting challenging limitations of selectivity, specificity, and efficiency necessitate careful attention to aid in the development of next-generation artificial enzymes. Despite nanozymes having significant therapeutic and biotechnological prospects, the multienzyme mimetic activities can compromise their intended applications.

Green Nanoengineered Fabrics: Waste-Derived Polyphenol-Zinc@ Silica Core-Shell Reactive Janus Nanoparticles for Functional Fabrics.

Fabricating Janus nanoparticle-functionalized fabrics with UV protection, strength enhancement, self-cleaning properties, and wash durability, with a biocompatible nature, is crucial in modern functional fabrics engineering. Particularly, tailoring multifunctional nanoparticles capable of exhibiting several distinct properties, utilizing low-cost raw materials, and adhering to green chemistry principles is pivotal. A fabrication strategy for developing multifunctional reactive Janus nanoparticles, utilizing waste-derived natural polyphenol (quercetin-3-glucuronide, myricetin-3-galactoside, gossypin, phlorizin, kaempferol, myricetin-3-arabinoside)-integrated zinc-silica core-shell Janus nanoparticles with UV protection, strength enhancement, and self-cleaning properties, is proposed.

Hierarchical Self-Assembly and Aggregation-Induced Emission Enhancement in Tetrabenzofluorene-Based Red Emitting Molecules.

The newly synthesized chiral active [5]helicene-like tetrabenzofluorene (TBF) based highly red-emitting molecules exhibit flower-like self-assembly. These molecules display photophysical and structural properties such as intramolecular charge transfer, dual state emission, large fluorescence quantum yield, and solvatochromism. In TBFID, the indandione functional group attached on both sides as the terminal group offers an A-D-A push-pull effect and acts as a strong acceptor to cause more redshift in solution as well as in solid state as compared to TBFPA (TBF with benzaldehyde functional group in terminal position).

Antibiotic Indexing and Heavy Metal Reduction Potential of Four Multi-metal Tolerant Bacterial Strains in Real-Time Sanitary Landfill Leachate Matrix.

Sanitary leachate from urban landfills is known to be contaminated with multi-metals and residual antibiotics. Current research edges on exploring the multi-metal and antibiotic sensitivity profile of four indigenous strains, "Brevibacillus spp. Leclercia spp.

Microbially induced carbonate precipitation with Arthrobacter creatinolyticus: An eco-friendly strategy for mitigation of chromium contamination.

Chromium contamination from abandoned industrial sites and inadequately managed waste disposal areas poses substantial environmental threat. Microbially induced carbonate precipitation (MICP) has shown promising, eco-friendly solution to remediate Cr(VI) and divalent heavy metals. In this study, MICP was carried out for chromium immobilization by an ureolytic bacterium Arthrobacter creatinolyticus which is capable of reducing Cr(VI) to less toxic Cr(III) via extracellular polymeric substances (EPS) production.

In vitro and in silico approach towards antimicrobial and antioxidant behaviour of water-soluble chitosan dialdehyde biopolymers.

Chitosan dialdehyde (ChDA) was prepared from a three-step process initiated by thermal organic acid hydrolysis, periodate oxidization, and precipitation from native chitosan (NCh). The developed ChDA resulted in an aldehydic content of about 82 % with increased solubility (89 %) and maximum yield (97 %). The functional alteration of the aldehydic (-CHO) group in ChDA was established using vibrational stretching at 1744 cm.

Lutein, a carotenoid found in numerous plants and the human eye, demonstrates the capacity to bundle collagen fibrils.

Collagen fibrils serve as the building blocks of the extracellular matrix, providing a resilient and structural framework for tissues. However, the bundling of collagen fibrils is of paramount importance in maintaining the structural integrity and functionality of various tissues in the human body. In this scenario, there is limited exploration of molecules that promote the bundling of collagen fibrils.

Valorization of agitated thin film dryer (ATFD)-mixed-salt from textile wastewater for its application in leather manufacturing - a sustainable approach.

The textile industry uses sodium chloride and sodium sulphate during the dyeing process to improve the fixation of dyes on fabrics. After wastewater treatment, the reject stream is dried resulting in mixed salts as solid wastes that are not reused. The leather industry also uses a vast quantity of salt for temporary preservation of skins/hides and as swelling-suppressing agent during the pickling (acidification) process.

Bayesian Optimization for Efficient Prediction of Gas Uptake in Nanoporous Materials.

The discovery and optimization of novel nanoporous materials (NPMs) such as Metal-Organic Frameworks (MOFs) and Covalent Organic Frameworks (COFs) are crucial for addressing global challenges like climate change, energy security, and environmental degradation. Traditional experimental approaches for optimizing these materials are time-consuming and resource-intensive. This research paper presents a strategy using Bayesian optimization (BO) to efficiently navigate the complex design spaces of NPMs for gas storage applications.

Design of Isoindigo-Based Small-Molecule Donors for Bulk Heterojunction Organic Solar Cell Applications in Combination with Nonfullerene Acceptors.

The development of small-molecule organic solar cells with the required efficiency depends on the information obtained from molecular-level studies. In this context, 39 small-molecule donors featuring isoindigo as an acceptor moiety have been meticulously crafted for potential applications in bulk heterojunction organic solar cells. These molecules follow the D-A-D-A-D and D-A-π-D-π-A-D framework.

Genetically encoded biocompatible anti-coagulant protein-coated coronary artery stents drive endothelialization.

In response to the critical demand for advancements in coronary artery stents, this study addresses the challenges associated with arterial recoil and restenosis post-angioplasty and the imperative to encourage rapid re-endothelialization for minimizing thrombosis risks. We employed an innovative approach inspired by mussel adhesion, incorporating placental anticoagulant protein (AnnexinV) on stent design. The introduction of a post-translationally modified catecholic amino acid L-3,4-dihydroxyphenylalanine (L-Dopa), mimicking mussel characteristics, allowed for effective surface modification of Stainless steel stents through genetic code engineering in AnnexinV (AnxDopa).

A fluorescent chemosensor for selective detection of chromium (III) ions in environmentally and biologically relevant samples.

A simple single step one pot multicomponent reaction was performed to synthesize N-(tert-butyl)-2-(furan-2-yl)imidazo[1,2-a]pyridine-3-amine (TBFIPA). The synthesized TBFIPA was subjected to library of cations to study its ability for selective and sensitive detection of specific metal ions. Selective detection of chromium ions by TBFIPA were found from the significant hypsochromic shift (335 nm → 285 nm) in the UV-Visible spectra.

Exploring Crystal-Phase Molecular Dynamics of the Low-Viscous Mesogen 6CHBT: A Combined FFC and High-Field NMR Relaxometry Investigation.

The molecular dynamics study of thermotropic mesogens exhibiting the crystal phases is valuable in unraveling the complex global (collective) and local (noncollective) motions executed by liquid crystal molecules, which would further advance the existing knowledge on orientationally disordered crystalline (ODIC) phases. Toward the fulfillment of such a task, a combined nuclear magnetic resonance (NMR) relaxometry approach employing the fast field cycling (FFC) NMR (10 kHz-30 MHz) and high-field pulsed NMR (400 MHz) techniques is utilized to sample the broad frequency range offered by molecular motions in the crystal phase of 4-(trans-4'--hexylcyclohexyl)-isothiocyanatobenzene (6CHBT). The validity of the observed relaxation data is tested and interpreted by the Bloembergen-Purcell-Pound (BPP) model involving the superposition of four mutually independent Lorentzian spectral densities, reflecting molecular dynamical processes on different time scales.

Incorporating silver nanoparticles into electrospun nanofibers of casein/polyvinyl alcohol to develop scaffolds for tissue engineering.

Developing novel antimicrobial wound dressings that have the potential to address the challenges associated with chronic wounds is highly imperative in providing effective infection control and wound healing support. Biocompatible electrospun nanofibers with their high porosity and surface area enabling efficient drug loading and delivery have been investigated in this regard as viable candidates for chronic wound care. Here, we design Casein/Polyvinyl alcohol (CAN/PVA) nanofibers reinforced with silver nanoparticles (Ag NPs) by the electrospinning technique to develop diabetic wound healing scaffolds.

Harnessing halogen bond donors for enhanced nitrogen reduction: a case study on metal-free boron nitride single-atom catalysts.

Developing efficient catalysts for ammonia synthesis is increasingly crucial but remains a formidable challenge due to the lack of robust design criteria, particularly in addressing the activity and selectivity issues, especially in electrochemical nitrogen reduction reactions (NRR). In this study, we systematically investigated the catalytic potential of hexagonal boron nitride (BN) embedded with non-metal (C, Si, P and S) atoms as an electrocatalyst for the nitrogen reduction reaction using density functional theory (DFT) computations. The preference for non-metal-doped BN nanomaterials stems from their ability to suppress hydrogen evolution and their environmentally friendly nature, in contrast to transition metals.

Photocrosslinkable triple helical protein with enhanced higher-order formation for biomaterial applications.

Bacterial collagen, produced via recombinant DNA methods, offers advantages including consistent purity, customizable properties, and reduced allergy potential compared to animal-derived collagen. Its controlled production environment enables tailored features, making it more sustainable, non-pathogenic, and compatible with diverse applications in medicine, cosmetics, and other industries. Research has focused on the engineering of collagen-like proteins to improve their structure and function.