Specific antitumor effects of tumor vaccine produced by electrofusion between osteosarcoma cell and dendritic cell in rats.

Dendritic cells (DCs) are potent antigen-presenting cells capable of inducing primary T-cell responses. Several immunotherapy treatment strategies involve manipulation of DCs, both in vivo and ex vivo, to promote the immunogenic presentation of tumor-associated antigens. In this study, an electrofusion protocol was developed to induce fusion between osteosarcoma cells and allogeneic bone marrow-derived DCs.

[Inducing specific antitumor effects on osteosarcoma by fused vaccine of rat dendritic cells].

Background & Objective: Dendritic cells (DCs) are the strongest antigen presenting cells (APCs). But immune therapy based on DCs for osteosarcoma has seldom been reported. This study was designed to investigate the immunologic potency and antitumor effects of fused vaccine of rat DCs on osteosarcoma.

[Application of repairing tibia and soft tissue defect with free fibula combined tissue grafting].

Objective: To investigate a good method for repairing the long bone defect of tibia combined with soft tissue defect.

Methods: From 1988-1998, sixteen patients with long bone defect of tibia were admitted. There were 12 males, 4 females and aged from 16 to 45 years.

[Experimental study of effect of meniscus suture on meniscus healing].

Objective: To investigate the effect of meniscus suture on meniscus healing which included healing time and healing pattern.

Methods: Fourty healthy rabbits were adopted in this study. The model of meniscus injury was made by a longitudinal incision at the medial meniscus of the left knee.

[Double semitendinous reconstruction of posterior cruciate ligament with invasive mini-plate technique].

Objective: To probe the clinical results of a new designed operation-double semitendinous reconstruction of posterior cruciate ligament (PCL) with invasive mini-plate.

Methods: The new surgical technique was performed on 28 patients with PCL deficient knee in our department from September 1994 to October 1997. Protection of popliteal nerves and blood vessels was emphasized in the operation, and the femoral and tibial tunnel placement was critical to the procedure's success.

Evaluation of transforming growth factor beta and bone morphogenetic protein composite on healing of bone defects.

Objective: To evaluate the effect of transforming growth factor-beta/bone morphogenetic protein (TGF-beta/BMP) composite on the healing of large segmental bone defects and to discuss the interaction between TGF-beta and BMP during bone repair.

Methods: A 1.5 cm segmental defect was made in the mid-upper part of the radial shaft of 48 adult rabbits.